Restriction map for
pF1KE0805
(Circular) (Six-base) MAPSORT of: pF1KE0805.seq Check: 1715 from: 1 to: 4193
pF1KE0805 4193 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 903 1027 903
Size: 124 4069
AccI GT'mk_AC
Cuts at: 638 761 1231 638
Size: 123 470 3600
Fragments arranged by size:
3600 470 123
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 2018 2450 3197 3465 279
Size: 1739 432 747 268 1007
Fragments arranged by size:
1739 1007 747 432 268
AflIII A'CryG_T
Cuts at: 845 1545 2649 3626 845
Size: 700 1104 977 1412
Fragments arranged by size:
1412 1104 977 700
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 1498 3065 1498
Size: 1567 2626
ApaI G_GGCC'C
Cuts at: 987 987
Size: 4193
ApaLI G'TGCA_C
Cuts at: 841 2963 841
Size: 2122 2071
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 343 1213 343
Size: 870 3323
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 829 1209 1623 1871 1906 829
Size: 380 414 248 35 3116
Fragments arranged by size:
3116 414 380 248 35
BanII G_rGCy'C
Cuts at: 191 987 1207 2237 191
Size: 796 220 1030 2147
Fragments arranged by size:
2147 1030 796 220
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 324 324
Size: 4193
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BfrBI ATG'CAT
Cuts at: 929 929
Size: 4193
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 832 845 987 1818 1911 2967 832
Size: 13 142 831 93 1056 2058
Fragments arranged by size:
2058 1056 831 142 93 13
BmgBI CAC'GTC
Cuts at: 870 870
Size: 4193
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 205 517 151
Size: 54 312 3827
Fragments arranged by size:
3827 312 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 493 3897 493
Size: 3404 789
BsaAI yAC'GTr
Cuts at: 840 1546 840
Size: 706 3487
BsaBI GATnn'nnATC
Cuts at: 6 514 592 1707 3744 6
Size: 508 78 1115 2037 455
Fragments arranged by size:
2037 1115 508 455 78
BsaHI Gr'CG_yC
Cuts at: 737 1624 1872 2574 4070 737
Size: 887 248 702 1496 860
Fragments arranged by size:
1496 887 860 702 248
BsaWI w'CCGG_w
Cuts at: 1121 1382 1903 2581 2855 3002 3413 1121
Size: 261 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 261 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 128 158 412 442 128
Size: 30 254 30 3879
Fragments arranged by size:
3879 254 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 811 811
Size: 4193
BseYI C'CCAG_C
Cuts at: 191 980 1618 2376 2953 3449 191
Size: 789 638 758 577 496 935
Fragments arranged by size:
935 789 758 638 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 850 409
Size: 441 3752
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 845 1207 1985 2175 2967 278
Size: 567 362 778 190 792 1504
Fragments arranged by size:
1504 792 778 567 362 190
BsmI GAATG_Cn'
Cuts at: 228 228
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 191 278 832 845 987 1207 1818 1911 1985
Size: 87 554 13 142 220 611 93 74
Cuts at: 1985 2175 2237 2967 191
Size: 190 62 730 1417
Fragments arranged by size:
1417 730 611 554 220 190 142 93
87 74 62 13
BspEI T'CCGG_A
Cuts at: 1121 3413 1121
Size: 2292 1901
BspHI T'CATG_A
Cuts at: 506 683 3369 506
Size: 177 2686 1330
Fragments arranged by size:
2686 1330 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 431 903 1027 1243 1457 1759 2140 318
Size: 113 472 124 216 214 302 381 2371
Fragments arranged by size:
2371 472 381 302 216 214 124 113
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 2105 224
Size: 1881 2312
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 223 1570 223
Size: 1347 2846
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 495 960 495
Size: 465 3728
BstYI r'GATC_y
Cuts at: 1 1218 1685 1708 2043 2289 3301 3387 3399
Size: 1217 467 23 335 246 1012 86 12
Cuts at: 3399 3745 1
Size: 346 449
Fragments arranged by size:
1217 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 404 404
Size: 4193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 2143 2324 2487 615
Size: 1528 181 163 2321
Fragments arranged by size:
2321 1528 181 163
BtsI GCAGTG_nn'
Cuts at: 256 886 1049 1436 256
Size: 630 163 387 3013
Fragments arranged by size:
3013 630 387 163
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 646 1899 2757 646
Size: 1253 858 2082
Fragments arranged by size:
2082 1253 858
EaeI y'GGCC_r
Cuts at: 401 464 626 716 1778 1952 2343 2370 3715
Size: 63 162 90 1062 174 391 27 1345
Cuts at: 3715 401
Size: 879
Fragments arranged by size:
1345 1062 879 391 174 162 90 63
27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 205 279 517 2018 2450 3197 3465 151
Size: 54 74 238 1501 432 747 268 879
Fragments arranged by size:
1501 879 747 432 268 238 74 54
EcoO109I rG'GnC_Cy
Cuts at: 699 984 1332 699
Size: 285 348 3560
Fragments arranged by size:
3560 348 285
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 1138 1974 1138
Size: 836 3357
HaeII r_GCGC'y
Cuts at: 317 1627 1875 2897 3876 317
Size: 1310 248 1022 979 634
Fragments arranged by size:
1310 1022 979 634 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 2035 2065 2067 2097 128
Size: 32 1875 30 2 30 2224
Fragments arranged by size:
2224 1875 32 30 30 2
HincII GTy'rAC
Cuts at: 639 1232 639
Size: 593 3600
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3048 2864
Size: 184 4009
MscI TGG'CCA
Cuts at: 628 718 1954 628
Size: 90 1236 2867
Fragments arranged by size:
2867 1236 90
MslI CAynn'nnrTG
Cuts at: 688 799 2182 2309 688
Size: 111 1383 127 2572
Fragments arranged by size:
2572 1383 127 111
MspA1I CmG'CkG
Cuts at: 1304 1618 1978 2991 3236 1304
Size: 314 360 1013 245 2261
Fragments arranged by size:
2261 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NsiI A_TGCA'T
Cuts at: 931 931
Size: 4193
NspI r_CATG'y
Cuts at: 474 849 1246 1746 2653 474
Size: 375 397 500 907 2014
Fragments arranged by size:
2014 907 500 397 375
PciI A'CATG_T
Cuts at: 845 2649 845
Size: 1804 2389
PflMI CCAn_nnn'nTGG
Cuts at: 564 564
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PspOMI G'GGCC_C
Cuts at: 983 983
Size: 4193
PstI C_TGCA'G
Cuts at: 454 1240 1925 454
Size: 786 685 2722
Fragments arranged by size:
2722 786 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1618 1978 1618
Size: 360 3833
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 637 1230 637
Size: 593 3600
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SexAI A'CCwGG_T
Cuts at: 1001 1070 1001
Size: 69 4124
SfcI C'TryA_G
Cuts at: 33 450 1236 1921 2914 3105 33
Size: 417 786 685 993 191 1121
Fragments arranged by size:
1121 993 786 685 417 191
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1348 2263 2755 3017 1348
Size: 915 492 262 2524
Fragments arranged by size:
2524 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 1327 1681 1327
Size: 354 3839
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 437 3708 437
Size: 3271 922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI