Restriction map for
pF1KE0834
(Circular) (Six-base) MAPSORT of: pF1KE0834.seq Check: 8076 from: 1 to: 3920
pF1KE0834 3920 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 196 196
Size: 3920
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 733 936 733
Size: 203 3717
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 1745 2177 2924 3192 585
Size: 1160 432 747 268 1313
Fragments arranged by size:
1313 1160 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AleI CACnn'nnGTG
Cuts at: 814 814
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 1225 2792 1225
Size: 1567 2353
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 656 945 656
Size: 289 3631
BanI G'GyrC_C
Cuts at: 733 936 1350 1598 1633 733
Size: 203 414 248 35 3020
Fragments arranged by size:
3020 414 248 203 35
BanII G_rGCy'C
Cuts at: 211 934 1964 211
Size: 723 1030 2167
Fragments arranged by size:
2167 1030 723
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 591 591
Size: 3920
BbvCI CC'TCA_GC
Cuts at: 163 163
Size: 3920
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BfrBI ATG'CAT
Cuts at: 463 463
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 1545 1638 2694 1545
Size: 93 1056 2771
Fragments arranged by size:
2771 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 755 1132 1213 1548 3692 755
Size: 377 81 335 2144 983
Fragments arranged by size:
2144 983 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 605
Size: 3920
Bpu10I CC'TnA_GC
Cuts at: 163 163
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1096 1975 2467 2765 870
Size: 226 879 492 298 2025
Fragments arranged by size:
2025 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 186
Size: 3920
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 211 934 1545 1638 1712 1902 1964 2694 211
Size: 723 611 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 723 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 434 3096 434
Size: 2662 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 196 970 1184 1486 1867 196
Size: 774 214 302 381 2249
Fragments arranged by size:
2249 774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 662 2212 2266 3750 3915 50
Size: 612 1550 54 1484 165 55
Fragments arranged by size:
1550 1484 612 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BsrGI T'GTAC_A
Cuts at: 753 753
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 656 780 945 1412 1435 1770 2016 3028
Size: 655 124 165 467 23 335 246 1012
Cuts at: 3028 3114 3126 3472 1
Size: 86 12 346 449
Fragments arranged by size:
1012 655 467 449 346 335 246 165
124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 652 652
Size: 3920
BtgI C'CryG_G
Cuts at: 326 326
Size: 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 283 821 1163 283
Size: 538 342 3040
Fragments arranged by size:
3040 538 342
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 347 644 1505 1679 2070 2097 3442 347
Size: 297 861 174 391 27 1345 825
Fragments arranged by size:
1345 861 825 391 297 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 232 705 1943 2153 232
Size: 473 1238 210 1999
Fragments arranged by size:
1999 1238 473 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 605 1745 2177 2924 3192 585
Size: 20 1140 432 747 268 1313
Fragments arranged by size:
1313 1140 747 432 268 20
EcoO109I rG'GnC_Cy
Cuts at: 415 1059 415
Size: 644 3276
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 876 908 1762 1792 1794 1824 876
Size: 32 854 30 2 30 2972
Fragments arranged by size:
2972 854 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 737 940 737
Size: 203 3717
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 349 646 1681 349
Size: 297 1035 2588
Fragments arranged by size:
2588 1035 297
MslI CAynn'nnrTG
Cuts at: 148 640 761 814 1909 2036 148
Size: 492 121 53 1095 127 2032
Fragments arranged by size:
2032 1095 492 127 121 53
MspA1I CmG'CkG
Cuts at: 715 1031 1345 1705 2718 2963 715
Size: 316 314 360 1013 245 1672
Fragments arranged by size:
1672 1013 360 316 314 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 326 326
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NsiI A_TGCA'T
Cuts at: 465 465
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PflMI CCAn_nnn'nTGG
Cuts at: 408 408
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 415 415
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 715 1345 1705 715
Size: 630 360 2930
Fragments arranged by size:
2930 630 360
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SanDI GG'GwC_CC
Cuts at: 415 415
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 705 1943 2153 705
Size: 1238 210 2472
Fragments arranged by size:
2472 1238 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 843 963 1648 2641 2832 33
Size: 810 120 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191 120
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 849 1075 1990 2482 2744 849
Size: 226 915 492 262 2025
Fragments arranged by size:
2025 915 492 262 226
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 258 326 1054 1408 258
Size: 68 728 354 2770
Fragments arranged by size:
2770 728 354 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 162 1503 1636 2095 2304 162
Size: 1341 133 459 209 1778
Fragments arranged by size:
1778 1341 459 209 133
TatI w'GTAC_w
Cuts at: 152 753 3435 152
Size: 601 2682 637
Fragments arranged by size:
2682 637 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
XmnI GAAnn'nnTTC
Cuts at: 129 129
Size: 3920
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI