Restriction map for
pF1KE0803
(Circular) (Six-base) MAPSORT of: pF1KE0803.seq Check: 150 from: 1 to: 4049
pF1KE0803 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 581 581
Size: 4049
AccI GT'mk_AC
Cuts at: 266 911 1087 266
Size: 645 176 3228
Fragments arranged by size:
3228 645 176
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 1874 2306 3053 3321 849
Size: 1025 432 747 268 1577
Fragments arranged by size:
1577 1025 747 432 268
AfeI AGC'GCT
Cuts at: 974 974
Size: 4049
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 532 532
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 474 1354 2921 474
Size: 880 1567 1602
Fragments arranged by size:
1602 1567 880
ApaI G_GGCC'C
Cuts at: 555 555
Size: 4049
ApaLI G'TGCA_C
Cuts at: 886 2819 886
Size: 1933 2116
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 381 743 1069 381
Size: 362 326 3361
Fragments arranged by size:
3361 362 326
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 154 586 1065 1479 1727 1762 154
Size: 432 479 414 248 35 2441
Fragments arranged by size:
2441 479 432 414 248 35
BanII G_rGCy'C
Cuts at: 119 209 555 614 761 1063 2093 119
Size: 90 346 59 147 302 1030 2075
Fragments arranged by size:
2075 1030 346 302 147 90 59
BbeI G_GCGC'C
Cuts at: 158 590 1483 1731 158
Size: 432 893 248 2476
Fragments arranged by size:
2476 893 432 248
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglI GCCn_nnn'nGGC
Cuts at: 954 954
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 253 580 989 1161 253
Size: 327 409 172 3141
Fragments arranged by size:
3141 409 327 172
Bme1580I G_kGCm'C
Cuts at: 555 890 933 1674 1767 2823 555
Size: 335 43 741 93 1056 1781
Fragments arranged by size:
1781 1056 741 335 93 43
BmgBI CAC'GTC
Cuts at: 300 300
Size: 4049
BmrI ACTGGGnnnn_n'
Cuts at: 591 1261 1342 1677 3821 591
Size: 670 81 335 2144 819
Fragments arranged by size:
2144 819 670 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1003 3753 1003
Size: 2750 1299
BsaAI yAC'GTr
Cuts at: 138 1402 138
Size: 1264 2785
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 155 587 1480 1728 2430 3926 155
Size: 432 893 248 702 1496 278
Fragments arranged by size:
1496 893 702 432 278 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 769 757
Size: 12 4037
BseYI C'CCAG_C
Cuts at: 225 489 613 728 975 993 1474 2232 2809
Size: 264 124 115 247 18 481 758 577
Cuts at: 2809 3305 225
Size: 496 969
Fragments arranged by size:
969 758 577 496 481 264 247 124
115 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 553 576 412
Size: 141 23 3885
Fragments arranged by size:
3885 141 23
BsiEI CG_ry'CG
Cuts at: 104 455 1591 1637 2845 104
Size: 351 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 351 46
BsiHKAI G_wGCw'C
Cuts at: 890 1063 1841 2031 2823 890
Size: 173 778 190 792 2116
Fragments arranged by size:
2116 792 778 190 173
BsmBI CGTCTCn'nnnn_
Cuts at: 974 974
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 119 209 555 614 761 890 933 1063 1674
Size: 90 346 59 147 129 43 130 611
Cuts at: 1674 1767 1841 2031 2093 2823 119
Size: 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 346 190 147 130 129
93 90 74 62 59 43
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 581 839 1099 1313 1615 1996 581
Size: 258 260 214 302 381 2634
Fragments arranged by size:
2634 381 302 260 258 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 368 2047 2228 2437 368
Size: 1679 181 209 1980
Fragments arranged by size:
1980 1679 209 181
BssSI C'ACGA_G
Cuts at: 501 2320 2678 501
Size: 1819 358 1872
Fragments arranged by size:
1872 1819 358
BstAPI GCAn_nnn'nTGC
Cuts at: 576 1426 576
Size: 850 3199
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 312 1074 1541 1564 1899 2145 3157 3243
Size: 311 762 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 762 467 449 346 335 311 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 475 888 933 1292 475
Size: 413 45 359 3232
Fragments arranged by size:
3232 413 359 45
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DraIII CAC_nnn'GTG
Cuts at: 339 683 339
Size: 344 3705
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 341 452 476 878 896 1634 1808 2199 2226
Size: 111 24 402 18 738 174 391 27
Cuts at: 2226 3571 341
Size: 1345 819
Fragments arranged by size:
1345 819 738 402 391 174 111 27
24 18
EagI C'GGCC_G
Cuts at: 452 1634 452
Size: 1182 2867
EarI CTCTTCn'nnn_
Cuts at: 490 787 793 2072 2282 490
Size: 297 6 1279 210 2257
Fragments arranged by size:
2257 1279 297 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 949 1874 2306 3053 3321 849
Size: 100 925 432 747 268 1577
Fragments arranged by size:
1577 925 747 432 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 199 199
Size: 4049
EcoO109I rG'GnC_Cy
Cuts at: 552 724 1023 1188 552
Size: 172 299 165 3413
Fragments arranged by size:
3413 299 172 165
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 158 590 976 1483 1731 2753 3732 158
Size: 432 386 507 248 1022 979 475
Fragments arranged by size:
1022 979 507 475 432 386 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 154 586 1479 1727 154
Size: 432 893 248 2476
Fragments arranged by size:
2476 893 432 248
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 343 1810 343
Size: 1467 2582
MslI CAynn'nnrTG
Cuts at: 532 2038 2165 532
Size: 1506 127 2416
Fragments arranged by size:
2416 1506 127
MspA1I CmG'CkG
Cuts at: 584 652 732 1160 1474 1834 2847 3092 584
Size: 68 80 428 314 360 1013 245 1541
Fragments arranged by size:
1541 1013 428 360 314 245 80 68
NaeI GCC'GGC
Cuts at: 370 2230 370
Size: 1860 2189
NarI GG'CG_CC
Cuts at: 155 587 1480 1728 155
Size: 432 893 248 2476
Fragments arranged by size:
2476 893 432 248
NgoMIV G'CCGG_C
Cuts at: 368 2228 368
Size: 1860 2189
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PasI CC'CwG_GG
Cuts at: 754 933 754
Size: 179 3870
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PflMI CCAn_nnn'nTGG
Cuts at: 339 339
Size: 4049
PfoI T'CCnGG_A
Cuts at: 293 823 293
Size: 530 3519
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PmlI CAC'GTG
Cuts at: 138 138
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PspOMI G'GGCC_C
Cuts at: 551 551
Size: 4049
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 267 1092 1777 2770 2961 33
Size: 234 825 685 993 191 1121
Fragments arranged by size:
1121 993 825 685 234 191
SfoI GGC'GCC
Cuts at: 156 588 1481 1729 156
Size: 432 893 248 2476
Fragments arranged by size:
2476 893 432 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 383 745 1071 383
Size: 362 326 3361
Fragments arranged by size:
3361 362 326
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 324 1183 1537 324
Size: 859 354 2836
Fragments arranged by size:
2836 859 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 1632 1765 2224 2433 610
Size: 1022 133 459 209 2226
Fragments arranged by size:
2226 1022 459 209 133
TatI w'GTAC_w
Cuts at: 3564 3564
Size: 4049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 3566 3598 122
Size: 32 3412 32 573
Fragments arranged by size:
3412 573 32 32
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 1544 291
Size: 1253 2796
XmaI C'CCGG_G
Cuts at: 381 743 1069 381
Size: 362 326 3361
Fragments arranged by size:
3361 362 326
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI