Restriction map for
pF1KE0968
(Circular) (Six-base) MAPSORT of: pF1KE0968.seq Check: 9155 from: 1 to: 4673
pF1KE0968 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 890 1556 1711 890
Size: 666 155 3852
Fragments arranged by size:
3852 666 155
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 1649 4513 1649
Size: 2864 1809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 406 574 696 2498 2930 3677 3945 116
Size: 290 168 122 1802 432 747 268 844
Fragments arranged by size:
1802 844 747 432 290 268 168 122
AflIII A'CryG_T
Cuts at: 893 965 2025 3129 4106 893
Size: 72 1060 1104 977 1460
Fragments arranged by size:
1460 1104 1060 977 72
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AleI CACnn'nnGTG
Cuts at: 1198 1198
Size: 4673
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 283
Size: 32 4641
AlwNI CAG_nnn'CTG
Cuts at: 543 1350 1978 3545 543
Size: 807 628 1567 1671
Fragments arranged by size:
1671 1567 807 628
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 343 1677 4026 4282 343
Size: 1334 2349 256 734
Fragments arranged by size:
2349 1334 734 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1021 1416 1693 1021
Size: 395 277 4001
Fragments arranged by size:
4001 395 277
BamHI G'GATC_C
Cuts at: 168 1698 168
Size: 1530 3143
BanI G'GyrC_C
Cuts at: 1689 2103 2351 2386 1689
Size: 414 248 35 3976
Fragments arranged by size:
3976 414 248 35
BanII G_rGCy'C
Cuts at: 125 662 1687 2717 125
Size: 537 1025 1030 2081
Fragments arranged by size:
2081 1030 1025 537
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4639
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 399 1500 2193 399
Size: 1101 693 2879
Fragments arranged by size:
2879 1101 693
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 2298 2391 3447 2298
Size: 93 1056 3524
Fragments arranged by size:
3524 1056 93
BmgBI CAC'GTC
Cuts at: 954 954
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 1885 1966 2301 4445 1885
Size: 81 335 2144 2113
Fragments arranged by size:
2144 2113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1086 1501 448
Size: 638 415 3620
Fragments arranged by size:
3620 638 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 784 1276 1309 1621 1849 2728 3220 3518
Size: 675 492 33 312 228 879 492 298
Cuts at: 3518 109
Size: 1264
Fragments arranged by size:
1264 879 675 492 492 312 298 228
33
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 896 2026 896
Size: 1130 3543
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 283
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 196
Size: 4673
BseYI C'CCAG_C
Cuts at: 1065 2098 2856 3433 3929 1065
Size: 1033 758 577 496 1809
Fragments arranged by size:
1809 1033 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 662 1687 2465 2655 3447 125
Size: 537 1025 778 190 792 1351
Fragments arranged by size:
1351 1025 792 778 537 190
BsmI GAATG_Cn'
Cuts at: 1491 1491
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 125 662 1687 2298 2391 2465 2655 2717 3447
Size: 537 1025 611 93 74 190 62 730
Cuts at: 3447 125
Size: 1351
Fragments arranged by size:
1351 1025 730 611 537 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4503 4668 50
Size: 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 404 2585 404
Size: 2181 2492
BsrFI r'CCGG_y
Cuts at: 111 908 1081 2671 2852 3061 111
Size: 797 173 1590 181 209 1723
Fragments arranged by size:
1723 1590 797 209 181 173
BsrGI T'GTAC_A
Cuts at: 998 998
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 471 1227 471
Size: 756 3917
BstYI r'GATC_y
Cuts at: 1 168 557 1698 2165 2188 2523 2769 3781
Size: 167 389 1141 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1141 1012 467 449 389 346 335 246
167 86 23 12
BstZ17I GTA'TAC
Cuts at: 891 891
Size: 4673
Bsu36I CC'TnA_GG
Cuts at: 1351 1351
Size: 4673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 461 956 2258 2432 2823 2850 4195 461
Size: 495 1302 174 391 27 1345 939
Fragments arranged by size:
1345 1302 939 495 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 517 1271 2696 2906 517
Size: 754 1425 210 2284
Fragments arranged by size:
2284 1425 754 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1006 1606 2881 3191 3337 4483 1006
Size: 600 1275 310 146 1146 1196
Fragments arranged by size:
1275 1196 1146 600 310 146
EcoICRI GAG'CTC
Cuts at: 123 660 1685 123
Size: 537 1025 3111
Fragments arranged by size:
3111 1025 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 406 448 574 696 1086 1501 2498 2930
Size: 290 42 126 122 390 415 997 432
Cuts at: 2930 3677 3945 116
Size: 747 268 844
Fragments arranged by size:
997 844 747 432 415 390 290 268
126 122 42
EcoO109I rG'GnC_Cy
Cuts at: 228 390 446 540 1188 1287 1340 1812 228
Size: 162 56 94 648 99 53 472 3089
Fragments arranged by size:
3089 648 472 162 99 94 56 53
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 2692 465
Size: 2227 2446
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 735 2107 2355 3377 4356 735
Size: 1372 248 1022 979 1052
Fragments arranged by size:
1372 1052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 847 879 2515 2545 2547 2577 172
Size: 32 643 32 1636 30 2 30 2268
Fragments arranged by size:
2268 1636 643 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
HindIII A'AGCT_T
Cuts at: 235 235
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3344 3528 3344
Size: 184 4489
MscI TGG'CCA
Cuts at: 463 958 2434 463
Size: 495 1476 2702
Fragments arranged by size:
2702 1476 495
MslI CAynn'nnrTG
Cuts at: 964 1060 1198 1496 2662 2789 964
Size: 96 138 298 1166 127 2848
Fragments arranged by size:
2848 1166 298 138 127 96
MspA1I CmG'CkG
Cuts at: 220 252 1044 1542 1582 1784 2098 2458 3471
Size: 32 792 498 40 202 314 360 1013
Cuts at: 3471 3716 220
Size: 245 1177
Fragments arranged by size:
1177 1013 792 498 360 314 245 202
40 32
NaeI GCC'GGC
Cuts at: 113 1083 2854 113
Size: 970 1771 1932
Fragments arranged by size:
1932 1771 970
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NdeI CA'TA_TG
Cuts at: 261 611 261
Size: 350 4323
NgoMIV G'CCGG_C
Cuts at: 111 1081 2852 111
Size: 970 1771 1932
Fragments arranged by size:
1932 1771 970
NspI r_CATG'y
Cuts at: 969 1300 1486 1726 2226 3133 969
Size: 331 186 240 500 907 2509
Fragments arranged by size:
2509 907 500 331 240 186
PciI A'CATG_T
Cuts at: 965 3129 965
Size: 2164 2509
PfoI T'CCnGG_A
Cuts at: 560 1102 1226 560
Size: 542 124 4007
Fragments arranged by size:
4007 542 124
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 283 315 3812 3844 283
Size: 32 3497 32 1112
Fragments arranged by size:
3497 1112 32 32
PpuMI rG'GwC_Cy
Cuts at: 390 446 1188 390
Size: 56 742 3875
Fragments arranged by size:
3875 742 56
PspXI vC'TCGA_Gb
Cuts at: 1416 1416
Size: 4673
PstI C_TGCA'G
Cuts at: 1720 2405 1720
Size: 685 3988
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 252 1044 1542 1582 2098 2458 252
Size: 792 498 40 516 360 2467
Fragments arranged by size:
2467 792 516 498 360 40
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 125 662 1687 125
Size: 537 1025 3111
Fragments arranged by size:
3111 1025 537
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 1451 1716 2401 3394 3585 33
Size: 1418 265 685 993 191 1121
Fragments arranged by size:
1418 1121 993 685 265 191
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1023 1695 1023
Size: 672 4001
SmlI C'TyrA_G
Cuts at: 124 799 1291 1324 1416 1636 1828 2743 3235
Size: 675 492 33 92 220 192 915 492
Cuts at: 3235 3497 124
Size: 262 1300
Fragments arranged by size:
1300 915 675 492 492 262 220 192
92 33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1300 1726 2226 1300
Size: 426 500 3747
Fragments arranged by size:
3747 500 426
StyI C'CwwG_G
Cuts at: 173 1184 1807 2161 173
Size: 1011 623 354 2685
Fragments arranged by size:
2685 1011 623 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2256 2389 2848 3057 2256
Size: 133 459 209 3872
Fragments arranged by size:
3872 459 209 133
TatI w'GTAC_w
Cuts at: 998 4188 998
Size: 3190 1483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XhoI C'TCGA_G
Cuts at: 1416 1416
Size: 4673
XmaI C'CCGG_G
Cuts at: 1021 1693 1021
Size: 672 4001
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstEII BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI