Restriction map for
pF1KE0882
(Circular) (Six-base) MAPSORT of: pF1KE0882.seq Check: 8897 from: 1 to: 4067
pF1KE0882 4067 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 899 958 1055 1105 899
Size: 59 97 50 3861
Fragments arranged by size:
3861 97 59 50
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 638 3907 638
Size: 3269 798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 932 1892 2324 3071 3339 797
Size: 135 960 432 747 268 1525
Fragments arranged by size:
1525 960 747 432 268 135
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 357 820 1372 2939 357
Size: 463 552 1567 1485
Fragments arranged by size:
1567 1485 552 463
ApaI G_GGCC'C
Cuts at: 754 754
Size: 4067
ApaLI G'TGCA_C
Cuts at: 1022 2837 1022
Size: 1815 2252
ApoI r'AATT_y
Cuts at: 1071 3420 3676 1071
Size: 2349 256 1462
Fragments arranged by size:
2349 1462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 165 1087 165
Size: 922 3145
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 437 470 437
Size: 33 4034
BamHI G'GATC_C
Cuts at: 850 1092 850
Size: 242 3825
BanI G'GyrC_C
Cuts at: 883 1083 1497 1745 1780 883
Size: 200 414 248 35 3170
Fragments arranged by size:
3170 414 248 200 35
BanII G_rGCy'C
Cuts at: 754 1081 2111 754
Size: 327 1030 2710
Fragments arranged by size:
2710 1030 327
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BfrBI ATG'CAT
Cuts at: 430 430
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 754 762 1026 1692 1785 2841 754
Size: 8 264 666 93 1056 1980
Fragments arranged by size:
1980 1056 666 264 93 8
BmrI ACTGGGnnnn_n'
Cuts at: 241 345 629 1279 1360 1695 3839 241
Size: 104 284 650 81 335 2144 469
Fragments arranged by size:
2144 650 469 335 284 104 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 255
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 1243 2122 2614 2912 573
Size: 670 879 492 298 1728
Fragments arranged by size:
1728 879 670 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 375 3771 375
Size: 3396 671
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 141 1256 1777 2455 2729 2876 3287 141
Size: 1115 521 678 274 147 411 921
Fragments arranged by size:
1115 921 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 4067
BseYI C'CCAG_C
Cuts at: 557 628 747 1492 2250 2827 3323 557
Size: 71 119 745 758 577 496 1301
Fragments arranged by size:
1301 758 745 577 496 119 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 487 559 262
Size: 225 72 3770
Fragments arranged by size:
3770 225 72
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1026 1081 1859 2049 2841 1026
Size: 55 778 190 792 2252
Fragments arranged by size:
2252 792 778 190 55
Bsp1286I G_dGCh'C
Cuts at: 754 762 1026 1081 1692 1785 1859 2049 2111
Size: 8 264 55 611 93 74 190 62
Cuts at: 2111 2841 754
Size: 730 1980
Fragments arranged by size:
1980 730 611 264 190 93 74 62
55 8
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 455 1979 455
Size: 1524 2543
BsrFI r'CCGG_y
Cuts at: 668 2065 2246 2455 668
Size: 1397 181 209 2280
Fragments arranged by size:
2280 1397 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 231 231
Size: 4067
BstYI r'GATC_y
Cuts at: 1 850 1092 1559 1582 1917 2163 3175 3261
Size: 849 242 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 849 467 449 346 335 246 242
86 23 12
BstZ17I GTA'TAC
Cuts at: 959 959
Size: 4067
BtgI C'CryG_G
Cuts at: 863 863
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 343 1310 343
Size: 967 3100
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 485 670 716 977 1652 1826 2217 2244 3589
Size: 185 46 261 675 174 391 27 1345
Cuts at: 3589 485
Size: 963
Fragments arranged by size:
1345 963 675 391 261 185 174 46
27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 199 2090 2300 199
Size: 1891 210 1966
Fragments arranged by size:
1966 1891 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 797 932 1892 2324 3071 3339 255
Size: 542 135 960 432 747 268 983
Fragments arranged by size:
983 960 747 542 432 268 135
EcoO109I rG'GnC_Cy
Cuts at: 751 1206 751
Size: 455 3612
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 842 874 1909 1939 1941 1971 161
Size: 32 649 32 1035 30 2 30 2257
Fragments arranged by size:
2257 1035 649 32 32 30 30 2
HincII GTy'rAC
Cuts at: 642 1106 642
Size: 464 3603
HpaI GTT'AAC
Cuts at: 642 642
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 487 718 979 1828 487
Size: 231 261 849 2726
Fragments arranged by size:
2726 849 261 231
MslI CAynn'nnrTG
Cuts at: 707 2056 2183 707
Size: 1349 127 2591
Fragments arranged by size:
2591 1349 127
MspA1I CmG'CkG
Cuts at: 401 817 1178 1492 1852 2865 3110 401
Size: 416 361 314 360 1013 245 1358
Fragments arranged by size:
1358 1013 416 361 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 863 863
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NsiI A_TGCA'T
Cuts at: 432 432
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PflMI CCAn_nnn'nTGG
Cuts at: 357 357
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PspOMI G'GGCC_C
Cuts at: 750 750
Size: 4067
PstI C_TGCA'G
Cuts at: 1033 1114 1799 1033
Size: 81 685 3301
Fragments arranged by size:
3301 685 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 817 1492 1852 817
Size: 675 360 3032
Fragments arranged by size:
3032 675 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 1029 1110 1795 2788 2979 33
Size: 996 81 685 993 191 1121
Fragments arranged by size:
1121 996 993 685 191 81
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 165 552 1222 2137 2629 2891 165
Size: 387 670 915 492 262 1341
Fragments arranged by size:
1341 915 670 492 387 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 156 863 1201 1555 156
Size: 707 338 354 2668
Fragments arranged by size:
2668 707 354 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 549 1035 1650 1783 2242 2451 300
Size: 249 486 615 133 459 209 1916
Fragments arranged by size:
1916 615 486 459 249 209 133
TatI w'GTAC_w
Cuts at: 768 3582 768
Size: 2814 1253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 588 972 1562 588
Size: 384 590 3093
Fragments arranged by size:
3093 590 384
XhoI C'TCGA_G
Cuts at: 165 165
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI