Restriction map for
pF1KE0839
(Circular) (Six-base) MAPSORT of: pF1KE0839.seq Check: 4647 from: 1 to: 4049
pF1KE0839 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1009 1874 2306 3053 3321 493
Size: 516 865 432 747 268 1221
Fragments arranged by size:
1221 865 747 516 432 268
AfeI AGC'GCT
Cuts at: 870 870
Size: 4049
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 275 1354 2921 275
Size: 1079 1567 1403
Fragments arranged by size:
1567 1403 1079
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 610 626 1053 3402 3658 610
Size: 16 427 2349 256 1001
Fragments arranged by size:
2349 1001 427 256 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4015
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 251 809 1569 251
Size: 558 760 2731
Fragments arranged by size:
2731 760 558
BglI GCCn_nnn'nGGC
Cuts at: 802 802
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 767 838 1261 1342 1677 3821 767
Size: 71 423 81 335 2144 995
Fragments arranged by size:
2144 995 423 335 81 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 252
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1225 2104 2596 2894 418
Size: 807 879 492 298 1573
Fragments arranged by size:
1573 879 807 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 761 3753 761
Size: 2992 1057
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 241 1563 3600 6
Size: 235 1322 2037 455
Fragments arranged by size:
2037 1322 455 235
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 817 814
Size: 3 4046
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1063 1674 1767 1841 2031 2093 2823 1063
Size: 611 93 74 190 62 730 2289
Fragments arranged by size:
2289 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 588 3225 588
Size: 2637 1412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 881 881
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 682 1999 2180 2343 682
Size: 1317 181 163 2388
Fragments arranged by size:
2388 1317 181 163
BtsI GCAGTG_nn'
Cuts at: 812 1292 812
Size: 480 3569
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 260 1634 1808 2199 2226 3571 260
Size: 1374 174 391 27 1345 738
Fragments arranged by size:
1374 1345 738 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 216 2072 2282 216
Size: 1856 210 1983
Fragments arranged by size:
1983 1856 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 493 1009 1874 2306 3053 3321 252
Size: 241 516 865 432 747 268 980
Fragments arranged by size:
980 865 747 516 432 268 241
EcoO109I rG'GnC_Cy
Cuts at: 515 1188 515
Size: 673 3376
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1049 2068 1049
Size: 1019 3030
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 872 1483 1731 2753 3732 872
Size: 611 248 1022 979 1189
Fragments arranged by size:
1189 1022 979 611 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 198 650 1088 198
Size: 452 438 3159
Fragments arranged by size:
3159 452 438
HindIII A'AGCT_T
Cuts at: 980 980
Size: 4049
HpaI GTT'AAC
Cuts at: 198 650 198
Size: 452 3597
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 262 1810 262
Size: 1548 2501
MslI CAynn'nnrTG
Cuts at: 2038 2165 2038
Size: 127 3922
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NdeI CA'TA_TG
Cuts at: 333 333
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 754 1102 1602 2509 754
Size: 348 500 907 2294
Fragments arranged by size:
2294 907 500 348
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PpuMI rG'GwC_Cy
Cuts at: 515 515
Size: 4049
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 755 1092 1777 2770 2961 33
Size: 722 337 685 993 191 1121
Fragments arranged by size:
1121 993 722 685 337 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 433 1204 2119 2611 2873 433
Size: 771 915 492 262 1609
Fragments arranged by size:
1609 915 771 492 262
SpeI A'CTAG_T
Cuts at: 67 192 67
Size: 125 3924
SphI G_CATG'C
Cuts at: 754 1102 1602 754
Size: 348 500 3201
Fragments arranged by size:
3201 500 348
StyI C'CwwG_G
Cuts at: 1183 1537 1183
Size: 354 3695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 1632 1765 2224 2433 470
Size: 1162 133 459 209 2086
Fragments arranged by size:
2086 1162 459 209 133
TatI w'GTAC_w
Cuts at: 189 775 836 3564 189
Size: 586 61 2728 674
Fragments arranged by size:
2728 674 586 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 3566 3598 878
Size: 32 2656 32 1329
Fragments arranged by size:
2656 1329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 464 1544 464
Size: 1080 2969
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 983 1012 983
Size: 29 4020
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI