Restriction map for
pF1KE0951
(Circular) (Six-base) MAPSORT of: pF1KE0951.seq Check: 525 from: 1 to: 4532
pF1KE0951 4532 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 1548 1548
Size: 4532
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1450 2357 2789 3536 3804 1358
Size: 92 907 432 747 268 2086
Fragments arranged by size:
2086 907 747 432 268 92
AflII C'TTAA_G
Cuts at: 1084 1084
Size: 4532
AflIII A'CryG_T
Cuts at: 776 1884 2988 3965 776
Size: 1108 1104 977 1343
Fragments arranged by size:
1343 1108 1104 977
AgeI A'CCGG_T
Cuts at: 2920 2920
Size: 4532
AhdI GACnn_n'nnGTC
Cuts at: 780 780
Size: 4532
AlwNI CAG_nnn'CTG
Cuts at: 1837 3404 1837
Size: 1567 2965
ApaI G_GGCC'C
Cuts at: 1257 1257
Size: 4532
ApaLI G'TGCA_C
Cuts at: 3302 3302
Size: 4532
ApoI r'AATT_y
Cuts at: 740 866 1063 1238 1536 3885 4141 740
Size: 126 197 175 298 2349 256 1131
Fragments arranged by size:
2349 1131 298 256 197 175 126
AseI AT'TA_AT
Cuts at: 20 946 20
Size: 926 3606
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 174 1552 174
Size: 1378 3154
BamHI G'GATC_C
Cuts at: 957 1557 957
Size: 600 3932
BanI G'GyrC_C
Cuts at: 123 1356 1548 1962 2210 2245 123
Size: 1233 192 414 248 35 2410
Fragments arranged by size:
2410 1233 414 248 192 35
BanII G_rGCy'C
Cuts at: 1257 1546 2576 1257
Size: 289 1030 3213
Fragments arranged by size:
3213 1030 289
BbeI G_GCGC'C
Cuts at: 1966 2214 1966
Size: 248 4284
BbsI GAAGACnn'nnnn_
Cuts at: 709 1438 709
Size: 729 3803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4498
BciVI GTATCCnnnnn_n'
Cuts at: 1032 2430 3191 4532 1032
Size: 1398 761 1341 1032
Fragments arranged by size:
1398 1341 1032 761
BclI T'GATC_A
Cuts at: 2052 2052
Size: 4532
BglI GCCn_nnn'nGGC
Cuts at: 169 169
Size: 4532
BglII A'GATC_T
Cuts at: 2047 4084 2047
Size: 2037 2495
BlpI GC'TnA_GC
Cuts at: 232 1283 1644 232
Size: 1051 361 3120
Fragments arranged by size:
3120 1051 361
Bme1580I G_kGCm'C
Cuts at: 128 1257 1278 1361 2157 2250 3306 128
Size: 1129 21 83 796 93 1056 1354
Fragments arranged by size:
1354 1129 1056 796 93 83 21
BmrI ACTGGGnnnn_n'
Cuts at: 1744 1825 2160 4304 1744
Size: 81 335 2144 1972
Fragments arranged by size:
2144 1972 335 81
BmtI G_CTAG'C
Cuts at: 1175 1175
Size: 4532
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 614 976 574
Size: 40 362 4130
Fragments arranged by size:
4130 362 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2587 3079 3377 1708
Size: 879 492 298 2863
Fragments arranged by size:
2863 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1172 4236 1172
Size: 3064 1468
BsaAI yAC'GTr
Cuts at: 1885 1885
Size: 4532
BsaBI GATnn'nnATC
Cuts at: 6 2046 4083 6
Size: 2040 2037 455
Fragments arranged by size:
2040 2037 455
BsaHI Gr'CG_yC
Cuts at: 1963 2211 2913 4409 1963
Size: 248 702 1496 2086
Fragments arranged by size:
2086 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1721 2242 2920 3194 3341 3752 1721
Size: 521 678 274 147 411 2501
Fragments arranged by size:
2501 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 877 907 877
Size: 30 4502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 491 1420 1435 1456 491
Size: 929 15 21 3567
Fragments arranged by size:
3567 929 21 15
BseYI C'CCAG_C
Cuts at: 261 1957 2715 3292 3788 261
Size: 1696 758 577 496 1005
Fragments arranged by size:
1696 1005 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1343 1343
Size: 4532
BsiEI CG_ry'CG
Cuts at: 104 2074 2120 3328 104
Size: 1970 46 1208 1308
Fragments arranged by size:
1970 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 698 1546 2324 2514 3306 698
Size: 848 778 190 792 1924
Fragments arranged by size:
1924 848 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 128 698 1257 1278 1361 1546 2157 2250 2324
Size: 570 559 21 83 185 611 93 74
Cuts at: 2324 2514 2576 3306 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 730 611 570 559 190 185 93
83 74 62 21
BspEI T'CCGG_A
Cuts at: 3752 3752
Size: 4532
BspHI T'CATG_A
Cuts at: 698 3708 698
Size: 3010 1522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1582 1796 2098 2479 1582
Size: 214 302 381 3635
Fragments arranged by size:
3635 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1045 2824 2878 4362 4527 50
Size: 995 1779 54 1484 165 55
Fragments arranged by size:
1779 1484 995 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2444 2444
Size: 4532
BsrFI r'CCGG_y
Cuts at: 2530 2711 2920 2530
Size: 181 209 4142
Fragments arranged by size:
4142 209 181
BsrGI T'GTAC_A
Cuts at: 287 1029 287
Size: 742 3790
BssSI C'ACGA_G
Cuts at: 2803 3161 2803
Size: 358 4174
BstAPI GCAn_nnn'nTGC
Cuts at: 1909 1909
Size: 4532
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstEII G'GTnAC_C
Cuts at: 156 156
Size: 4532
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1368 933
Size: 435 4097
BstYI r'GATC_y
Cuts at: 1 957 1557 2024 2047 2382 2628 3640 3726
Size: 956 600 467 23 335 246 1012 86
Cuts at: 3726 3738 4084 1
Size: 12 346 449
Fragments arranged by size:
1012 956 600 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 767 767
Size: 4532
BtgI C'CryG_G
Cuts at: 1000 1143 1000
Size: 143 4389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1262 2482 2663 2826 1262
Size: 1220 181 163 2968
Fragments arranged by size:
2968 1220 181 163
BtsI GCAGTG_nn'
Cuts at: 1373 1775 1373
Size: 402 4130
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 2117 2291 2682 2709 4054 2117
Size: 174 391 27 1345 2595
Fragments arranged by size:
2595 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 156 1119 1334 2555 2765 156
Size: 963 215 1221 210 1923
Fragments arranged by size:
1923 1221 963 215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 126 2740 3050 3196 4342 126
Size: 2614 310 146 1146 316
Fragments arranged by size:
2614 1146 316 310 146
EcoICRI GAG'CTC
Cuts at: 1544 1544
Size: 4532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 614 976 1358 1450 2357 2789 3536 3804
Size: 40 362 382 92 907 432 747 268
Cuts at: 3804 574
Size: 1302
Fragments arranged by size:
1302 907 747 432 382 362 268 92
40
EcoNI CCTnn'n_nnAGG
Cuts at: 1417 1417
Size: 4532
EcoO109I rG'GnC_Cy
Cuts at: 308 1253 1344 1410 1671 308
Size: 945 91 66 261 3169
Fragments arranged by size:
3169 945 261 91 66
EcoRI G'AATT_C
Cuts at: 866 1063 1536 866
Size: 197 473 3862
Fragments arranged by size:
3862 473 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4532
FspI TGC'GCA
Cuts at: 732 2313 732
Size: 1581 2951
HaeII r_GCGC'y
Cuts at: 1966 2214 3236 4215 1966
Size: 248 1022 979 2283
Fragments arranged by size:
2283 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 576 608 766 798 898 930 2374
Size: 32 306 32 158 32 100 32 1444
Cuts at: 2374 2404 2406 2436 238
Size: 30 2 30 2334
Fragments arranged by size:
2334 1444 306 158 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1571 1571
Size: 4532
HindIII A'AGCT_T
Cuts at: 1081 1081
Size: 4532
KasI G'GCGC_C
Cuts at: 1962 2210 1962
Size: 248 4284
KpnI G_GTAC'C
Cuts at: 1552 1552
Size: 4532
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 236 242 3203 3387 236
Size: 6 2961 184 1381
Fragments arranged by size:
2961 1381 184 6
MscI TGG'CCA
Cuts at: 2293 2293
Size: 4532
MslI CAynn'nnrTG
Cuts at: 578 2521 2648 578
Size: 1943 127 2462
Fragments arranged by size:
2462 1943 127
MspA1I CmG'CkG
Cuts at: 1093 1643 1957 2317 3330 3575 1093
Size: 550 314 360 1013 245 2050
Fragments arranged by size:
2050 1013 550 360 314 245
NaeI GCC'GGC
Cuts at: 2713 2713
Size: 4532
NarI GG'CG_CC
Cuts at: 1963 2211 1963
Size: 248 4284
NcoI C'CATG_G
Cuts at: 1143 1143
Size: 4532
NgoMIV G'CCGG_C
Cuts at: 2711 2711
Size: 4532
NheI G'CTAG_C
Cuts at: 1171 1171
Size: 4532
NspI r_CATG'y
Cuts at: 780 1585 2085 2992 780
Size: 805 500 907 2320
Fragments arranged by size:
2320 907 805 500
PasI CC'CwG_GG
Cuts at: 721 721
Size: 4532
PciI A'CATG_T
Cuts at: 776 2988 776
Size: 2212 2320
PfoI T'CCnGG_A
Cuts at: 194 551 1154 194
Size: 357 603 3572
Fragments arranged by size:
3572 603 357
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4500
PpuMI rG'GwC_Cy
Cuts at: 308 308
Size: 4532
PsiI TTA'TAA
Cuts at: 412 412
Size: 4532
PspOMI G'GGCC_C
Cuts at: 1253 1253
Size: 4532
PstI C_TGCA'G
Cuts at: 534 1579 2264 534
Size: 1045 685 2802
Fragments arranged by size:
2802 1045 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 1957 2317 1957
Size: 360 4172
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 1546 1546
Size: 4532
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SanDI GG'GwC_CC
Cuts at: 308 308
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 156 2555 2765 156
Size: 2399 210 1923
Fragments arranged by size:
2399 1923 210
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 4049 4049
Size: 4532
SfcI C'TryA_G
Cuts at: 33 440 530 785 1575 2260 3253 3444 33
Size: 407 90 255 790 685 993 191 1121
Fragments arranged by size:
1121 993 790 685 407 255 191 90
SfoI GGC'GCC
Cuts at: 1964 2212 1964
Size: 248 4284
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 176 1554 176
Size: 1378 3154
SmlI C'TyrA_G
Cuts at: 1084 1687 2602 3094 3356 1084
Size: 603 915 492 262 2260
Fragments arranged by size:
2260 915 603 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 1585 2085 1585
Size: 500 4032
SspI AAT'ATT
Cuts at: 883 883
Size: 4532
StyI C'CwwG_G
Cuts at: 926 1143 1361 1666 2020 926
Size: 217 218 305 354 3438
Fragments arranged by size:
3438 354 305 218 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 144 2115 2248 2707 2916 144
Size: 1971 133 459 209 1760
Fragments arranged by size:
1971 1760 459 209 133
TatI w'GTAC_w
Cuts at: 287 1029 4047 287
Size: 742 3018 772
Fragments arranged by size:
3018 772 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 755 787 4049 4081 755
Size: 32 3262 32 1206
Fragments arranged by size:
3262 1206 32 32
Tth111I GACn'n_nGTC
Cuts at: 934 2329 934
Size: 1395 3137
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2027 2027
Size: 4532
XmaI C'CCGG_G
Cuts at: 174 1552 174
Size: 1378 3154
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI Bpu10I BsiWI BsmI BsmBI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PflMI
PmlI PshAI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI