Restriction map for
pF1KE0960
(Circular) (Six-base) MAPSORT of: pF1KE0960.seq Check: 5562 from: 1 to: 4610
pF1KE0960 4610 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1085 1648 1085
Size: 563 4047
Acc65I G'GTAC_C
Cuts at: 125 1626 125
Size: 1501 3109
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1513 2435 2867 3614 3882 703
Size: 810 922 432 747 268 1431
Fragments arranged by size:
1431 922 810 747 432 268
AflIII A'CryG_T
Cuts at: 821 1962 3066 4043 821
Size: 1141 1104 977 1388
Fragments arranged by size:
1388 1141 1104 977
AgeI A'CCGG_T
Cuts at: 2998 2998
Size: 4610
AleI CACnn'nnGTG
Cuts at: 1072 1072
Size: 4610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1090 1435 1090
Size: 345 4265
AlwNI CAG_nnn'CTG
Cuts at: 1915 3482 1915
Size: 1567 3043
ApaLI G'TGCA_C
Cuts at: 3380 3380
Size: 4610
ApoI r'AATT_y
Cuts at: 1005 1614 3963 4219 1005
Size: 609 2349 256 1396
Fragments arranged by size:
2349 1396 609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 671 1630 671
Size: 959 3651
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 810 843 810
Size: 33 4577
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 125 1626 2040 2288 2323 125
Size: 1501 414 248 35 2412
Fragments arranged by size:
2412 1501 414 248 35
BanII G_rGCy'C
Cuts at: 672 1022 1624 2654 672
Size: 350 602 1030 2628
Fragments arranged by size:
2628 1030 602 350
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbvCI CC'TCA_GC
Cuts at: 548 548
Size: 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4576
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 2130 2130
Size: 4610
BglII A'GATC_T
Cuts at: 2125 4162 2125
Size: 2037 2573
BlpI GC'TnA_GC
Cuts at: 1502 1559 1722 1502
Size: 57 163 4390
Fragments arranged by size:
4390 163 57
Bme1580I G_kGCm'C
Cuts at: 2235 2328 3384 2235
Size: 93 1056 3461
Fragments arranged by size:
3461 1056 93
BmgBI CAC'GTC
Cuts at: 570 570
Size: 4610
BmrI ACTGGGnnnn_n'
Cuts at: 1822 1903 2238 4382 1822
Size: 81 335 2144 2050
Fragments arranged by size:
2144 2050 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1569 1569
Size: 4610
Bpu10I CC'TnA_GC
Cuts at: 548 850 548
Size: 302 4308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 964 1189 1786 2665 3157 3455 748
Size: 216 225 597 879 492 298 1903
Fragments arranged by size:
1903 879 597 492 298 225 216
BsaI GGTCTCn'nnnn_
Cuts at: 4314 4314
Size: 4610
BsaAI yAC'GTr
Cuts at: 1963 1963
Size: 4610
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 240 2041 2289 2991 4487 240
Size: 1801 248 702 1496 363
Fragments arranged by size:
1801 1496 702 363 248
BsaWI w'CCGG_w
Cuts at: 227 302 1799 2320 2998 3272 3419 3830 227
Size: 75 1497 521 678 274 147 411 1007
Fragments arranged by size:
1497 1007 678 521 411 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 147 177 147
Size: 30 4580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 123 486 123
Size: 363 4247
BseYI C'CCAG_C
Cuts at: 1387 2035 2793 3370 3866 1387
Size: 648 758 577 496 2131
Fragments arranged by size:
2131 758 648 577 496
BsiEI CG_ry'CG
Cuts at: 104 303 633 2152 2198 3406 104
Size: 199 330 1519 46 1208 1308
Fragments arranged by size:
1519 1308 1208 330 199 46
BsiHKAI G_wGCw'C
Cuts at: 1022 1069 1118 1155 1624 2402 2592 3384 1022
Size: 47 49 37 469 778 190 792 2248
Fragments arranged by size:
2248 792 778 469 190 49 47 37
BsmI GAATG_Cn'
Cuts at: 396 396
Size: 4610
Bsp1286I G_dGCh'C
Cuts at: 672 1022 1069 1118 1155 1624 2235 2328 2402
Size: 350 47 49 37 469 611 93 74
Cuts at: 2402 2592 2654 3384 672
Size: 190 62 730 1898
Fragments arranged by size:
1898 730 611 469 350 190 93 74
62 49 47 37
BspEI T'CCGG_A
Cuts at: 3830 3830
Size: 4610
BspHI T'CATG_A
Cuts at: 3786 3786
Size: 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 893 1660 1874 2176 2557 893
Size: 767 214 302 381 2946
Fragments arranged by size:
2946 767 381 302 214
BsrBI CCG'CTC
Cuts at: 50 497 2902 2956 4440 4605 50
Size: 447 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 317 2608 2789 2998 317
Size: 2291 181 209 1929
Fragments arranged by size:
2291 1929 209 181
BsrGI T'GTAC_A
Cuts at: 639 639
Size: 4610
BssSI C'ACGA_G
Cuts at: 2881 3239 2881
Size: 358 4252
BstAPI GCAn_nnn'nTGC
Cuts at: 1987 1987
Size: 4610
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstYI r'GATC_y
Cuts at: 1 1292 1635 2102 2125 2460 2706 3718 3804
Size: 1291 343 467 23 335 246 1012 86
Cuts at: 3804 3816 4162 1
Size: 12 346 449
Fragments arranged by size:
1291 1012 467 449 346 343 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 612 612
Size: 4610
BtgI C'CryG_G
Cuts at: 813 813
Size: 4610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 640 2560 2741 2904 640
Size: 1920 181 163 2346
Fragments arranged by size:
2346 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 1062 1101 1853 1062
Size: 39 752 3819
Fragments arranged by size:
3819 752 39
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DraIII CAC_nnn'GTG
Cuts at: 906 906
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 200 2316 3174 200
Size: 2116 858 1636
Fragments arranged by size:
2116 1636 858
EaeI y'GGCC_r
Cuts at: 630 816 2195 2369 2760 2787 4132 630
Size: 186 1379 174 391 27 1345 1108
Fragments arranged by size:
1379 1345 1108 391 186 174 27
EagI C'GGCC_G
Cuts at: 630 2195 630
Size: 1565 3045
EarI CTCTTCn'nnn_
Cuts at: 1057 1252 1468 2633 2843 1057
Size: 195 216 1165 210 2824
Fragments arranged by size:
2824 1165 216 210 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 483 2818 3128 3274 4420 483
Size: 2335 310 146 1146 673
Fragments arranged by size:
2335 1146 673 310 146
EcoICRI GAG'CTC
Cuts at: 1020 1622 1020
Size: 602 4008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1513 1569 2435 2867 3614 3882 703
Size: 810 56 866 432 747 268 1431
Fragments arranged by size:
1431 866 810 747 432 268 56
EcoO109I rG'GnC_Cy
Cuts at: 623 1300 1749 623
Size: 677 449 3484
Fragments arranged by size:
3484 677 449
EcoRI G'AATT_C
Cuts at: 1005 1614 1005
Size: 609 4001
EcoRV GAT'ATC
Cuts at: 1596 1596
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1183 2629 1183
Size: 1446 3164
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 1395 2044 2292 3314 4293 1395
Size: 649 248 1022 979 1712
Fragments arranged by size:
1712 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 584 616 716 748 1229 1261 1284
Size: 32 272 32 100 32 481 32 23
Cuts at: 1284 1316 1366 1398 2452 2482 2484 2514 280
Size: 32 50 32 1054 30 2 30 2376
Fragments arranged by size:
2376 1054 481 272 100 50 32 32
32 32 32 32 30 30 23 2
HincII GTy'rAC
Cuts at: 651 1649 651
Size: 998 3612
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 129 1630 129
Size: 1501 3109
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 3281 3465 323
Size: 2958 184 1468
Fragments arranged by size:
2958 1468 184
MscI TGG'CCA
Cuts at: 818 2371 818
Size: 1553 3057
MslI CAynn'nnrTG
Cuts at: 401 1072 2599 2726 401
Size: 671 1527 127 2285
Fragments arranged by size:
2285 1527 671 127
MspA1I CmG'CkG
Cuts at: 414 927 1072 1387 1419 1721 2035 2395 3408
Size: 513 145 315 32 302 314 360 1013
Cuts at: 3408 3653 414
Size: 245 1371
Fragments arranged by size:
1371 1013 513 360 315 314 302 245
145 32
NaeI GCC'GGC
Cuts at: 2791 2791
Size: 4610
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NcoI C'CATG_G
Cuts at: 813 813
Size: 4610
NgoMIV G'CCGG_C
Cuts at: 2789 2789
Size: 4610
NspI r_CATG'y
Cuts at: 825 1485 1663 2163 3070 825
Size: 660 178 500 907 2365
Fragments arranged by size:
2365 907 660 500 178
PciI A'CATG_T
Cuts at: 821 3066 821
Size: 2245 2365
PfoI T'CCnGG_A
Cuts at: 1312 1312
Size: 4610
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4578
PpuMI rG'GwC_Cy
Cuts at: 623 1300 623
Size: 677 3933
PshAI GACnn'nnGTC
Cuts at: 1586 1586
Size: 4610
PspXI vC'TCGA_Gb
Cuts at: 671 671
Size: 4610
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 412 444 412
Size: 32 4578
PstI C_TGCA'G
Cuts at: 1657 2342 1657
Size: 685 3925
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4610
PvuII CAG'CTG
Cuts at: 927 1072 1387 2035 2395 927
Size: 145 315 648 360 3142
Fragments arranged by size:
3142 648 360 315 145
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 1022 1624 1022
Size: 602 4008
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 1057 1468 2633 2843 1057
Size: 411 1165 210 2824
Fragments arranged by size:
2824 1165 411 210
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 4127 4127
Size: 4610
SfcI C'TryA_G
Cuts at: 33 336 1653 2338 3331 3522 33
Size: 303 1317 685 993 191 1121
Fragments arranged by size:
1317 1121 993 685 303 191
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 1632 1632
Size: 4610
SmlI C'TyrA_G
Cuts at: 671 727 979 1168 1765 2680 3172 3434 671
Size: 56 252 189 597 915 492 262 1847
Fragments arranged by size:
1847 915 597 492 262 252 189 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1485 1663 2163 1485
Size: 178 500 3932
Fragments arranged by size:
3932 500 178
SspI AAT'ATT
Cuts at: 993 1345 993
Size: 352 4258
StyI C'CwwG_G
Cuts at: 813 1514 1744 2098 813
Size: 701 230 354 3325
Fragments arranged by size:
3325 701 354 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1507 2193 2326 2785 2994 1507
Size: 686 133 459 209 3123
Fragments arranged by size:
3123 686 459 209 133
TatI w'GTAC_w
Cuts at: 639 707 4125 639
Size: 68 3418 1124
Fragments arranged by size:
3418 1124 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 4127 4159 147
Size: 32 3948 32 598
Fragments arranged by size:
3948 598 32 32
Tth111I GACn'n_nGTC
Cuts at: 239 2407 239
Size: 2168 2442
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 661 2105 661
Size: 1444 3166
XhoI C'TCGA_G
Cuts at: 671 671
Size: 4610
XmaI C'CCGG_G
Cuts at: 1630 1630
Size: 4610
XmnI GAAnn'nnTTC
Cuts at: 992 1526 992
Size: 534 4076
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BbsI BfrBI BglI BmtI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspOMI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI