Restriction map for
pF1KE0967
(Circular) (Six-base) MAPSORT of: pF1KE0967.seq Check: 9593 from: 1 to: 4652
pF1KE0967 4652 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 812 1690 812
Size: 878 3774
Acc65I G'GTAC_C
Cuts at: 1189 1668 1189
Size: 479 4173
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 422 2477 2909 3656 3924 143
Size: 279 2055 432 747 268 871
Fragments arranged by size:
2055 871 747 432 279 268
AflII C'TTAA_G
Cuts at: 943 943
Size: 4652
AflIII A'CryG_T
Cuts at: 1340 2004 3108 4085 1340
Size: 664 1104 977 1907
Fragments arranged by size:
1907 1104 977 664
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 4652
AhdI GACnn_n'nnGTC
Cuts at: 786 786
Size: 4652
AleI CACnn'nnGTG
Cuts at: 1606 1606
Size: 4652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 305 1466 305
Size: 1161 3491
AlwNI CAG_nnn'CTG
Cuts at: 1957 3524 1957
Size: 1567 3085
ApaLI G'TGCA_C
Cuts at: 3422 3422
Size: 4652
ApoI r'AATT_y
Cuts at: 1144 1656 4005 4261 1144
Size: 512 2349 256 1535
Fragments arranged by size:
2349 1535 512 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 1672 1672
Size: 4652
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4652
BanI G'GyrC_C
Cuts at: 540 874 1189 1668 2082 2330 2365 540
Size: 334 315 479 414 248 35 2827
Fragments arranged by size:
2827 479 414 334 315 248 35
BanII G_rGCy'C
Cuts at: 1666 2696 1666
Size: 1030 3622
BbeI G_GCGC'C
Cuts at: 2086 2334 2086
Size: 248 4404
BbsI GAAGACnn'nnnn_
Cuts at: 1391 1391
Size: 4652
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3311 4652 2550
Size: 761 1341 2550
Fragments arranged by size:
2550 1341 761
BclI T'GATC_A
Cuts at: 902 2172 902
Size: 1270 3382
BfrBI ATG'CAT
Cuts at: 1249 1249
Size: 4652
BglII A'GATC_T
Cuts at: 2167 4204 2167
Size: 2037 2615
BlpI GC'TnA_GC
Cuts at: 1764 1764
Size: 4652
Bme1580I G_kGCm'C
Cuts at: 2277 2370 3426 2277
Size: 93 1056 3503
Fragments arranged by size:
3503 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 729 1864 1945 2280 4424 729
Size: 1135 81 335 2144 957
Fragments arranged by size:
2144 1135 957 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 4652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1193
Size: 4652
Bpu10I CC'TnA_GC
Cuts at: 503 503
Size: 4652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 1828 2707 3199 3497 617
Size: 1211 879 492 298 1772
Fragments arranged by size:
1772 1211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 963 4356 963
Size: 3393 1259
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 890 2166 4203 6
Size: 884 1276 2037 455
Fragments arranged by size:
2037 1276 884 455
BsaHI Gr'CG_yC
Cuts at: 2083 2331 3033 4529 2083
Size: 248 702 1496 2206
Fragments arranged by size:
2206 1496 702 248
BsaWI w'CCGG_w
Cuts at: 459 1841 2362 3040 3314 3461 3872 459
Size: 1382 521 678 274 147 411 1239
Fragments arranged by size:
1382 1239 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1369 1369
Size: 4652
BseYI C'CCAG_C
Cuts at: 2077 2835 3412 3908 2077
Size: 758 577 496 2821
Fragments arranged by size:
2821 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 881
Size: 4652
BsiEI CG_ry'CG
Cuts at: 104 2194 2240 3448 104
Size: 2090 46 1208 1308
Fragments arranged by size:
2090 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1666 2444 2634 3426 1666
Size: 778 190 792 2892
Fragments arranged by size:
2892 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1666 2277 2370 2444 2634 2696 3426 1666
Size: 611 93 74 190 62 730 2892
Fragments arranged by size:
2892 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 459 3872 459
Size: 3413 1239
BspHI T'CATG_A
Cuts at: 915 3828 915
Size: 2913 1739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1062 1702 1916 2218 2599 1062
Size: 640 214 302 381 3115
Fragments arranged by size:
3115 640 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2944 2998 4482 4647 50
Size: 2894 54 1484 165 55
Fragments arranged by size:
2894 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1118 1469 2564 1118
Size: 351 1095 3206
Fragments arranged by size:
3206 1095 351
BsrFI r'CCGG_y
Cuts at: 2650 2831 3040 2650
Size: 181 209 4262
Fragments arranged by size:
4262 209 181
BsrGI T'GTAC_A
Cuts at: 587 587
Size: 4652
BssSI C'ACGA_G
Cuts at: 2923 3281 2923
Size: 358 4294
BstAPI GCAn_nnn'nTGC
Cuts at: 2029 2029
Size: 4652
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstYI r'GATC_y
Cuts at: 1 891 1677 2144 2167 2502 2748 3760 3846
Size: 890 786 467 23 335 246 1012 86
Cuts at: 3846 3858 4204 1
Size: 12 346 449
Fragments arranged by size:
1012 890 786 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 992 1176 1274 107
Size: 885 184 98 3485
Fragments arranged by size:
3485 885 184 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2602 2783 2946 2602
Size: 181 163 4308
Fragments arranged by size:
4308 181 163
BtsI GCAGTG_nn'
Cuts at: 679 1895 679
Size: 1216 3436
DraI TTT'AAA
Cuts at: 1651 1651
Size: 4652
DrdI GACnn_nn'nnGTC
Cuts at: 2358 3216 2358
Size: 858 3794
EaeI y'GGCC_r
Cuts at: 1044 1365 2237 2411 2802 2829 4174 1044
Size: 321 872 174 391 27 1345 1522
Fragments arranged by size:
1522 1345 872 391 321 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4652
EarI CTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2860 3170 3316 4462 2860
Size: 310 146 1146 3050
Fragments arranged by size:
3050 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1664 1664
Size: 4652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 422 1193 2477 2909 3656 3924 143
Size: 279 771 1284 432 747 268 871
Fragments arranged by size:
1284 871 771 747 432 279 268
EcoO109I rG'GnC_Cy
Cuts at: 1791 1791
Size: 4652
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
EcoRV GAT'ATC
Cuts at: 354 354
Size: 4652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4652
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4652
HaeII r_GCGC'y
Cuts at: 2086 2334 3356 4335 2086
Size: 248 1022 979 2403
Fragments arranged by size:
2403 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 772 804 2494 2524 2526 2556 772
Size: 32 1690 30 2 30 2868
Fragments arranged by size:
2868 1690 32 30 30 2
HincII GTy'rAC
Cuts at: 1620 1691 1620
Size: 71 4581
KasI G'GCGC_C
Cuts at: 2082 2330 2082
Size: 248 4404
KpnI G_GTAC'C
Cuts at: 1193 1672 1193
Size: 479 4173
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 652 3323 3507 652
Size: 2671 184 1797
Fragments arranged by size:
2671 1797 184
MscI TGG'CCA
Cuts at: 1046 1367 2413 1046
Size: 321 1046 3285
Fragments arranged by size:
3285 1046 321
MslI CAynn'nnrTG
Cuts at: 1606 2641 2768 1606
Size: 1035 127 3490
Fragments arranged by size:
3490 1035 127
MspA1I CmG'CkG
Cuts at: 1522 1763 2077 2437 3450 3695 1522
Size: 241 314 360 1013 245 2479
Fragments arranged by size:
2479 1013 360 314 245 241
NaeI GCC'GGC
Cuts at: 2833 2833
Size: 4652
NarI GG'CG_CC
Cuts at: 2083 2331 2083
Size: 248 4404
NcoI C'CATG_G
Cuts at: 107 992 1176 1274 107
Size: 885 184 98 3485
Fragments arranged by size:
3485 885 184 98
NgoMIV G'CCGG_C
Cuts at: 2831 2831
Size: 4652
NsiI A_TGCA'T
Cuts at: 1251 1251
Size: 4652
NspI r_CATG'y
Cuts at: 1344 1392 1453 1705 2205 3112 1344
Size: 48 61 252 500 907 2884
Fragments arranged by size:
2884 907 500 252 61 48
PciI A'CATG_T
Cuts at: 1340 3108 1340
Size: 1768 2884
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4620
PstI C_TGCA'G
Cuts at: 686 1167 1320 1699 2384 686
Size: 481 153 379 685 2954
Fragments arranged by size:
2954 685 481 379 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 1522 2077 2437 1522
Size: 555 360 3737
Fragments arranged by size:
3737 555 360
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4652
SacI G_AGCT'C
Cuts at: 1666 1666
Size: 4652
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4442
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4652
ScaI AGT'ACT
Cuts at: 4169 4169
Size: 4652
SfcI C'TryA_G
Cuts at: 33 682 813 1163 1316 1328 1695 2380 3373
Size: 649 131 350 153 12 367 685 993
Cuts at: 3373 3564 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 649 367 350 191 153
131 12
SfoI GGC'GCC
Cuts at: 2084 2332 2084
Size: 248 4404
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 1674 1674
Size: 4652
SmlI C'TyrA_G
Cuts at: 632 943 1807 2722 3214 3476 632
Size: 311 864 915 492 262 1808
Fragments arranged by size:
1808 915 864 492 311 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4652
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
SspI AAT'ATT
Cuts at: 306 767 1569 306
Size: 461 802 3389
Fragments arranged by size:
3389 802 461
StuI AGG'CCT
Cuts at: 1422 1422
Size: 4652
StyI C'CwwG_G
Cuts at: 107 536 992 1176 1274 1786 2140 107
Size: 429 456 184 98 512 354 2619
Fragments arranged by size:
2619 512 456 429 354 184 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 2235 2368 2827 3036 573
Size: 1662 133 459 209 2189
Fragments arranged by size:
2189 1662 459 209 133
TatI w'GTAC_w
Cuts at: 587 618 1309 4167 587
Size: 31 691 2858 1072
Fragments arranged by size:
2858 1072 691 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 363 395 474 506 4169 4201 363
Size: 32 79 32 3663 32 814
Fragments arranged by size:
3663 814 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 885 1183 2147 885
Size: 298 964 3390
Fragments arranged by size:
3390 964 298
XmaI C'CCGG_G
Cuts at: 1672 1672
Size: 4652
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI