Restriction map for
pF1KE0872
(Circular) (Six-base) MAPSORT of: pF1KE0872.seq Check: 3904 from: 1 to: 4103
pF1KE0872 4103 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 992 1141 992
Size: 149 3954
Acc65I G'GTAC_C
Cuts at: 527 1119 527
Size: 592 3511
AclI AA'CG_TT
Cuts at: 1097 3943 1097
Size: 2846 1257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
AflIII A'CryG_T
Cuts at: 880 1455 2559 3536 880
Size: 575 1104 977 1447
Fragments arranged by size:
1447 1104 977 575
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 741 741
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 1408 2975 1408
Size: 1567 2536
ApaI G_GGCC'C
Cuts at: 674 791 674
Size: 117 3986
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 527 1119 1533 1781 1816 527
Size: 592 414 248 35 2814
Fragments arranged by size:
2814 592 414 248 35
BanII G_rGCy'C
Cuts at: 674 791 946 1117 2147 674
Size: 117 155 171 1030 2630
Fragments arranged by size:
2630 1030 171 155 117
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 1027 1027
Size: 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BfrBI ATG'CAT
Cuts at: 180 180
Size: 4103
BglII A'GATC_T
Cuts at: 371 1618 3655 371
Size: 1247 2037 819
Fragments arranged by size:
2037 1247 819
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 674 791 1728 1821 2877 674
Size: 117 937 93 1056 1900
Fragments arranged by size:
1900 1056 937 117 93
BmrI ACTGGGnnnn_n'
Cuts at: 490 581 1315 1396 1731 3875 490
Size: 91 734 81 335 2144 718
Fragments arranged by size:
2144 734 718 335 91 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2650 2948 1279
Size: 879 492 298 2434
Fragments arranged by size:
2434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 778 1617 3654 6
Size: 772 839 2037 455
Fragments arranged by size:
2037 839 772 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 281 311 370 400 544 574 281
Size: 30 59 30 144 30 3810
Fragments arranged by size:
3810 144 59 30 30 30
BseYI C'CCAG_C
Cuts at: 605 960 1528 2286 2863 3359 605
Size: 355 568 758 577 496 1349
Fragments arranged by size:
1349 758 577 568 496 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1117 1895 2085 2877 1117
Size: 778 190 792 2343
Fragments arranged by size:
2343 792 778 190
BsmI GAATG_Cn'
Cuts at: 974 974
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 674 791 946 1117 1728 1821 1895 2085 2147
Size: 117 155 171 611 93 74 190 62
Cuts at: 2147 2877 674
Size: 730 1900
Fragments arranged by size:
1900 730 611 190 171 155 117 93
74 62
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 566 3279 566
Size: 2713 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1153 1367 1669 2050 1153
Size: 214 302 381 3206
Fragments arranged by size:
3206 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 2015 1007
Size: 1008 3095
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BsrGI T'GTAC_A
Cuts at: 338 338
Size: 4103
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 1038 2464 1038
Size: 1426 2677
BstYI r'GATC_y
Cuts at: 1 371 808 1128 1595 1618 1953 2199 3211
Size: 370 437 320 467 23 335 246 1012
Cuts at: 3211 3297 3309 3655 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 437 370 346 335 320
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 993 993
Size: 4103
BtgI C'CryG_G
Cuts at: 213 213
Size: 4103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 274 311 1346 274
Size: 37 1035 3031
Fragments arranged by size:
3031 1035 37
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DraIII CAC_nnn'GTG
Cuts at: 693 693
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 533 1688 1862 2253 2280 3625 533
Size: 1155 174 391 27 1345 1011
Fragments arranged by size:
1345 1155 1011 391 174 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 629 2311 2621 2767 3913 629
Size: 1682 310 146 1146 819
Fragments arranged by size:
1682 1146 819 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 160 788 887 1242 160
Size: 628 99 355 3021
Fragments arranged by size:
3021 628 355 99
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 1537 1785 2807 3786 1537
Size: 248 1022 979 1854
Fragments arranged by size:
1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 969 1001 1945 1975 1977 2007 281
Size: 32 656 32 944 30 2 30 2377
Fragments arranged by size:
2377 944 656 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1082 1142 1082
Size: 60 4043
HindIII A'AGCT_T
Cuts at: 869 869
Size: 4103
HpaI GTT'AAC
Cuts at: 1082 1082
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 531 1123 531
Size: 592 3511
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 535 1864 535
Size: 1329 2774
MslI CAynn'nnrTG
Cuts at: 334 1000 2092 2219 334
Size: 666 1092 127 2218
Fragments arranged by size:
2218 1092 666 127
MspA1I CmG'CkG
Cuts at: 1214 1528 1888 2901 3146 1214
Size: 314 360 1013 245 2171
Fragments arranged by size:
2171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 213 213
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NsiI A_TGCA'T
Cuts at: 182 182
Size: 4103
NspI r_CATG'y
Cuts at: 405 884 1156 1656 2563 405
Size: 479 272 500 907 1945
Fragments arranged by size:
1945 907 500 479 272
PciI A'CATG_T
Cuts at: 880 2559 880
Size: 1679 2424
PflMI CCAn_nnn'nTGG
Cuts at: 897 897
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 160 887 160
Size: 727 3376
PspOMI G'GGCC_C
Cuts at: 670 787 670
Size: 117 3986
PstI C_TGCA'G
Cuts at: 1150 1835 1150
Size: 685 3418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SanDI GG'GwC_CC
Cuts at: 160 887 160
Size: 727 3376
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 1029 1146 1831 2824 3015 33
Size: 996 117 685 993 191 1121
Fragments arranged by size:
1121 996 993 685 191 117
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 1258 2173 2665 2927 1258
Size: 915 492 262 2434
Fragments arranged by size:
2434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StyI C'CwwG_G
Cuts at: 213 1237 1591 213
Size: 1024 354 2725
Fragments arranged by size:
2725 1024 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 1686 1819 2278 2487 348
Size: 1338 133 459 209 1964
Fragments arranged by size:
1964 1338 459 209 133
TatI w'GTAC_w
Cuts at: 338 3618 338
Size: 3280 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 544 576 3620 3652 544
Size: 32 3044 32 995
Fragments arranged by size:
3044 995 32 32
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 366 1134 366
Size: 768 3335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BseRI BsiWI BsmBI BssHII BstEII BstXI Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI MfeI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI