Restriction map for
pF1KE0937
(Circular) (Six-base) MAPSORT of: pF1KE0937.seq Check: 9891 from: 1 to: 4397
pF1KE0937 4397 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 353 1435 353
Size: 1082 3315
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 2222 2654 3401 3669 758
Size: 1464 432 747 268 1486
Fragments arranged by size:
1486 1464 747 432 268
AflIII A'CryG_T
Cuts at: 1749 2853 3830 1749
Size: 1104 977 2316
Fragments arranged by size:
2316 1104 977
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AlwNI CAG_nnn'CTG
Cuts at: 138 150 606 664 1702 3269 138
Size: 12 456 58 1038 1567 1266
Fragments arranged by size:
1567 1266 1038 456 58 12
ApaI G_GGCC'C
Cuts at: 866 1382 866
Size: 516 3881
ApaLI G'TGCA_C
Cuts at: 1321 3167 1321
Size: 1846 2551
ApoI r'AATT_y
Cuts at: 205 1401 3750 4006 205
Size: 1196 2349 256 596
Fragments arranged by size:
2349 1196 596 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 573 687 778 1055 1080 1417 573
Size: 114 91 277 25 337 3553
Fragments arranged by size:
3553 337 277 114 91 25
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 1413 1827 2075 2110 1413
Size: 414 248 35 3700
Fragments arranged by size:
3700 414 248 35
BanII G_rGCy'C
Cuts at: 866 1382 1411 2441 866
Size: 516 29 1030 2822
Fragments arranged by size:
2822 1030 516 29
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 1917 1917
Size: 4397
BglI GCCn_nnn'nGGC
Cuts at: 960 960
Size: 4397
BglII A'GATC_T
Cuts at: 1025 1912 3949 1025
Size: 887 2037 1473
Fragments arranged by size:
2037 1473 887
BlpI GC'TnA_GC
Cuts at: 286 1509 286
Size: 1223 3174
Bme1580I G_kGCm'C
Cuts at: 866 1325 1382 2022 2115 3171 866
Size: 459 57 640 93 1056 2092
Fragments arranged by size:
2092 1056 640 459 93 57
BmrI ACTGGGnnnn_n'
Cuts at: 647 1609 1690 2025 4169 647
Size: 962 81 335 2144 875
Fragments arranged by size:
2144 962 875 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 4397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1126 1263 751
Size: 375 137 3885
Fragments arranged by size:
3885 375 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1573 2452 2944 3242 1573
Size: 879 492 298 2728
Fragments arranged by size:
2728 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 1750 1750
Size: 4397
BsaBI GATnn'nnATC
Cuts at: 6 1911 3948 6
Size: 1905 2037 455
Fragments arranged by size:
2037 1905 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1586 2107 2785 3059 3206 3617 1586
Size: 521 678 274 147 411 2366
Fragments arranged by size:
2366 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 690 720 883 913 163
Size: 30 497 30 163 30 3647
Fragments arranged by size:
3647 497 163 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 241
Size: 4397
BseYI C'CCAG_C
Cuts at: 410 1031 1213 1822 2580 3157 3653 410
Size: 621 182 609 758 577 496 1154
Fragments arranged by size:
1154 758 621 609 577 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 677 536
Size: 141 4256
BsiEI CG_ry'CG
Cuts at: 104 1939 1985 3193 104
Size: 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 391 650 703 1249 1325 1370 1411 2189 2379
Size: 259 53 546 76 45 41 778 190
Cuts at: 2379 3171 391
Size: 792 1617
Fragments arranged by size:
1617 792 778 546 259 190 76 53
45 41
BsmI GAATG_Cn'
Cuts at: 1232 1232
Size: 4397
Bsp1286I G_dGCh'C
Cuts at: 391 650 703 866 1249 1325 1370 1382 1411
Size: 259 53 163 383 76 45 12 29
Cuts at: 1411 2022 2115 2189 2379 2441 3171 391
Size: 611 93 74 190 62 730 1617
Fragments arranged by size:
1617 730 611 383 259 190 163 93
76 74 62 53 45 29 12
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 3573 3573
Size: 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 750 1447 1661 1963 2344 750
Size: 697 214 302 381 2803
Fragments arranged by size:
2803 697 381 302 214
BsrBI CCG'CTC
Cuts at: 50 338 2689 2743 4227 4392 50
Size: 288 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 288 165 55 54
BsrDI GCAATG_nn'
Cuts at: 171 1259 2309 171
Size: 1088 1050 2259
Fragments arranged by size:
2259 1088 1050
BsrFI r'CCGG_y
Cuts at: 745 1155 2395 2576 2785 745
Size: 410 1240 181 209 2357
Fragments arranged by size:
2357 1240 410 209 181
BssSI C'ACGA_G
Cuts at: 2668 3026 2668
Size: 358 4039
BstAPI GCAn_nnn'nTGC
Cuts at: 138 664 1774 138
Size: 526 1110 2761
Fragments arranged by size:
2761 1110 526
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstYI r'GATC_y
Cuts at: 1 1025 1422 1889 1912 2247 2493 3505 3591
Size: 1024 397 467 23 335 246 1012 86
Cuts at: 3591 3603 3949 1
Size: 12 346 449
Fragments arranged by size:
1024 1012 467 449 397 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 374 374
Size: 4397
BtgI C'CryG_G
Cuts at: 107 968 1361 107
Size: 861 393 3143
Fragments arranged by size:
3143 861 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2347 2528 2691 2347
Size: 181 163 4053
Fragments arranged by size:
4053 181 163
BtsI GCAGTG_nn'
Cuts at: 1640 1640
Size: 4397
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 168 339 1002 1277 1982 2156 2547 2574 3919
Size: 171 663 275 705 174 391 27 1345
Cuts at: 3919 168
Size: 646
Fragments arranged by size:
1345 705 663 646 391 275 174 171
27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 734 797 866 2420 2630 734
Size: 63 69 1554 210 2501
Fragments arranged by size:
2501 1554 210 69 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2605 2915 3061 4207 2605
Size: 310 146 1146 2795
Fragments arranged by size:
2795 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1409 1409
Size: 4397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 758 1126 1263 2222 2654 3401 3669 751
Size: 7 368 137 959 432 747 268 1479
Fragments arranged by size:
1479 959 747 432 368 268 137 7
EcoO109I rG'GnC_Cy
Cuts at: 783 862 1059 1102 1378 1379 1536 783
Size: 79 197 43 276 1 157 3644
Fragments arranged by size:
3644 276 197 157 79 43 1
EcoRI G'AATT_C
Cuts at: 205 1401 205
Size: 1196 3201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1305 2416 1305
Size: 1111 3286
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 682 1162 1316 1831 2079 3101 4080 682
Size: 480 154 515 248 1022 979 999
Fragments arranged by size:
1022 999 979 515 480 248 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 690 722 1336 1368 2239 2269 2271
Size: 32 186 32 614 32 871 30 2
Cuts at: 2271 2301 472
Size: 30 2568
Fragments arranged by size:
2568 871 614 186 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1436 1436
Size: 4397
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 857 3068 3252 857
Size: 2211 184 2002
Fragments arranged by size:
2211 2002 184
MscI TGG'CCA
Cuts at: 170 2158 170
Size: 1988 2409
MslI CAynn'nnrTG
Cuts at: 2386 2513 2386
Size: 127 4270
MspA1I CmG'CkG
Cuts at: 147 970 1508 1822 2182 3195 3440 147
Size: 823 538 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 823 538 360 314 245
NaeI GCC'GGC
Cuts at: 747 1157 2578 747
Size: 410 1421 2566
Fragments arranged by size:
2566 1421 410
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 107
Size: 4397
NgoMIV G'CCGG_C
Cuts at: 745 1155 2576 745
Size: 410 1421 2566
Fragments arranged by size:
2566 1421 410
NspI r_CATG'y
Cuts at: 469 570 1450 1950 2857 469
Size: 101 880 500 907 2009
Fragments arranged by size:
2009 907 880 500 101
PasI CC'CwG_GG
Cuts at: 475 1375 475
Size: 900 3497
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PflMI CCAn_nnn'nTGG
Cuts at: 1306 1306
Size: 4397
PfoI T'CCnGG_A
Cuts at: 181 181
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PpuMI rG'GwC_Cy
Cuts at: 1059 1102 1059
Size: 43 4354
PspOMI G'GGCC_C
Cuts at: 862 1378 862
Size: 516 3881
PstI C_TGCA'G
Cuts at: 146 611 669 761 1444 2129 146
Size: 465 58 92 683 685 2414
Fragments arranged by size:
2414 685 683 465 92 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 147 1822 2182 147
Size: 1675 360 2362
Fragments arranged by size:
2362 1675 360
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 1411 1411
Size: 4397
SacII CC_GC'GG
Cuts at: 971 971
Size: 4397
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SanDI GG'GwC_CC
Cuts at: 1102 1102
Size: 4397
SapI GCTCTTCn'nnn_
Cuts at: 797 2420 2630 797
Size: 1623 210 2564
Fragments arranged by size:
2564 1623 210
SbfI CC_TGCA'GG
Cuts at: 669 1444 669
Size: 775 3622
ScaI AGT'ACT
Cuts at: 3914 3914
Size: 4397
SfcI C'TryA_G
Cuts at: 33 142 607 665 757 1440 2125 3118 3309
Size: 109 465 58 92 683 685 993 191
Cuts at: 3309 33
Size: 1121
Fragments arranged by size:
1121 993 685 683 465 191 109 92
58
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 1057 1419 1057
Size: 362 4035
SmlI C'TyrA_G
Cuts at: 1552 2467 2959 3221 1552
Size: 915 492 262 2728
Fragments arranged by size:
2728 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 1450 1950 1450
Size: 500 3897
StuI AGG'CCT
Cuts at: 724 724
Size: 4397
StyI C'CwwG_G
Cuts at: 107 509 716 866 917 1531 1885 107
Size: 402 207 150 51 614 354 2619
Fragments arranged by size:
2619 614 402 354 207 150 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 1227 1980 2113 2572 2781 522
Size: 705 753 133 459 209 2138
Fragments arranged by size:
2138 753 705 459 209 133
TatI w'GTAC_w
Cuts at: 563 3912 563
Size: 3349 1048
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 1239 1271 3914 3946 163
Size: 32 1044 32 2643 32 614
Fragments arranged by size:
2643 1044 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 484 2194 484
Size: 1710 2687
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 873 1892 444
Size: 429 1019 2949
Fragments arranged by size:
2949 1019 429
XmaI C'CCGG_G
Cuts at: 1055 1417 1055
Size: 362 4035
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI