Restriction map for
pF1KE0841
(Circular) (Six-base) MAPSORT of: pF1KE0841.seq Check: 782 from: 1 to: 4139
pF1KE0841 4139 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 925 1177 925
Size: 252 3887
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 434 1444 3011 434
Size: 1010 1567 1562
Fragments arranged by size:
1567 1562 1010
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 412 554 986 1143 3492 3748 412
Size: 142 432 157 2349 256 803
Fragments arranged by size:
2349 803 432 256 157 142
AseI AT'TA_AT
Cuts at: 20 940 20
Size: 920 3219
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 197 1159 197
Size: 962 3177
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 1153 2183 1153
Size: 1030 3109
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 325 325
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 1764 1857 2913 1764
Size: 93 1056 2990
Fragments arranged by size:
2990 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 590 1492 590
Size: 902 3237
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 695 1564 2322 2899 3395 695
Size: 869 758 577 496 1439
Fragments arranged by size:
1439 869 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 277 157
Size: 120 4019
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 654 1153 1931 2121 2913 654
Size: 499 778 190 792 1880
Fragments arranged by size:
1880 792 778 499 190
Bsp1286I G_dGCh'C
Cuts at: 654 1153 1764 1857 1931 2121 2183 2913 654
Size: 499 611 93 74 190 62 730 1880
Fragments arranged by size:
1880 730 611 499 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 314 2051 314
Size: 1737 2402
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BsrGI T'GTAC_A
Cuts at: 209 209
Size: 4139
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 917 917
Size: 4139
BstYI r'GATC_y
Cuts at: 1 163 1164 1631 1654 1989 2235 3247 3333
Size: 162 1001 467 23 335 246 1012 86
Cuts at: 3333 3345 3691 1
Size: 12 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 246 162
86 23 12
BstZ17I GTA'TAC
Cuts at: 926 926
Size: 4139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1097 1138 1097
Size: 41 4098
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 851 1724 1898 2289 2316 3661 851
Size: 873 174 391 27 1345 1329
Fragments arranged by size:
1345 1329 873 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 428 462 2162 2372 428
Size: 34 1700 210 2195
Fragments arranged by size:
2195 1700 210 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1964 2396 3143 3411 655
Size: 1309 432 747 268 1383
Fragments arranged by size:
1383 1309 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 194 1278 194
Size: 1084 3055
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 567 599 1023 1055 1981 2011 2013 2043 567
Size: 32 424 32 926 30 2 30 2663
Fragments arranged by size:
2663 926 424 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 325 976 2810 2994 325
Size: 651 1834 184 1470
Fragments arranged by size:
1834 1470 651 184
MscI TGG'CCA
Cuts at: 853 1900 853
Size: 1047 3092
MslI CAynn'nnrTG
Cuts at: 565 2128 2255 565
Size: 1563 127 2449
Fragments arranged by size:
2449 1563 127
MspA1I CmG'CkG
Cuts at: 291 596 1250 1564 1924 2937 3182 291
Size: 305 654 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 654 360 314 305 245
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 1192 1692 2599 1192
Size: 500 907 2732
Fragments arranged by size:
2732 907 500
PasI CC'CwG_GG
Cuts at: 882 882
Size: 4139
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PfoI T'CCnGG_A
Cuts at: 571 571
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 194 194
Size: 4139
PsiI TTA'TAA
Cuts at: 839 1010 839
Size: 171 3968
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 291 1564 1924 291
Size: 1273 360 2506
Fragments arranged by size:
2506 1273 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SanDI GG'GwC_CC
Cuts at: 194 194
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 1182 1867 2860 3051 33
Size: 1149 685 993 191 1121
Fragments arranged by size:
1149 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 199 1161 199
Size: 962 3177
SmlI C'TyrA_G
Cuts at: 1294 2209 2701 2963 1294
Size: 915 492 262 2470
Fragments arranged by size:
2470 915 492 262
SpeI A'CTAG_T
Cuts at: 67 805 67
Size: 738 3401
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StyI C'CwwG_G
Cuts at: 1051 1273 1627 1051
Size: 222 354 3563
Fragments arranged by size:
3563 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 687 1722 1855 2314 2523 687
Size: 1035 133 459 209 2303
Fragments arranged by size:
2303 1035 459 209 133
TatI w'GTAC_w
Cuts at: 209 418 3654 209
Size: 209 3236 694
Fragments arranged by size:
3236 694 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XmaI C'CCGG_G
Cuts at: 197 1159 197
Size: 962 3177
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BssHII BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI