Restriction map for
pF1KE0886
(Circular) (Six-base) MAPSORT of: pF1KE0886.seq Check: 9374 from: 1 to: 4082
pF1KE0886 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1907 2339 3086 3354 709
Size: 1198 432 747 268 1437
Fragments arranged by size:
1437 1198 747 432 268
AflIII A'CryG_T
Cuts at: 1434 2538 3515 1434
Size: 1104 977 2001
Fragments arranged by size:
2001 1104 977
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AleI CACnn'nnGTG
Cuts at: 280 973 280
Size: 693 3389
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 710 1102 710
Size: 392 3690
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 547 749 1035 1096 2126 547
Size: 202 286 61 1030 2503
Fragments arranged by size:
2503 1030 286 202 61
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 1707 1800 2856 1707
Size: 93 1056 2933
Fragments arranged by size:
2933 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1258 2137 2629 2927 796
Size: 462 879 492 298 1951
Fragments arranged by size:
1951 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 3786 910
Size: 2876 1206
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 733 1596 3633 6
Size: 727 863 2037 455
Fragments arranged by size:
2037 863 727 455
BsaHI Gr'CG_yC
Cuts at: 1513 1761 2463 3959 1513
Size: 248 702 1496 1636
Fragments arranged by size:
1636 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 792 226
Size: 566 3516
BseYI C'CCAG_C
Cuts at: 1507 2265 2842 3338 1507
Size: 758 577 496 2251
Fragments arranged by size:
2251 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 662 508
Size: 154 3928
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 547 749 1035 1096 1707 1800 1874 2064 2126
Size: 202 286 61 611 93 74 190 62
Cuts at: 2126 2856 547
Size: 730 1773
Fragments arranged by size:
1773 730 611 286 202 190 93 74
62 61
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 539 3258 539
Size: 2719 1363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BsrGI T'GTAC_A
Cuts at: 965 965
Size: 4082
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 360 1459 360
Size: 1099 2983
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstEII G'GTnAC_C
Cuts at: 975 975
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 4082
BstYI r'GATC_y
Cuts at: 1 432 1107 1574 1597 1932 2178 3190 3276
Size: 431 675 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 675 467 449 431 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1009 1009
Size: 4082
BtgI C'CryG_G
Cuts at: 458 563 458
Size: 105 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 203 455 479 623 1667 1841 2232 2259 3604
Size: 252 24 144 1044 174 391 27 1345
Cuts at: 3604 203
Size: 681
Fragments arranged by size:
1345 1044 681 391 252 174 144 27
24
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1907 2339 3086 3354 709
Size: 1198 432 747 268 1437
Fragments arranged by size:
1437 1198 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 782 816 1221 782
Size: 34 405 3643
Fragments arranged by size:
3643 405 34
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 574 1516 1764 2786 3765 574
Size: 942 248 1022 979 891
Fragments arranged by size:
1022 979 942 891 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1924 1954 1956 1986 1924
Size: 30 2 30 4020
Fragments arranged by size:
4020 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 189 2753 2937 189
Size: 2564 184 1334
Fragments arranged by size:
2564 1334 184
MscI TGG'CCA
Cuts at: 205 457 481 625 1843 205
Size: 252 24 144 1218 2444
Fragments arranged by size:
2444 1218 252 144 24
MslI CAynn'nnrTG
Cuts at: 280 952 958 973 2071 2198 280
Size: 672 6 15 1098 127 2164
Fragments arranged by size:
2164 1098 672 127 15 6
MspA1I CmG'CkG
Cuts at: 1193 1507 1867 2880 3125 1193
Size: 314 360 1013 245 2150
Fragments arranged by size:
2150 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 458 458
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 363 1135 1635 2542 363
Size: 772 500 907 1903
Fragments arranged by size:
1903 907 772 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PpuMI rG'GwC_Cy
Cuts at: 782 816 782
Size: 34 4048
PspXI vC'TCGA_Gb
Cuts at: 710 710
Size: 4082
PstI C_TGCA'G
Cuts at: 356 1129 1814 356
Size: 773 685 2624
Fragments arranged by size:
2624 773 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 1507 1867 1507
Size: 360 3722
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 352 1002 1125 1810 2803 2994 33
Size: 319 650 123 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 319 191 123
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 710 811 1237 2152 2644 2906 710
Size: 101 426 915 492 262 1886
Fragments arranged by size:
1886 915 492 426 262 101
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StuI AGG'CCT
Cuts at: 844 844
Size: 4082
StyI C'CwwG_G
Cuts at: 458 777 1216 1570 458
Size: 319 439 354 2970
Fragments arranged by size:
2970 439 354 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 714 1665 1798 2257 2466 570
Size: 144 951 133 459 209 2186
Fragments arranged by size:
2186 951 459 209 144 133
TatI w'GTAC_w
Cuts at: 410 965 3597 410
Size: 555 2632 895
Fragments arranged by size:
2632 895 555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 949 1879 949
Size: 930 3152
XbaI T'CTAG_A
Cuts at: 435 1113 435
Size: 678 3404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XhoI C'TCGA_G
Cuts at: 710 710
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI