Restriction map for
pF1KE0807
(Circular) (Six-base) MAPSORT of: pF1KE0807.seq Check: 3980 from: 1 to: 4196
pF1KE0807 4196 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 263 1234 263
Size: 971 3225
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2453 3200 3468 2021
Size: 432 747 268 2749
Fragments arranged by size:
2749 747 432 268
AfeI AGC'GCT
Cuts at: 352 352
Size: 4196
AflIII A'CryG_T
Cuts at: 1548 2652 3629 1548
Size: 1104 977 2115
Fragments arranged by size:
2115 1104 977
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 499 538 541 559 499
Size: 39 3 18 4136
Fragments arranged by size:
4136 39 18 3
AlwNI CAG_nnn'CTG
Cuts at: 654 1122 1501 3068 654
Size: 468 379 1567 1782
Fragments arranged by size:
1782 1567 468 379
ApaI G_GGCC'C
Cuts at: 636 636
Size: 4196
ApaLI G'TGCA_C
Cuts at: 484 544 2966 484
Size: 60 2422 1714
Fragments arranged by size:
2422 1714 60
ApoI r'AATT_y
Cuts at: 729 1200 3549 3805 729
Size: 471 2349 256 1120
Fragments arranged by size:
2349 1120 471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 185 873 1216 185
Size: 688 343 3165
Fragments arranged by size:
3165 688 343
AvrII C'CTAG_G
Cuts at: 297 555 297
Size: 258 3938
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 616 1212 1626 1874 1909 616
Size: 596 414 248 35 2903
Fragments arranged by size:
2903 596 414 248 35
BanII G_rGCy'C
Cuts at: 165 636 1210 2240 165
Size: 471 574 1030 2121
Fragments arranged by size:
2121 1030 574 471
BbeI G_GCGC'C
Cuts at: 1630 1878 1630
Size: 248 3948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4162
BciVI GTATCCnnnnn_n'
Cuts at: 2094 2855 4196 2094
Size: 761 1341 2094
Fragments arranged by size:
2094 1341 761
BclI T'GATC_A
Cuts at: 831 1716 831
Size: 885 3311
BglI GCCn_nnn'nGGC
Cuts at: 1016 1016
Size: 4196
BglII A'GATC_T
Cuts at: 1183 1711 3748 1183
Size: 528 2037 1631
Fragments arranged by size:
2037 1631 528
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 488 548 619 636 936 1821 1914 2970 488
Size: 60 71 17 300 885 93 1056 1714
Fragments arranged by size:
1714 1056 885 300 93 71 60 17
BmrI ACTGGGnnnn_n'
Cuts at: 1061 1408 1489 1824 3968 1061
Size: 347 81 335 2144 1289
Fragments arranged by size:
2144 1289 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 198 1126 2563 184
Size: 14 928 1437 1817
Fragments arranged by size:
1817 1437 928 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 375 631 1099 193
Size: 182 256 468 3290
Fragments arranged by size:
3290 468 256 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2251 2743 3041 1372
Size: 879 492 298 2527
Fragments arranged by size:
2527 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 235 3900 235
Size: 3665 531
BsaAI yAC'GTr
Cuts at: 1549 1549
Size: 4196
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 1627 1875 2577 4073 1627
Size: 248 702 1496 1750
Fragments arranged by size:
1750 1496 702 248
BsaWI w'CCGG_w
Cuts at: 590 888 1385 1906 2584 2858 3005 3416 590
Size: 298 497 521 678 274 147 411 1370
Fragments arranged by size:
1370 678 521 497 411 298 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 141 171 141
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 857 1102 857
Size: 245 3951
BseYI C'CCAG_C
Cuts at: 612 1153 1621 2379 2956 3452 612
Size: 541 468 758 577 496 1356
Fragments arranged by size:
1356 758 577 541 496 468
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 165 488 548 1210 1988 2178 2970 165
Size: 323 60 662 778 190 792 1391
Fragments arranged by size:
1391 792 778 662 323 190 60
Bsp1286I G_dGCh'C
Cuts at: 165 488 548 619 636 936 1210 1821 1914
Size: 323 60 71 17 300 274 611 93
Cuts at: 1914 1988 2178 2240 2970 165
Size: 74 190 62 730 1391
Fragments arranged by size:
1391 730 611 323 300 274 190 93
74 71 62 60 17
BspEI T'CCGG_A
Cuts at: 888 3416 888
Size: 2528 1668
BspHI T'CATG_A
Cuts at: 852 3372 852
Size: 2520 1676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 407 541 1246 1460 1762 2143 407
Size: 134 705 214 302 381 2460
Fragments arranged by size:
2460 705 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 2488 2542 4026 4191 50
Size: 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2108 2108
Size: 4196
BsrFI r'CCGG_y
Cuts at: 2194 2375 2584 2194
Size: 181 209 3806
Fragments arranged by size:
3806 209 181
BssSI C'ACGA_G
Cuts at: 2467 2825 2467
Size: 358 3838
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 1573
Size: 4196
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 375 901 375
Size: 526 3670
BstYI r'GATC_y
Cuts at: 1 1183 1221 1688 1711 2046 2292 3304 3390
Size: 1182 38 467 23 335 246 1012 86
Cuts at: 3390 3402 3748 1
Size: 12 346 449
Fragments arranged by size:
1182 1012 467 449 346 335 246 86
38 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 392 693 1781 1955 2346 2373 3718 392
Size: 301 1088 174 391 27 1345 870
Fragments arranged by size:
1345 1088 870 391 301 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 920 1156 2219 2429 920
Size: 236 1063 210 2687
Fragments arranged by size:
2687 1063 236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 2714 2860 4006 2404
Size: 310 146 1146 2594
Fragments arranged by size:
2594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 163 1208 163
Size: 1045 3151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 375 631 1099 2021 2453 3200 3468 193
Size: 182 256 468 922 432 747 268 921
Fragments arranged by size:
922 921 747 468 432 268 256 182
EcoO109I rG'GnC_Cy
Cuts at: 633 1335 633
Size: 702 3494
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 354 1630 1878 2900 3879 354
Size: 1276 248 1022 979 671
Fragments arranged by size:
1276 1022 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2038 2068 2070 2100 2038
Size: 30 2 30 4134
Fragments arranged by size:
4134 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
KasI G'GCGC_C
Cuts at: 1626 1874 1626
Size: 248 3948
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2867 3051 2867
Size: 184 4012
MscI TGG'CCA
Cuts at: 695 1957 695
Size: 1262 2934
MslI CAynn'nnrTG
Cuts at: 541 800 2185 2312 541
Size: 259 1385 127 2425
Fragments arranged by size:
2425 1385 259 127
MspA1I CmG'CkG
Cuts at: 651 1056 1153 1307 1621 1981 2994 3239 651
Size: 405 97 154 314 360 1013 245 1608
Fragments arranged by size:
1608 1013 405 360 314 245 154 97
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1627 1875 1627
Size: 248 3948
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NspI r_CATG'y
Cuts at: 607 1249 1749 2656 607
Size: 642 500 907 2147
Fragments arranged by size:
2147 907 642 500
PasI CC'CwG_GG
Cuts at: 1171 1171
Size: 4196
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PflMI CCAn_nnn'nTGG
Cuts at: 313 353 313
Size: 40 4156
PfoI T'CCnGG_A
Cuts at: 170 170
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 215 247 3335 3367 215
Size: 32 3088 32 1044
Fragments arranged by size:
3088 1044 32 32
PspOMI G'GGCC_C
Cuts at: 632 632
Size: 4196
PstI C_TGCA'G
Cuts at: 1243 1928 1243
Size: 685 3511
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 651 1056 1153 1621 1981 651
Size: 405 97 468 360 2866
Fragments arranged by size:
2866 468 405 360 97
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 165 1210 165
Size: 1045 3151
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 279 1239 1924 2917 3108 33
Size: 246 960 685 993 191 1121
Fragments arranged by size:
1121 993 960 685 246 191
SfoI GGC'GCC
Cuts at: 1628 1876 1628
Size: 248 3948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 187 1218 187
Size: 1031 3165
SmlI C'TyrA_G
Cuts at: 1351 2266 2758 3020 1351
Size: 915 492 262 2527
Fragments arranged by size:
2527 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 607 1249 1749 607
Size: 642 500 3054
Fragments arranged by size:
3054 642 500
StuI AGG'CCT
Cuts at: 1036 1036
Size: 4196
StyI C'CwwG_G
Cuts at: 297 555 789 1330 1684 297
Size: 258 234 541 354 2809
Fragments arranged by size:
2809 541 354 258 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 584 1129 1779 1912 2371 2580 584
Size: 545 650 133 459 209 2200
Fragments arranged by size:
2200 650 545 459 209 133
TatI w'GTAC_w
Cuts at: 251 1074 3711 251
Size: 823 2637 736
Fragments arranged by size:
2637 823 736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 141 173 3713 3745 141
Size: 32 3540 32 592
Fragments arranged by size:
3540 592 32 32
Tth111I GACn'n_nGTC
Cuts at: 768 1993 768
Size: 1225 2971
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 633 1691 372
Size: 261 1058 2877
Fragments arranged by size:
2877 1058 261
XmaI C'CCGG_G
Cuts at: 185 1216 185
Size: 1031 3165
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI BaeI
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI