Restriction map for
pF1KE0875
(Circular) (Six-base) MAPSORT of: pF1KE0875.seq Check: 412 from: 1 to: 4154
pF1KE0875 4154 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 955 1170 955
Size: 215 3939
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2411 3158 3426 1979
Size: 432 747 268 2707
Fragments arranged by size:
2707 747 432 268
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 229 660 882 1158 3507 3763 229
Size: 431 222 276 2349 256 620
Fragments arranged by size:
2349 620 431 276 256 222
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 1174 1174
Size: 4154
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 955 1170 1584 1832 1867 955
Size: 215 414 248 35 3242
Fragments arranged by size:
3242 414 248 215 35
BanII G_rGCy'C
Cuts at: 1168 2198 1168
Size: 1030 3124
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 903 903
Size: 4154
BciVI GTATCCnnnnn_n'
Cuts at: 247 2052 2813 4154 247
Size: 1805 761 1341 247
Fragments arranged by size:
1805 1341 761 247
BclI T'GATC_A
Cuts at: 941 1674 941
Size: 733 3421
BglII A'GATC_T
Cuts at: 166 1669 3706 166
Size: 1503 2037 614
Fragments arranged by size:
2037 1503 614
BlpI GC'TnA_GC
Cuts at: 921 1266 921
Size: 345 3809
Bme1580I G_kGCm'C
Cuts at: 1779 1872 2928 1779
Size: 93 1056 3005
Fragments arranged by size:
3005 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4154
Bpu10I CC'TnA_GC
Cuts at: 604 604
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 974 1110 1330 2209 2701 2999 736
Size: 238 136 220 879 492 298 1891
Fragments arranged by size:
1891 879 492 298 238 220 136
BsaI GGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 4154
BsaAI yAC'GTr
Cuts at: 917 1507 917
Size: 590 3564
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 1585 1833 2535 4031 1585
Size: 248 702 1496 1708
Fragments arranged by size:
1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 649 1343 1864 2542 2816 2963 3374 649
Size: 694 521 678 274 147 411 1429
Fragments arranged by size:
1429 694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 414 414
Size: 4154
BseYI C'CCAG_C
Cuts at: 1579 2337 2914 3410 1579
Size: 758 577 496 2323
Fragments arranged by size:
2323 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 514 509
Size: 5 4149
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2950 104
Size: 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 922 1168 1946 2136 2928 922
Size: 246 778 190 792 2148
Fragments arranged by size:
2148 792 778 246 190
Bsp1286I G_dGCh'C
Cuts at: 922 1168 1779 1872 1946 2136 2198 2928 922
Size: 246 611 93 74 190 62 730 2148
Fragments arranged by size:
2148 730 611 246 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1204 1418 1720 2101 1204
Size: 214 302 381 3257
Fragments arranged by size:
3257 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 521 2066 327
Size: 194 1545 2415
Fragments arranged by size:
2415 1545 194
BsrFI r'CCGG_y
Cuts at: 2152 2333 2542 2152
Size: 181 209 3764
Fragments arranged by size:
3764 209 181
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstYI r'GATC_y
Cuts at: 1 166 1179 1646 1669 2004 2250 3262 3348
Size: 165 1013 467 23 335 246 1012 86
Cuts at: 3348 3360 3706 1
Size: 12 346 449
Fragments arranged by size:
1013 1012 467 449 346 335 246 165
86 23 12
BtgI C'CryG_G
Cuts at: 791 791
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 497 1397 497
Size: 900 3254
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 222 1860 2718 222
Size: 1638 858 1658
Fragments arranged by size:
1658 1638 858
EaeI y'GGCC_r
Cuts at: 569 1739 1913 2304 2331 3676 569
Size: 1170 174 391 27 1345 1047
Fragments arranged by size:
1345 1170 1047 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1166 1166
Size: 4154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2411 3158 3426 1979
Size: 432 747 268 2707
Fragments arranged by size:
2707 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 607 607
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 1293 1293
Size: 4154
EcoRI G'AATT_C
Cuts at: 882 1158 882
Size: 276 3878
EcoRV GAT'ATC
Cuts at: 408 800 408
Size: 392 3762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 581 613 1996 2026 2028 2058 581
Size: 32 1383 30 2 30 2677
Fragments arranged by size:
2677 1383 32 30 30 2
HincII GTy'rAC
Cuts at: 524 1193 524
Size: 669 3485
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 959 1174 959
Size: 215 3939
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 571 1915 571
Size: 1344 2810
MslI CAynn'nnrTG
Cuts at: 370 793 2143 2270 370
Size: 423 1350 127 2254
Fragments arranged by size:
2254 1350 423 127
MspA1I CmG'CkG
Cuts at: 667 1265 1579 1939 2952 3197 667
Size: 598 314 360 1013 245 1624
Fragments arranged by size:
1624 1013 598 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NcoI C'CATG_G
Cuts at: 791 791
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 734 1207 1707 2614 734
Size: 473 500 907 2274
Fragments arranged by size:
2274 907 500 473
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PfoI T'CCnGG_A
Cuts at: 401 401
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PmlI CAC'GTG
Cuts at: 917 917
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 667 1579 1939 667
Size: 912 360 2882
Fragments arranged by size:
2882 912 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 1168 1168
Size: 4154
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1083 1197 1882 2875 3066 33
Size: 1050 114 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191 114
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 751 989 1089 1309 2224 2716 2978 751
Size: 238 100 220 915 492 262 1927
Fragments arranged by size:
1927 915 492 262 238 220 100
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
SspI AAT'ATT
Cuts at: 714 740 783 1123 714
Size: 26 43 340 3745
Fragments arranged by size:
3745 340 43 26
StyI C'CwwG_G
Cuts at: 791 1288 1642 791
Size: 497 354 3303
Fragments arranged by size:
3303 497 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 1077 1737 1870 2329 2538 190
Size: 887 660 133 459 209 1806
Fragments arranged by size:
1806 887 660 459 209 133
TatI w'GTAC_w
Cuts at: 127 374 3669 127
Size: 247 3295 612
Fragments arranged by size:
3295 612 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 476 508 3671 3703 476
Size: 32 3163 32 927
Fragments arranged by size:
3163 927 32 32
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
XmnI GAAnn'nnTTC
Cuts at: 233 471 660 233
Size: 238 189 3727
Fragments arranged by size:
3727 238 189
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI