Restriction map for
pF1KE0787
(Circular) (Six-base) MAPSORT of: pF1KE0787.seq Check: 4889 from: 1 to: 4172
pF1KE0787 4172 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 292
Size: 4172
AccI GT'mk_AC
Cuts at: 1210 1210
Size: 4172
Acc65I G'GTAC_C
Cuts at: 1188 1188
Size: 4172
AclI AA'CG_TT
Cuts at: 4012 4012
Size: 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1997 2429 3176 3444 349
Size: 1648 432 747 268 1077
Fragments arranged by size:
1648 1077 747 432 268
AflIII A'CryG_T
Cuts at: 1524 2628 3605 1524
Size: 1104 977 2091
Fragments arranged by size:
2091 1104 977
AgeI A'CCGG_T
Cuts at: 2560 2560
Size: 4172
AleI CACnn'nnGTG
Cuts at: 205 850 205
Size: 645 3527
AlwNI CAG_nnn'CTG
Cuts at: 447 747 1477 3044 447
Size: 300 730 1567 1575
Fragments arranged by size:
1575 1567 730 300
ApaLI G'TGCA_C
Cuts at: 2942 2942
Size: 4172
ApoI r'AATT_y
Cuts at: 1176 3525 3781 1176
Size: 2349 256 1567
Fragments arranged by size:
2349 1567 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
AvaI C'yCGr_G
Cuts at: 371 1192 371
Size: 821 3351
BamHI G'GATC_C
Cuts at: 257 927 1197 257
Size: 670 270 3232
Fragments arranged by size:
3232 670 270
BanI G'GyrC_C
Cuts at: 1188 1602 1850 1885 1188
Size: 414 248 35 3475
Fragments arranged by size:
3475 414 248 35
BanII G_rGCy'C
Cuts at: 1186 2216 1186
Size: 1030 3142
BbeI G_GCGC'C
Cuts at: 1606 1854 1606
Size: 248 3924
BbsI GAAGACnn'nnnn_
Cuts at: 337 337
Size: 4172
BciVI GTATCCnnnnn_n'
Cuts at: 2070 2831 4172 2070
Size: 761 1341 2070
Fragments arranged by size:
2070 1341 761
BclI T'GATC_A
Cuts at: 1692 1692
Size: 4172
BglII A'GATC_T
Cuts at: 1687 3724 1687
Size: 2037 2135
BlpI GC'TnA_GC
Cuts at: 1127 1284 1127
Size: 157 4015
Bme1580I G_kGCm'C
Cuts at: 1797 1890 2946 1797
Size: 93 1056 3023
Fragments arranged by size:
3023 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1049 1384 1465 1800 3944 1049
Size: 335 81 335 2144 1277
Fragments arranged by size:
2144 1277 335 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 379 661 316
Size: 63 282 3827
Fragments arranged by size:
3827 282 63
Bpu10I CC'TnA_GC
Cuts at: 823 823
Size: 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 241 1348 2227 2719 3017 109
Size: 132 1107 879 492 298 1264
Fragments arranged by size:
1264 1107 879 492 298 132
BsaI GGTCTCn'nnnn_
Cuts at: 3876 3876
Size: 4172
BsaAI yAC'GTr
Cuts at: 1525 1525
Size: 4172
BsaBI GATnn'nnATC
Cuts at: 6 1686 3723 6
Size: 1680 2037 455
Fragments arranged by size:
2037 1680 455
BsaHI Gr'CG_yC
Cuts at: 1603 1851 2553 4049 1603
Size: 248 702 1496 1726
Fragments arranged by size:
1726 1496 702 248
BsaWI w'CCGG_w
Cuts at: 529 1361 1882 2560 2834 2981 3392 529
Size: 832 521 678 274 147 411 1309
Fragments arranged by size:
1309 832 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 391 582 688 307
Size: 84 191 106 3791
Fragments arranged by size:
3791 191 106 84
BseYI C'CCAG_C
Cuts at: 1597 2355 2932 3428 1597
Size: 758 577 496 2341
Fragments arranged by size:
2341 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 433 258
Size: 175 3997
BsiEI CG_ry'CG
Cuts at: 104 313 1714 1760 2968 104
Size: 209 1401 46 1208 1308
Fragments arranged by size:
1401 1308 1208 209 46
BsiHKAI G_wGCw'C
Cuts at: 125 829 1186 1964 2154 2946 125
Size: 704 357 778 190 792 1351
Fragments arranged by size:
1351 792 778 704 357 190
Bsp1286I G_dGCh'C
Cuts at: 125 829 1186 1797 1890 1964 2154 2216 2946
Size: 704 357 611 93 74 190 62 730
Cuts at: 2946 125
Size: 1351
Fragments arranged by size:
1351 730 704 611 357 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3392 3392
Size: 4172
BspHI T'CATG_A
Cuts at: 3348 3348
Size: 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 1222 1436 1738 2119 292
Size: 930 214 302 381 2345
Fragments arranged by size:
2345 930 381 302 214
BsrBI CCG'CTC
Cuts at: 50 315 381 2464 2518 4002 4167 50
Size: 265 66 2083 54 1484 165 55
Fragments arranged by size:
2083 1484 265 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 2084 2084
Size: 4172
BsrFI r'CCGG_y
Cuts at: 2170 2351 2560 2170
Size: 181 209 3782
Fragments arranged by size:
3782 209 181
BsrGI T'GTAC_A
Cuts at: 767 767
Size: 4172
BssSI C'ACGA_G
Cuts at: 1006 2443 2801 1006
Size: 1437 358 2377
Fragments arranged by size:
2377 1437 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1549 1549
Size: 4172
BstBI TT'CG_AA
Cuts at: 2533 2533
Size: 4172
BstEII G'GTnAC_C
Cuts at: 648 648
Size: 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 639 669 639
Size: 30 4142
BstYI r'GATC_y
Cuts at: 1 257 729 927 1197 1664 1687 2022 2268
Size: 256 472 198 270 467 23 335 246
Cuts at: 2268 3280 3366 3378 3724 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 270 256
246 198 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 685 685
Size: 4172
BtgI C'CryG_G
Cuts at: 107 344 107
Size: 237 3935
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 481 2122 2303 2466 192
Size: 289 1641 181 163 1898
Fragments arranged by size:
1898 1641 289 181 163
BtsI GCAGTG_nn'
Cuts at: 1415 1415
Size: 4172
DraI TTT'AAA
Cuts at: 1171 1171
Size: 4172
DrdI GACnn_nn'nnGTC
Cuts at: 1878 2736 1878
Size: 858 3314
EaeI y'GGCC_r
Cuts at: 629 734 869 1757 1931 2322 2349 3694 629
Size: 105 135 888 174 391 27 1345 1107
Fragments arranged by size:
1345 1107 888 391 174 135 105 27
EagI C'GGCC_G
Cuts at: 1757 1757
Size: 4172
EarI CTCTTCn'nnn_
Cuts at: 959 998 1075 2195 2405 959
Size: 39 77 1120 210 2726
Fragments arranged by size:
2726 1120 210 77 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2380 2690 2836 3982 2380
Size: 310 146 1146 2570
Fragments arranged by size:
2570 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1184 1184
Size: 4172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 349 379 661 1997 2429 3176 3444 316
Size: 33 30 282 1336 432 747 268 1044
Fragments arranged by size:
1336 1044 747 432 282 268 33 30
EcoO109I rG'GnC_Cy
Cuts at: 1311 1311
Size: 4172
EcoRI G'AATT_C
Cuts at: 1176 1176
Size: 4172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 2191 150
Size: 2041 2131
FspI TGC'GCA
Cuts at: 1953 1953
Size: 4172
HaeII r_GCGC'y
Cuts at: 1606 1854 2876 3855 1606
Size: 248 1022 979 1923
Fragments arranged by size:
1923 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 302 334 2014 2044 2046 2076 170
Size: 32 100 32 1680 30 2 30 2266
Fragments arranged by size:
2266 1680 100 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1211 1211
Size: 4172
HindIII A'AGCT_T
Cuts at: 217 217
Size: 4172
KasI G'GCGC_C
Cuts at: 1602 1850 1602
Size: 248 3924
KpnI G_GTAC'C
Cuts at: 1192 1192
Size: 4172
MluI A'CGCG_T
Cuts at: 3605 3605
Size: 4172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 211 641 2843 3027 211
Size: 430 2202 184 1356
Fragments arranged by size:
2202 1356 430 184
MscI TGG'CCA
Cuts at: 871 1933 871
Size: 1062 3110
MslI CAynn'nnrTG
Cuts at: 205 667 850 947 2161 2288 205
Size: 462 183 97 1214 127 2089
Fragments arranged by size:
2089 1214 462 183 127 97
MspA1I CmG'CkG
Cuts at: 447 1283 1597 1957 2970 3215 447
Size: 836 314 360 1013 245 1404
Fragments arranged by size:
1404 1013 836 360 314 245
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 4172
NarI GG'CG_CC
Cuts at: 1603 1851 1603
Size: 248 3924
NcoI C'CATG_G
Cuts at: 107 107
Size: 4172
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 4172
NspI r_CATG'y
Cuts at: 1225 1725 2632 1225
Size: 500 907 2765
Fragments arranged by size:
2765 907 500
PciI A'CATG_T
Cuts at: 2628 2628
Size: 4172
PflMI CCAn_nnn'nTGG
Cuts at: 982 982
Size: 4172
PfoI T'CCnGG_A
Cuts at: 263 356 746 263
Size: 93 390 3689
Fragments arranged by size:
3689 390 93
PmeI GTTT'AAAC
Cuts at: 1171 1171
Size: 4172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 4140
PstI C_TGCA'G
Cuts at: 1219 1904 1219
Size: 685 3487
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4172
PvuII CAG'CTG
Cuts at: 447 1597 1957 447
Size: 1150 360 2662
Fragments arranged by size:
2662 1150 360
RsrII CG'GwC_CG
Cuts at: 2367 2367
Size: 4172
SacI G_AGCT'C
Cuts at: 1186 1186
Size: 4172
SalI G'TCGA_C
Cuts at: 1209 1209
Size: 4172
SapI GCTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
SbfI CC_TGCA'GG
Cuts at: 1219 1219
Size: 4172
ScaI AGT'ACT
Cuts at: 352 3689 352
Size: 3337 835
SfcI C'TryA_G
Cuts at: 33 1215 1900 2893 3084 33
Size: 1182 685 993 191 1121
Fragments arranged by size:
1182 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1604 1852 1604
Size: 248 3924
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
SmaI CCC'GGG
Cuts at: 1194 1194
Size: 4172
SmlI C'TyrA_G
Cuts at: 124 220 371 1327 2242 2734 2996 124
Size: 96 151 956 915 492 262 1300
Fragments arranged by size:
1300 956 915 492 262 151 96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4172
SphI G_CATG'C
Cuts at: 1225 1725 1225
Size: 500 3672
StuI AGG'CCT
Cuts at: 364 690 364
Size: 326 3846
StyI C'CwwG_G
Cuts at: 107 248 806 1306 1660 107
Size: 141 558 500 354 2619
Fragments arranged by size:
2619 558 500 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 685 970 1755 1888 2347 2556 685
Size: 285 785 133 459 209 2301
Fragments arranged by size:
2301 785 459 285 209 133
TatI w'GTAC_w
Cuts at: 209 350 767 3687 209
Size: 141 417 2920 694
Fragments arranged by size:
2920 694 417 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4140
Tth111I GACn'n_nGTC
Cuts at: 1969 1969
Size: 4172
XbaI T'CTAG_A
Cuts at: 1203 1203
Size: 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1667 1667
Size: 4172
XhoI C'TCGA_G
Cuts at: 371 371
Size: 4172
XmaI C'CCGG_G
Cuts at: 1192 1192
Size: 4172
XmnI GAAnn'nnTTC
Cuts at: 537 537
Size: 4172
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI