Restriction map for
pF1KE0887
(Circular) (Six-base) MAPSORT of: pF1KE0887.seq Check: 2727 from: 1 to: 4166
pF1KE0887 4166 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 949 949
Size: 4166
AccI GT'mk_AC
Cuts at: 513 1204 513
Size: 691 3475
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 881 4006 881
Size: 3125 1041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1991 2423 3170 3438 919
Size: 1072 432 747 268 1647
Fragments arranged by size:
1647 1072 747 432 268
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 450 450
Size: 4166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 703 735 703
Size: 32 4134
AlwNI CAG_nnn'CTG
Cuts at: 1471 3038 1471
Size: 1567 2599
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 1148 1170 3519 3775 1148
Size: 22 2349 256 1539
Fragments arranged by size:
2349 1539 256 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 1186 1186
Size: 4166
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 1182 1596 1844 1879 1182
Size: 414 248 35 3469
Fragments arranged by size:
3469 414 248 35
BanII G_rGCy'C
Cuts at: 634 641 1180 2210 634
Size: 7 539 1030 2590
Fragments arranged by size:
2590 1030 539 7
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbsI GAAGACnn'nnnn_
Cuts at: 1109 1109
Size: 4166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 667 633
Size: 34 4132
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 623 1686 623
Size: 1063 3103
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 1791 1884 2940 1791
Size: 93 1056 3017
Fragments arranged by size:
3017 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 836 1378 1459 1794 3938 836
Size: 542 81 335 2144 1064
Fragments arranged by size:
2144 1064 542 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 571 304
Size: 267 3899
Bpu10I CC'TnA_GC
Cuts at: 914 914
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 838 969 3870 838
Size: 131 2901 1134
Fragments arranged by size:
2901 1134 131
BsaAI yAC'GTr
Cuts at: 591 1519 591
Size: 928 3238
BsaBI GATnn'nnATC
Cuts at: 6 820 1680 3717 6
Size: 814 860 2037 455
Fragments arranged by size:
2037 860 814 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 340 301
Size: 39 4127
BseYI C'CCAG_C
Cuts at: 1591 2349 2926 3422 1591
Size: 758 577 496 2335
Fragments arranged by size:
2335 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 771
Size: 4166
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
BsmI GAATG_Cn'
Cuts at: 556 681 556
Size: 125 4041
Bsp1286I G_dGCh'C
Cuts at: 634 641 1180 1791 1884 1958 2148 2210 2940
Size: 7 539 611 93 74 190 62 730
Cuts at: 2940 634
Size: 1860
Fragments arranged by size:
1860 730 611 539 190 93 74 62
7
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 626 671 3342 626
Size: 45 2671 1450
Fragments arranged by size:
2671 1450 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1216 1430 1732 2113 949
Size: 267 214 302 381 3002
Fragments arranged by size:
3002 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BsrGI T'GTAC_A
Cuts at: 1052 1052
Size: 4166
BssSI C'ACGA_G
Cuts at: 652 2437 2795 652
Size: 1785 358 2023
Fragments arranged by size:
2023 1785 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstXI CCAn_nnnn'nTGG
Cuts at: 810 810
Size: 4166
BstYI r'GATC_y
Cuts at: 1 479 740 902 1191 1658 1681 2016 2262
Size: 478 261 162 289 467 23 335 246
Cuts at: 2262 3274 3360 3372 3718 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 478 467 449 346 335 289 261
246 162 86 23 12
BtgI C'CryG_G
Cuts at: 959 959
Size: 4166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 593 1751 1925 2316 2343 3688 593
Size: 1158 174 391 27 1345 1071
Fragments arranged by size:
1345 1158 1071 391 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 976 2189 2399 976
Size: 1213 210 2743
Fragments arranged by size:
2743 1213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 571 919 1991 2423 3170 3438 304
Size: 267 348 1072 432 747 268 1032
Fragments arranged by size:
1072 1032 747 432 348 268 267
EcoO109I rG'GnC_Cy
Cuts at: 1305 1305
Size: 4166
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 318 2185 318
Size: 1867 2299
FspI TGC'GCA
Cuts at: 729 1947 729
Size: 1218 2948
HaeII r_GCGC'y
Cuts at: 1600 1848 2870 3849 1600
Size: 248 1022 979 1917
Fragments arranged by size:
1917 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 471 503 2008 2038 2040 2070 102
Size: 32 337 32 1505 30 2 30 2198
Fragments arranged by size:
2198 1505 337 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 138 2837 3021 138
Size: 2699 184 1283
Fragments arranged by size:
2699 1283 184
MscI TGG'CCA
Cuts at: 595 1927 595
Size: 1332 2834
MslI CAynn'nnrTG
Cuts at: 850 2155 2282 850
Size: 1305 127 2734
Fragments arranged by size:
2734 1305 127
MspA1I CmG'CkG
Cuts at: 282 1277 1591 1951 2964 3209 282
Size: 995 314 360 1013 245 1239
Fragments arranged by size:
1239 1013 995 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NdeI CA'TA_TG
Cuts at: 598 598
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PasI CC'CwG_GG
Cuts at: 634 634
Size: 4166
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PfoI T'CCnGG_A
Cuts at: 664 664
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 871 903 3305 3337 871
Size: 32 2402 32 1700
Fragments arranged by size:
2402 1700 32 32
PstI C_TGCA'G
Cuts at: 946 1213 1898 946
Size: 267 685 3214
Fragments arranged by size:
3214 685 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 282 1591 1951 282
Size: 1309 360 2497
Fragments arranged by size:
2497 1309 360
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 213 517 942 1089 1209 1894 2887 3078
Size: 180 304 425 147 120 685 993 191
Cuts at: 3078 33
Size: 1121
Fragments arranged by size:
1121 993 685 425 304 191 180 147
120
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
SspI AAT'ATT
Cuts at: 145 145
Size: 4166
StyI C'CwwG_G
Cuts at: 1300 1654 1300
Size: 354 3812
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 620 1749 1882 2341 2550 205
Size: 415 1129 133 459 209 1821
Fragments arranged by size:
1821 1129 459 415 209 133
TatI w'GTAC_w
Cuts at: 323 398 1052 3681 323
Size: 75 654 2629 808
Fragments arranged by size:
2629 808 654 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
XmnI GAAnn'nnTTC
Cuts at: 883 883
Size: 4166
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI