Restriction map for
pF1KE0893
(Circular) (Six-base) MAPSORT of: pF1KE0893.seq Check: 1399 from: 1 to: 4154
pF1KE0893 4154 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2411 3158 3426 1979
Size: 432 747 268 2707
Fragments arranged by size:
2707 747 432 268
AflII C'TTAA_G
Cuts at: 127 127
Size: 4154
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AleI CACnn'nnGTG
Cuts at: 1057 1057
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 468 1139 1158 3507 3763 468
Size: 671 19 2349 256 859
Fragments arranged by size:
2349 859 671 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 1174 1174
Size: 4154
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 1170 1584 1832 1867 1170
Size: 414 248 35 3457
Fragments arranged by size:
3457 414 248 35
BanII G_rGCy'C
Cuts at: 1168 2198 1168
Size: 1030 3124
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglI GCCn_nnn'nGGC
Cuts at: 571 571
Size: 4154
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 1779 1872 2928 1779
Size: 93 1056 3005
Fragments arranged by size:
3005 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 783
Size: 4154
Bpu10I CC'TnA_GC
Cuts at: 281 828 281
Size: 547 3607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 2209 2701 2999 1330
Size: 879 492 298 2485
Fragments arranged by size:
2485 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 817 3858 817
Size: 3041 1113
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 1585 1833 2535 4031 1585
Size: 248 702 1496 1708
Fragments arranged by size:
1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 655 685 781 811 170
Size: 30 455 30 96 30 3513
Fragments arranged by size:
3513 455 96 30 30 30
BseYI C'CCAG_C
Cuts at: 761 1579 2337 2914 3410 761
Size: 818 758 577 496 1505
Fragments arranged by size:
1505 818 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 582 1696 1742 2950 104
Size: 478 1114 46 1208 1308
Fragments arranged by size:
1308 1208 1114 478 46
BsiHKAI G_wGCw'C
Cuts at: 350 896 1168 1946 2136 2928 350
Size: 546 272 778 190 792 1576
Fragments arranged by size:
1576 792 778 546 272 190
Bsp1286I G_dGCh'C
Cuts at: 350 896 1168 1779 1872 1946 2136 2198 2928
Size: 546 272 611 93 74 190 62 730
Cuts at: 2928 350
Size: 1576
Fragments arranged by size:
1576 730 611 546 272 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 566 881 1204 1418 1720 2101 332
Size: 234 315 323 214 302 381 2385
Fragments arranged by size:
2385 381 323 315 302 234 214
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 2066 636
Size: 1430 2724
BsrFI r'CCGG_y
Cuts at: 581 2152 2333 2542 581
Size: 1571 181 209 2193
Fragments arranged by size:
2193 1571 209 181
BsrGI T'GTAC_A
Cuts at: 1049 1049
Size: 4154
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 642 1531 642
Size: 889 3265
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 216 807 216
Size: 591 3563
BstYI r'GATC_y
Cuts at: 1 1179 1646 1669 2004 2250 3262 3348 3360
Size: 1178 467 23 335 246 1012 86 12
Cuts at: 3360 3706 1
Size: 346 449
Fragments arranged by size:
1178 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 246 1093 246
Size: 847 3307
BtgI C'CryG_G
Cuts at: 631 631
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 178 953 1397 178
Size: 775 444 2935
Fragments arranged by size:
2935 775 444
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 359 572 590 1739 1913 2304 2331 3676 359
Size: 213 18 1149 174 391 27 1345 837
Fragments arranged by size:
1345 1149 837 391 213 174 27 18
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 276 1105 2177 2387 276
Size: 829 1072 210 2043
Fragments arranged by size:
2043 1072 829 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1166 1166
Size: 4154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 1979 2411 3158 3426 783
Size: 1196 432 747 268 1511
Fragments arranged by size:
1511 1196 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1001 1293 1001
Size: 292 3862
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
EcoRV GAT'ATC
Cuts at: 429 429
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 781 813 1996 2026 2028 2058 781
Size: 32 1183 30 2 30 2877
Fragments arranged by size:
2877 1183 32 30 30 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
HindIII A'AGCT_T
Cuts at: 130 130
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 2825 3009 299
Size: 2526 184 1444
Fragments arranged by size:
2526 1444 184
MscI TGG'CCA
Cuts at: 574 592 1915 574
Size: 18 1323 2813
Fragments arranged by size:
2813 1323 18
MslI CAynn'nnrTG
Cuts at: 391 614 1045 1057 2143 2270 391
Size: 223 431 12 1086 127 2275
Fragments arranged by size:
2275 1086 431 223 127 12
MspA1I CmG'CkG
Cuts at: 1265 1579 1939 2952 3197 1265
Size: 314 360 1013 245 2222
Fragments arranged by size:
2222 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PflMI CCAn_nnn'nTGG
Cuts at: 810 920 810
Size: 110 4044
PfoI T'CCnGG_A
Cuts at: 199 199
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PstI C_TGCA'G
Cuts at: 290 912 960 1201 1886 290
Size: 622 48 241 685 2558
Fragments arranged by size:
2558 685 622 241 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 1579 1939 1579
Size: 360 3794
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 1168 1168
Size: 4154
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 231 286 908 956 1086 1197 1882 2875
Size: 198 55 622 48 130 111 685 993
Cuts at: 2875 3066 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 622 198 191 130 111
55 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 571 571
Size: 4154
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 127 1309 2224 2716 2978 127
Size: 1182 915 492 262 1303
Fragments arranged by size:
1303 1182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StuI AGG'CCT
Cuts at: 928 928
Size: 4154
StyI C'CwwG_G
Cuts at: 1288 1642 1288
Size: 354 3800
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 775 1080 1737 1870 2329 2538 775
Size: 305 657 133 459 209 2391
Fragments arranged by size:
2391 657 459 305 209 133
TatI w'GTAC_w
Cuts at: 395 521 1013 1049 3669 395
Size: 126 492 36 2620 880
Fragments arranged by size:
2620 880 492 126 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 667 699 3671 3703 170
Size: 32 465 32 2972 32 621
Fragments arranged by size:
2972 621 465 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 582 807 1017 1649 582
Size: 225 210 632 3087
Fragments arranged by size:
3087 632 225 210
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
XmnI GAAnn'nnTTC
Cuts at: 928 1078 928
Size: 150 4004
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI