Restriction map for
pF1KE0896
(Circular) (Six-base) MAPSORT of: pF1KE0896.seq Check: 1428 from: 1 to: 4103
pF1KE0896 4103 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1928 2360 3107 3375 733
Size: 1195 432 747 268 1461
Fragments arranged by size:
1461 1195 747 432 268
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 274 274
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 1408 2975 1408
Size: 1567 2536
ApaLI G'TGCA_C
Cuts at: 581 2873 581
Size: 2292 1811
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 722 755 722
Size: 33 4070
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 1119 1533 1781 1816 1119
Size: 414 248 35 3406
Fragments arranged by size:
3406 414 248 35
BanII G_rGCy'C
Cuts at: 1117 2147 1117
Size: 1030 3073
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 795 795
Size: 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 752 1623 752
Size: 871 3232
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 585 669 1728 1821 2877 585
Size: 84 1059 93 1056 1811
Fragments arranged by size:
1811 1059 1056 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1279 2158 2650 2948 1053
Size: 226 879 492 298 2208
Fragments arranged by size:
2208 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 923 3807 923
Size: 2884 1219
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 754 1617 3654 6
Size: 748 863 2037 455
Fragments arranged by size:
2037 863 748 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 238
Size: 4103
BseYI C'CCAG_C
Cuts at: 572 591 602 1528 2286 2863 3359 572
Size: 19 11 926 758 577 496 1316
Fragments arranged by size:
1316 926 758 577 496 19 11
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 585 708 1117 1895 2085 2877 585
Size: 123 409 778 190 792 1811
Fragments arranged by size:
1811 792 778 409 190 123
Bsp1286I G_dGCh'C
Cuts at: 585 669 708 1117 1728 1821 1895 2085 2147
Size: 84 39 409 611 93 74 190 62
Cuts at: 2147 2877 585
Size: 730 1811
Fragments arranged by size:
1811 730 611 409 190 93 84 74
62 39
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 1153 1367 1669 2050 293
Size: 860 214 302 381 2346
Fragments arranged by size:
2346 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstEII G'GTnAC_C
Cuts at: 267 267
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 402 402
Size: 4103
BstYI r'GATC_y
Cuts at: 1 1128 1595 1618 1953 2199 3211 3297 3309
Size: 1127 467 23 335 246 1012 86 12
Cuts at: 3309 3655 1
Size: 346 449
Fragments arranged by size:
1127 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 272 761 107
Size: 165 489 3449
Fragments arranged by size:
3449 489 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 1346
Size: 4103
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 217 299 347 404 530 1688 1862 2253 2280
Size: 82 48 57 126 1158 174 391 27
Cuts at: 2280 3625 217
Size: 1345 695
Fragments arranged by size:
1345 1158 695 391 174 126 82 57
48 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 239 913 2126 2336 239
Size: 674 1213 210 2006
Fragments arranged by size:
2006 1213 674 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2311 2621 2767 3913 2311
Size: 310 146 1146 2501
Fragments arranged by size:
2501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 872 1928 2360 3107 3375 733
Size: 139 1056 432 747 268 1461
Fragments arranged by size:
1461 1056 747 432 268 139
EcoO109I rG'GnC_Cy
Cuts at: 1242 1242
Size: 4103
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 127 1884 127
Size: 1757 2346
HaeII r_GCGC'y
Cuts at: 1537 1785 2807 3786 1537
Size: 248 1022 979 1854
Fragments arranged by size:
1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1945 1975 1977 2007 1945
Size: 30 2 30 4041
Fragments arranged by size:
4041 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
HindIII A'AGCT_T
Cuts at: 715 715
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 219 301 349 406 532 1864 219
Size: 82 48 57 126 1332 2458
Fragments arranged by size:
2458 1332 126 82 57 48
MslI CAynn'nnrTG
Cuts at: 274 562 760 2092 2219 274
Size: 288 198 1332 127 2158
Fragments arranged by size:
2158 1332 288 198 127
MspA1I CmG'CkG
Cuts at: 1214 1528 1888 2901 3146 1214
Size: 314 360 1013 245 2171
Fragments arranged by size:
2171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 107 272 761 107
Size: 165 489 3449
Fragments arranged by size:
3449 489 165
NdeI CA'TA_TG
Cuts at: 516 516
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NspI r_CATG'y
Cuts at: 261 1156 1656 2563 261
Size: 895 500 907 1801
Fragments arranged by size:
1801 907 895 500
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PfoI T'CCnGG_A
Cuts at: 849 849
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PstI C_TGCA'G
Cuts at: 1150 1835 1150
Size: 685 3418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 913 2126 2336 913
Size: 1213 210 2680
Fragments arranged by size:
2680 1213 210
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SexAI A'CCwGG_T
Cuts at: 263 263
Size: 4103
SfcI C'TryA_G
Cuts at: 33 959 1146 1831 2824 3015 33
Size: 926 187 685 993 191 1121
Fragments arranged by size:
1121 993 926 685 191 187
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 1032 1258 2173 2665 2927 1032
Size: 226 915 492 262 2208
Fragments arranged by size:
2208 915 492 262 226
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 868 868
Size: 4103
StyI C'CwwG_G
Cuts at: 107 252 272 761 1237 1591 107
Size: 145 20 489 476 354 2619
Fragments arranged by size:
2619 489 476 354 145 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 434 583 594 916 1686 1819 2278 2487 434
Size: 149 11 322 770 133 459 209 2050
Fragments arranged by size:
2050 770 459 322 209 149 133 11
TatI w'GTAC_w
Cuts at: 335 3618 335
Size: 3283 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
XmnI GAAnn'nnTTC
Cuts at: 868 868
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI