Restriction map for
pF1KE0916
(Circular) (Six-base) MAPSORT of: pF1KE0916.seq Check: 669 from: 1 to: 3974
pF1KE0916 3974 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 434 1012 434
Size: 578 3396
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 3814 3814
Size: 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1799 2231 2978 3246 1799
Size: 432 747 268 2527
Fragments arranged by size:
2527 747 432 268
AfeI AGC'GCT
Cuts at: 493 493
Size: 3974
AflIII A'CryG_T
Cuts at: 1326 2430 3407 1326
Size: 1104 977 1893
Fragments arranged by size:
1893 1104 977
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AlwNI CAG_nnn'CTG
Cuts at: 633 1279 2846 633
Size: 646 1567 1761
Fragments arranged by size:
1761 1567 646
ApaI G_GGCC'C
Cuts at: 598 598
Size: 3974
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 978 3327 3583 978
Size: 2349 256 1369
Fragments arranged by size:
2349 1369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 622 741 825 994 622
Size: 119 84 169 3602
Fragments arranged by size:
3602 169 119 84
BamHI G'GATC_C
Cuts at: 999 999
Size: 3974
BanI G'GyrC_C
Cuts at: 990 1404 1652 1687 990
Size: 414 248 35 3277
Fragments arranged by size:
3277 414 248 35
BanII G_rGCy'C
Cuts at: 581 598 988 2018 581
Size: 17 390 1030 2537
Fragments arranged by size:
2537 1030 390 17
BbeI G_GCGC'C
Cuts at: 1408 1656 1408
Size: 248 3726
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3940
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 278 1494 278
Size: 1216 2758
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 292 1086 292
Size: 794 3180
Bme1580I G_kGCm'C
Cuts at: 598 1599 1692 2748 598
Size: 1001 93 1056 1824
Fragments arranged by size:
1824 1056 1001 93
BmrI ACTGGGnnnn_n'
Cuts at: 1186 1267 1602 3746 1186
Size: 81 335 2144 1414
Fragments arranged by size:
2144 1414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 3974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 592 895 951 244
Size: 348 303 56 3267
Fragments arranged by size:
3267 348 303 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2029 2521 2819 1150
Size: 879 492 298 2305
Fragments arranged by size:
2305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 176 284 758 1327 176
Size: 108 474 569 2823
Fragments arranged by size:
2823 569 474 108
BsaBI GATnn'nnATC
Cuts at: 6 241 1488 3525 6
Size: 235 1247 2037 455
Fragments arranged by size:
2037 1247 455 235
BsaHI Gr'CG_yC
Cuts at: 1405 1653 2355 3851 1405
Size: 248 702 1496 1528
Fragments arranged by size:
1528 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 211
Size: 30 3944
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 589 363
Size: 226 3748
BseYI C'CCAG_C
Cuts at: 482 711 730 1399 2157 2734 3230 482
Size: 229 19 669 758 577 496 1226
Fragments arranged by size:
1226 758 669 577 496 229 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 296 585 156
Size: 140 289 3545
Fragments arranged by size:
3545 289 140
BsiEI CG_ry'CG
Cuts at: 104 459 1516 1562 2770 104
Size: 355 1057 46 1208 1308
Fragments arranged by size:
1308 1208 1057 355 46
BsiHKAI G_wGCw'C
Cuts at: 400 988 1766 1956 2748 400
Size: 588 778 190 792 1626
Fragments arranged by size:
1626 792 778 588 190
Bsp1286I G_dGCh'C
Cuts at: 400 581 598 988 1599 1692 1766 1956 2018
Size: 181 17 390 611 93 74 190 62
Cuts at: 2018 2748 400
Size: 730 1626
Fragments arranged by size:
1626 730 611 390 190 181 93 74
62 17
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 420 533 551 1024 1238 1540 1921 420
Size: 113 18 473 214 302 381 2473
Fragments arranged by size:
2473 473 381 302 214 113 18
BsrBI CCG'CTC
Cuts at: 50 2266 2320 3804 3969 50
Size: 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 329 1972 2153 2362 329
Size: 1643 181 209 1941
Fragments arranged by size:
1941 1643 209 181
BssHII G'CGCG_C
Cuts at: 657 657
Size: 3974
BssSI C'ACGA_G
Cuts at: 532 2245 2603 532
Size: 1713 358 1903
Fragments arranged by size:
1903 1713 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1351 1351
Size: 3974
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstYI r'GATC_y
Cuts at: 1 999 1466 1489 1824 2070 3082 3168 3180
Size: 998 467 23 335 246 1012 86 12
Cuts at: 3180 3526 1
Size: 346 449
Fragments arranged by size:
1012 998 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 888 1924 2105 2268 888
Size: 1036 181 163 2594
Fragments arranged by size:
2594 1036 181 163
BtsI GCAGTG_nn'
Cuts at: 861 1217 861
Size: 356 3618
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DrdI GACnn_nn'nnGTC
Cuts at: 1680 2538 1680
Size: 858 3116
EaeI y'GGCC_r
Cuts at: 464 816 1559 1733 2124 2151 3496 464
Size: 352 743 174 391 27 1345 942
Fragments arranged by size:
1345 942 743 391 352 174 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 2182 2492 2638 3784 360
Size: 1822 310 146 1146 550
Fragments arranged by size:
1822 1146 550 310 146
EcoICRI GAG'CTC
Cuts at: 986 986
Size: 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 592 895 951 1799 2231 2978 3246 244
Size: 348 303 56 848 432 747 268 972
Fragments arranged by size:
972 848 747 432 348 303 268 56
EcoO109I rG'GnC_Cy
Cuts at: 594 595 618 830 881 1113 594
Size: 1 23 212 51 232 3455
Fragments arranged by size:
3455 232 212 51 23 1
EcoRI G'AATT_C
Cuts at: 978 978
Size: 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 495 755 1408 1656 2678 3657 495
Size: 260 653 248 1022 979 812
Fragments arranged by size:
1022 979 812 653 260 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 950 982 1816 1846 1848 1878 349
Size: 32 569 32 834 30 2 30 2445
Fragments arranged by size:
2445 834 569 32 32 30 30 2
HincII GTy'rAC
Cuts at: 129 1013 129
Size: 884 3090
KasI G'GCGC_C
Cuts at: 1404 1652 1404
Size: 248 3726
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2829 2645
Size: 184 3790
MscI TGG'CCA
Cuts at: 1735 1735
Size: 3974
MslI CAynn'nnrTG
Cuts at: 247 250 1963 2090 247
Size: 3 1713 127 2131
Fragments arranged by size:
2131 1713 127 3
MspA1I CmG'CkG
Cuts at: 486 633 770 1085 1399 1759 2772 3017 486
Size: 147 137 315 314 360 1013 245 1443
Fragments arranged by size:
1443 1013 360 315 314 245 147 137
NaeI GCC'GGC
Cuts at: 331 2155 331
Size: 1824 2150
NarI GG'CG_CC
Cuts at: 1405 1653 1405
Size: 248 3726
NgoMIV G'CCGG_C
Cuts at: 329 2153 329
Size: 1824 2150
NspI r_CATG'y
Cuts at: 1027 1527 2434 1027
Size: 500 907 2567
Fragments arranged by size:
2567 907 500
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PflMI CCAn_nnn'nTGG
Cuts at: 207 207
Size: 3974
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PmlI CAC'GTG
Cuts at: 176 284 758 176
Size: 108 474 3392
Fragments arranged by size:
3392 474 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3942
PpuMI rG'GwC_Cy
Cuts at: 618 830 881 618
Size: 212 51 3711
Fragments arranged by size:
3711 212 51
PspOMI G'GGCC_C
Cuts at: 594 594
Size: 3974
PstI C_TGCA'G
Cuts at: 530 1021 1706 530
Size: 491 685 2798
Fragments arranged by size:
2798 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3974
PvuII CAG'CTG
Cuts at: 633 1399 1759 633
Size: 766 360 2848
Fragments arranged by size:
2848 766 360
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 988 988
Size: 3974
SalI G'TCGA_C
Cuts at: 1011 1011
Size: 3974
SanDI GG'GwC_CC
Cuts at: 618 881 618
Size: 263 3711
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SfcI C'TryA_G
Cuts at: 33 526 801 1017 1702 2695 2886 33
Size: 493 275 216 685 993 191 1121
Fragments arranged by size:
1121 993 685 493 275 216 191
SfoI GGC'GCC
Cuts at: 1406 1654 1406
Size: 248 3726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 827 996 827
Size: 169 3805
SmlI C'TyrA_G
Cuts at: 1129 2044 2536 2798 1129
Size: 915 492 262 2305
Fragments arranged by size:
2305 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 1027 1527 1027
Size: 500 3474
StyI C'CwwG_G
Cuts at: 1108 1462 1108
Size: 354 3620
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 3489 3489
Size: 3974
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 3942
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 853 1469 459
Size: 394 616 2964
Fragments arranged by size:
2964 616 394
XmaI C'CCGG_G
Cuts at: 825 994 825
Size: 169 3805
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI