Restriction map for
pF1KE0876
(Circular) (Six-base) MAPSORT of: pF1KE0876.seq Check: 102 from: 1 to: 4151
pF1KE0876 4151 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 178 178
Size: 4151
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 2408 3155 3423 1976
Size: 432 747 268 2704
Fragments arranged by size:
2704 747 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 653 1144 653
Size: 491 3660
AlwNI CAG_nnn'CTG
Cuts at: 1456 3023 1456
Size: 1567 2584
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 235 743 1155 3504 3760 235
Size: 508 412 2349 256 626
Fragments arranged by size:
2349 626 508 412 256
AseI AT'TA_AT
Cuts at: 20 887 20
Size: 867 3284
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1171 1171
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 272 529 1165 2195 272
Size: 257 636 1030 2228
Fragments arranged by size:
2228 1030 636 257
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbvCI CC'TCA_GC
Cuts at: 896 896
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 726 1671 726
Size: 945 3206
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 1776 1869 2925 1776
Size: 93 1056 3002
Fragments arranged by size:
3002 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4151
Bpu10I CC'TnA_GC
Cuts at: 896 1117 896
Size: 221 3930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 4151
BseYI C'CCAG_C
Cuts at: 179 1576 2334 2911 3407 179
Size: 1397 758 577 496 923
Fragments arranged by size:
1397 923 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 272 498 1165 1943 2133 2925 272
Size: 226 667 778 190 792 1498
Fragments arranged by size:
1498 792 778 667 226 190
Bsp1286I G_dGCh'C
Cuts at: 272 498 529 1165 1776 1869 1943 2133 2195
Size: 226 31 636 611 93 74 190 62
Cuts at: 2195 2925 272
Size: 730 1498
Fragments arranged by size:
1498 730 636 611 226 190 93 74
62 31
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 608 797 3327 608
Size: 189 2530 1432
Fragments arranged by size:
2530 1432 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 1201 1415 1717 2098 178
Size: 1023 214 302 381 2231
Fragments arranged by size:
2231 1023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BsrGI T'GTAC_A
Cuts at: 110 305 977 110
Size: 195 672 3284
Fragments arranged by size:
3284 672 195
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstEII G'GTnAC_C
Cuts at: 190 190
Size: 4151
BstYI r'GATC_y
Cuts at: 1 1176 1643 1666 2001 2247 3259 3345 3357
Size: 1175 467 23 335 246 1012 86 12
Cuts at: 3357 3703 1
Size: 346 449
Fragments arranged by size:
1175 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1078 1078
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DraIII CAC_nnn'GTG
Cuts at: 536 573 536
Size: 37 4114
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 575 1736 1910 2301 2328 3673 575
Size: 1161 174 391 27 1345 1053
Fragments arranged by size:
1345 1161 1053 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 876 2174 2384 876
Size: 1298 210 2643
Fragments arranged by size:
2643 1298 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 270 1163 270
Size: 893 3258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1976 2408 3155 3423 256
Size: 1720 432 747 268 984
Fragments arranged by size:
1720 984 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1290 1290
Size: 4151
EcoRI G'AATT_C
Cuts at: 235 1155 235
Size: 920 3231
EcoRV GAT'ATC
Cuts at: 777 777
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 2170 930
Size: 1240 2911
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 1110 1142 1993 2023 2025 2055 457
Size: 32 621 32 851 30 2 30 2553
Fragments arranged by size:
2553 851 621 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 577 1912 577
Size: 1335 2816
MslI CAynn'nnrTG
Cuts at: 307 607 787 802 2140 2267 307
Size: 300 180 15 1338 127 2191
Fragments arranged by size:
2191 1338 300 180 127 15
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 573 573
Size: 4151
PfoI T'CCnGG_A
Cuts at: 646 646
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PstI C_TGCA'G
Cuts at: 503 1198 1883 503
Size: 695 685 2771
Fragments arranged by size:
2771 695 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 272 1165 272
Size: 893 3258
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 499 1071 1194 1879 2872 3063 33
Size: 466 572 123 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 466 191 123
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1306 2221 2713 2975 1306
Size: 915 492 262 2482
Fragments arranged by size:
2482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
SspI AAT'ATT
Cuts at: 813 813
Size: 4151
StyI C'CwwG_G
Cuts at: 516 1285 1639 516
Size: 769 354 3028
Fragments arranged by size:
3028 769 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 110 305 977 3666 110
Size: 195 672 2689 595
Fragments arranged by size:
2689 672 595 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
XmnI GAAnn'nnTTC
Cuts at: 239 913 239
Size: 674 3477
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
BtgI ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI