Restriction map for
pF1KE0809
(Circular) (Six-base) MAPSORT of: pF1KE0809.seq Check: 7850 from: 1 to: 4118
pF1KE0809 4118 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 840 840
Size: 4118
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 416 893 1134 416
Size: 477 241 3400
Fragments arranged by size:
3400 477 241
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1943 2375 3122 3390 1943
Size: 432 747 268 2671
Fragments arranged by size:
2671 747 432 268
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AleI CACnn'nnGTG
Cuts at: 178 178
Size: 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 940
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 230 1423 2990 230
Size: 1193 1567 1358
Fragments arranged by size:
1567 1358 1193
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
AvrII C'CTAG_G
Cuts at: 990 990
Size: 4118
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 416 893 1134 1548 1796 1831 416
Size: 477 241 414 248 35 2703
Fragments arranged by size:
2703 477 414 248 241 35
BanII G_rGCy'C
Cuts at: 1132 2162 1132
Size: 1030 3088
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbsI GAAGACnn'nnnn_
Cuts at: 337 645 337
Size: 308 3810
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 401 1638 401
Size: 1237 2881
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 1743 1836 2892 1743
Size: 93 1056 2969
Fragments arranged by size:
2969 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 827 2485 827
Size: 1658 2460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 755 1077 291
Size: 464 322 3332
Fragments arranged by size:
3332 464 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1294 2173 2665 2963 658
Size: 636 879 492 298 1813
Fragments arranged by size:
1813 879 636 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 4088
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 634 1012 634
Size: 378 3740
BseYI C'CCAG_C
Cuts at: 633 760 1543 2301 2878 3374 633
Size: 127 783 758 577 496 1377
Fragments arranged by size:
1377 783 758 577 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 591 868 360
Size: 231 277 3610
Fragments arranged by size:
3610 277 231
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1010 1132 1910 2100 2892 1010
Size: 122 778 190 792 2236
Fragments arranged by size:
2236 792 778 190 122
Bsp1286I G_dGCh'C
Cuts at: 1010 1132 1743 1836 1910 2100 2162 2892 1010
Size: 122 611 93 74 190 62 730 2236
Fragments arranged by size:
2236 730 611 190 122 93 74 62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 455 3294 455
Size: 2839 1279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 840 1168 1382 1684 2065 840
Size: 328 214 302 381 2893
Fragments arranged by size:
2893 381 328 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 482 2030 161
Size: 321 1548 2249
Fragments arranged by size:
2249 1548 321
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 705 2479 705
Size: 1774 2344
BstYI r'GATC_y
Cuts at: 1 1143 1610 1633 1968 2214 3226 3312 3324
Size: 1142 467 23 335 246 1012 86 12
Cuts at: 3324 3670 1
Size: 346 449
Fragments arranged by size:
1142 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 728 751 107
Size: 621 23 3474
Fragments arranged by size:
3474 621 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 574 1361 574
Size: 787 3331
ClaI AT'CG_AT
Cuts at: 744 744
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DraIII CAC_nnn'GTG
Cuts at: 603 603
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 275 470 1034 1703 1877 2268 2295 3640 275
Size: 195 564 669 174 391 27 1345 753
Fragments arranged by size:
1345 753 669 564 391 195 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 755 1077 1943 2375 3122 3390 291
Size: 464 322 866 432 747 268 1019
Fragments arranged by size:
1019 866 747 464 432 322 268
EcoNI CCTnn'n_nnAGG
Cuts at: 411 411
Size: 4118
EcoO109I rG'GnC_Cy
Cuts at: 476 1257 476
Size: 781 3337
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 453 2137 453
Size: 1684 2434
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 1552 1800 2822 3801 1552
Size: 248 1022 979 1869
Fragments arranged by size:
1869 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 959 991 1960 1990 1992 2022 289
Size: 32 638 32 969 30 2 30 2385
Fragments arranged by size:
2385 969 638 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 420 897 1138 420
Size: 477 241 3400
Fragments arranged by size:
3400 477 241
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 277 1879 277
Size: 1602 2516
MslI CAynn'nnrTG
Cuts at: 178 212 394 2107 2234 178
Size: 34 182 1713 127 2062
Fragments arranged by size:
2062 1713 182 127 34
MspA1I CmG'CkG
Cuts at: 376 1229 1543 1903 2916 3161 376
Size: 853 314 360 1013 245 1333
Fragments arranged by size:
1333 1013 853 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 728 107
Size: 621 3497
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 672 721 1171 1671 2578 672
Size: 49 450 500 907 2212
Fragments arranged by size:
2212 907 500 450 49
PasI CC'CwG_GG
Cuts at: 1061 1061
Size: 4118
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PfoI T'CCnGG_A
Cuts at: 817 817
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PstI C_TGCA'G
Cuts at: 974 1165 1850 974
Size: 191 685 3242
Fragments arranged by size:
3242 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 376 1543 1903 376
Size: 1167 360 2591
Fragments arranged by size:
2591 1167 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 970 1161 1846 2839 3030 33
Size: 937 191 685 993 191 1121
Fragments arranged by size:
1121 993 937 685 191 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 673 1273 2188 2680 2942 673
Size: 600 915 492 262 1849
Fragments arranged by size:
1849 915 600 492 262
SpeI A'CTAG_T
Cuts at: 67 177 67
Size: 110 4008
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StyI C'CwwG_G
Cuts at: 107 728 990 1252 1606 107
Size: 621 262 262 354 2619
Fragments arranged by size:
2619 621 354 262 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1701 1834 2293 2502 1701
Size: 133 459 209 3317
Fragments arranged by size:
3317 459 209 133
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 518 550 3635 3667 518
Size: 32 3085 32 969
Fragments arranged by size:
3085 969 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 1613 214
Size: 1399 2719
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI