Restriction map for
pF1KE0931
(Circular) (Six-base) MAPSORT of: pF1KE0931.seq Check: 39 from: 1 to: 4283
pF1KE0931 4283 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 499 902 4123 499
Size: 403 3221 659
Fragments arranged by size:
3221 659 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 301 584 808 1151 2108 2540 3287 3555
Size: 52 283 224 343 957 432 747 268
Cuts at: 3555 249
Size: 977
Fragments arranged by size:
977 957 747 432 343 283 268 224
52
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 250 297 917 930 1588 3155 250
Size: 47 620 13 658 1567 1378
Fragments arranged by size:
1567 1378 658 620 47 13
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 233 1303 233
Size: 1070 3213
BamHI G'GATC_C
Cuts at: 424 555 669 836 1308 424
Size: 131 114 167 472 3399
Fragments arranged by size:
3399 472 167 131 114
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 824 1297 2327 824
Size: 473 1030 2780
Fragments arranged by size:
2780 1030 473
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 730 764 730
Size: 34 4249
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 1908 2001 3057 1908
Size: 93 1056 3134
Fragments arranged by size:
3134 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 365
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 711 711
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 816 1226 1459 2338 2830 3128 469
Size: 347 410 233 879 492 298 1624
Fragments arranged by size:
1624 879 492 410 347 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 1636 1636
Size: 4283
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1142 1172 1142
Size: 30 4253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 170 182 1234 170
Size: 12 1052 3219
Fragments arranged by size:
3219 1052 12
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 552 1297 2075 2265 3057 552
Size: 745 778 190 792 1778
Fragments arranged by size:
1778 792 778 745 190
Bsp1286I G_dGCh'C
Cuts at: 552 824 1297 1908 2001 2075 2265 2327 3057
Size: 272 473 611 93 74 190 62 730
Cuts at: 3057 552
Size: 1778
Fragments arranged by size:
1778 730 611 473 272 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 1010 3459 1010
Size: 2449 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 2281 2462 2671 2281
Size: 181 209 3893
Fragments arranged by size:
3893 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 660 2644 660
Size: 1984 2299
BstYI r'GATC_y
Cuts at: 1 424 555 669 836 1091 1308 1775 1798
Size: 423 131 114 167 255 217 467 23
Cuts at: 1798 2133 2379 3391 3477 3489 3835 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 255 246
217 167 131 114 86 23 12
BtgI C'CryG_G
Cuts at: 126 126
Size: 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 1526 1526
Size: 4283
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 462 1078 462
Size: 616 3667
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 1098 1868 2042 2433 2460 3805 1098
Size: 770 174 391 27 1345 1576
Fragments arranged by size:
1576 1345 770 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 277 1211 2306 2516 277
Size: 934 1095 210 2044
Fragments arranged by size:
2044 1095 934 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 301 365 584 808 1151 2108 2540 3287
Size: 52 64 219 224 343 957 432 747
Cuts at: 3287 3555 249
Size: 268 977
Fragments arranged by size:
977 957 747 432 343 268 224 219
64 52
EcoNI CCTnn'n_nnAGG
Cuts at: 1099 1099
Size: 4283
EcoO109I rG'GnC_Cy
Cuts at: 179 294 1422 179
Size: 115 1128 3040
Fragments arranged by size:
3040 1128 115
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
EcoRV GAT'ATC
Cuts at: 690 690
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 144 918 1717 1965 2987 3966 144
Size: 774 799 248 1022 979 461
Fragments arranged by size:
1022 979 799 774 461 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 363 383 395 2125 2155 2157 2187 351
Size: 12 20 12 1730 30 2 30 2447
Fragments arranged by size:
2447 1730 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 747 2954 3138 482
Size: 265 2207 184 1627
Fragments arranged by size:
2207 1627 265 184
MscI TGG'CCA
Cuts at: 1100 2044 1100
Size: 944 3339
MslI CAynn'nnrTG
Cuts at: 1009 2272 2399 1009
Size: 1263 127 2893
Fragments arranged by size:
2893 1263 127
MspA1I CmG'CkG
Cuts at: 734 1394 1708 2068 3081 3326 734
Size: 660 314 360 1013 245 1691
Fragments arranged by size:
1691 1013 660 360 314 245
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 730 1336 1836 2743 730
Size: 606 500 907 2270
Fragments arranged by size:
2270 907 606 500
PasI CC'CwG_GG
Cuts at: 519 519
Size: 4283
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 4283
PshAI GACnn'nnGTC
Cuts at: 1057 1057
Size: 4283
PstI C_TGCA'G
Cuts at: 939 1330 2015 939
Size: 391 685 3207
Fragments arranged by size:
3207 685 391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1708 2068 1708
Size: 360 3923
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SexAI A'CCwGG_T
Cuts at: 470 470
Size: 4283
SfcI C'TryA_G
Cuts at: 33 449 620 935 1326 2011 3004 3195 33
Size: 416 171 315 391 685 993 191 1121
Fragments arranged by size:
1121 993 685 416 391 315 191 171
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 235 1305 235
Size: 1070 3213
SmlI C'TyrA_G
Cuts at: 484 795 1241 1438 2353 2845 3107 484
Size: 311 446 197 915 492 262 1660
Fragments arranged by size:
1660 915 492 446 311 262 197
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
StuI AGG'CCT
Cuts at: 274 274
Size: 4283
StyI C'CwwG_G
Cuts at: 1095 1417 1771 1095
Size: 322 354 3607
Fragments arranged by size:
3607 354 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 666 1866 1999 2458 2667 666
Size: 1200 133 459 209 2282
Fragments arranged by size:
2282 1200 459 209 133
TatI w'GTAC_w
Cuts at: 999 3798 999
Size: 2799 1484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 821 853 3800 3832 446
Size: 32 343 32 2947 32 897
Fragments arranged by size:
2947 897 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1033 2080 1033
Size: 1047 3236
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 888 1778 888
Size: 890 3393
XmaI C'CCGG_G
Cuts at: 233 1303 233
Size: 1070 3213
XmnI GAAnn'nnTTC
Cuts at: 410 410
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI