Restriction map for
pF1KE0821
(Circular) (Six-base) MAPSORT of: pF1KE0821.seq Check: 9933 from: 1 to: 3923
pF1KE0821 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1748 2180 2927 3195 649
Size: 1099 432 747 268 1377
Fragments arranged by size:
1377 1099 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AleI CACnn'nnGTG
Cuts at: 370 370
Size: 3923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 299
Size: 32 3891
AlwNI CAG_nnn'CTG
Cuts at: 1228 2795 1228
Size: 1567 2356
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 943 943
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 285 937 1967 285
Size: 652 1030 2241
Fragments arranged by size:
2241 1030 652
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbsI GAAGACnn'nnnn_
Cuts at: 112 315 112
Size: 203 3720
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 466 700 466
Size: 234 3689
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 1548 1641 2697 1548
Size: 93 1056 2774
Fragments arranged by size:
2774 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 900 2290 900
Size: 1390 2533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 790 232
Size: 558 3365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 1099 1978 2470 2768 549
Size: 550 879 492 298 1704
Fragments arranged by size:
1704 879 550 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 299
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 876 876
Size: 3923
BseYI C'CCAG_C
Cuts at: 406 839 1348 2106 2683 3179 406
Size: 433 509 758 577 496 1150
Fragments arranged by size:
1150 758 577 509 496 433
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 683 937 1715 1905 2697 683
Size: 254 778 190 792 1909
Fragments arranged by size:
1909 792 778 254 190
BsmI GAATG_Cn'
Cuts at: 775 775
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 285 683 937 1548 1641 1715 1905 1967 2697
Size: 398 254 611 93 74 190 62 730
Cuts at: 2697 285
Size: 1511
Fragments arranged by size:
1511 730 611 398 254 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BsrGI T'GTAC_A
Cuts at: 254 254
Size: 3923
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 377 377
Size: 3923
BtgI C'CryG_G
Cuts at: 491 491
Size: 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 260 1166 260
Size: 906 3017
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 718 1629 2487 718
Size: 911 858 2154
Fragments arranged by size:
2154 911 858
EaeI y'GGCC_r
Cuts at: 488 1508 1682 2073 2100 3445 488
Size: 1020 174 391 27 1345 966
Fragments arranged by size:
1345 1020 966 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 625 894 1946 2156 625
Size: 269 1052 210 2392
Fragments arranged by size:
2392 1052 269 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 649 790 1748 2180 2927 3195 232
Size: 417 141 958 432 747 268 960
Fragments arranged by size:
960 958 747 432 417 268 141
EcoO109I rG'GnC_Cy
Cuts at: 1062 1062
Size: 3923
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
EcoRV GAT'ATC
Cuts at: 351 351
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 272 304 1765 1795 1797 1827 272
Size: 32 1461 30 2 30 2368
Fragments arranged by size:
2368 1461 32 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MfeI C'AATT_G
Cuts at: 697 697
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 490 1684 490
Size: 1194 2729
MslI CAynn'nnrTG
Cuts at: 370 688 1912 2039 370
Size: 318 1224 127 2254
Fragments arranged by size:
2254 1224 318 127
MspA1I CmG'CkG
Cuts at: 1034 1348 1708 2721 2966 1034
Size: 314 360 1013 245 1991
Fragments arranged by size:
1991 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 491 491
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 150 976 1476 2383 150
Size: 826 500 907 1690
Fragments arranged by size:
1690 907 826 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 299 331 3062 3094 299
Size: 32 2731 32 1128
Fragments arranged by size:
2731 1128 32 32
PstI C_TGCA'G
Cuts at: 267 970 1655 267
Size: 703 685 2535
Fragments arranged by size:
2535 703 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 1348 1708 1348
Size: 360 3563
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 263 966 1651 2644 2835 33
Size: 230 703 685 993 191 1121
Fragments arranged by size:
1121 993 703 685 230 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 700 700
Size: 3923
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 528 1078 1993 2485 2747 528
Size: 550 915 492 262 1704
Fragments arranged by size:
1704 915 550 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StuI AGG'CCT
Cuts at: 382 382
Size: 3923
StyI C'CwwG_G
Cuts at: 195 383 491 1057 1411 195
Size: 188 108 566 354 2707
Fragments arranged by size:
2707 566 354 188 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 254 520 3438 254
Size: 266 2918 739
Fragments arranged by size:
2918 739 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 159 191 3440 3472 159
Size: 32 3249 32 610
Fragments arranged by size:
3249 610 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI