(Circular) (Six-base) MAPSORT of: pF1KE0968.seq Check: 9155 from: 1 to: 4673 pF1KE0968 4673 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AccI GT'mk_AC Cuts at: 890 1556 1711 890 Size: 666 155 3852 Fragments arranged by size: 3852 666 155 Acc65I G'GTAC_C Cuts at: 1689 1689 Size: 4673 AclI AA'CG_TT Cuts at: 1649 4513 1649 Size: 2864 1809 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 116 406 574 696 2498 2930 3677 3945 116 Size: 290 168 122 1802 432 747 268 844 Fragments arranged by size: 1802 844 747 432 290 268 168 122 AflIII A'CryG_T Cuts at: 893 965 2025 3129 4106 893 Size: 72 1060 1104 977 1460 Fragments arranged by size: 1460 1104 1060 977 72 AgeI A'CCGG_T Cuts at: 3061 3061 Size: 4673 AleI CACnn'nnGTG Cuts at: 1198 1198 Size: 4673 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 283 315 283 Size: 32 4641 AlwNI CAG_nnn'CTG Cuts at: 543 1350 1978 3545 543 Size: 807 628 1567 1671 Fragments arranged by size: 1671 1567 807 628 ApaLI G'TGCA_C Cuts at: 3443 3443 Size: 4673 ApoI r'AATT_y Cuts at: 343 1677 4026 4282 343 Size: 1334 2349 256 734 Fragments arranged by size: 2349 1334 734 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4673 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4673 AvaI C'yCGr_G Cuts at: 1021 1416 1693 1021 Size: 395 277 4001 Fragments arranged by size: 4001 395 277 BamHI G'GATC_C Cuts at: 168 1698 168 Size: 1530 3143 BanI G'GyrC_C Cuts at: 1689 2103 2351 2386 1689 Size: 414 248 35 3976 Fragments arranged by size: 3976 414 248 35 BanII G_rGCy'C Cuts at: 125 662 1687 2717 125 Size: 537 1025 1030 2081 Fragments arranged by size: 2081 1030 1025 537 BbeI G_GCGC'C Cuts at: 2107 2355 2107 Size: 248 4425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4639 BciVI GTATCCnnnnn_n' Cuts at: 2571 3332 4673 2571 Size: 761 1341 2571 Fragments arranged by size: 2571 1341 761 BclI T'GATC_A Cuts at: 399 1500 2193 399 Size: 1101 693 2879 Fragments arranged by size: 2879 1101 693 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 4673 BglII A'GATC_T Cuts at: 2188 4225 2188 Size: 2037 2636 BlpI GC'TnA_GC Cuts at: 1785 1785 Size: 4673 Bme1580I G_kGCm'C Cuts at: 2298 2391 3447 2298 Size: 93 1056 3524 Fragments arranged by size: 3524 1056 93 BmgBI CAC'GTC Cuts at: 954 954 Size: 4673 BmrI ACTGGGnnnn_n' Cuts at: 1885 1966 2301 4445 1885 Size: 81 335 2144 2113 Fragments arranged by size: 2144 2113 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3040 3040 Size: 4673 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 448 1086 1501 448 Size: 638 415 3620 Fragments arranged by size: 3620 638 415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 109 784 1276 1309 1621 1849 2728 3220 3518 Size: 675 492 33 312 228 879 492 298 Cuts at: 3518 109 Size: 1264 Fragments arranged by size: 1264 879 675 492 492 312 298 228 33 BsaI GGTCTCn'nnnn_ Cuts at: 4377 4377 Size: 4673 BsaAI yAC'GTr Cuts at: 896 2026 896 Size: 1130 3543 BsaBI GATnn'nnATC Cuts at: 6 2187 4224 6 Size: 2181 2037 455 Fragments arranged by size: 2181 2037 455 BsaHI Gr'CG_yC Cuts at: 2104 2352 3054 4550 2104 Size: 248 702 1496 2227 Fragments arranged by size: 2227 1496 702 248 BsaWI w'CCGG_w Cuts at: 1862 2383 3061 3335 3482 3893 1862 Size: 521 678 274 147 411 2642 Fragments arranged by size: 2642 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 283 313 283 Size: 30 4643 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 196 196 Size: 4673 BseYI C'CCAG_C Cuts at: 1065 2098 2856 3433 3929 1065 Size: 1033 758 577 496 1809 Fragments arranged by size: 1809 1033 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2215 2261 3469 104 Size: 2111 46 1208 1308 Fragments arranged by size: 2111 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 125 662 1687 2465 2655 3447 125 Size: 537 1025 778 190 792 1351 Fragments arranged by size: 1351 1025 792 778 537 190 BsmI GAATG_Cn' Cuts at: 1491 1491 Size: 4673 Bsp1286I G_dGCh'C Cuts at: 125 662 1687 2298 2391 2465 2655 2717 3447 Size: 537 1025 611 93 74 190 62 730 Cuts at: 3447 125 Size: 1351 Fragments arranged by size: 1351 1025 730 611 537 190 93 74 62 BspEI T'CCGG_A Cuts at: 3893 3893 Size: 4673 BspHI T'CATG_A Cuts at: 3849 3849 Size: 4673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1723 1937 2239 2620 1723 Size: 214 302 381 3776 Fragments arranged by size: 3776 381 302 214 BsrBI CCG'CTC Cuts at: 50 2965 3019 4503 4668 50 Size: 2915 54 1484 165 55 Fragments arranged by size: 2915 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 404 2585 404 Size: 2181 2492 BsrFI r'CCGG_y Cuts at: 111 908 1081 2671 2852 3061 111 Size: 797 173 1590 181 209 1723 Fragments arranged by size: 1723 1590 797 209 181 173 BsrGI T'GTAC_A Cuts at: 998 998 Size: 4673 BssSI C'ACGA_G Cuts at: 2944 3302 2944 Size: 358 4315 BstAPI GCAn_nnn'nTGC Cuts at: 2050 2050 Size: 4673 BstBI TT'CG_AA Cuts at: 3034 3034 Size: 4673 BstXI CCAn_nnnn'nTGG Cuts at: 471 1227 471 Size: 756 3917 BstYI r'GATC_y Cuts at: 1 168 557 1698 2165 2188 2523 2769 3781 Size: 167 389 1141 467 23 335 246 1012 Cuts at: 3781 3867 3879 4225 1 Size: 86 12 346 449 Fragments arranged by size: 1141 1012 467 449 389 346 335 246 167 86 23 12 BstZ17I GTA'TAC Cuts at: 891 891 Size: 4673 Bsu36I CC'TnA_GG Cuts at: 1351 1351 Size: 4673 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2623 2804 2967 2623 Size: 181 163 4329 Fragments arranged by size: 4329 181 163 BtsI GCAGTG_nn' Cuts at: 1916 1916 Size: 4673 DraI TTT'AAA Cuts at: 1672 1672 Size: 4673 DrdI GACnn_nn'nnGTC Cuts at: 2379 3237 2379 Size: 858 3815 EaeI y'GGCC_r Cuts at: 461 956 2258 2432 2823 2850 4195 461 Size: 495 1302 174 391 27 1345 939 Fragments arranged by size: 1345 1302 939 495 391 174 27 EagI C'GGCC_G Cuts at: 2258 2258 Size: 4673 EarI CTCTTCn'nnn_ Cuts at: 517 1271 2696 2906 517 Size: 754 1425 210 2284 Fragments arranged by size: 2284 1425 754 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1006 1606 2881 3191 3337 4483 1006 Size: 600 1275 310 146 1146 1196 Fragments arranged by size: 1275 1196 1146 600 310 146 EcoICRI GAG'CTC Cuts at: 123 660 1685 123 Size: 537 1025 3111 Fragments arranged by size: 3111 1025 537 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 116 406 448 574 696 1086 1501 2498 2930 Size: 290 42 126 122 390 415 997 432 Cuts at: 2930 3677 3945 116 Size: 747 268 844 Fragments arranged by size: 997 844 747 432 415 390 290 268 126 122 42 EcoO109I rG'GnC_Cy Cuts at: 228 390 446 540 1188 1287 1340 1812 228 Size: 162 56 94 648 99 53 472 3089 Fragments arranged by size: 3089 648 472 162 99 94 56 53 EcoRI G'AATT_C Cuts at: 1677 1677 Size: 4673 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 465 2692 465 Size: 2227 2446 FspI TGC'GCA Cuts at: 2454 2454 Size: 4673 HaeII r_GCGC'y Cuts at: 735 2107 2355 3377 4356 735 Size: 1372 248 1022 979 1052 Fragments arranged by size: 1372 1052 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 172 204 847 879 2515 2545 2547 2577 172 Size: 32 643 32 1636 30 2 30 2268 Fragments arranged by size: 2268 1636 643 32 32 30 30 2 HincII GTy'rAC Cuts at: 1712 1712 Size: 4673 HindIII A'AGCT_T Cuts at: 235 235 Size: 4673 KasI G'GCGC_C Cuts at: 2103 2351 2103 Size: 248 4425 KpnI G_GTAC'C Cuts at: 1693 1693 Size: 4673 MluI A'CGCG_T Cuts at: 4106 4106 Size: 4673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3344 3528 3344 Size: 184 4489 MscI TGG'CCA Cuts at: 463 958 2434 463 Size: 495 1476 2702 Fragments arranged by size: 2702 1476 495 MslI CAynn'nnrTG Cuts at: 964 1060 1198 1496 2662 2789 964 Size: 96 138 298 1166 127 2848 Fragments arranged by size: 2848 1166 298 138 127 96 MspA1I CmG'CkG Cuts at: 220 252 1044 1542 1582 1784 2098 2458 3471 Size: 32 792 498 40 202 314 360 1013 Cuts at: 3471 3716 220 Size: 245 1177 Fragments arranged by size: 1177 1013 792 498 360 314 245 202 40 32 NaeI GCC'GGC Cuts at: 113 1083 2854 113 Size: 970 1771 1932 Fragments arranged by size: 1932 1771 970 NarI GG'CG_CC Cuts at: 2104 2352 2104 Size: 248 4425 NdeI CA'TA_TG Cuts at: 261 611 261 Size: 350 4323 NgoMIV G'CCGG_C Cuts at: 111 1081 2852 111 Size: 970 1771 1932 Fragments arranged by size: 1932 1771 970 NspI r_CATG'y Cuts at: 969 1300 1486 1726 2226 3133 969 Size: 331 186 240 500 907 2509 Fragments arranged by size: 2509 907 500 331 240 186 PciI A'CATG_T Cuts at: 965 3129 965 Size: 2164 2509 PfoI T'CCnGG_A Cuts at: 560 1102 1226 560 Size: 542 124 4007 Fragments arranged by size: 4007 542 124 PmeI GTTT'AAAC Cuts at: 1672 1672 Size: 4673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 283 315 3812 3844 283 Size: 32 3497 32 1112 Fragments arranged by size: 3497 1112 32 32 PpuMI rG'GwC_Cy Cuts at: 390 446 1188 390 Size: 56 742 3875 Fragments arranged by size: 3875 742 56 PspXI vC'TCGA_Gb Cuts at: 1416 1416 Size: 4673 PstI C_TGCA'G Cuts at: 1720 2405 1720 Size: 685 3988 PvuI CG_AT'CG Cuts at: 104 104 Size: 4673 PvuII CAG'CTG Cuts at: 252 1044 1542 1582 2098 2458 252 Size: 792 498 40 516 360 2467 Fragments arranged by size: 2467 792 516 498 360 40 RsrII CG'GwC_CG Cuts at: 2868 2868 Size: 4673 SacI G_AGCT'C Cuts at: 125 662 1687 125 Size: 537 1025 3111 Fragments arranged by size: 3111 1025 537 SalI G'TCGA_C Cuts at: 1710 1710 Size: 4673 SapI GCTCTTCn'nnn_ Cuts at: 2696 2906 2696 Size: 210 4463 SbfI CC_TGCA'GG Cuts at: 1720 1720 Size: 4673 ScaI AGT'ACT Cuts at: 4190 4190 Size: 4673 SfcI C'TryA_G Cuts at: 33 1451 1716 2401 3394 3585 33 Size: 1418 265 685 993 191 1121 Fragments arranged by size: 1418 1121 993 685 265 191 SfoI GGC'GCC Cuts at: 2105 2353 2105 Size: 248 4425 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4673 SmaI CCC'GGG Cuts at: 1023 1695 1023 Size: 672 4001 SmlI C'TyrA_G Cuts at: 124 799 1291 1324 1416 1636 1828 2743 3235 Size: 675 492 33 92 220 192 915 492 Cuts at: 3235 3497 124 Size: 262 1300 Fragments arranged by size: 1300 915 675 492 492 262 220 192 92 33 SpeI A'CTAG_T Cuts at: 67 67 Size: 4673 SphI G_CATG'C Cuts at: 1300 1726 2226 1300 Size: 426 500 3747 Fragments arranged by size: 3747 500 426 StyI C'CwwG_G Cuts at: 173 1184 1807 2161 173 Size: 1011 623 354 2685 Fragments arranged by size: 2685 1011 623 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2256 2389 2848 3057 2256 Size: 133 459 209 3872 Fragments arranged by size: 3872 459 209 133 TatI w'GTAC_w Cuts at: 998 4188 998 Size: 3190 1483 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4190 4222 4190 Size: 32 4641 Tth111I GACn'n_nGTC Cuts at: 2470 2470 Size: 4673 XbaI T'CTAG_A Cuts at: 1704 1704 Size: 4673 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2168 2168 Size: 4673 XhoI C'TCGA_G Cuts at: 1416 1416 Size: 4673 XmaI C'CCGG_G Cuts at: 1021 1693 1021 Size: 672 4001 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI