Restriction map for
pF1KE0801
(Circular) (Six-base) MAPSORT of: pF1KE0801.seq Check: 1247 from: 1 to: 4181
pF1KE0801 4181 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 2006 2438 3185 3453 128
Size: 1878 432 747 268 856
Fragments arranged by size:
1878 856 747 432 268
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AleI CACnn'nnGTG
Cuts at: 1075 1075
Size: 4181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 188
Size: 32 4149
AlwNI CAG_nnn'CTG
Cuts at: 1486 3053 1486
Size: 1567 2614
ApaLI G'TGCA_C
Cuts at: 958 2951 958
Size: 1993 2188
ApoI r'AATT_y
Cuts at: 1042 1185 3534 3790 1042
Size: 143 2349 256 1433
Fragments arranged by size:
2349 1433 256 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 1201 1201
Size: 4181
BamHI G'GATC_C
Cuts at: 602 1206 602
Size: 604 3577
BanI G'GyrC_C
Cuts at: 1197 1611 1859 1894 1197
Size: 414 248 35 3484
Fragments arranged by size:
3484 414 248 35
BanII G_rGCy'C
Cuts at: 172 1195 2225 172
Size: 1023 1030 2128
Fragments arranged by size:
2128 1030 1023
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbsI GAAGACnn'nnnn_
Cuts at: 1148 1148
Size: 4181
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 962 1806 1899 2955 962
Size: 844 93 1056 2188
Fragments arranged by size:
2188 1056 844 93
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 1155
Size: 4181
Bpu10I CC'TnA_GC
Cuts at: 171 171
Size: 4181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1081 1357 2236 2728 3026 817
Size: 264 276 879 492 298 1972
Fragments arranged by size:
1972 879 492 298 276 264
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 898 973 1695 3732 6
Size: 892 75 722 2037 455
Fragments arranged by size:
2037 892 722 455 75
BsaHI Gr'CG_yC
Cuts at: 596 1612 1860 2562 4058 596
Size: 1016 248 702 1496 719
Fragments arranged by size:
1496 1016 719 702 248
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 169
Size: 30 4151
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 364
Size: 4181
BseYI C'CCAG_C
Cuts at: 1058 1606 2364 2941 3437 1058
Size: 548 758 577 496 1802
Fragments arranged by size:
1802 758 577 548 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 4181
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 962 1195 1973 2163 2955 962
Size: 233 778 190 792 2188
Fragments arranged by size:
2188 792 778 233 190
Bsp1286I G_dGCh'C
Cuts at: 172 962 1195 1806 1899 1973 2163 2225 2955
Size: 790 233 611 93 74 190 62 730
Cuts at: 2955 172
Size: 1398
Fragments arranged by size:
1398 790 730 611 233 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1231 1445 1747 2128 1231
Size: 214 302 381 3284
Fragments arranged by size:
3284 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2473 2527 4011 4176 50
Size: 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 291 2093 291
Size: 1802 2379
BsrFI r'CCGG_y
Cuts at: 2179 2360 2569 2179
Size: 181 209 3791
Fragments arranged by size:
3791 209 181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 409 1558 409
Size: 1149 3032
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstEII G'GTnAC_C
Cuts at: 310 310
Size: 4181
BstYI r'GATC_y
Cuts at: 1 602 1100 1206 1673 1696 2031 2277 3289
Size: 601 498 106 467 23 335 246 1012
Cuts at: 3289 3375 3387 3733 1
Size: 86 12 346 449
Fragments arranged by size:
1012 601 498 467 449 346 335 246
106 86 23 12
BtgI C'CryG_G
Cuts at: 413 413
Size: 4181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 492 1424 492
Size: 932 3249
DraI TTT'AAA
Cuts at: 1047 1180 1047
Size: 133 4048
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 365 527 1766 1940 2331 2358 3703 365
Size: 162 1239 174 391 27 1345 843
Fragments arranged by size:
1345 1239 843 391 174 162 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2389 2699 2845 3991 2389
Size: 310 146 1146 2579
Fragments arranged by size:
2579 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 1155 2006 2438 3185 3453 128
Size: 1027 851 432 747 268 856
Fragments arranged by size:
1027 856 851 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 445 445
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 828 984 1320 828
Size: 156 336 3689
Fragments arranged by size:
3689 336 156
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 1615 1863 2885 3864 1615
Size: 248 1022 979 1932
Fragments arranged by size:
1932 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 281 313 1138 1170 2023 2053 2055
Size: 32 80 32 825 32 853 30 2
Cuts at: 2055 2085 169
Size: 30 2265
Fragments arranged by size:
2265 853 825 80 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 474 1220 474
Size: 746 3435
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3036 2852
Size: 184 3997
MscI TGG'CCA
Cuts at: 529 1942 529
Size: 1413 2768
MslI CAynn'nnrTG
Cuts at: 1075 2170 2297 1075
Size: 1095 127 2959
Fragments arranged by size:
2959 1095 127
MspA1I CmG'CkG
Cuts at: 1292 1606 1966 2979 3224 1292
Size: 314 360 1013 245 2249
Fragments arranged by size:
2249 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PfoI T'CCnGG_A
Cuts at: 917 917
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PshAI GACnn'nnGTC
Cuts at: 841 841
Size: 4181
PstI C_TGCA'G
Cuts at: 1228 1913 1228
Size: 685 3496
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1606 1966 1606
Size: 360 3821
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SfcI C'TryA_G
Cuts at: 33 480 1224 1909 2902 3093 33
Size: 447 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 447 191
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1203 1203
Size: 4181
SmlI C'TyrA_G
Cuts at: 832 1096 1336 2251 2743 3005 832
Size: 264 240 915 492 262 2008
Fragments arranged by size:
2008 915 492 264 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StyI C'CwwG_G
Cuts at: 559 806 1032 1315 1669 559
Size: 247 226 283 354 3071
Fragments arranged by size:
3071 354 283 247 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 693 1764 1897 2356 2565 693
Size: 1071 133 459 209 2309
Fragments arranged by size:
2309 1071 459 209 133
TatI w'GTAC_w
Cuts at: 344 3696 344
Size: 3352 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4149
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1676
Size: 4181
XmaI C'CCGG_G
Cuts at: 1201 1201
Size: 4181
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI