Restriction map for
pF1KE0865
(Circular) (Six-base) MAPSORT of: pF1KE0865.seq Check: 5162 from: 1 to: 4118
pF1KE0865 4118 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 573 895 573
Size: 322 3796
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1943 2375 3122 3390 865
Size: 1078 432 747 268 1593
Fragments arranged by size:
1593 1078 747 432 268
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AleI CACnn'nnGTG
Cuts at: 902 902
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1423 2990 1423
Size: 1567 2551
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 116 199 233 648 1122 3471 3727 116
Size: 83 34 415 474 2349 256 507
Fragments arranged by size:
2349 507 474 415 256 83 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 1134 1548 1796 1831 1134
Size: 414 248 35 3421
Fragments arranged by size:
3421 414 248 35
BanII G_rGCy'C
Cuts at: 1132 2162 1132
Size: 1030 3088
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbvCI CC'TCA_GC
Cuts at: 855 860 855
Size: 5 4113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4084
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglII A'GATC_T
Cuts at: 167 1633 3670 167
Size: 1466 2037 615
Fragments arranged by size:
2037 1466 615
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 1743 1836 2892 1743
Size: 93 1056 2969
Fragments arranged by size:
2969 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
Bpu10I CC'TnA_GC
Cuts at: 855 860 855
Size: 5 4113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2173 2665 2963 1294
Size: 879 492 298 2449
Fragments arranged by size:
2449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 3822 370
Size: 3452 666
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 412 1632 3669 6
Size: 406 1220 2037 455
Fragments arranged by size:
2037 1220 455 406
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 767 797 767
Size: 30 4088
BseYI C'CCAG_C
Cuts at: 210 1543 2301 2878 3374 210
Size: 1333 758 577 496 954
Fragments arranged by size:
1333 954 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 462 1132 1910 2100 2892 462
Size: 670 778 190 792 1688
Fragments arranged by size:
1688 792 778 670 190
BsmI GAATG_Cn'
Cuts at: 359 827 359
Size: 468 3650
Bsp1286I G_dGCh'C
Cuts at: 462 1132 1743 1836 1910 2100 2162 2892 462
Size: 670 611 93 74 190 62 730 1688
Fragments arranged by size:
1688 730 670 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 573 895 1168 1382 1684 2065 573
Size: 322 273 214 302 381 2626
Fragments arranged by size:
2626 381 322 302 273 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 2030 297
Size: 1733 2385
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstEII G'GTnAC_C
Cuts at: 192 192
Size: 4118
BstYI r'GATC_y
Cuts at: 1 167 1143 1610 1633 1968 2214 3226 3312
Size: 166 976 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 976 467 449 346 335 246 166
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 946 946
Size: 4118
BtgI C'CryG_G
Cuts at: 188 1028 188
Size: 840 3278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 431 1703 1877 2268 2295 3640 431
Size: 1272 174 391 27 1345 909
Fragments arranged by size:
1345 1272 909 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 467 2141 2351 467
Size: 1674 210 2234
Fragments arranged by size:
2234 1674 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1943 2375 3122 3390 865
Size: 1078 432 747 268 1593
Fragments arranged by size:
1593 1078 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1257 1257
Size: 4118
EcoRI G'AATT_C
Cuts at: 199 1122 199
Size: 923 3195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 680 1552 1800 2822 3801 680
Size: 872 248 1022 979 997
Fragments arranged by size:
1022 997 979 872 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 996 1028 1960 1990 1992 2022 996
Size: 32 932 30 2 30 3092
Fragments arranged by size:
3092 932 32 30 30 2
HincII GTy'rAC
Cuts at: 750 1157 750
Size: 407 3711
HpaI GTT'AAC
Cuts at: 750 750
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MfeI C'AATT_G
Cuts at: 614 614
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 1083 2789 2973 995
Size: 88 1706 184 2140
Fragments arranged by size:
2140 1706 184 88
MscI TGG'CCA
Cuts at: 433 1879 433
Size: 1446 2672
MslI CAynn'nnrTG
Cuts at: 340 571 902 2107 2234 340
Size: 231 331 1205 127 2224
Fragments arranged by size:
2224 1205 331 231 127
MspA1I CmG'CkG
Cuts at: 240 604 859 1229 1543 1903 2916 3161 240
Size: 364 255 370 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 370 364 360 314 255 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 188 1028 188
Size: 840 3278
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 1171 1671 2578 1171
Size: 500 907 2711
Fragments arranged by size:
2711 907 500
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 776 808 776
Size: 32 4086
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 240 604 859 1543 1903 240
Size: 364 255 684 360 2455
Fragments arranged by size:
2455 684 364 360 255
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 467 2141 2351 467
Size: 1674 210 2234
Fragments arranged by size:
2234 1674 210
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 953 1161 1846 2839 3030 33
Size: 920 208 685 993 191 1121
Fragments arranged by size:
1121 993 920 685 208 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 1273 2188 2680 2942 1273
Size: 915 492 262 2449
Fragments arranged by size:
2449 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StuI AGG'CCT
Cuts at: 951 951
Size: 4118
StyI C'CwwG_G
Cuts at: 188 225 354 480 1028 1252 1606 188
Size: 37 129 126 548 224 354 2700
Fragments arranged by size:
2700 548 354 224 129 126 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 502 1701 1834 2293 2502 502
Size: 1199 133 459 209 2118
Fragments arranged by size:
2118 1199 459 209 133
TatI w'GTAC_w
Cuts at: 344 783 3633 344
Size: 439 2850 829
Fragments arranged by size:
2850 829 439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BpmI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI