Restriction map for
pF1KE0921
(Circular) (Six-base) MAPSORT of: pF1KE0921.seq Check: 3639 from: 1 to: 3959
pF1KE0921 3959 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 142 975 142
Size: 833 3126
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
AflIII A'CryG_T
Cuts at: 316 1311 2415 3392 316
Size: 995 1104 977 883
Fragments arranged by size:
1104 995 977 883
AgeI A'CCGG_T
Cuts at: 139 2347 139
Size: 2208 1751
AlwNI CAG_nnn'CTG
Cuts at: 1264 2831 1264
Size: 1567 2392
ApaI G_GGCC'C
Cuts at: 340 340
Size: 3959
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 765 963 3312 3568 765
Size: 198 2349 256 1156
Fragments arranged by size:
2349 1156 256 198
AseI AT'TA_AT
Cuts at: 20 617 20
Size: 597 3362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 979 979
Size: 3959
BamHI G'GATC_C
Cuts at: 346 984 346
Size: 638 3321
BanI G'GyrC_C
Cuts at: 142 171 975 1389 1637 1672 142
Size: 29 804 414 248 35 2429
Fragments arranged by size:
2429 804 414 248 35 29
BanII G_rGCy'C
Cuts at: 340 467 973 2003 340
Size: 127 506 1030 2296
Fragments arranged by size:
2296 1030 506 127
BbeI G_GCGC'C
Cuts at: 1393 1641 1393
Size: 248 3711
BbsI GAAGACnn'nnnn_
Cuts at: 404 404
Size: 3959
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BfrBI ATG'CAT
Cuts at: 555 555
Size: 3959
BglII A'GATC_T
Cuts at: 1474 3511 1474
Size: 2037 1922
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 340 499 1584 1677 2733 340
Size: 159 1085 93 1056 1566
Fragments arranged by size:
1566 1085 1056 159 93
BmrI ACTGGGnnnn_n'
Cuts at: 648 1171 1252 1587 3731 648
Size: 523 81 335 2144 876
Fragments arranged by size:
2144 876 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1135 2014 2506 2804 802
Size: 333 879 492 298 1957
Fragments arranged by size:
1957 879 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 3663 3663
Size: 3959
BsaAI yAC'GTr
Cuts at: 1312 1312
Size: 3959
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 1390 1638 2340 3836 1390
Size: 248 702 1496 1513
Fragments arranged by size:
1513 1496 702 248
BsaWI w'CCGG_w
Cuts at: 139 1148 1669 2347 2621 2768 3179 139
Size: 1009 521 678 274 147 411 919
Fragments arranged by size:
1009 919 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 815 815
Size: 3959
BseYI C'CCAG_C
Cuts at: 1384 2142 2719 3215 1384
Size: 758 577 496 2128
Fragments arranged by size:
2128 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 973 1751 1941 2733 467
Size: 506 778 190 792 1693
Fragments arranged by size:
1693 792 778 506 190
Bsp1286I G_dGCh'C
Cuts at: 340 467 499 973 1584 1677 1751 1941 2003
Size: 127 32 474 611 93 74 190 62
Cuts at: 2003 2733 340
Size: 730 1566
Fragments arranged by size:
1566 730 611 474 190 127 93 74
62 32
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 837 3135 837
Size: 2298 1661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1009 1223 1525 1906 1009
Size: 214 302 381 3062
Fragments arranged by size:
3062 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2251 2305 3789 3954 50
Size: 2201 54 1484 165 55
Fragments arranged by size:
2201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 893 1871 893
Size: 978 2981
BsrFI r'CCGG_y
Cuts at: 139 1957 2138 2347 139
Size: 1818 181 209 1751
Fragments arranged by size:
1818 1751 209 181
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstEII G'GTnAC_C
Cuts at: 709 709
Size: 3959
BstYI r'GATC_y
Cuts at: 1 346 984 1451 1474 1809 2055 3067 3153
Size: 345 638 467 23 335 246 1012 86
Cuts at: 3153 3165 3511 1
Size: 12 346 449
Fragments arranged by size:
1012 638 467 449 346 345 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 877 1665 2523 877
Size: 788 858 2313
Fragments arranged by size:
2313 858 788
EaeI y'GGCC_r
Cuts at: 279 1544 1718 2109 2136 3481 279
Size: 1265 174 391 27 1345 757
Fragments arranged by size:
1345 1265 757 391 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 455 1982 2192 455
Size: 1527 210 2222
Fragments arranged by size:
2222 1527 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2167 2477 2623 3769 2167
Size: 310 146 1146 2357
Fragments arranged by size:
2357 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 971 465
Size: 506 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 336 337 1098 336
Size: 1 761 3197
Fragments arranged by size:
3197 761 1
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 1393 1641 2663 3642 1393
Size: 248 1022 979 1710
Fragments arranged by size:
1710 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 1801 1831 1833 1863 267
Size: 32 1502 30 2 30 2363
Fragments arranged by size:
2363 1502 32 30 30 2
HincII GTy'rAC
Cuts at: 636 998 636
Size: 362 3597
KasI G'GCGC_C
Cuts at: 1389 1637 1389
Size: 248 3711
KpnI G_GTAC'C
Cuts at: 146 979 146
Size: 833 3126
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 349 2630 2814 224
Size: 125 2281 184 1369
Fragments arranged by size:
2281 1369 184 125
MscI TGG'CCA
Cuts at: 281 1720 281
Size: 1439 2520
MslI CAynn'nnrTG
Cuts at: 1948 2075 1948
Size: 127 3832
MspA1I CmG'CkG
Cuts at: 738 1070 1384 1744 2757 3002 738
Size: 332 314 360 1013 245 1695
Fragments arranged by size:
1695 1013 360 332 314 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 1390 1638 1390
Size: 248 3711
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NsiI A_TGCA'T
Cuts at: 557 557
Size: 3959
NspI r_CATG'y
Cuts at: 320 1012 1512 2419 320
Size: 692 500 907 1860
Fragments arranged by size:
1860 907 692 500
PciI A'CATG_T
Cuts at: 316 2415 316
Size: 2099 1860
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PspOMI G'GGCC_C
Cuts at: 336 336
Size: 3959
PstI C_TGCA'G
Cuts at: 1006 1691 1006
Size: 685 3274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 738 1384 1744 738
Size: 646 360 2953
Fragments arranged by size:
2953 646 360
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 467 973 467
Size: 506 3453
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 455 1982 2192 455
Size: 1527 210 2222
Fragments arranged by size:
2222 1527 210
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 389 3476 389
Size: 3087 872
SfcI C'TryA_G
Cuts at: 33 125 580 608 1002 1687 2680 2871 33
Size: 92 455 28 394 685 993 191 1121
Fragments arranged by size:
1121 993 685 455 394 191 92 28
SfoI GGC'GCC
Cuts at: 1391 1639 1391
Size: 248 3711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 981 981
Size: 3959
SmlI C'TyrA_G
Cuts at: 781 1114 2029 2521 2783 781
Size: 333 915 492 262 1957
Fragments arranged by size:
1957 915 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
StuI AGG'CCT
Cuts at: 480 480
Size: 3959
StyI C'CwwG_G
Cuts at: 1093 1447 1093
Size: 354 3605
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 630 1542 1675 2134 2343 630
Size: 912 133 459 209 2246
Fragments arranged by size:
2246 912 459 209 133
TatI w'GTAC_w
Cuts at: 387 3474 387
Size: 3087 872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 306 338 3476 3508 306
Size: 32 3138 32 757
Fragments arranged by size:
3138 757 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 207 357 1454 207
Size: 150 1097 2712
Fragments arranged by size:
2712 1097 150
XmaI C'CCGG_G
Cuts at: 979 979
Size: 3959
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI