Restriction map for
pF1KE0845
(Circular) (Six-base) MAPSORT of: pF1KE0845.seq Check: 4925 from: 1 to: 4055
pF1KE0845 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 799 1880 2312 3059 3327 310
Size: 489 1081 432 747 268 1038
Fragments arranged by size:
1081 1038 747 489 432 268
AflII C'TTAA_G
Cuts at: 880 880
Size: 4055
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 124 124
Size: 4055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 651 683 651
Size: 32 4023
AlwNI CAG_nnn'CTG
Cuts at: 168 1360 2927 168
Size: 1192 1567 1296
Fragments arranged by size:
1567 1296 1192
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 340 894 1059 3408 3664 340
Size: 554 165 2349 256 731
Fragments arranged by size:
2349 731 554 256 165
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 563 1069 2099 563
Size: 506 1030 2519
Fragments arranged by size:
2519 1030 506
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbsI GAAGACnn'nnnn_
Cuts at: 306 306
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 597 932 536
Size: 61 335 3659
Fragments arranged by size:
3659 335 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 439 1569 3606 6
Size: 433 1130 2037 455
Fragments arranged by size:
2037 1130 455 433
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 509
Size: 30 4025
BseYI C'CCAG_C
Cuts at: 1480 2238 2815 3311 1480
Size: 758 577 496 2224
Fragments arranged by size:
2224 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 1002 1069 1847 2037 2829 191
Size: 811 67 778 190 792 1417
Fragments arranged by size:
1417 811 792 778 190 67
Bsp1286I G_dGCh'C
Cuts at: 191 563 1002 1069 1680 1773 1847 2037 2099
Size: 372 439 67 611 93 74 190 62
Cuts at: 2099 2829 191
Size: 730 1417
Fragments arranged by size:
1417 730 611 439 372 190 93 74
67 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 506 3231 506
Size: 2725 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 685 1105 1319 1621 2002 685
Size: 420 214 302 381 2738
Fragments arranged by size:
2738 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 782 1080 1547 1570 1905 2151 3163 3249
Size: 781 298 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 781 467 449 346 335 298 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 446 1298 446
Size: 852 3203
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 110 1640 1814 2205 2232 3577 110
Size: 1530 174 391 27 1345 588
Fragments arranged by size:
1530 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 196 2078 2288 196
Size: 1882 210 1963
Fragments arranged by size:
1963 1882 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 536 597 799 932 1880 2312 3059 3327
Size: 226 61 202 133 948 432 747 268
Cuts at: 3327 310
Size: 1038
Fragments arranged by size:
1038 948 747 432 268 226 202 133
61
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 743 2074 743
Size: 1331 2724
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 351 1094 351
Size: 743 3312
HindIII A'AGCT_T
Cuts at: 809 809
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 112 1816 112
Size: 1704 2351
MslI CAynn'nnrTG
Cuts at: 124 451 940 2044 2171 124
Size: 327 489 1104 127 2008
Fragments arranged by size:
2008 1104 489 327 127
MspA1I CmG'CkG
Cuts at: 583 1166 1480 1840 2853 3098 583
Size: 583 314 360 1013 245 1540
Fragments arranged by size:
1540 1013 583 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NdeI CA'TA_TG
Cuts at: 1026 1026
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 716 824 1108 1608 2515 716
Size: 108 284 500 907 2256
Fragments arranged by size:
2256 907 500 284 108
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 1019 1102 1787 1019
Size: 83 685 3287
Fragments arranged by size:
3287 685 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 583 1480 1840 583
Size: 897 360 2798
Fragments arranged by size:
2798 897 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 196 2078 2288 196
Size: 1882 210 1963
Fragments arranged by size:
1963 1882 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 969 1015 1098 1783 2776 2967 33
Size: 936 46 83 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 191 83 46
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 880 1210 2125 2617 2879 880
Size: 330 915 492 262 2056
Fragments arranged by size:
2056 915 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 107 122 1189 1543 107
Size: 15 1067 354 2619
Fragments arranged by size:
2619 1067 354 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 1638 1771 2230 2439 288
Size: 1350 133 459 209 1904
Fragments arranged by size:
1904 1350 459 209 133
TatI w'GTAC_w
Cuts at: 278 774 3570 278
Size: 496 2796 763
Fragments arranged by size:
2796 763 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 509 541 3572 3604 355
Size: 32 122 32 3031 32 806
Fragments arranged by size:
3031 806 122 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1550 120
Size: 1430 2625
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI