(Circular) (Six-base) MAPSORT of: pF1KE0937.seq Check: 9891 from: 1 to: 4397 pF1KE0937 4397 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AccI GT'mk_AC Cuts at: 353 1435 353 Size: 1082 3315 Acc65I G'GTAC_C Cuts at: 1413 1413 Size: 4397 AclI AA'CG_TT Cuts at: 4237 4237 Size: 4397 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 758 2222 2654 3401 3669 758 Size: 1464 432 747 268 1486 Fragments arranged by size: 1486 1464 747 432 268 AflIII A'CryG_T Cuts at: 1749 2853 3830 1749 Size: 1104 977 2316 Fragments arranged by size: 2316 1104 977 AgeI A'CCGG_T Cuts at: 2785 2785 Size: 4397 AlwNI CAG_nnn'CTG Cuts at: 138 150 606 664 1702 3269 138 Size: 12 456 58 1038 1567 1266 Fragments arranged by size: 1567 1266 1038 456 58 12 ApaI G_GGCC'C Cuts at: 866 1382 866 Size: 516 3881 ApaLI G'TGCA_C Cuts at: 1321 3167 1321 Size: 1846 2551 ApoI r'AATT_y Cuts at: 205 1401 3750 4006 205 Size: 1196 2349 256 596 Fragments arranged by size: 2349 1196 596 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4397 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4397 AvaI C'yCGr_G Cuts at: 573 687 778 1055 1080 1417 573 Size: 114 91 277 25 337 3553 Fragments arranged by size: 3553 337 277 114 91 25 BamHI G'GATC_C Cuts at: 1422 1422 Size: 4397 BanI G'GyrC_C Cuts at: 1413 1827 2075 2110 1413 Size: 414 248 35 3700 Fragments arranged by size: 3700 414 248 35 BanII G_rGCy'C Cuts at: 866 1382 1411 2441 866 Size: 516 29 1030 2822 Fragments arranged by size: 2822 1030 516 29 BbeI G_GCGC'C Cuts at: 1831 2079 1831 Size: 248 4149 BciVI GTATCCnnnnn_n' Cuts at: 2295 3056 4397 2295 Size: 761 1341 2295 Fragments arranged by size: 2295 1341 761 BclI T'GATC_A Cuts at: 1917 1917 Size: 4397 BglI GCCn_nnn'nGGC Cuts at: 960 960 Size: 4397 BglII A'GATC_T Cuts at: 1025 1912 3949 1025 Size: 887 2037 1473 Fragments arranged by size: 2037 1473 887 BlpI GC'TnA_GC Cuts at: 286 1509 286 Size: 1223 3174 Bme1580I G_kGCm'C Cuts at: 866 1325 1382 2022 2115 3171 866 Size: 459 57 640 93 1056 2092 Fragments arranged by size: 2092 1056 640 459 93 57 BmrI ACTGGGnnnn_n' Cuts at: 647 1609 1690 2025 4169 647 Size: 962 81 335 2144 875 Fragments arranged by size: 2144 962 875 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 4397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 751 1126 1263 751 Size: 375 137 3885 Fragments arranged by size: 3885 375 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1573 2452 2944 3242 1573 Size: 879 492 298 2728 Fragments arranged by size: 2728 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4101 4101 Size: 4397 BsaAI yAC'GTr Cuts at: 1750 1750 Size: 4397 BsaBI GATnn'nnATC Cuts at: 6 1911 3948 6 Size: 1905 2037 455 Fragments arranged by size: 2037 1905 455 BsaHI Gr'CG_yC Cuts at: 1828 2076 2778 4274 1828 Size: 248 702 1496 1951 Fragments arranged by size: 1951 1496 702 248 BsaWI w'CCGG_w Cuts at: 1586 2107 2785 3059 3206 3617 1586 Size: 521 678 274 147 411 2366 Fragments arranged by size: 2366 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 163 193 690 720 883 913 163 Size: 30 497 30 163 30 3647 Fragments arranged by size: 3647 497 163 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 241 241 Size: 4397 BseYI C'CCAG_C Cuts at: 410 1031 1213 1822 2580 3157 3653 410 Size: 621 182 609 758 577 496 1154 Fragments arranged by size: 1154 758 621 609 577 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 536 677 536 Size: 141 4256 BsiEI CG_ry'CG Cuts at: 104 1939 1985 3193 104 Size: 1835 46 1208 1308 Fragments arranged by size: 1835 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 391 650 703 1249 1325 1370 1411 2189 2379 Size: 259 53 546 76 45 41 778 190 Cuts at: 2379 3171 391 Size: 792 1617 Fragments arranged by size: 1617 792 778 546 259 190 76 53 45 41 BsmI GAATG_Cn' Cuts at: 1232 1232 Size: 4397 Bsp1286I G_dGCh'C Cuts at: 391 650 703 866 1249 1325 1370 1382 1411 Size: 259 53 163 383 76 45 12 29 Cuts at: 1411 2022 2115 2189 2379 2441 3171 391 Size: 611 93 74 190 62 730 1617 Fragments arranged by size: 1617 730 611 383 259 190 163 93 76 74 62 53 45 29 12 BspEI T'CCGG_A Cuts at: 3617 3617 Size: 4397 BspHI T'CATG_A Cuts at: 3573 3573 Size: 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 750 1447 1661 1963 2344 750 Size: 697 214 302 381 2803 Fragments arranged by size: 2803 697 381 302 214 BsrBI CCG'CTC Cuts at: 50 338 2689 2743 4227 4392 50 Size: 288 2351 54 1484 165 55 Fragments arranged by size: 2351 1484 288 165 55 54 BsrDI GCAATG_nn' Cuts at: 171 1259 2309 171 Size: 1088 1050 2259 Fragments arranged by size: 2259 1088 1050 BsrFI r'CCGG_y Cuts at: 745 1155 2395 2576 2785 745 Size: 410 1240 181 209 2357 Fragments arranged by size: 2357 1240 410 209 181 BssSI C'ACGA_G Cuts at: 2668 3026 2668 Size: 358 4039 BstAPI GCAn_nnn'nTGC Cuts at: 138 664 1774 138 Size: 526 1110 2761 Fragments arranged by size: 2761 1110 526 BstBI TT'CG_AA Cuts at: 2758 2758 Size: 4397 BstYI r'GATC_y Cuts at: 1 1025 1422 1889 1912 2247 2493 3505 3591 Size: 1024 397 467 23 335 246 1012 86 Cuts at: 3591 3603 3949 1 Size: 12 346 449 Fragments arranged by size: 1024 1012 467 449 397 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 374 374 Size: 4397 BtgI C'CryG_G Cuts at: 107 968 1361 107 Size: 861 393 3143 Fragments arranged by size: 3143 861 393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2347 2528 2691 2347 Size: 181 163 4053 Fragments arranged by size: 4053 181 163 BtsI GCAGTG_nn' Cuts at: 1640 1640 Size: 4397 DraI TTT'AAA Cuts at: 1396 1396 Size: 4397 DrdI GACnn_nn'nnGTC Cuts at: 2103 2961 2103 Size: 858 3539 EaeI y'GGCC_r Cuts at: 168 339 1002 1277 1982 2156 2547 2574 3919 Size: 171 663 275 705 174 391 27 1345 Cuts at: 3919 168 Size: 646 Fragments arranged by size: 1345 705 663 646 391 275 174 171 27 EagI C'GGCC_G Cuts at: 1982 1982 Size: 4397 EarI CTCTTCn'nnn_ Cuts at: 734 797 866 2420 2630 734 Size: 63 69 1554 210 2501 Fragments arranged by size: 2501 1554 210 69 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2605 2915 3061 4207 2605 Size: 310 146 1146 2795 Fragments arranged by size: 2795 1146 310 146 EcoICRI GAG'CTC Cuts at: 1409 1409 Size: 4397 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 751 758 1126 1263 2222 2654 3401 3669 751 Size: 7 368 137 959 432 747 268 1479 Fragments arranged by size: 1479 959 747 432 368 268 137 7 EcoO109I rG'GnC_Cy Cuts at: 783 862 1059 1102 1378 1379 1536 783 Size: 79 197 43 276 1 157 3644 Fragments arranged by size: 3644 276 197 157 79 43 1 EcoRI G'AATT_C Cuts at: 205 1401 205 Size: 1196 3201 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1305 2416 1305 Size: 1111 3286 FspI TGC'GCA Cuts at: 2178 2178 Size: 4397 HaeII r_GCGC'y Cuts at: 682 1162 1316 1831 2079 3101 4080 682 Size: 480 154 515 248 1022 979 999 Fragments arranged by size: 1022 999 979 515 480 248 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 472 504 690 722 1336 1368 2239 2269 2271 Size: 32 186 32 614 32 871 30 2 Cuts at: 2271 2301 472 Size: 30 2568 Fragments arranged by size: 2568 871 614 186 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1436 1436 Size: 4397 KasI G'GCGC_C Cuts at: 1827 2075 1827 Size: 248 4149 KpnI G_GTAC'C Cuts at: 1417 1417 Size: 4397 MluI A'CGCG_T Cuts at: 3830 3830 Size: 4397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 857 3068 3252 857 Size: 2211 184 2002 Fragments arranged by size: 2211 2002 184 MscI TGG'CCA Cuts at: 170 2158 170 Size: 1988 2409 MslI CAynn'nnrTG Cuts at: 2386 2513 2386 Size: 127 4270 MspA1I CmG'CkG Cuts at: 147 970 1508 1822 2182 3195 3440 147 Size: 823 538 314 360 1013 245 1104 Fragments arranged by size: 1104 1013 823 538 360 314 245 NaeI GCC'GGC Cuts at: 747 1157 2578 747 Size: 410 1421 2566 Fragments arranged by size: 2566 1421 410 NarI GG'CG_CC Cuts at: 1828 2076 1828 Size: 248 4149 NcoI C'CATG_G Cuts at: 107 107 Size: 4397 NgoMIV G'CCGG_C Cuts at: 745 1155 2576 745 Size: 410 1421 2566 Fragments arranged by size: 2566 1421 410 NspI r_CATG'y Cuts at: 469 570 1450 1950 2857 469 Size: 101 880 500 907 2009 Fragments arranged by size: 2009 907 880 500 101 PasI CC'CwG_GG Cuts at: 475 1375 475 Size: 900 3497 PciI A'CATG_T Cuts at: 2853 2853 Size: 4397 PflMI CCAn_nnn'nTGG Cuts at: 1306 1306 Size: 4397 PfoI T'CCnGG_A Cuts at: 181 181 Size: 4397 PmeI GTTT'AAAC Cuts at: 1396 1396 Size: 4397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 3536 Size: 32 4365 PpuMI rG'GwC_Cy Cuts at: 1059 1102 1059 Size: 43 4354 PspOMI G'GGCC_C Cuts at: 862 1378 862 Size: 516 3881 PstI C_TGCA'G Cuts at: 146 611 669 761 1444 2129 146 Size: 465 58 92 683 685 2414 Fragments arranged by size: 2414 685 683 465 92 58 PvuI CG_AT'CG Cuts at: 104 104 Size: 4397 PvuII CAG'CTG Cuts at: 147 1822 2182 147 Size: 1675 360 2362 Fragments arranged by size: 2362 1675 360 RsrII CG'GwC_CG Cuts at: 2592 2592 Size: 4397 SacI G_AGCT'C Cuts at: 1411 1411 Size: 4397 SacII CC_GC'GG Cuts at: 971 971 Size: 4397 SalI G'TCGA_C Cuts at: 1434 1434 Size: 4397 SanDI GG'GwC_CC Cuts at: 1102 1102 Size: 4397 SapI GCTCTTCn'nnn_ Cuts at: 797 2420 2630 797 Size: 1623 210 2564 Fragments arranged by size: 2564 1623 210 SbfI CC_TGCA'GG Cuts at: 669 1444 669 Size: 775 3622 ScaI AGT'ACT Cuts at: 3914 3914 Size: 4397 SfcI C'TryA_G Cuts at: 33 142 607 665 757 1440 2125 3118 3309 Size: 109 465 58 92 683 685 993 191 Cuts at: 3309 33 Size: 1121 Fragments arranged by size: 1121 993 685 683 465 191 109 92 58 SfoI GGC'GCC Cuts at: 1829 2077 1829 Size: 248 4149 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4397 SmaI CCC'GGG Cuts at: 1057 1419 1057 Size: 362 4035 SmlI C'TyrA_G Cuts at: 1552 2467 2959 3221 1552 Size: 915 492 262 2728 Fragments arranged by size: 2728 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4397 SphI G_CATG'C Cuts at: 1450 1950 1450 Size: 500 3897 StuI AGG'CCT Cuts at: 724 724 Size: 4397 StyI C'CwwG_G Cuts at: 107 509 716 866 917 1531 1885 107 Size: 402 207 150 51 614 354 2619 Fragments arranged by size: 2619 614 402 354 207 150 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 522 1227 1980 2113 2572 2781 522 Size: 705 753 133 459 209 2138 Fragments arranged by size: 2138 753 705 459 209 133 TatI w'GTAC_w Cuts at: 563 3912 563 Size: 3349 1048 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 163 195 1239 1271 3914 3946 163 Size: 32 1044 32 2643 32 614 Fragments arranged by size: 2643 1044 614 32 32 32 Tth111I GACn'n_nGTC Cuts at: 484 2194 484 Size: 1710 2687 XbaI T'CTAG_A Cuts at: 1428 1428 Size: 4397 XcmI CCAnnnn_n'nnnnTGG Cuts at: 444 873 1892 444 Size: 429 1019 2949 Fragments arranged by size: 2949 1019 429 XmaI C'CCGG_G Cuts at: 1055 1417 1055 Size: 362 4035 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI