Restriction map for
pF1KE0879
(Circular) (Six-base) MAPSORT of: pF1KE0879.seq Check: 5119 from: 1 to: 4037
pF1KE0879 4037 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1862 2294 3041 3309 1862
Size: 432 747 268 2590
Fragments arranged by size:
2590 747 432 268
AflIII A'CryG_T
Cuts at: 182 823 1389 2493 3470 182
Size: 641 566 1104 977 749
Fragments arranged by size:
1104 977 749 641 566
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AleI CACnn'nnGTG
Cuts at: 130 196 608 130
Size: 66 412 3559
Fragments arranged by size:
3559 412 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 1342 2909 1342
Size: 1567 2470
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 691 1041 3390 3646 691
Size: 350 2349 256 1082
Fragments arranged by size:
2349 1082 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 1053 1467 1715 1750 1053
Size: 414 248 35 3340
Fragments arranged by size:
3340 414 248 35
BanII G_rGCy'C
Cuts at: 1051 2081 1051
Size: 1030 3007
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbvCI CC'TCA_GC
Cuts at: 214 214
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 1662 1755 2811 1662
Size: 93 1056 2888
Fragments arranged by size:
2888 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 517 1249 1330 1665 3809 517
Size: 732 81 335 2144 745
Fragments arranged by size:
2144 745 732 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 2404 666
Size: 1738 2299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 781
Size: 4037
Bpu10I CC'TnA_GC
Cuts at: 214 214
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1213 2092 2584 2882 793
Size: 420 879 492 298 1948
Fragments arranged by size:
1948 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 703 1551 3588 6
Size: 697 848 2037 455
Fragments arranged by size:
2037 848 697 455
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 739
Size: 4037
BseYI C'CCAG_C
Cuts at: 1462 2220 2797 3293 1462
Size: 758 577 496 2206
Fragments arranged by size:
2206 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 572 1051 1829 2019 2811 572
Size: 479 778 190 792 1798
Fragments arranged by size:
1798 792 778 479 190
Bsp1286I G_dGCh'C
Cuts at: 572 1051 1662 1755 1829 2019 2081 2811 572
Size: 479 611 93 74 190 62 730 1798
Fragments arranged by size:
1798 730 611 479 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4037
BstYI r'GATC_y
Cuts at: 1 1062 1529 1552 1887 2133 3145 3231 3243
Size: 1061 467 23 335 246 1012 86 12
Cuts at: 3243 3589 1
Size: 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 595 595
Size: 4037
BtgI C'CryG_G
Cuts at: 107 113 458 842 107
Size: 6 345 384 3302
Fragments arranged by size:
3302 384 345 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 137 572 1280 137
Size: 435 708 2894
Fragments arranged by size:
2894 708 435
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 110 428 1622 1796 2187 2214 3559 110
Size: 318 1194 174 391 27 1345 588
Fragments arranged by size:
1345 1194 588 391 318 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1862 2294 3041 3309 781
Size: 1081 432 747 268 1509
Fragments arranged by size:
1509 1081 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1176 1176
Size: 4037
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 1471 1719 2741 3720 1471
Size: 248 1022 979 1788
Fragments arranged by size:
1788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 1879 1909 1911 1941 227
Size: 32 1620 30 2 30 2323
Fragments arranged by size:
2323 1620 32 30 30 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 112 430 1798 112
Size: 318 1368 2351
Fragments arranged by size:
2351 1368 318
MslI CAynn'nnrTG
Cuts at: 112 130 196 577 608 874 880 2026 2153
Size: 18 66 381 31 266 6 1146 127
Cuts at: 2153 112
Size: 1996
Fragments arranged by size:
1996 1146 381 266 127 66 31 18
6
MspA1I CmG'CkG
Cuts at: 1148 1462 1822 2835 3080 1148
Size: 314 360 1013 245 2105
Fragments arranged by size:
2105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 113 458 107
Size: 6 345 3686
Fragments arranged by size:
3686 345 6
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 827 1090 1590 2497 827
Size: 263 500 907 2367
Fragments arranged by size:
2367 907 500 263
PciI A'CATG_T
Cuts at: 823 2493 823
Size: 1670 2367
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PstI C_TGCA'G
Cuts at: 1084 1769 1084
Size: 685 3352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 1080 1765 2758 2949 33
Size: 1047 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 808 1192 2107 2599 2861 808
Size: 384 915 492 262 1984
Fragments arranged by size:
1984 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StuI AGG'CCT
Cuts at: 814 814
Size: 4037
StyI C'CwwG_G
Cuts at: 107 113 419 458 1171 1525 107
Size: 6 306 39 713 354 2619
Fragments arranged by size:
2619 713 354 306 39 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 966 1620 1753 2212 2421 966
Size: 654 133 459 209 2582
Fragments arranged by size:
2582 654 459 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 121 1834 121
Size: 1713 2324
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 333 333
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI