Restriction map for
pF1KE0828
(Circular) (Six-base) MAPSORT of: pF1KE0828.seq Check: 2400 from: 1 to: 3683
pF1KE0828 3683 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 988 2555 988
Size: 1567 2116
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 640 687 3036 3292 640
Size: 47 2349 256 1031
Fragments arranged by size:
2349 1031 256 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 703 703
Size: 3683
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 124 157 124
Size: 33 3650
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 211 699 1113 1361 1396 211
Size: 488 414 248 35 2498
Fragments arranged by size:
2498 488 414 248 35
BanII G_rGCy'C
Cuts at: 697 1727 697
Size: 1030 2653
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BciVI GTATCCnnnnn_n'
Cuts at: 1581 2342 3683 1581
Size: 761 1341 1581
Fragments arranged by size:
1581 1341 761
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BfrBI ATG'CAT
Cuts at: 233 233
Size: 3683
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 377 1308 1401 2457 377
Size: 931 93 1056 1603
Fragments arranged by size:
1603 1056 931 93
BmrI ACTGGGnnnn_n'
Cuts at: 895 976 1311 3455 895
Size: 81 335 2144 1123
Fragments arranged by size:
2144 1123 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 286 399 156
Size: 130 113 3440
Fragments arranged by size:
3440 130 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3387 3387
Size: 3683
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 872 1393 2071 2345 2492 2903 872
Size: 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 433
Size: 30 3653
BseYI C'CCAG_C
Cuts at: 541 1108 1866 2443 2939 541
Size: 567 758 577 496 1285
Fragments arranged by size:
1285 758 577 567 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 297
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 172 697 1475 1665 2457 172
Size: 525 778 190 792 1398
Fragments arranged by size:
1398 792 778 525 190
Bsp1286I G_dGCh'C
Cuts at: 172 377 697 1308 1401 1475 1665 1727 2457
Size: 205 320 611 93 74 190 62 730
Cuts at: 2457 172
Size: 1398
Fragments arranged by size:
1398 730 611 320 205 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 733 947 1249 1630 437
Size: 296 214 302 381 2490
Fragments arranged by size:
2490 381 302 296 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 146 1595 146
Size: 1449 2234
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstYI r'GATC_y
Cuts at: 1 708 1175 1198 1533 1779 2791 2877 2889
Size: 707 467 23 335 246 1012 86 12
Cuts at: 2889 3235 1
Size: 346 449
Fragments arranged by size:
1012 707 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 622 622
Size: 3683
BtgI C'CryG_G
Cuts at: 215 548 215
Size: 333 3350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 926 926
Size: 3683
ClaI AT'CG_AT
Cuts at: 269 269
Size: 3683
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 119 1268 1442 1833 1860 3205 119
Size: 1149 174 391 27 1345 597
Fragments arranged by size:
1345 1149 597 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 286 399 1508 1940 2687 2955 156
Size: 130 113 1109 432 747 268 884
Fragments arranged by size:
1109 884 747 432 268 130 113
EcoO109I rG'GnC_Cy
Cuts at: 822 822
Size: 3683
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1702
Size: 3683
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 1117 1365 2387 3366 1117
Size: 248 1022 979 1434
Fragments arranged by size:
1434 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 1525 1555 1557 1587 433
Size: 32 1060 30 2 30 2529
Fragments arranged by size:
2529 1060 32 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
HindIII A'AGCT_T
Cuts at: 455 455
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2538 2354
Size: 184 3499
MscI TGG'CCA
Cuts at: 121 1444 121
Size: 1323 2360
MslI CAynn'nnrTG
Cuts at: 151 370 1672 1799 151
Size: 219 1302 127 2035
Fragments arranged by size:
2035 1302 219 127
MspA1I CmG'CkG
Cuts at: 794 1108 1468 2481 2726 794
Size: 314 360 1013 245 1751
Fragments arranged by size:
1751 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 215 548 215
Size: 333 3350
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NsiI A_TGCA'T
Cuts at: 235 235
Size: 3683
NspI r_CATG'y
Cuts at: 237 736 1236 2143 237
Size: 499 500 907 1777
Fragments arranged by size:
1777 907 500 499
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PstI C_TGCA'G
Cuts at: 730 1415 730
Size: 685 2998
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 1108 1468 1108
Size: 360 3323
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 730 730
Size: 3683
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 161 615 726 1411 2404 2595 33
Size: 128 454 111 685 993 191 1121
Fragments arranged by size:
1121 993 685 454 191 128 111
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 838 1753 2245 2507 838
Size: 915 492 262 2014
Fragments arranged by size:
2014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 237 736 1236 237
Size: 499 500 2684
Fragments arranged by size:
2684 500 499
StyI C'CwwG_G
Cuts at: 215 437 548 817 1171 215
Size: 222 111 269 354 2727
Fragments arranged by size:
2727 354 269 222 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 3198 3198
Size: 3683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1178 114
Size: 1064 2619
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI