(Circular) (Six-base) MAPSORT of: pF1KE0854.seq Check: 749 from: 1 to: 4109 pF1KE0854 4109 bp With 182 enzymes: SgfI * April 24, 2009 13:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 805 805 Size: 4109 AccI GT'mk_AC Cuts at: 692 1147 692 Size: 455 3654 Acc65I G'GTAC_C Cuts at: 1125 1125 Size: 4109 AclI AA'CG_TT Cuts at: 3949 3949 Size: 4109 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1934 2366 3113 3381 1934 Size: 432 747 268 2662 Fragments arranged by size: 2662 747 432 268 AflIII A'CryG_T Cuts at: 889 1461 2565 3542 889 Size: 572 1104 977 1456 Fragments arranged by size: 1456 1104 977 572 AgeI A'CCGG_T Cuts at: 2497 2497 Size: 4109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 187 187 Size: 4109 AlwNI CAG_nnn'CTG Cuts at: 590 1414 2981 590 Size: 824 1567 1718 Fragments arranged by size: 1718 1567 824 ApaLI G'TGCA_C Cuts at: 2879 2879 Size: 4109 ApoI r'AATT_y Cuts at: 851 1113 3462 3718 851 Size: 262 2349 256 1242 Fragments arranged by size: 2349 1242 262 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4109 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4109 AvaI C'yCGr_G Cuts at: 1129 1129 Size: 4109 BamHI G'GATC_C Cuts at: 1134 1134 Size: 4109 BanI G'GyrC_C Cuts at: 610 869 1125 1539 1787 1822 610 Size: 259 256 414 248 35 2897 Fragments arranged by size: 2897 414 259 256 248 35 BanII G_rGCy'C Cuts at: 302 971 1123 2153 302 Size: 669 152 1030 2258 Fragments arranged by size: 2258 1030 669 152 BbeI G_GCGC'C Cuts at: 873 1543 1791 873 Size: 670 248 3191 Fragments arranged by size: 3191 670 248 BciVI GTATCCnnnnn_n' Cuts at: 2007 2768 4109 2007 Size: 761 1341 2007 Fragments arranged by size: 2007 1341 761 BclI T'GATC_A Cuts at: 1629 1629 Size: 4109 BglI GCCn_nnn'nGGC Cuts at: 745 745 Size: 4109 BglII A'GATC_T Cuts at: 1624 3661 1624 Size: 2037 2072 BlpI GC'TnA_GC Cuts at: 1221 1221 Size: 4109 Bme1580I G_kGCm'C Cuts at: 932 1734 1827 2883 932 Size: 802 93 1056 2158 Fragments arranged by size: 2158 1056 802 93 BmrI ACTGGGnnnn_n' Cuts at: 1321 1402 1737 3881 1321 Size: 81 335 2144 1549 Fragments arranged by size: 2144 1549 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 265 2476 265 Size: 2211 1898 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1285 2164 2656 2954 1285 Size: 879 492 298 2440 Fragments arranged by size: 2440 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3813 3813 Size: 4109 BsaAI yAC'GTr Cuts at: 890 1462 890 Size: 572 3537 BsaBI GATnn'nnATC Cuts at: 6 1623 3660 6 Size: 1617 2037 455 Fragments arranged by size: 2037 1617 455 BsaHI Gr'CG_yC Cuts at: 870 1540 1788 2490 3986 870 Size: 670 248 702 1496 993 Fragments arranged by size: 1496 993 702 670 248 BsaWI w'CCGG_w Cuts at: 1298 1819 2497 2771 2918 3329 1298 Size: 521 678 274 147 411 2078 Fragments arranged by size: 2078 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 268 268 Size: 4109 BseYI C'CCAG_C Cuts at: 237 618 1534 2292 2869 3365 237 Size: 381 916 758 577 496 981 Fragments arranged by size: 981 916 758 577 496 381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 205 918 1114 205 Size: 713 196 3200 Fragments arranged by size: 3200 713 196 BsiEI CG_ry'CG Cuts at: 104 1651 1697 2905 104 Size: 1547 46 1208 1308 Fragments arranged by size: 1547 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1123 1901 2091 2883 1123 Size: 778 190 792 2349 Fragments arranged by size: 2349 792 778 190 Bsp1286I G_dGCh'C Cuts at: 302 932 971 1123 1734 1827 1901 2091 2153 Size: 630 39 152 611 93 74 190 62 Cuts at: 2153 2883 302 Size: 730 1528 Fragments arranged by size: 1528 730 630 611 190 152 93 74 62 39 BspEI T'CCGG_A Cuts at: 3329 3329 Size: 4109 BspHI T'CATG_A Cuts at: 575 3285 575 Size: 2710 1399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 805 956 1159 1373 1675 2056 805 Size: 151 203 214 302 381 2858 Fragments arranged by size: 2858 381 302 214 203 151 BsrBI CCG'CTC Cuts at: 50 2401 2455 3939 4104 50 Size: 2351 54 1484 165 55 Fragments arranged by size: 2351 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 882 2021 882 Size: 1139 2970 BsrFI r'CCGG_y Cuts at: 2107 2288 2497 2107 Size: 181 209 3719 Fragments arranged by size: 3719 209 181 BssSI C'ACGA_G Cuts at: 2380 2738 2380 Size: 358 3751 BstAPI GCAn_nnn'nTGC Cuts at: 1486 1486 Size: 4109 BstBI TT'CG_AA Cuts at: 2470 2470 Size: 4109 BstEII G'GTnAC_C Cuts at: 762 762 Size: 4109 BstXI CCAn_nnnn'nTGG Cuts at: 414 414 Size: 4109 BstYI r'GATC_y Cuts at: 1 1134 1601 1624 1959 2205 3217 3303 3315 Size: 1133 467 23 335 246 1012 86 12 Cuts at: 3315 3661 1 Size: 346 449 Fragments arranged by size: 1133 1012 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2059 2240 2403 2059 Size: 181 163 3765 Fragments arranged by size: 3765 181 163 BtsI GCAGTG_nn' Cuts at: 1352 1352 Size: 4109 DraI TTT'AAA Cuts at: 1108 1108 Size: 4109 DraIII CAC_nnn'GTG Cuts at: 1040 1040 Size: 4109 DrdI GACnn_nn'nnGTC Cuts at: 195 1815 2673 195 Size: 1620 858 1631 Fragments arranged by size: 1631 1620 858 EaeI y'GGCC_r Cuts at: 746 1694 1868 2259 2286 3631 746 Size: 948 174 391 27 1345 1224 Fragments arranged by size: 1345 1224 948 391 174 27 EagI C'GGCC_G Cuts at: 1694 1694 Size: 4109 EarI CTCTTCn'nnn_ Cuts at: 256 2132 2342 256 Size: 1876 210 2023 Fragments arranged by size: 2023 1876 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2317 2627 2773 3919 2317 Size: 310 146 1146 2507 Fragments arranged by size: 2507 1146 310 146 EcoICRI GAG'CTC Cuts at: 1121 1121 Size: 4109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1934 2366 3113 3381 1934 Size: 432 747 268 2662 Fragments arranged by size: 2662 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1248 1248 Size: 4109 EcoRI G'AATT_C Cuts at: 851 1113 851 Size: 262 3847 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 4109 FspI TGC'GCA Cuts at: 1890 1890 Size: 4109 HaeII r_GCGC'y Cuts at: 247 873 1543 1791 2813 3792 247 Size: 626 670 248 1022 979 564 Fragments arranged by size: 1022 979 670 626 564 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 614 646 1951 1981 1983 2013 614 Size: 32 1305 30 2 30 2710 Fragments arranged by size: 2710 1305 32 30 30 2 HincII GTy'rAC Cuts at: 693 1148 693 Size: 455 3654 KasI G'GCGC_C Cuts at: 869 1539 1787 869 Size: 670 248 3191 Fragments arranged by size: 3191 670 248 KpnI G_GTAC'C Cuts at: 1129 1129 Size: 4109 MluI A'CGCG_T Cuts at: 3542 3542 Size: 4109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 492 2780 2964 492 Size: 2288 184 1637 Fragments arranged by size: 2288 1637 184 MscI TGG'CCA Cuts at: 1870 1870 Size: 4109 MslI CAynn'nnrTG Cuts at: 211 520 2098 2225 211 Size: 309 1578 127 2095 Fragments arranged by size: 2095 1578 309 127 MspA1I CmG'CkG Cuts at: 237 1220 1534 1894 2907 3152 237 Size: 983 314 360 1013 245 1194 Fragments arranged by size: 1194 1013 983 360 314 245 NaeI GCC'GGC Cuts at: 2290 2290 Size: 4109 NarI GG'CG_CC Cuts at: 870 1540 1788 870 Size: 670 248 3191 Fragments arranged by size: 3191 670 248 NgoMIV G'CCGG_C Cuts at: 2288 2288 Size: 4109 NspI r_CATG'y Cuts at: 1162 1662 2569 1162 Size: 500 907 2702 Fragments arranged by size: 2702 907 500 PciI A'CATG_T Cuts at: 2565 2565 Size: 4109 PflMI CCAn_nnn'nTGG Cuts at: 489 489 Size: 4109 PmeI GTTT'AAAC Cuts at: 1108 1108 Size: 4109 PmlI CAC'GTG Cuts at: 890 890 Size: 4109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3248 3280 3248 Size: 32 4077 PstI C_TGCA'G Cuts at: 1156 1841 1156 Size: 685 3424 PvuI CG_AT'CG Cuts at: 104 104 Size: 4109 PvuII CAG'CTG Cuts at: 237 1534 1894 237 Size: 1297 360 2452 Fragments arranged by size: 2452 1297 360 RsrII CG'GwC_CG Cuts at: 2304 2304 Size: 4109 SacI G_AGCT'C Cuts at: 1123 1123 Size: 4109 SalI G'TCGA_C Cuts at: 691 1146 691 Size: 455 3654 SapI GCTCTTCn'nnn_ Cuts at: 2132 2342 2132 Size: 210 3899 SbfI CC_TGCA'GG Cuts at: 1156 1156 Size: 4109 ScaI AGT'ACT Cuts at: 3626 3626 Size: 4109 SfcI C'TryA_G Cuts at: 33 1152 1837 2830 3021 33 Size: 1119 685 993 191 1121 Fragments arranged by size: 1121 1119 993 685 191 SfoI GGC'GCC Cuts at: 871 1541 1789 871 Size: 670 248 3191 Fragments arranged by size: 3191 670 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4109 SmaI CCC'GGG Cuts at: 1131 1131 Size: 4109 SmlI C'TyrA_G Cuts at: 1264 2179 2671 2933 1264 Size: 915 492 262 2440 Fragments arranged by size: 2440 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4109 SphI G_CATG'C Cuts at: 1162 1662 1162 Size: 500 3609 StyI C'CwwG_G Cuts at: 1243 1597 1243 Size: 354 3755 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 242 1692 1825 2284 2493 242 Size: 1450 133 459 209 1858 Fragments arranged by size: 1858 1450 459 209 133 TatI w'GTAC_w Cuts at: 496 3624 496 Size: 3128 981 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3626 3658 3626 Size: 32 4077 Tth111I GACn'n_nGTC Cuts at: 1906 1906 Size: 4109 XbaI T'CTAG_A Cuts at: 1140 1140 Size: 4109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 756 1604 756 Size: 848 3261 XmaI C'CCGG_G Cuts at: 1129 1129 Size: 4109 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI