Restriction map for pF1KE0854
 (Circular) (Six-base) MAPSORT of: pF1KE0854.seq  Check: 749  from: 1  to: 4109



pF1KE0854  4109 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    805     805 
   Size:       4109
 
AccI GT'mk_AC

Cuts at:    692    1147     692 
   Size:        455    3654
 
Acc65I G'GTAC_C

Cuts at:   1125    1125 
   Size:       4109
 
AclI AA'CG_TT

Cuts at:   3949    3949 
   Size:       4109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1934    2366    3113    3381    1934 
   Size:        432     747     268    2662

  Fragments arranged by size: 

                2662     747     432     268
 
AflIII A'CryG_T

Cuts at:    889    1461    2565    3542     889 
   Size:        572    1104     977    1456

  Fragments arranged by size: 

                1456    1104     977     572
 
AgeI A'CCGG_T

Cuts at:   2497    2497 
   Size:       4109
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    187     187 
   Size:       4109
 
AlwNI CAG_nnn'CTG

Cuts at:    590    1414    2981     590 
   Size:        824    1567    1718

  Fragments arranged by size: 

                1718    1567     824
 
ApaLI G'TGCA_C

Cuts at:   2879    2879 
   Size:       4109
 
ApoI r'AATT_y

Cuts at:    851    1113    3462    3718     851 
   Size:        262    2349     256    1242

  Fragments arranged by size: 

                2349    1242     262     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4109
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4109
 
AvaI C'yCGr_G

Cuts at:   1129    1129 
   Size:       4109
 
BamHI G'GATC_C

Cuts at:   1134    1134 
   Size:       4109
 
BanI G'GyrC_C

Cuts at:    610     869    1125    1539    1787    1822     610 
   Size:        259     256     414     248      35    2897

  Fragments arranged by size: 

                2897     414     259     256     248      35
 
BanII G_rGCy'C

Cuts at:    302     971    1123    2153     302 
   Size:        669     152    1030    2258

  Fragments arranged by size: 

                2258    1030     669     152
 
BbeI G_GCGC'C

Cuts at:    873    1543    1791     873 
   Size:        670     248    3191

  Fragments arranged by size: 

                3191     670     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2007    2768    4109    2007 
   Size:        761    1341    2007

  Fragments arranged by size: 

                2007    1341     761
 
BclI T'GATC_A

Cuts at:   1629    1629 
   Size:       4109
 
BglI GCCn_nnn'nGGC

Cuts at:    745     745 
   Size:       4109
 
BglII A'GATC_T

Cuts at:   1624    3661    1624 
   Size:       2037    2072
 
BlpI GC'TnA_GC

Cuts at:   1221    1221 
   Size:       4109
 
Bme1580I G_kGCm'C

Cuts at:    932    1734    1827    2883     932 
   Size:        802      93    1056    2158

  Fragments arranged by size: 

                2158    1056     802      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1321    1402    1737    3881    1321 
   Size:         81     335    2144    1549

  Fragments arranged by size: 

                2144    1549     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    265    2476     265 
   Size:       2211    1898
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1285    2164    2656    2954    1285 
   Size:        879     492     298    2440

  Fragments arranged by size: 

                2440     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3813    3813 
   Size:       4109
 
BsaAI yAC'GTr

Cuts at:    890    1462     890 
   Size:        572    3537
 
BsaBI GATnn'nnATC

Cuts at:      6    1623    3660       6 
   Size:       1617    2037     455

  Fragments arranged by size: 

                2037    1617     455
 
BsaHI Gr'CG_yC

Cuts at:    870    1540    1788    2490    3986     870 
   Size:        670     248     702    1496     993

  Fragments arranged by size: 

                1496     993     702     670     248
 
BsaWI w'CCGG_w

Cuts at:   1298    1819    2497    2771    2918    3329    1298 
   Size:        521     678     274     147     411    2078

  Fragments arranged by size: 

                2078     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    268     268 
   Size:       4109
 
BseYI C'CCAG_C

Cuts at:    237     618    1534    2292    2869    3365     237 
   Size:        381     916     758     577     496     981

  Fragments arranged by size: 

                 981     916     758     577     496     381
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    205     918    1114     205 
   Size:        713     196    3200

  Fragments arranged by size: 

                3200     713     196
 
BsiEI CG_ry'CG

Cuts at:    104    1651    1697    2905     104 
   Size:       1547      46    1208    1308

  Fragments arranged by size: 

                1547    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1123    1901    2091    2883    1123 
   Size:        778     190     792    2349

  Fragments arranged by size: 

                2349     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    302     932     971    1123    1734    1827    1901    2091    2153 
   Size:        630      39     152     611      93      74     190      62

Cuts at:   2153    2883     302 
   Size:        730    1528

  Fragments arranged by size: 

                1528     730     630     611     190     152      93      74
                  62      39
 
BspEI T'CCGG_A

Cuts at:   3329    3329 
   Size:       4109
 
BspHI T'CATG_A

Cuts at:    575    3285     575 
   Size:       2710    1399
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    805     956    1159    1373    1675    2056     805 
   Size:        151     203     214     302     381    2858

  Fragments arranged by size: 

                2858     381     302     214     203     151
 
BsrBI CCG'CTC

Cuts at:     50    2401    2455    3939    4104      50 
   Size:       2351      54    1484     165      55

  Fragments arranged by size: 

                2351    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    882    2021     882 
   Size:       1139    2970
 
BsrFI r'CCGG_y

Cuts at:   2107    2288    2497    2107 
   Size:        181     209    3719

  Fragments arranged by size: 

                3719     209     181
 
BssSI C'ACGA_G

Cuts at:   2380    2738    2380 
   Size:        358    3751
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1486    1486 
   Size:       4109
 
BstBI TT'CG_AA

Cuts at:   2470    2470 
   Size:       4109
 
BstEII G'GTnAC_C

Cuts at:    762     762 
   Size:       4109
 
BstXI CCAn_nnnn'nTGG

Cuts at:    414     414 
   Size:       4109
 
BstYI r'GATC_y

Cuts at:      1    1134    1601    1624    1959    2205    3217    3303    3315 
   Size:       1133     467      23     335     246    1012      86      12

Cuts at:   3315    3661       1 
   Size:        346     449

  Fragments arranged by size: 

                1133    1012     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2059    2240    2403    2059 
   Size:        181     163    3765

  Fragments arranged by size: 

                3765     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1352    1352 
   Size:       4109
 
DraI TTT'AAA

Cuts at:   1108    1108 
   Size:       4109
 
DraIII CAC_nnn'GTG

Cuts at:   1040    1040 
   Size:       4109
 
DrdI GACnn_nn'nnGTC

Cuts at:    195    1815    2673     195 
   Size:       1620     858    1631

  Fragments arranged by size: 

                1631    1620     858
 
EaeI y'GGCC_r

Cuts at:    746    1694    1868    2259    2286    3631     746 
   Size:        948     174     391      27    1345    1224

  Fragments arranged by size: 

                1345    1224     948     391     174      27
 
EagI C'GGCC_G

Cuts at:   1694    1694 
   Size:       4109
 
EarI CTCTTCn'nnn_

Cuts at:    256    2132    2342     256 
   Size:       1876     210    2023

  Fragments arranged by size: 

                2023    1876     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2317    2627    2773    3919    2317 
   Size:        310     146    1146    2507

  Fragments arranged by size: 

                2507    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1121    1121 
   Size:       4109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1934    2366    3113    3381    1934 
   Size:        432     747     268    2662

  Fragments arranged by size: 

                2662     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1248    1248 
   Size:       4109
 
EcoRI G'AATT_C

Cuts at:    851    1113     851 
   Size:        262    3847
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2128    2128 
   Size:       4109
 
FspI TGC'GCA

Cuts at:   1890    1890 
   Size:       4109
 
HaeII r_GCGC'y

Cuts at:    247     873    1543    1791    2813    3792     247 
   Size:        626     670     248    1022     979     564

  Fragments arranged by size: 

                1022     979     670     626     564     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    614     646    1951    1981    1983    2013     614 
   Size:         32    1305      30       2      30    2710

  Fragments arranged by size: 

                2710    1305      32      30      30       2
 
HincII GTy'rAC

Cuts at:    693    1148     693 
   Size:        455    3654
 
KasI G'GCGC_C

Cuts at:    869    1539    1787     869 
   Size:        670     248    3191

  Fragments arranged by size: 

                3191     670     248
 
KpnI G_GTAC'C

Cuts at:   1129    1129 
   Size:       4109
 
MluI A'CGCG_T

Cuts at:   3542    3542 
   Size:       4109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    492    2780    2964     492 
   Size:       2288     184    1637

  Fragments arranged by size: 

                2288    1637     184
 
MscI TGG'CCA

Cuts at:   1870    1870 
   Size:       4109
 
MslI CAynn'nnrTG

Cuts at:    211     520    2098    2225     211 
   Size:        309    1578     127    2095

  Fragments arranged by size: 

                2095    1578     309     127
 
MspA1I CmG'CkG

Cuts at:    237    1220    1534    1894    2907    3152     237 
   Size:        983     314     360    1013     245    1194

  Fragments arranged by size: 

                1194    1013     983     360     314     245
 
NaeI GCC'GGC

Cuts at:   2290    2290 
   Size:       4109
 
NarI GG'CG_CC

Cuts at:    870    1540    1788     870 
   Size:        670     248    3191

  Fragments arranged by size: 

                3191     670     248
 
NgoMIV G'CCGG_C

Cuts at:   2288    2288 
   Size:       4109
 
NspI r_CATG'y

Cuts at:   1162    1662    2569    1162 
   Size:        500     907    2702

  Fragments arranged by size: 

                2702     907     500
 
PciI A'CATG_T

Cuts at:   2565    2565 
   Size:       4109
 
PflMI CCAn_nnn'nTGG

Cuts at:    489     489 
   Size:       4109
 
PmeI GTTT'AAAC

Cuts at:   1108    1108 
   Size:       4109
 
PmlI CAC'GTG

Cuts at:    890     890 
   Size:       4109
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3248    3280    3248 
   Size:         32    4077
 
PstI C_TGCA'G

Cuts at:   1156    1841    1156 
   Size:        685    3424
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4109
 
PvuII CAG'CTG

Cuts at:    237    1534    1894     237 
   Size:       1297     360    2452

  Fragments arranged by size: 

                2452    1297     360
 
RsrII CG'GwC_CG

Cuts at:   2304    2304 
   Size:       4109
 
SacI G_AGCT'C

Cuts at:   1123    1123 
   Size:       4109
 
SalI G'TCGA_C

Cuts at:    691    1146     691 
   Size:        455    3654
 
SapI GCTCTTCn'nnn_

Cuts at:   2132    2342    2132 
   Size:        210    3899
 
SbfI CC_TGCA'GG

Cuts at:   1156    1156 
   Size:       4109
 
ScaI AGT'ACT

Cuts at:   3626    3626 
   Size:       4109
 
SfcI C'TryA_G

Cuts at:     33    1152    1837    2830    3021      33 
   Size:       1119     685     993     191    1121

  Fragments arranged by size: 

                1121    1119     993     685     191
 
SfoI GGC'GCC

Cuts at:    871    1541    1789     871 
   Size:        670     248    3191

  Fragments arranged by size: 

                3191     670     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4109
 
SmaI CCC'GGG

Cuts at:   1131    1131 
   Size:       4109
 
SmlI C'TyrA_G

Cuts at:   1264    2179    2671    2933    1264 
   Size:        915     492     262    2440

  Fragments arranged by size: 

                2440     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4109
 
SphI G_CATG'C

Cuts at:   1162    1662    1162 
   Size:        500    3609
 
StyI C'CwwG_G

Cuts at:   1243    1597    1243 
   Size:        354    3755
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    242    1692    1825    2284    2493     242 
   Size:       1450     133     459     209    1858

  Fragments arranged by size: 

                1858    1450     459     209     133
 
TatI w'GTAC_w

Cuts at:    496    3624     496 
   Size:       3128     981
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3626    3658    3626 
   Size:         32    4077
 
Tth111I GACn'n_nGTC

Cuts at:   1906    1906 
   Size:       4109
 
XbaI T'CTAG_A

Cuts at:   1140    1140 
   Size:       4109
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    756    1604     756 
   Size:        848    3261
 
XmaI C'CCGG_G

Cuts at:   1129    1129 
   Size:       4109
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
    PflMI     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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