Restriction map for
pF1KE0914
(Circular) (Six-base) MAPSORT of: pF1KE0914.seq Check: 5603 from: 1 to: 3884
pF1KE0914 3884 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 119 3724 119
Size: 3605 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 617 648 1189 2756 617
Size: 31 541 1567 1745
Fragments arranged by size:
1745 1567 541 31
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 262 888 3237 3493 262
Size: 626 2349 256 653
Fragments arranged by size:
2349 653 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 811 904 811
Size: 93 3791
BamHI G'GATC_C
Cuts at: 909 909
Size: 3884
BanI G'GyrC_C
Cuts at: 237 900 1314 1562 1597 237
Size: 663 414 248 35 2524
Fragments arranged by size:
2524 663 414 248 35
BanII G_rGCy'C
Cuts at: 314 818 898 1928 314
Size: 504 80 1030 2270
Fragments arranged by size:
2270 1030 504 80
BbeI G_GCGC'C
Cuts at: 241 1318 1566 241
Size: 1077 248 2559
Fragments arranged by size:
2559 1077 248
BbsI GAAGACnn'nnnn_
Cuts at: 681 681
Size: 3884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3850
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 1509 1602 2658 1509
Size: 93 1056 2735
Fragments arranged by size:
2735 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 612 729 884 424
Size: 188 117 155 3424
Fragments arranged by size:
3424 188 155 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 466 3588 466
Size: 3122 762
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 238 1315 1563 2265 3761 238
Size: 1077 248 702 1496 361
Fragments arranged by size:
1496 1077 702 361 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 422 452 422
Size: 30 3854
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 443 771 847 202
Size: 3 238 328 76 3239
Fragments arranged by size:
3239 328 238 76 3
BseYI C'CCAG_C
Cuts at: 241 423 1309 2067 2644 3140 241
Size: 182 886 758 577 496 985
Fragments arranged by size:
985 886 758 577 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 3884
BsiEI CG_ry'CG
Cuts at: 104 395 527 1426 1472 2680 104
Size: 291 132 899 46 1208 1308
Fragments arranged by size:
1308 1208 899 291 132 46
BsiHKAI G_wGCw'C
Cuts at: 827 898 1676 1866 2658 827
Size: 71 778 190 792 2053
Fragments arranged by size:
2053 792 778 190 71
BsmBI CGTCTCn'nnnn_
Cuts at: 546 546
Size: 3884
Bsp1286I G_dGCh'C
Cuts at: 314 818 827 898 1509 1602 1676 1866 1928
Size: 504 9 71 611 93 74 190 62
Cuts at: 1928 2658 314
Size: 730 1540
Fragments arranged by size:
1540 730 611 504 190 93 74 71
62 9
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 647 934 1148 1450 1831 647
Size: 287 214 302 381 2700
Fragments arranged by size:
2700 381 302 287 214
BsrBI CCG'CTC
Cuts at: 50 305 515 2176 2230 3714 3879 50
Size: 255 210 1661 54 1484 165 55
Fragments arranged by size:
1661 1484 255 210 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 1796
Size: 3884
BsrFI r'CCGG_y
Cuts at: 1882 2063 2272 1882
Size: 181 209 3494
Fragments arranged by size:
3494 209 181
BssHII G'CGCG_C
Cuts at: 571 571
Size: 3884
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstEII G'GTnAC_C
Cuts at: 411 411
Size: 3884
BstYI r'GATC_y
Cuts at: 1 645 909 1376 1399 1734 1980 2992 3078
Size: 644 264 467 23 335 246 1012 86
Cuts at: 3078 3090 3436 1
Size: 12 346 449
Fragments arranged by size:
1012 644 467 449 346 335 264 246
86 23 12
BtgI C'CryG_G
Cuts at: 389 603 389
Size: 214 3670
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1127
Size: 3884
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2448 1590
Size: 858 3026
EaeI y'GGCC_r
Cuts at: 392 524 606 1469 1643 2034 2061 3406 392
Size: 132 82 863 174 391 27 1345 870
Fragments arranged by size:
1345 870 863 391 174 132 82 27
EagI C'GGCC_G
Cuts at: 392 524 1469 392
Size: 132 945 2807
Fragments arranged by size:
2807 945 132
EarI CTCTTCn'nnn_
Cuts at: 870 1907 2117 870
Size: 1037 210 2637
Fragments arranged by size:
2637 1037 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2402 2548 3694 2092
Size: 310 146 1146 2282
Fragments arranged by size:
2282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 612 729 884 1709 2141 2888 3156 424
Size: 188 117 155 825 432 747 268 1152
Fragments arranged by size:
1152 825 747 432 268 188 155 117
EcoO109I rG'GnC_Cy
Cuts at: 1023 1023
Size: 3884
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
EcoRV GAT'ATC
Cuts at: 252 252
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 241 656 752 1318 1566 2588 3567 241
Size: 415 96 566 248 1022 979 558
Fragments arranged by size:
1022 979 566 558 415 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 700 732 1726 1756 1758 1788 142
Size: 32 526 32 994 30 2 30 2238
Fragments arranged by size:
2238 994 526 32 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 237 1314 1562 237
Size: 1077 248 2559
Fragments arranged by size:
2559 1077 248
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 1873 2000 1873
Size: 127 3757
MspA1I CmG'CkG
Cuts at: 402 584 605 995 1309 1669 2682 2927 402
Size: 182 21 390 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 390 360 314 245 182 21
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 238 1315 1563 238
Size: 1077 248 2559
Fragments arranged by size:
2559 1077 248
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PstI C_TGCA'G
Cuts at: 230 644 931 1616 230
Size: 414 287 685 2498
Fragments arranged by size:
2498 685 414 287
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 402 1309 1669 402
Size: 907 360 2617
Fragments arranged by size:
2617 907 360
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SacII CC_GC'GG
Cuts at: 606 606
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 644 931 644
Size: 287 3597
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 226 640 927 1612 2605 2796 33
Size: 193 414 287 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 287 193 191
SfoI GGC'GCC
Cuts at: 239 1316 1564 239
Size: 1077 248 2559
Fragments arranged by size:
2559 1077 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 1018 1372 1018
Size: 354 3530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 430 1467 1600 2059 2268 430
Size: 1037 133 459 209 2046
Fragments arranged by size:
2046 1037 459 209 133
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 493 525 3401 3433 422
Size: 32 39 32 2876 32 873
Fragments arranged by size:
2876 873 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 789 1681 789
Size: 892 2992
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1379 1379
Size: 3884
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
XmnI GAAnn'nnTTC
Cuts at: 262 262
Size: 3884
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI