Restriction map for
pF1KE0940
(Circular) (Six-base) MAPSORT of: pF1KE0940.seq Check: 6847 from: 1 to: 4490
pF1KE0940 4490 bp
With 182 enzymes: SgfI *
August 9, 2016 17:30 ..
AccI GT'mk_AC
Cuts at: 1528 1528
Size: 4490
Acc65I G'GTAC_C
Cuts at: 1506 1506
Size: 4490
AclI AA'CG_TT
Cuts at: 4330 4330
Size: 4490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1243 2315 2747 3494 3762 1012
Size: 231 1072 432 747 268 1740
Fragments arranged by size:
1740 1072 747 432 268 231
AflIII A'CryG_T
Cuts at: 184 784 1287 1842 2946 3923 184
Size: 600 503 555 1104 977 751
Fragments arranged by size:
1104 977 751 600 555 503
AgeI A'CCGG_T
Cuts at: 2878 2878
Size: 4490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 245 908 245
Size: 663 3827
AlwNI CAG_nnn'CTG
Cuts at: 417 1795 3362 417
Size: 1378 1567 1545
Fragments arranged by size:
1567 1545 1378
ApaLI G'TGCA_C
Cuts at: 3260 3260
Size: 4490
ApoI r'AATT_y
Cuts at: 140 980 1149 1268 1494 3843 4099 140
Size: 840 169 119 226 2349 256 531
Fragments arranged by size:
2349 840 531 256 226 169 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 4490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
AvaI C'yCGr_G
Cuts at: 1510 1510
Size: 4490
AvrII C'CTAG_G
Cuts at: 324 324
Size: 4490
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 656 689 656
Size: 33 4457
BamHI G'GATC_C
Cuts at: 164 1515 164
Size: 1351 3139
BanI G'GyrC_C
Cuts at: 1506 1920 2168 2203 1506
Size: 414 248 35 3793
Fragments arranged by size:
3793 414 248 35
BanII G_rGCy'C
Cuts at: 965 1454 1504 2534 965
Size: 489 50 1030 2921
Fragments arranged by size:
2921 1030 489 50
BbeI G_GCGC'C
Cuts at: 1924 2172 1924
Size: 248 4242
BbvCI CC'TCA_GC
Cuts at: 392 392
Size: 4490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 733 767 90
Size: 34 609 34 3813
Fragments arranged by size:
3813 609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2388 3149 4490 2388
Size: 761 1341 2388
Fragments arranged by size:
2388 1341 761
BclI T'GATC_A
Cuts at: 603 975 2010 603
Size: 372 1035 3083
Fragments arranged by size:
3083 1035 372
BglII A'GATC_T
Cuts at: 2005 4042 2005
Size: 2037 2453
BlpI GC'TnA_GC
Cuts at: 1602 1602
Size: 4490
Bme1580I G_kGCm'C
Cuts at: 542 2115 2208 3264 542
Size: 1573 93 1056 1768
Fragments arranged by size:
1768 1573 1056 93
BmgBI CAC'GTC
Cuts at: 543 543
Size: 4490
BmrI ACTGGGnnnn_n'
Cuts at: 1702 1783 2118 4262 1702
Size: 81 335 2144 1930
Fragments arranged by size:
2144 1930 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1199
Size: 4490
Bpu10I CC'TnA_GC
Cuts at: 392 496 392
Size: 104 4386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1666 2545 3037 3335 1666
Size: 879 492 298 2821
Fragments arranged by size:
2821 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 508 1312 4194 508
Size: 804 2882 804
Fragments arranged by size:
2882 804 804
BsaAI yAC'GTr
Cuts at: 924 1843 924
Size: 919 3571
BsaBI GATnn'nnATC
Cuts at: 6 2004 4041 6
Size: 1998 2037 455
Fragments arranged by size:
2037 1998 455
BsaHI Gr'CG_yC
Cuts at: 1921 2169 2871 4367 1921
Size: 248 702 1496 2044
Fragments arranged by size:
2044 1496 702 248
BsaWI w'CCGG_w
Cuts at: 586 1679 2200 2878 3152 3299 3710 586
Size: 1093 521 678 274 147 411 1366
Fragments arranged by size:
1366 1093 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 1189 1192 228
Size: 961 3 3526
Fragments arranged by size:
3526 961 3
BseYI C'CCAG_C
Cuts at: 1915 2673 3250 3746 1915
Size: 758 577 496 2659
Fragments arranged by size:
2659 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 793 418
Size: 375 4115
BsiEI CG_ry'CG
Cuts at: 104 747 2032 2078 3286 104
Size: 643 1285 46 1208 1308
Fragments arranged by size:
1308 1285 1208 643 46
BsiHKAI G_wGCw'C
Cuts at: 117 965 1454 1504 2282 2472 3264 117
Size: 848 489 50 778 190 792 1343
Fragments arranged by size:
1343 848 792 778 489 190 50
BsmI GAATG_Cn'
Cuts at: 1091 1091
Size: 4490
Bsp1286I G_dGCh'C
Cuts at: 117 542 965 1454 1504 2115 2208 2282 2472
Size: 425 423 489 50 611 93 74 190
Cuts at: 2472 2534 3264 117
Size: 62 730 1343
Fragments arranged by size:
1343 730 611 489 425 423 190 93
74 62 50
BspEI T'CCGG_A
Cuts at: 3710 3710
Size: 4490
BspHI T'CATG_A
Cuts at: 3666 3666
Size: 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 560 1380 1540 1754 2056 2437 560
Size: 820 160 214 302 381 2613
Fragments arranged by size:
2613 820 381 302 214 160
BsrBI CCG'CTC
Cuts at: 50 2782 2836 4320 4485 50
Size: 2732 54 1484 165 55
Fragments arranged by size:
2732 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2402 2402
Size: 4490
BsrFI r'CCGG_y
Cuts at: 1474 2488 2669 2878 1474
Size: 1014 181 209 3086
Fragments arranged by size:
3086 1014 209 181
BsrGI T'GTAC_A
Cuts at: 470 470
Size: 4490
BssSI C'ACGA_G
Cuts at: 2761 3119 2761
Size: 358 4132
BstAPI GCAn_nnn'nTGC
Cuts at: 417 1867 417
Size: 1450 3040
BstBI TT'CG_AA
Cuts at: 2851 2851
Size: 4490
BstYI r'GATC_y
Cuts at: 1 164 1515 1982 2005 2340 2586 3598 3684
Size: 163 1351 467 23 335 246 1012 86
Cuts at: 3684 3696 4042 1
Size: 12 346 449
Fragments arranged by size:
1351 1012 467 449 346 335 246 163
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1104 1104
Size: 4490
BtgI C'CryG_G
Cuts at: 797 797
Size: 4490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2440 2621 2784 2440
Size: 181 163 4146
Fragments arranged by size:
4146 181 163
BtsI GCAGTG_nn'
Cuts at: 297 1733 297
Size: 1436 3054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 126 161 126
Size: 35 4455
DraI TTT'AAA
Cuts at: 1489 1489
Size: 4490
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3054 2196
Size: 858 3632
EaeI y'GGCC_r
Cuts at: 2075 2249 2640 2667 4012 2075
Size: 174 391 27 1345 2553
Fragments arranged by size:
2553 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2075 2075
Size: 4490
EarI CTCTTCn'nnn_
Cuts at: 797 2513 2723 797
Size: 1716 210 2564
Fragments arranged by size:
2564 1716 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2698 3008 3154 4300 2698
Size: 310 146 1146 2888
Fragments arranged by size:
2888 1146 310 146
EcoICRI GAG'CTC
Cuts at: 963 1452 1502 963
Size: 489 50 3951
Fragments arranged by size:
3951 489 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1199 1243 2315 2747 3494 3762 1012
Size: 187 44 1072 432 747 268 1740
Fragments arranged by size:
1740 1072 747 432 268 187 44
EcoNI CCTnn'n_nnAGG
Cuts at: 246 246
Size: 4490
EcoO109I rG'GnC_Cy
Cuts at: 1629 1629
Size: 4490
EcoRI G'AATT_C
Cuts at: 1494 1494
Size: 4490
EcoRV GAT'ATC
Cuts at: 1211 1211
Size: 4490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 222 1470 2509 222
Size: 1248 1039 2203
Fragments arranged by size:
2203 1248 1039
FspI TGC'GCA
Cuts at: 2271 2271
Size: 4490
HaeII r_GCGC'y
Cuts at: 1924 2172 3194 4173 1924
Size: 248 1022 979 2241
Fragments arranged by size:
2241 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 652 684 2332 2362 2364 2394 142
Size: 32 478 32 1648 30 2 30 2238
Fragments arranged by size:
2238 1648 478 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1529 1529
Size: 4490
HindIII A'AGCT_T
Cuts at: 1255 1255
Size: 4490
KasI G'GCGC_C
Cuts at: 1920 2168 1920
Size: 248 4242
KpnI G_GTAC'C
Cuts at: 1510 1510
Size: 4490
MluI A'CGCG_T
Cuts at: 3923 3923
Size: 4490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3161 3345 3161
Size: 184 4306
MscI TGG'CCA
Cuts at: 2251 2251
Size: 4490
MslI CAynn'nnrTG
Cuts at: 836 1310 2479 2606 836
Size: 474 1169 127 2720
Fragments arranged by size:
2720 1169 474 127
MspA1I CmG'CkG
Cuts at: 414 429 521 816 1167 1601 1915 2275 3288
Size: 15 92 295 351 434 314 360 1013
Cuts at: 3288 3533 414
Size: 245 1371
Fragments arranged by size:
1371 1013 434 360 351 314 295 245
92 15
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 4490
NarI GG'CG_CC
Cuts at: 1921 2169 1921
Size: 248 4242
NcoI C'CATG_G
Cuts at: 797 797
Size: 4490
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 4490
NspI r_CATG'y
Cuts at: 188 788 1291 1543 2043 2950 188
Size: 600 503 252 500 907 1728
Fragments arranged by size:
1728 907 600 503 500 252
PciI A'CATG_T
Cuts at: 184 784 1287 2946 184
Size: 600 503 1659 1728
Fragments arranged by size:
1728 1659 600 503
PflMI CCAn_nnn'nTGG
Cuts at: 924 924
Size: 4490
PmeI GTTT'AAAC
Cuts at: 1489 1489
Size: 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4458
PstI C_TGCA'G
Cuts at: 311 422 1391 1537 2222 311
Size: 111 969 146 685 2579
Fragments arranged by size:
2579 969 685 146 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4490
PvuII CAG'CTG
Cuts at: 816 1167 1915 2275 816
Size: 351 748 360 3031
Fragments arranged by size:
3031 748 360 351
RsrII CG'GwC_CG
Cuts at: 2685 2685
Size: 4490
SacI G_AGCT'C
Cuts at: 965 1454 1504 965
Size: 489 50 3951
Fragments arranged by size:
3951 489 50
SalI G'TCGA_C
Cuts at: 1527 1527
Size: 4490
SapI GCTCTTCn'nnn_
Cuts at: 797 2513 2723 797
Size: 1716 210 2564
Fragments arranged by size:
2564 1716 210
SbfI CC_TGCA'GG
Cuts at: 1537 1537
Size: 4490
ScaI AGT'ACT
Cuts at: 4007 4007
Size: 4490
SfcI C'TryA_G
Cuts at: 33 307 418 690 1387 1533 2218 3211 3402
Size: 274 111 272 697 146 685 993 191
Cuts at: 3402 33
Size: 1121
Fragments arranged by size:
1121 993 697 685 274 272 191 146
111
SfoI GGC'GCC
Cuts at: 1922 2170 1922
Size: 248 4242
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
SmaI CCC'GGG
Cuts at: 1512 1512
Size: 4490
SmlI C'TyrA_G
Cuts at: 1645 2560 3052 3314 1645
Size: 915 492 262 2821
Fragments arranged by size:
2821 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4490
SphI G_CATG'C
Cuts at: 1543 2043 1543
Size: 500 3990
StyI C'CwwG_G
Cuts at: 247 324 797 1240 1322 1624 1978 247
Size: 77 473 443 82 302 354 2759
Fragments arranged by size:
2759 473 443 354 302 82 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 761 1186 1235 2073 2206 2665 2874 761
Size: 425 49 838 133 459 209 2377
Fragments arranged by size:
2377 838 459 425 209 133 49
TatI w'GTAC_w
Cuts at: 470 4005 470
Size: 3535 955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 4007 4039 865
Size: 32 3110 32 1316
Fragments arranged by size:
3110 1316 32 32
Tth111I GACn'n_nGTC
Cuts at: 2287 2287
Size: 4490
XbaI T'CTAG_A
Cuts at: 1521 1521
Size: 4490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 921 1223 1985 921
Size: 302 762 3426
Fragments arranged by size:
3426 762 302
XmaI C'CCGG_G
Cuts at: 1510 1510
Size: 4490
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BbsI BfrBI BglI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI