Restriction map for
pF1KE0922
(Circular) (Six-base) MAPSORT of: pF1KE0922.seq Check: 2165 from: 1 to: 5132
pF1KE0922 5132 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 393 1107 393
Size: 714 4418
AccI GT'mk_AC
Cuts at: 2170 2170
Size: 5132
Acc65I G'GTAC_C
Cuts at: 2148 2148
Size: 5132
AclI AA'CG_TT
Cuts at: 4972 4972
Size: 5132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1081 1186 1378 1581 2113 2957 3389 4136
Size: 297 105 192 203 532 844 432 747
Cuts at: 4136 4404 784
Size: 268 1512
Fragments arranged by size:
1512 844 747 532 432 297 268 203
192 105
AfeI AGC'GCT
Cuts at: 1220 1220
Size: 5132
AflIII A'CryG_T
Cuts at: 1741 2484 3588 4565 1741
Size: 743 1104 977 2308
Fragments arranged by size:
2308 1104 977 743
AgeI A'CCGG_T
Cuts at: 3520 3520
Size: 5132
AhdI GACnn_n'nnGTC
Cuts at: 1359 1359
Size: 5132
AlwNI CAG_nnn'CTG
Cuts at: 2437 4004 2437
Size: 1567 3565
ApaLI G'TGCA_C
Cuts at: 3902 3902
Size: 5132
ApoI r'AATT_y
Cuts at: 2026 2136 4485 4741 2026
Size: 110 2349 256 2417
Fragments arranged by size:
2417 2349 256 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 5132
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
AvaI C'yCGr_G
Cuts at: 423 914 1154 1243 2152 423
Size: 491 240 89 909 3403
Fragments arranged by size:
3403 909 491 240 89
BamHI G'GATC_C
Cuts at: 1604 2157 1604
Size: 553 4579
BanI G'GyrC_C
Cuts at: 127 2148 2562 2810 2845 127
Size: 2021 414 248 35 2414
Fragments arranged by size:
2414 2021 414 248 35
BanII G_rGCy'C
Cuts at: 145 1403 1579 2146 3176 145
Size: 1258 176 567 1030 2101
Fragments arranged by size:
2101 1258 1030 567 176
BbeI G_GCGC'C
Cuts at: 2566 2814 2566
Size: 248 4884
BbsI GAAGACnn'nnnn_
Cuts at: 772 1057 1262 1785 1972 772
Size: 285 205 523 187 3932
Fragments arranged by size:
3932 523 285 205 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 721 755 721
Size: 34 5098
BciVI GTATCCnnnnn_n'
Cuts at: 862 1998 3030 3791 5132 862
Size: 1136 1032 761 1341 862
Fragments arranged by size:
1341 1136 1032 862 761
BclI T'GATC_A
Cuts at: 1019 2652 1019
Size: 1633 3499
BfrBI ATG'CAT
Cuts at: 1873 1873
Size: 5132
BglI GCCn_nnn'nGGC
Cuts at: 1681 2104 1681
Size: 423 4709
BglII A'GATC_T
Cuts at: 458 980 1367 1691 2647 4684 458
Size: 522 387 324 956 2037 906
Fragments arranged by size:
2037 956 906 522 387 324
BlpI GC'TnA_GC
Cuts at: 2244 2244
Size: 5132
Bme1580I G_kGCm'C
Cuts at: 2757 2850 3906 2757
Size: 93 1056 3983
Fragments arranged by size:
3983 1056 93
BmgBI CAC'GTC
Cuts at: 1827 1827
Size: 5132
BmrI ACTGGGnnnn_n'
Cuts at: 693 2344 2425 2760 4904 693
Size: 1651 81 335 2144 921
Fragments arranged by size:
2144 1651 921 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 5132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 577 1394 1582 553
Size: 24 817 188 4103
Fragments arranged by size:
4103 817 188 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2308 3187 3679 3977 2308
Size: 879 492 298 3463
Fragments arranged by size:
3463 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 948 1370 4836 948
Size: 422 3466 1244
Fragments arranged by size:
3466 1244 422
BsaAI yAC'GTr
Cuts at: 2485 2485
Size: 5132
BsaBI GATnn'nnATC
Cuts at: 6 758 934 2646 4683 6
Size: 752 176 1712 2037 455
Fragments arranged by size:
2037 1712 752 455 176
BsaHI Gr'CG_yC
Cuts at: 2563 2811 3513 5009 2563
Size: 248 702 1496 2686
Fragments arranged by size:
2686 1496 702 248
BsaWI w'CCGG_w
Cuts at: 854 986 1784 2018 2080 2321 2842 3520 3794
Size: 132 798 234 62 241 521 678 274
Cuts at: 3794 3941 4352 854
Size: 147 411 1634
Fragments arranged by size:
1634 798 678 521 411 274 241 234
147 132 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 5102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 1651 1658 622
Size: 1029 7 4096
Fragments arranged by size:
4096 1029 7
BseYI C'CCAG_C
Cuts at: 1182 2557 3315 3892 4388 1182
Size: 1375 758 577 496 1926
Fragments arranged by size:
1926 1375 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1166 1166
Size: 5132
BsiEI CG_ry'CG
Cuts at: 104 2674 2720 3928 104
Size: 2570 46 1208 1308
Fragments arranged by size:
2570 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 862 1579 1835 2146 2924 3114 3906 862
Size: 717 256 311 778 190 792 2088
Fragments arranged by size:
2088 792 778 717 311 256 190
Bsp1286I G_dGCh'C
Cuts at: 145 862 1403 1579 1835 2146 2757 2850 2924
Size: 717 541 176 256 311 611 93 74
Cuts at: 2924 3114 3176 3906 145
Size: 190 62 730 1371
Fragments arranged by size:
1371 730 717 611 541 311 256 190
176 93 74 62
BspEI T'CCGG_A
Cuts at: 854 986 1784 2018 2080 4352 854
Size: 132 798 234 62 2272 1634
Fragments arranged by size:
2272 1634 798 234 132 62
BspHI T'CATG_A
Cuts at: 1403 1748 4308 1403
Size: 345 2560 2227
Fragments arranged by size:
2560 2227 345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 1107 2015 2182 2396 2698 3079 393
Size: 714 908 167 214 302 381 2446
Fragments arranged by size:
2446 908 714 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 525 3424 3478 4962 5127 50
Size: 475 2899 54 1484 165 55
Fragments arranged by size:
2899 1484 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 317 1296 3044 317
Size: 979 1748 2405
Fragments arranged by size:
2405 1748 979
BsrFI r'CCGG_y
Cuts at: 1413 1459 3130 3311 3520 1413
Size: 46 1671 181 209 3025
Fragments arranged by size:
3025 1671 209 181 46
BsrGI T'GTAC_A
Cuts at: 194 194
Size: 5132
BssSI C'ACGA_G
Cuts at: 3403 3761 3403
Size: 358 4774
BstAPI GCAn_nnn'nTGC
Cuts at: 127 2509 127
Size: 2382 2750
BstBI TT'CG_AA
Cuts at: 3493 3493
Size: 5132
BstXI CCAn_nnnn'nTGG
Cuts at: 1983 1983
Size: 5132
BstYI r'GATC_y
Cuts at: 1 458 980 1307 1367 1604 1691 2157 2624
Size: 457 522 327 60 237 87 466 467
Cuts at: 2624 2647 2982 3228 4240 4326 4338 4684 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 522 467 466 457 449 346 335
327 246 237 87 86 60 23 12
BtgI C'CryG_G
Cuts at: 107 1448 1622 107
Size: 1341 174 3617
Fragments arranged by size:
3617 1341 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3082 3263 3426 3082
Size: 181 163 4788
Fragments arranged by size:
4788 181 163
BtsI GCAGTG_nn'
Cuts at: 197 225 1154 2001 2375 197
Size: 28 929 847 374 2954
Fragments arranged by size:
2954 929 847 374 28
ClaI AT'CG_AT
Cuts at: 1268 1268
Size: 5132
DraI TTT'AAA
Cuts at: 329 2131 329
Size: 1802 3330
DraIII CAC_nnn'GTG
Cuts at: 127 1717 127
Size: 1590 3542
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2838 3696 1323
Size: 1515 858 2759
Fragments arranged by size:
2759 1515 858
EaeI y'GGCC_r
Cuts at: 110 561 836 1092 1335 1493 1590 1619 1880
Size: 451 275 256 243 158 97 29 261
Cuts at: 1880 2717 2891 3282 3309 4654 110
Size: 837 174 391 27 1345 588
Fragments arranged by size:
1345 837 588 451 391 275 261 256
243 174 158 97 29 27
EagI C'GGCC_G
Cuts at: 2717 2717
Size: 5132
EarI CTCTTCn'nnn_
Cuts at: 892 1918 3155 3365 892
Size: 1026 1237 210 2659
Fragments arranged by size:
2659 1237 1026 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3340 3650 3796 4942 3340
Size: 310 146 1146 3530
Fragments arranged by size:
3530 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1577 2144 1577
Size: 567 4565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 577 784 1081 1186 1378 1394 1581 1582
Size: 24 207 297 105 192 16 187 1
Cuts at: 1582 2113 2957 3389 4136 4404 553
Size: 531 844 432 747 268 1281
Fragments arranged by size:
1281 844 747 531 432 297 268 207
192 187 105 24 16 1
EcoNI CCTnn'n_nnAGG
Cuts at: 592 661 1657 592
Size: 69 996 4067
Fragments arranged by size:
4067 996 69
EcoO109I rG'GnC_Cy
Cuts at: 312 528 630 654 903 2271 312
Size: 216 102 24 249 1368 3173
Fragments arranged by size:
3173 1368 249 216 102 24
EcoRI G'AATT_C
Cuts at: 2026 2136 2026
Size: 110 5022
EcoRV GAT'ATC
Cuts at: 1272 1272
Size: 5132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1347 1929 3151 1347
Size: 582 1222 3328
Fragments arranged by size:
3328 1222 582
FspI TGC'GCA
Cuts at: 2913 2913
Size: 5132
HaeII r_GCGC'y
Cuts at: 1222 2566 2814 3836 4815 1222
Size: 1344 248 1022 979 1539
Fragments arranged by size:
1539 1344 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 1345 1377 2974 3004 3006 3036 406
Size: 32 907 32 1597 30 2 30 2502
Fragments arranged by size:
2502 1597 907 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2171 2171
Size: 5132
KasI G'GCGC_C
Cuts at: 2562 2810 2562
Size: 248 4884
KpnI G_GTAC'C
Cuts at: 2152 2152
Size: 5132
MluI A'CGCG_T
Cuts at: 4565 4565
Size: 5132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 938 3803 3987 938
Size: 2865 184 2083
Fragments arranged by size:
2865 2083 184
MscI TGG'CCA
Cuts at: 112 563 1094 1495 1592 1621 1882 2893 112
Size: 451 531 401 97 29 261 1011 2351
Fragments arranged by size:
2351 1011 531 451 401 261 97 29
MslI CAynn'nnrTG
Cuts at: 202 1424 1810 3121 3248 202
Size: 1222 386 1311 127 2086
Fragments arranged by size:
2086 1311 1222 386 127
MspA1I CmG'CkG
Cuts at: 495 1299 2243 2557 2917 3930 4175 495
Size: 804 944 314 360 1013 245 1452
Fragments arranged by size:
1452 1013 944 804 360 314 245
NaeI GCC'GGC
Cuts at: 1461 3313 1461
Size: 1852 3280
NarI GG'CG_CC
Cuts at: 2563 2811 2563
Size: 248 4884
NcoI C'CATG_G
Cuts at: 107 1448 107
Size: 1341 3791
NgoMIV G'CCGG_C
Cuts at: 1459 3311 1459
Size: 1852 3280
NsiI A_TGCA'T
Cuts at: 1875 1875
Size: 5132
NspI r_CATG'y
Cuts at: 966 2185 2685 3592 966
Size: 1219 500 907 2506
Fragments arranged by size:
2506 1219 907 500
PciI A'CATG_T
Cuts at: 3588 3588
Size: 5132
PflMI CCAn_nnn'nTGG
Cuts at: 498 498
Size: 5132
PfoI T'CCnGG_A
Cuts at: 469 650 1103 1796 469
Size: 181 453 693 3805
Fragments arranged by size:
3805 693 453 181
PmeI GTTT'AAAC
Cuts at: 2131 2131
Size: 5132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 4271
Size: 32 5100
PpuMI rG'GwC_Cy
Cuts at: 312 528 630 654 312
Size: 216 102 24 4790
Fragments arranged by size:
4790 216 102 24
PstI C_TGCA'G
Cuts at: 2179 2864 2179
Size: 685 4447
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5132
PvuII CAG'CTG
Cuts at: 495 2557 2917 495
Size: 2062 360 2710
Fragments arranged by size:
2710 2062 360
RsrII CG'GwC_CG
Cuts at: 3327 3327
Size: 5132
SacI G_AGCT'C
Cuts at: 1579 2146 1579
Size: 567 4565
SalI G'TCGA_C
Cuts at: 2169 2169
Size: 5132
SapI GCTCTTCn'nnn_
Cuts at: 892 3155 3365 892
Size: 2263 210 2659
Fragments arranged by size:
2659 2263 210
SbfI CC_TGCA'GG
Cuts at: 2179 2179
Size: 5132
ScaI AGT'ACT
Cuts at: 4649 4649
Size: 5132
SexAI A'CCwGG_T
Cuts at: 632 632
Size: 5132
SfcI C'TryA_G
Cuts at: 33 1011 2175 2860 3853 4044 33
Size: 978 1164 685 993 191 1121
Fragments arranged by size:
1164 1121 993 978 685 191
SfoI GGC'GCC
Cuts at: 2564 2812 2564
Size: 248 4884
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
SmaI CCC'GGG
Cuts at: 2154 2154
Size: 5132
SmlI C'TyrA_G
Cuts at: 2287 3202 3694 3956 2287
Size: 915 492 262 3463
Fragments arranged by size:
3463 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5132
SphI G_CATG'C
Cuts at: 2185 2685 2185
Size: 500 4632
SspI AAT'ATT
Cuts at: 871 871
Size: 5132
StuI AGG'CCT
Cuts at: 1534 2098 1534
Size: 564 4568
StyI C'CwwG_G
Cuts at: 107 1448 1733 2266 2620 107
Size: 1341 285 533 354 2619
Fragments arranged by size:
2619 1341 533 354 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2715 2848 3307 3516 2715
Size: 133 459 209 4331
Fragments arranged by size:
4331 459 209 133
TatI w'GTAC_w
Cuts at: 194 1043 4647 194
Size: 849 3604 679
Fragments arranged by size:
3604 849 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 5100
Tth111I GACn'n_nGTC
Cuts at: 2929 2929
Size: 5132
XbaI T'CTAG_A
Cuts at: 2163 2163
Size: 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1824 2627 1824
Size: 803 4329
XmaI C'CCGG_G
Cuts at: 2152 2152
Size: 5132
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BmtI Bpu10I BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I CspCI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI