Restriction map for
pF1KE0911
(Circular) (Six-base) MAPSORT of: pF1KE0911.seq Check: 5922 from: 1 to: 3830
pF1KE0911 3830 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 332 556 332
Size: 224 3606
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 839 1655 2087 2834 3102 773
Size: 66 816 432 747 268 1501
Fragments arranged by size:
1501 816 747 432 268 66
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2702 1135
Size: 1567 2263
ApaI G_GGCC'C
Cuts at: 273 273
Size: 3830
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 778 786 834 3183 3439 778
Size: 8 48 2349 256 1169
Fragments arranged by size:
2349 1169 256 48 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 850 850
Size: 3830
BamHI G'GATC_C
Cuts at: 596 855 596
Size: 259 3571
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 273 647 844 1874 273
Size: 374 197 1030 2229
Fragments arranged by size:
2229 1030 374 197
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BbsI GAAGACnn'nnnn_
Cuts at: 827 827
Size: 3830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3796
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 434 434
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 170 273 1455 1548 2604 170
Size: 103 1182 93 1056 1396
Fragments arranged by size:
1396 1182 1056 103 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 175 259 160
Size: 15 84 3731
Fragments arranged by size:
3731 84 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 344 513 1183 344
Size: 169 670 2991
Fragments arranged by size:
2991 670 169
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 308 1261 1509 2211 3707 308
Size: 953 248 702 1496 431
Fragments arranged by size:
1496 953 702 431 248
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 376
Size: 3830
BseYI C'CCAG_C
Cuts at: 432 1255 2013 2590 3086 432
Size: 823 758 577 496 1176
Fragments arranged by size:
1176 823 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 241 246 721 160
Size: 81 5 475 3269
Fragments arranged by size:
3269 475 81 5
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2626 104
Size: 1268 46 1208 1308
Fragments arranged by size:
1308 1268 1208 46
BsiHKAI G_wGCw'C
Cuts at: 844 1622 1812 2604 844
Size: 778 190 792 2070
Fragments arranged by size:
2070 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 315 315
Size: 3830
Bsp1286I G_dGCh'C
Cuts at: 170 273 647 844 1455 1548 1622 1812 1874
Size: 103 374 197 611 93 74 190 62
Cuts at: 1874 2604 170
Size: 730 1396
Fragments arranged by size:
1396 730 611 374 197 190 103 93
74 62
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 125 3006 125
Size: 2881 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 556 880 1094 1396 1777 332
Size: 224 324 214 302 381 2385
Fragments arranged by size:
2385 381 324 302 224 214
BsrBI CCG'CTC
Cuts at: 50 2122 2176 3660 3825 50
Size: 2072 54 1484 165 55
Fragments arranged by size:
2072 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 473 1742 473
Size: 1269 2561
BsrFI r'CCGG_y
Cuts at: 304 428 1828 2009 2218 304
Size: 124 1400 181 209 1916
Fragments arranged by size:
1916 1400 209 181 124
BssHII G'CGCG_C
Cuts at: 300 300
Size: 3830
BssSI C'ACGA_G
Cuts at: 178 2101 2459 178
Size: 1923 358 1549
Fragments arranged by size:
1923 1549 358
BstAPI GCAn_nnn'nTGC
Cuts at: 548 1207 548
Size: 659 3171
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3830
BstYI r'GATC_y
Cuts at: 1 596 855 1322 1345 1680 1926 2938 3024
Size: 595 259 467 23 335 246 1012 86
Cuts at: 3024 3036 3382 1
Size: 12 346 449
Fragments arranged by size:
1012 595 467 449 346 335 259 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 258 1073 258
Size: 815 3015
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DraIII CAC_nnn'GTG
Cuts at: 183 699 183
Size: 516 3314
DrdI GACnn_nn'nnGTC
Cuts at: 409 1536 2394 409
Size: 1127 858 1845
Fragments arranged by size:
1845 1127 858
EaeI y'GGCC_r
Cuts at: 282 1415 1589 1980 2007 3352 282
Size: 1133 174 391 27 1345 760
Fragments arranged by size:
1345 1133 760 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 175 259 773 839 1655 2087 2834 3102
Size: 15 84 514 66 816 432 747 268
Cuts at: 3102 160
Size: 888
Fragments arranged by size:
888 816 747 514 432 268 84 66
15
EcoNI CCTnn'n_nnAGG
Cuts at: 214 214
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 269 270 969 269
Size: 1 699 3130
Fragments arranged by size:
3130 699 1
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 1264 1512 2534 3513 1264
Size: 248 1022 979 1581
Fragments arranged by size:
1581 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 607 639 1672 1702 1704 1734 607
Size: 32 1033 30 2 30 2703
Fragments arranged by size:
2703 1033 32 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2685 2501
Size: 184 3646
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 1819 1946 1819
Size: 127 3703
MspA1I CmG'CkG
Cuts at: 255 288 941 1255 1615 2628 2873 255
Size: 33 653 314 360 1013 245 1212
Fragments arranged by size:
1212 1013 653 360 314 245 33
NaeI GCC'GGC
Cuts at: 306 430 2011 306
Size: 124 1581 2125
Fragments arranged by size:
2125 1581 124
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NgoMIV G'CCGG_C
Cuts at: 304 428 2009 304
Size: 124 1581 2125
Fragments arranged by size:
2125 1581 124
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PasI CC'CwG_GG
Cuts at: 114 114
Size: 3830
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PfoI T'CCnGG_A
Cuts at: 236 483 236
Size: 247 3583
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PmlI CAC'GTG
Cuts at: 344 513 344
Size: 169 3661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PspOMI G'GGCC_C
Cuts at: 269 269
Size: 3830
PstI C_TGCA'G
Cuts at: 877 1562 877
Size: 685 3145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 255 288 1255 1615 255
Size: 33 967 360 2470
Fragments arranged by size:
2470 967 360 33
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 873 1558 2551 2742 33
Size: 840 685 993 191 1121
Fragments arranged by size:
1121 993 840 685 191
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SgrAI Cr'CCGG_yG
Cuts at: 304 304
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StyI C'CwwG_G
Cuts at: 964 1318 964
Size: 354 3476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 158 3345 158
Size: 3187 643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 232 1627 232
Size: 1395 2435
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 3830
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I BtgI ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI ZraI