Restriction map for
pF1KE0795
(Circular) (Six-base) MAPSORT of: pF1KE0795.seq Check: 1505 from: 1 to: 4187
pF1KE0795 4187 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 197 318 197
Size: 121 4066
AccI GT'mk_AC
Cuts at: 380 1045 1225 380
Size: 665 180 3342
Fragments arranged by size:
3342 665 180
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1155 2012 2444 3191 3459 662
Size: 493 857 432 747 268 1390
Fragments arranged by size:
1390 857 747 493 432 268
AflIII A'CryG_T
Cuts at: 1539 2643 3620 1539
Size: 1104 977 2106
Fragments arranged by size:
2106 1104 977
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AhdI GACnn_n'nnGTC
Cuts at: 753 753
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 1492 3059 1492
Size: 1567 2620
ApaI G_GGCC'C
Cuts at: 589 693 1110 1137 589
Size: 104 417 27 3639
Fragments arranged by size:
3639 417 104 27
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 999 1191 3540 3796 999
Size: 192 2349 256 1390
Fragments arranged by size:
2349 1390 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 385 1207 385
Size: 822 3365
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 132 1203 1617 1865 1900 132
Size: 1071 414 248 35 2419
Fragments arranged by size:
2419 1071 414 248 35
BanII G_rGCy'C
Cuts at: 239 589 686 693 949 1110 1137 1201 2231
Size: 350 97 7 256 161 27 64 1030
Cuts at: 2231 239
Size: 2195
Fragments arranged by size:
2195 1030 350 256 161 97 64 27
7
BbeI G_GCGC'C
Cuts at: 136 1621 1869 136
Size: 1485 248 2454
Fragments arranged by size:
2454 1485 248
BbvCI CC'TCA_GC
Cuts at: 671 671
Size: 4187
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 256 593 766 256
Size: 337 173 3677
Fragments arranged by size:
3677 337 173
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 589 693 881 1110 1137 1812 1905 2961 589
Size: 104 188 229 27 675 93 1056 1815
Fragments arranged by size:
1815 1056 675 229 188 104 93 27
BmgBI CAC'GTC
Cuts at: 750 750
Size: 4187
BmrI ACTGGGnnnn_n'
Cuts at: 584 1399 1480 1815 3959 584
Size: 815 81 335 2144 812
Fragments arranged by size:
2144 815 812 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 2554 258
Size: 2296 1891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 960 1170 178
Size: 782 210 3195
Fragments arranged by size:
3195 782 210
Bpu10I CC'TnA_GC
Cuts at: 671 671
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 2242 2734 3032 1363
Size: 879 492 298 2518
Fragments arranged by size:
2518 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 310 775 3891 310
Size: 465 3116 606
Fragments arranged by size:
3116 606 465
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 133 416 1618 1866 2568 4064 133
Size: 283 1202 248 702 1496 256
Fragments arranged by size:
1496 1202 702 283 256 248
BsaWI w'CCGG_w
Cuts at: 644 1376 1897 2575 2849 2996 3407 644
Size: 732 521 678 274 147 411 1424
Fragments arranged by size:
1424 732 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 4157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 1006 1041 1044 445
Size: 561 35 3 3588
Fragments arranged by size:
3588 561 35 3
BseYI C'CCAG_C
Cuts at: 1612 2370 2947 3443 1612
Size: 758 577 496 2356
Fragments arranged by size:
2356 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 511 595 175
Size: 336 84 3767
Fragments arranged by size:
3767 336 84
BsiEI CG_ry'CG
Cuts at: 104 128 251 479 1729 1775 2983 104
Size: 24 123 228 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 228 123 46 24
BsiHKAI G_wGCw'C
Cuts at: 857 1201 1979 2169 2961 857
Size: 344 778 190 792 2083
Fragments arranged by size:
2083 792 778 344 190
BsmBI CGTCTCn'nnnn_
Cuts at: 382 382
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 239 589 686 693 857 881 949 1110 1137
Size: 350 97 7 164 24 68 161 27
Cuts at: 1137 1201 1812 1905 1979 2169 2231 2961 239
Size: 64 611 93 74 190 62 730 1465
Fragments arranged by size:
1465 730 611 350 190 164 161 97
93 74 68 64 62 27 24 7
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 318 617 1237 1451 1753 2134 197
Size: 121 299 620 214 302 381 2250
Fragments arranged by size:
2250 620 381 302 299 214 121
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 246 278 474 2185 2366 2575 246
Size: 32 196 1711 181 209 1858
Fragments arranged by size:
1858 1711 209 196 181 32
BssHII G'CGCG_C
Cuts at: 441 441
Size: 4187
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 792 792
Size: 4187
BstYI r'GATC_y
Cuts at: 1 967 1212 1679 1702 2037 2283 3295 3381
Size: 966 245 467 23 335 246 1012 86
Cuts at: 3381 3393 3739 1
Size: 12 346 449
Fragments arranged by size:
1012 966 467 449 346 335 246 245
86 23 12
BtgI C'CryG_G
Cuts at: 1091 1091
Size: 4187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 125 248 476 1088 1094 1772 1946 2337 2364
Size: 123 228 612 6 678 174 391 27
Cuts at: 2364 3709 125
Size: 1345 603
Fragments arranged by size:
1345 678 612 603 391 228 174 123
27 6
EagI C'GGCC_G
Cuts at: 125 248 476 1772 125
Size: 123 228 1296 2540
Fragments arranged by size:
2540 1296 228 123
EarI CTCTTCn'nnn_
Cuts at: 1174 2210 2420 1174
Size: 1036 210 2941
Fragments arranged by size:
2941 1036 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 747 960 2395 2705 2851 3997 747
Size: 213 1435 310 146 1146 937
Fragments arranged by size:
1435 1146 937 310 213 146
EcoICRI GAG'CTC
Cuts at: 1199 1199
Size: 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 662 960 1155 1170 2012 2444 3191 3459
Size: 484 298 195 15 842 432 747 268
Cuts at: 3459 178
Size: 906
Fragments arranged by size:
906 842 747 484 432 298 268 195
15
EcoNI CCTnn'n_nnAGG
Cuts at: 160 928 160
Size: 768 3419
EcoO109I rG'GnC_Cy
Cuts at: 585 586 1106 1134 1326 585
Size: 1 520 28 192 3446
Fragments arranged by size:
3446 520 192 28 1
EcoRI G'AATT_C
Cuts at: 999 1191 999
Size: 192 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 136 457 1039 1621 1869 2891 3870 136
Size: 321 582 582 248 1022 979 453
Fragments arranged by size:
1022 979 582 582 453 321 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 678 739 771 831 863 2029 2059 2061
Size: 32 61 32 60 32 1166 30 2
Cuts at: 2061 2091 646
Size: 30 2742
Fragments arranged by size:
2742 1166 61 60 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 381 1226 381
Size: 845 3342
KasI G'GCGC_C
Cuts at: 132 1617 1865 132
Size: 1485 248 2454
Fragments arranged by size:
2454 1485 248
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1082 2858 3042 1082
Size: 1776 184 2227
Fragments arranged by size:
2227 1776 184
MscI TGG'CCA
Cuts at: 1090 1096 1948 1090
Size: 6 852 3329
Fragments arranged by size:
3329 852 6
MslI CAynn'nnrTG
Cuts at: 860 2176 2303 860
Size: 1316 127 2744
Fragments arranged by size:
2744 1316 127
MspA1I CmG'CkG
Cuts at: 124 402 1298 1612 1972 2985 3230 124
Size: 278 896 314 360 1013 245 1081
Fragments arranged by size:
1081 1013 896 360 314 278 245
NaeI GCC'GGC
Cuts at: 248 476 2368 248
Size: 228 1892 2067
Fragments arranged by size:
2067 1892 228
NarI GG'CG_CC
Cuts at: 133 1618 1866 133
Size: 1485 248 2454
Fragments arranged by size:
2454 1485 248
NcoI C'CATG_G
Cuts at: 1091 1091
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 246 474 2366 246
Size: 228 1892 2067
Fragments arranged by size:
2067 1892 228
NruI TCG'CGA
Cuts at: 521 521
Size: 4187
NspI r_CATG'y
Cuts at: 1240 1740 2647 1240
Size: 500 907 2780
Fragments arranged by size:
2780 907 500
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PflMI CCAn_nnn'nTGG
Cuts at: 330 330
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PspOMI G'GGCC_C
Cuts at: 585 689 1106 1133 585
Size: 104 417 27 3639
Fragments arranged by size:
3639 417 104 27
PstI C_TGCA'G
Cuts at: 407 1234 1919 407
Size: 827 685 2675
Fragments arranged by size:
2675 827 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 402 1612 1972 402
Size: 1210 360 2617
Fragments arranged by size:
2617 1210 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1201 1201
Size: 4187
SalI G'TCGA_C
Cuts at: 379 1224 379
Size: 845 3342
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 403 1230 1915 2908 3099 33
Size: 370 827 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 370 191
SfoI GGC'GCC
Cuts at: 134 1619 1867 134
Size: 1485 248 2454
Fragments arranged by size:
2454 1485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 1342 2257 2749 3011 1342
Size: 915 492 262 2518
Fragments arranged by size:
2518 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StyI C'CwwG_G
Cuts at: 938 1091 1321 1675 938
Size: 153 230 354 3450
Fragments arranged by size:
3450 354 230 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 218 3702 218
Size: 3484 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 792 957 1682 792
Size: 165 725 3297
Fragments arranged by size:
3297 725 165
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI