Restriction map for
pF1KE0889
(Circular) (Six-base) MAPSORT of: pF1KE0889.seq Check: 9302 from: 1 to: 4079
pF1KE0889 4079 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1904 2336 3083 3351 914
Size: 990 432 747 268 1642
Fragments arranged by size:
1642 990 747 432 268
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 1384 2951 1384
Size: 1567 2512
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 1099 1099
Size: 4079
BamHI G'GATC_C
Cuts at: 832 1104 832
Size: 272 3807
BanI G'GyrC_C
Cuts at: 652 1095 1509 1757 1792 652
Size: 443 414 248 35 2939
Fragments arranged by size:
2939 443 414 248 35
BanII G_rGCy'C
Cuts at: 115 1008 1093 2123 115
Size: 893 85 1030 2071
Fragments arranged by size:
2071 1030 893 85
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BfrBI ATG'CAT
Cuts at: 398 398
Size: 4079
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 657 1704 1797 2853 657
Size: 1047 93 1056 1883
Fragments arranged by size:
1883 1056 1047 93
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1372 1707 3851 1291
Size: 81 335 2144 1519
Fragments arranged by size:
2144 1519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 4079
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1041 1255 2134 2626 2924 646
Size: 395 214 879 492 298 1801
Fragments arranged by size:
1801 879 492 395 298 214
BsaI GGTCTCn'nnnn_
Cuts at: 883 3783 883
Size: 2900 1179
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 580 136
Size: 444 3635
BseYI C'CCAG_C
Cuts at: 225 544 599 1504 2262 2839 3335 225
Size: 319 55 905 758 577 496 969
Fragments arranged by size:
969 905 758 577 496 319 55
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 576 1093 1871 2061 2853 576
Size: 517 778 190 792 1802
Fragments arranged by size:
1802 792 778 517 190
Bsp1286I G_dGCh'C
Cuts at: 115 576 657 1008 1093 1704 1797 1871 2061
Size: 461 81 351 85 611 93 74 190
Cuts at: 2061 2123 2853 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 461 351 190 93 85
81 74 62
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 512 3255 512
Size: 2743 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1129 1343 1645 2026 242
Size: 887 214 302 381 2295
Fragments arranged by size:
2295 887 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4079
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstEII G'GTnAC_C
Cuts at: 774 774
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 4079
BstYI r'GATC_y
Cuts at: 1 832 1104 1571 1594 1929 2175 3187 3273
Size: 831 272 467 23 335 246 1012 86
Cuts at: 3273 3285 3631 1
Size: 12 346 449
Fragments arranged by size:
1012 831 467 449 346 335 272 246
86 23 12
BtgI C'CryG_G
Cuts at: 218 218
Size: 4079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 543 2029 2210 2373 543
Size: 1486 181 163 2249
Fragments arranged by size:
2249 1486 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1322 1043
Size: 279 3800
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 166 479 1664 1838 2229 2256 3601 166
Size: 313 1185 174 391 27 1345 644
Fragments arranged by size:
1345 1185 644 391 313 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 914 1904 2336 3083 3351 769
Size: 145 990 432 747 268 1497
Fragments arranged by size:
1497 990 747 432 268 145
EcoO109I rG'GnC_Cy
Cuts at: 548 1218 548
Size: 670 3409
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 4079
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 1513 1761 2783 3762 1513
Size: 248 1022 979 1830
Fragments arranged by size:
1830 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 824 856 1921 1951 1953 1983 824
Size: 32 1065 30 2 30 2920
Fragments arranged by size:
2920 1065 32 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2750 2934 2750
Size: 184 3895
MscI TGG'CCA
Cuts at: 481 1840 481
Size: 1359 2720
MslI CAynn'nnrTG
Cuts at: 280 457 511 538 741 955 2068 2195 280
Size: 177 54 27 203 214 1113 127 2164
Fragments arranged by size:
2164 1113 214 203 177 127 54 27
MspA1I CmG'CkG
Cuts at: 1190 1504 1864 2877 3122 1190
Size: 314 360 1013 245 2147
Fragments arranged by size:
2147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NdeI CA'TA_TG
Cuts at: 465 808 465
Size: 343 3736
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NsiI A_TGCA'T
Cuts at: 400 400
Size: 4079
NspI r_CATG'y
Cuts at: 210 363 1132 1632 2539 210
Size: 153 769 500 907 1750
Fragments arranged by size:
1750 907 769 500 153
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PpuMI rG'GwC_Cy
Cuts at: 548 548
Size: 4079
PstI C_TGCA'G
Cuts at: 1126 1811 1126
Size: 685 3394
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 1504 1864 1504
Size: 360 3719
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 1122 1807 2800 2991 33
Size: 1089 685 993 191 1121
Fragments arranged by size:
1121 1089 993 685 191
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 661 1020 1234 2149 2641 2903 661
Size: 359 214 915 492 262 1837
Fragments arranged by size:
1837 915 492 359 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StyI C'CwwG_G
Cuts at: 648 800 1213 1567 648
Size: 152 413 354 3160
Fragments arranged by size:
3160 413 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 284 3594 284
Size: 3310 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1574 1574
Size: 4079
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
XmnI GAAnn'nnTTC
Cuts at: 967 967
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI