Restriction map for
pF1KE0954
(Circular) (Six-base) MAPSORT of: pF1KE0954.seq Check: 7298 from: 1 to: 4559
pF1KE0954 4559 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 275 4399 275
Size: 4124 435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2816 3563 3831 2384
Size: 432 747 268 3112
Fragments arranged by size:
3112 747 432 268
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AleI CACnn'nnGTG
Cuts at: 1273 1273
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 1148 1864 3431 1148
Size: 716 1567 2276
Fragments arranged by size:
2276 1567 716
ApaI G_GGCC'C
Cuts at: 299 879 299
Size: 580 3979
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 1563 3912 4168 1563
Size: 2349 256 1954
Fragments arranged by size:
2349 1954 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 697 995 1361 1579 697
Size: 298 366 218 3677
Fragments arranged by size:
3677 366 298 218
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 373 775 799 1278 1343 1575 1989 2237 2272
Size: 402 24 479 65 232 414 248 35
Cuts at: 2272 373
Size: 2660
Fragments arranged by size:
2660 479 414 402 248 232 65 35
24
BanII G_rGCy'C
Cuts at: 176 299 575 879 1433 1573 2603 176
Size: 123 276 304 554 140 1030 2132
Fragments arranged by size:
2132 1030 554 304 276 140 123
BbeI G_GCGC'C
Cuts at: 779 1282 1347 1993 2241 779
Size: 503 65 646 248 3097
Fragments arranged by size:
3097 646 503 248 65
BbvCI CC'TCA_GC
Cuts at: 1234 1234
Size: 4559
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglI GCCn_nnn'nGGC
Cuts at: 112 1252 112
Size: 1140 3419
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 175 461 712 1671 175
Size: 286 251 959 3063
Fragments arranged by size:
3063 959 286 251
Bme1580I G_kGCm'C
Cuts at: 299 879 2184 2277 3333 299
Size: 580 1305 93 1056 1525
Fragments arranged by size:
1525 1305 1056 580 93
BmgBI CAC'GTC
Cuts at: 1170 1170
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 1771 1852 2187 4331 1771
Size: 81 335 2144 1999
Fragments arranged by size:
2144 1999 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1329 2926 1329
Size: 1597 2962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 607
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 1234 1234
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 676 1366 1735 2614 3106 3404 571
Size: 105 690 369 879 492 298 1726
Fragments arranged by size:
1726 879 690 492 369 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 256 4263 256
Size: 4007 552
BsaAI yAC'GTr
Cuts at: 1275 1912 1275
Size: 637 3922
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 776 1204 1279 1344 1542 1990 2238 2940 4436
Size: 428 75 65 198 448 248 702 1496
Cuts at: 4436 776
Size: 899
Fragments arranged by size:
1496 899 702 448 428 248 198 75
65
BsaWI w'CCGG_w
Cuts at: 926 1748 2269 2947 3221 3368 3779 926
Size: 822 521 678 274 147 411 1706
Fragments arranged by size:
1706 822 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1439 1469 1439
Size: 30 4529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 4559
BseYI C'CCAG_C
Cuts at: 324 1030 1411 1417 1984 2742 3319 3815 324
Size: 706 381 6 567 758 577 496 1068
Fragments arranged by size:
1068 758 706 577 567 496 381 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4559
BsiEI CG_ry'CG
Cuts at: 104 116 2101 2147 3355 104
Size: 12 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 176 575 1573 2351 2541 3333 176
Size: 399 998 778 190 792 1402
Fragments arranged by size:
1402 998 792 778 399 190
BsmBI CGTCTCn'nnnn_
Cuts at: 130 130
Size: 4559
Bsp1286I G_dGCh'C
Cuts at: 176 299 575 879 1433 1573 2184 2277 2351
Size: 123 276 304 554 140 611 93 74
Cuts at: 2351 2541 2603 3333 176
Size: 190 62 730 1402
Fragments arranged by size:
1402 730 611 554 304 276 190 140
123 93 74 62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1609 1823 2125 2506 1609
Size: 214 302 381 3662
Fragments arranged by size:
3662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 465 2851 2905 4389 4554 50
Size: 415 2386 54 1484 165 55
Fragments arranged by size:
2386 1484 415 165 55 54
BsrDI GCAATG_nn'
Cuts at: 438 2471 438
Size: 2033 2526
BsrFI r'CCGG_y
Cuts at: 1206 2557 2738 2947 1206
Size: 1351 181 209 2818
Fragments arranged by size:
2818 1351 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 529 1042 529
Size: 513 4046
BstYI r'GATC_y
Cuts at: 1 1584 2051 2074 2409 2655 3667 3753 3765
Size: 1583 467 23 335 246 1012 86 12
Cuts at: 3765 4111 1
Size: 346 449
Fragments arranged by size:
1583 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 409 1509 409
Size: 1100 3459
BtgI C'CryG_G
Cuts at: 107 182 938 1433 1536 107
Size: 75 756 495 103 3130
Fragments arranged by size:
3130 756 495 103 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 300 843 967 1802 300
Size: 543 124 835 3057
Fragments arranged by size:
3057 835 543 124
DraI TTT'AAA
Cuts at: 1558 1558
Size: 4559
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2265 3123 1521
Size: 744 858 2957
Fragments arranged by size:
2957 858 744
EaeI y'GGCC_r
Cuts at: 113 840 2144 2318 2709 2736 4081 113
Size: 727 1304 174 391 27 1345 591
Fragments arranged by size:
1345 1304 727 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2144 113
Size: 2031 2528
EarI CTCTTCn'nnn_
Cuts at: 239 580 2582 2792 239
Size: 341 2002 210 2006
Fragments arranged by size:
2006 2002 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 573 1571 174
Size: 399 998 3162
Fragments arranged by size:
3162 998 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 2384 2816 3563 3831 607
Size: 1777 432 747 268 1335
Fragments arranged by size:
1777 1335 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 412 412
Size: 4559
EcoO109I rG'GnC_Cy
Cuts at: 296 687 875 876 999 1145 1365 1698 296
Size: 391 188 1 123 146 220 333 3157
Fragments arranged by size:
3157 391 333 220 188 146 123 1
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 510 1326 2578 510
Size: 816 1252 2491
Fragments arranged by size:
2491 1252 816
FspI TGC'GCA
Cuts at: 430 835 2340 430
Size: 405 1505 2649
Fragments arranged by size:
2649 1505 405
HaeII r_GCGC'y
Cuts at: 779 1282 1347 1993 2241 3263 4242 779
Size: 503 65 646 248 1022 979 1096
Fragments arranged by size:
1096 1022 979 646 503 248 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 584 616 2401 2431 2433 2463 584
Size: 32 1785 30 2 30 2680
Fragments arranged by size:
2680 1785 32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
KasI G'GCGC_C
Cuts at: 775 1278 1343 1989 2237 775
Size: 503 65 646 248 3097
Fragments arranged by size:
3097 646 503 248 65
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3230 3414 3230
Size: 184 4375
MscI TGG'CCA
Cuts at: 842 2320 842
Size: 1478 3081
MslI CAynn'nnrTG
Cuts at: 1273 2548 2675 1273
Size: 1275 127 3157
Fragments arranged by size:
3157 1275 127
MspA1I CmG'CkG
Cuts at: 184 217 1038 1670 1984 2344 3357 3602 184
Size: 33 821 632 314 360 1013 245 1141
Fragments arranged by size:
1141 1013 821 632 360 314 245 33
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 776 1279 1344 1990 2238 776
Size: 503 65 646 248 3097
Fragments arranged by size:
3097 646 503 248 65
NcoI C'CATG_G
Cuts at: 107 938 107
Size: 831 3728
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 194 894 1612 2112 3019 194
Size: 700 718 500 907 1734
Fragments arranged by size:
1734 907 718 700 500
PasI CC'CwG_GG
Cuts at: 651 669 1054 651
Size: 18 385 4156
Fragments arranged by size:
4156 385 18
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PfoI T'CCnGG_A
Cuts at: 605 1289 605
Size: 684 3875
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PpuMI rG'GwC_Cy
Cuts at: 687 999 1365 687
Size: 312 366 3881
Fragments arranged by size:
3881 366 312
PshAI GACnn'nnGTC
Cuts at: 191 191
Size: 4559
PspOMI G'GGCC_C
Cuts at: 295 875 295
Size: 580 3979
PstI C_TGCA'G
Cuts at: 1157 1606 2291 1157
Size: 449 685 3425
Fragments arranged by size:
3425 685 449
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 217 1984 2344 217
Size: 1767 360 2432
Fragments arranged by size:
2432 1767 360
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 176 575 1573 176
Size: 399 998 3162
Fragments arranged by size:
3162 998 399
SacII CC_GC'GG
Cuts at: 185 185
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SanDI GG'GwC_CC
Cuts at: 1365 1365
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 580 2582 2792 580
Size: 2002 210 2347
Fragments arranged by size:
2347 2002 210
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 1153 1602 2287 3280 3471 33
Size: 1120 449 685 993 191 1121
Fragments arranged by size:
1121 1120 993 685 449 191
SfoI GGC'GCC
Cuts at: 777 1280 1345 1991 2239 777
Size: 503 65 646 248 3097
Fragments arranged by size:
3097 646 503 248 65
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SgrAI Cr'CCGG_yG
Cuts at: 1206 1206
Size: 4559
SmaI CCC'GGG
Cuts at: 997 1363 1581 997
Size: 366 218 3975
Fragments arranged by size:
3975 366 218
SmlI C'TyrA_G
Cuts at: 550 691 1381 1714 2629 3121 3383 550
Size: 141 690 333 915 492 262 1726
Fragments arranged by size:
1726 915 690 492 333 262 141
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StuI AGG'CCT
Cuts at: 1255 1255
Size: 4559
StyI C'CwwG_G
Cuts at: 107 437 938 1693 2047 107
Size: 330 501 755 354 2619
Fragments arranged by size:
2619 755 501 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 1353 2142 2275 2734 2943 754
Size: 599 789 133 459 209 2370
Fragments arranged by size:
2370 789 599 459 209 133
TatI w'GTAC_w
Cuts at: 809 4074 809
Size: 3265 1294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1032 1064 4076 4108 1032
Size: 32 3012 32 1483
Fragments arranged by size:
3012 1483 32 32
Tth111I GACn'n_nGTC
Cuts at: 1003 2356 1003
Size: 1353 3206
XbaI T'CTAG_A
Cuts at: 474 1590 474
Size: 1116 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 810 2054 810
Size: 1244 3315
XmaI C'CCGG_G
Cuts at: 995 1361 1579 995
Size: 366 218 3975
Fragments arranged by size:
3975 366 218
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PsiI PspXI PsrI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI