Restriction map for
pF1KE0975
(Circular) (Six-base) MAPSORT of: pF1KE0975.seq Check: 8466 from: 1 to: 4745
pF1KE0975 4745 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1217 1721 1217
Size: 504 4241
AatII G_ACGT'C
Cuts at: 443 443
Size: 4745
AccI GT'mk_AC
Cuts at: 1783 1783
Size: 4745
Acc65I G'GTAC_C
Cuts at: 653 1761 653
Size: 1108 3637
AclI AA'CG_TT
Cuts at: 1334 4585 1334
Size: 3251 1494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3002 3749 4017 2570
Size: 432 747 268 3298
Fragments arranged by size:
3298 747 432 268
AflIII A'CryG_T
Cuts at: 707 2097 3201 4178 707
Size: 1390 1104 977 1274
Fragments arranged by size:
1390 1274 1104 977
AgeI A'CCGG_T
Cuts at: 3133 3133
Size: 4745
AleI CACnn'nnGTG
Cuts at: 118 118
Size: 4745
AlwNI CAG_nnn'CTG
Cuts at: 471 900 1002 1509 2050 3617 471
Size: 429 102 507 541 1567 1599
Fragments arranged by size:
1599 1567 541 507 429 102
ApaI G_GGCC'C
Cuts at: 1180 1180
Size: 4745
ApaLI G'TGCA_C
Cuts at: 111 508 3515 111
Size: 397 3007 1341
Fragments arranged by size:
3007 1341 397
ApoI r'AATT_y
Cuts at: 1749 4098 4354 1749
Size: 2349 256 2140
Fragments arranged by size:
2349 2140 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4745
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
AvaI C'yCGr_G
Cuts at: 163 495 1073 1164 1765 163
Size: 332 578 91 601 3143
Fragments arranged by size:
3143 601 578 332 91
AvrII C'CTAG_G
Cuts at: 1377 1377
Size: 4745
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 637 670 637
Size: 33 4712
BamHI G'GATC_C
Cuts at: 1770 1770
Size: 4745
BanI G'GyrC_C
Cuts at: 653 1159 1633 1645 1730 1761 2175 2423 2458
Size: 506 474 12 85 31 414 248 35
Cuts at: 2458 653
Size: 2940
Fragments arranged by size:
2940 506 474 414 248 85 35 31
12
BanII G_rGCy'C
Cuts at: 1180 1759 2789 1180
Size: 579 1030 3136
Fragments arranged by size:
3136 1030 579
BbeI G_GCGC'C
Cuts at: 2179 2427 2179
Size: 248 4497
BbvCI CC'TCA_GC
Cuts at: 1232 1232
Size: 4745
BciVI GTATCCnnnnn_n'
Cuts at: 736 2643 3404 4745 736
Size: 1907 761 1341 736
Fragments arranged by size:
1907 1341 761 736
BclI T'GATC_A
Cuts at: 2265 2265
Size: 4745
BglI GCCn_nnn'nGGC
Cuts at: 1504 1504
Size: 4745
BglII A'GATC_T
Cuts at: 2260 4297 2260
Size: 2037 2708
BlpI GC'TnA_GC
Cuts at: 1857 1857
Size: 4745
Bme1580I G_kGCm'C
Cuts at: 115 503 512 1053 1080 1180 1638 1733 2370
Size: 388 9 541 27 100 458 95 637
Cuts at: 2370 2463 3519 115
Size: 93 1056 1341
Fragments arranged by size:
1341 1056 637 541 458 388 100 95
93 27 9
BmgBI CAC'GTC
Cuts at: 342 770 342
Size: 428 4317
BmrI ACTGGGnnnn_n'
Cuts at: 1714 1957 2038 2373 4517 1714
Size: 243 81 335 2144 1942
Fragments arranged by size:
2144 1942 335 243 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 3112 1206
Size: 1906 2839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 808 820 1159 1744 283
Size: 525 12 339 585 3284
Fragments arranged by size:
3284 585 525 339 12
Bpu10I CC'TnA_GC
Cuts at: 846 1232 1490 846
Size: 386 258 4101
Fragments arranged by size:
4101 386 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1921 2800 3292 3590 1921
Size: 879 492 298 3076
Fragments arranged by size:
3076 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 744 796 4449 744
Size: 52 3653 1040
Fragments arranged by size:
3653 1040 52
BsaAI yAC'GTr
Cuts at: 447 639 1017 2098 447
Size: 192 378 1081 3094
Fragments arranged by size:
3094 1081 378 192
BsaBI GATnn'nnATC
Cuts at: 6 2259 4296 6
Size: 2253 2037 455
Fragments arranged by size:
2253 2037 455
BsaHI Gr'CG_yC
Cuts at: 440 2176 2424 3126 4622 440
Size: 1736 248 702 1496 563
Fragments arranged by size:
1736 1496 702 563 248
BsaWI w'CCGG_w
Cuts at: 137 203 416 663 1584 1934 2455 3133 3407
Size: 66 213 247 921 350 521 678 274
Cuts at: 3407 3554 3965 137
Size: 147 411 917
Fragments arranged by size:
921 917 678 521 411 350 274 247
213 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 436 466 198
Size: 30 208 30 4477
Fragments arranged by size:
4477 208 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 838 286
Size: 552 4193
BseYI C'CCAG_C
Cuts at: 876 1036 2170 2928 3505 4001 876
Size: 160 1134 758 577 496 1620
Fragments arranged by size:
1620 1134 758 577 496 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 853 1039 1195 1276 1343 537
Size: 316 186 156 81 67 3939
Fragments arranged by size:
3939 316 186 156 81 67
BsiEI CG_ry'CG
Cuts at: 104 1602 2287 2333 3541 104
Size: 1498 685 46 1208 1308
Fragments arranged by size:
1498 1308 1208 685 46
BsiHKAI G_wGCw'C
Cuts at: 115 512 539 663 1310 1759 2537 2727 3519
Size: 397 27 124 647 449 778 190 792
Cuts at: 3519 115
Size: 1341
Fragments arranged by size:
1341 792 778 647 449 397 190 124
27
Bsp1286I G_dGCh'C
Cuts at: 115 503 512 539 663 1053 1080 1180 1310
Size: 388 9 27 124 390 27 100 130
Cuts at: 1310 1638 1733 1759 2370 2463 2537 2727 2789
Size: 328 95 26 611 93 74 190 62
Cuts at: 2789 3519 115
Size: 730 1341
Fragments arranged by size:
1341 730 611 390 388 328 190 130
124 100 95 93 74 62 27 27
26 9
BspEI T'CCGG_A
Cuts at: 137 416 1584 3965 137
Size: 279 1168 2381 917
Fragments arranged by size:
2381 1168 917 279
BspHI T'CATG_A
Cuts at: 3921 3921
Size: 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1217 1721 1795 2009 2311 2692 1217
Size: 504 74 214 302 381 3270
Fragments arranged by size:
3270 504 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 587 1354 3037 3091 4575 4740 50
Size: 537 767 1683 54 1484 165 55
Fragments arranged by size:
1683 1484 767 537 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2657 2657
Size: 4745
BsrFI r'CCGG_y
Cuts at: 2743 2924 3133 2743
Size: 181 209 4355
Fragments arranged by size:
4355 209 181
BssSI C'ACGA_G
Cuts at: 1052 1309 3016 3374 1052
Size: 257 1707 358 2423
Fragments arranged by size:
2423 1707 358 257
BstAPI GCAn_nnn'nTGC
Cuts at: 507 2122 507
Size: 1615 3130
BstBI TT'CG_AA
Cuts at: 3106 3106
Size: 4745
BstXI CCAn_nnnn'nTGG
Cuts at: 307 307
Size: 4745
BstYI r'GATC_y
Cuts at: 1 1770 2237 2260 2595 2841 3853 3939 3951
Size: 1769 467 23 335 246 1012 86 12
Cuts at: 3951 4297 1
Size: 346 449
Fragments arranged by size:
1769 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 605 614 949 605
Size: 9 335 4401
Fragments arranged by size:
4401 335 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2695 2876 3039 2695
Size: 181 163 4401
Fragments arranged by size:
4401 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1000 1029 1988 203
Size: 797 29 959 2960
Fragments arranged by size:
2960 959 797 29
DraI TTT'AAA
Cuts at: 1744 1744
Size: 4745
DraIII CAC_nnn'GTG
Cuts at: 507 1737 507
Size: 1230 3515
DrdI GACnn_nn'nnGTC
Cuts at: 2451 3309 2451
Size: 858 3887
EaeI y'GGCC_r
Cuts at: 224 641 886 1496 1550 1599 2330 2504 2895
Size: 417 245 610 54 49 731 174 391
Cuts at: 2895 2922 4267 224
Size: 27 1345 702
Fragments arranged by size:
1345 731 702 610 417 391 245 174
54 49 27
EagI C'GGCC_G
Cuts at: 1599 2330 1599
Size: 731 4014
EarI CTCTTCn'nnn_
Cuts at: 684 2768 2978 684
Size: 2084 210 2451
Fragments arranged by size:
2451 2084 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2953 3263 3409 4555 2953
Size: 310 146 1146 3143
Fragments arranged by size:
3143 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1757 1757
Size: 4745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 808 820 1159 1744 2570 3002 3749 4017
Size: 525 12 339 585 826 432 747 268
Cuts at: 4017 283
Size: 1011
Fragments arranged by size:
1011 826 747 585 525 432 339 268
12
EcoNI CCTnn'n_nnAGG
Cuts at: 433 604 1723 433
Size: 171 1119 3455
Fragments arranged by size:
3455 1119 171
EcoO109I rG'GnC_Cy
Cuts at: 173 610 828 881 1176 1261 1274 1394 1506
Size: 437 218 53 295 85 13 120 112
Cuts at: 1506 1566 1884 173
Size: 60 318 3034
Fragments arranged by size:
3034 437 318 295 218 120 112 85
60 53 13
EcoRI G'AATT_C
Cuts at: 1749 1749
Size: 4745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 2764 255
Size: 2509 2236
FspI TGC'GCA
Cuts at: 2526 2526
Size: 4745
HaeII r_GCGC'y
Cuts at: 1417 2179 2427 3449 4428 1417
Size: 762 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 762 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 1297 1329 2587 2617 2619 2649 134
Size: 32 1131 32 1258 30 2 30 2230
Fragments arranged by size:
2230 1258 1131 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1784 1784
Size: 4745
KasI G'GCGC_C
Cuts at: 2175 2423 2175
Size: 248 4497
KpnI G_GTAC'C
Cuts at: 657 1765 657
Size: 1108 3637
MluI A'CGCG_T
Cuts at: 4178 4178
Size: 4745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3416 3600 3416
Size: 184 4561
MscI TGG'CCA
Cuts at: 226 643 1498 1552 2506 226
Size: 417 855 54 954 2465
Fragments arranged by size:
2465 954 855 417 54
MslI CAynn'nnrTG
Cuts at: 118 2734 2861 118
Size: 2616 127 2002
Fragments arranged by size:
2616 2002 127
MspA1I CmG'CkG
Cuts at: 786 876 1157 1236 1856 2170 2530 3543 3788
Size: 90 281 79 620 314 360 1013 245
Cuts at: 3788 786
Size: 1743
Fragments arranged by size:
1743 1013 620 360 314 281 245 90
79
NaeI GCC'GGC
Cuts at: 2926 2926
Size: 4745
NarI GG'CG_CC
Cuts at: 2176 2424 2176
Size: 248 4497
NgoMIV G'CCGG_C
Cuts at: 2924 2924
Size: 4745
NspI r_CATG'y
Cuts at: 711 1798 2298 3205 711
Size: 1087 500 907 2251
Fragments arranged by size:
2251 1087 907 500
PasI CC'CwG_GG
Cuts at: 591 1399 1638 591
Size: 808 239 3698
Fragments arranged by size:
3698 808 239
PciI A'CATG_T
Cuts at: 707 3201 707
Size: 2494 2251
PflMI CCAn_nnn'nTGG
Cuts at: 650 786 650
Size: 136 4609
PfoI T'CCnGG_A
Cuts at: 281 299 539 797 1136 281
Size: 18 240 258 339 3890
Fragments arranged by size:
3890 339 258 240 18
PmeI GTTT'AAAC
Cuts at: 1744 1744
Size: 4745
PmlI CAC'GTG
Cuts at: 447 447
Size: 4745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4713
PpuMI rG'GwC_Cy
Cuts at: 828 881 1261 1566 828
Size: 53 380 305 4007
Fragments arranged by size:
4007 380 305 53
PspOMI G'GGCC_C
Cuts at: 1176 1176
Size: 4745
PstI C_TGCA'G
Cuts at: 437 1007 1792 2477 437
Size: 570 785 685 2705
Fragments arranged by size:
2705 785 685 570
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4745
PvuII CAG'CTG
Cuts at: 786 876 1236 2170 2530 786
Size: 90 360 934 360 3001
Fragments arranged by size:
3001 934 360 360 90
RsrII CG'GwC_CG
Cuts at: 2940 2940
Size: 4745
SacI G_AGCT'C
Cuts at: 1759 1759
Size: 4745
SalI G'TCGA_C
Cuts at: 1782 1782
Size: 4745
SanDI GG'GwC_CC
Cuts at: 881 1261 881
Size: 380 4365
SapI GCTCTTCn'nnn_
Cuts at: 2768 2978 2768
Size: 210 4535
SbfI CC_TGCA'GG
Cuts at: 1792 1792
Size: 4745
ScaI AGT'ACT
Cuts at: 4262 4262
Size: 4745
SexAI A'CCwGG_T
Cuts at: 374 374
Size: 4745
SfcI C'TryA_G
Cuts at: 33 433 1003 1788 2473 3466 3657 33
Size: 400 570 785 685 993 191 1121
Fragments arranged by size:
1121 993 785 685 570 400 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1504 1504
Size: 4745
SfoI GGC'GCC
Cuts at: 2177 2425 2177
Size: 248 4497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
SmaI CCC'GGG
Cuts at: 1075 1767 1075
Size: 692 4053
SmlI C'TyrA_G
Cuts at: 1164 1900 2815 3307 3569 1164
Size: 736 915 492 262 2340
Fragments arranged by size:
2340 915 736 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4745
SphI G_CATG'C
Cuts at: 1798 2298 1798
Size: 500 4245
StuI AGG'CCT
Cuts at: 937 937
Size: 4745
StyI C'CwwG_G
Cuts at: 696 938 1377 1879 2233 696
Size: 242 439 502 354 3208
Fragments arranged by size:
3208 502 439 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2328 2461 2920 3129 2328
Size: 133 459 209 3944
Fragments arranged by size:
3944 459 209 133
TatI w'GTAC_w
Cuts at: 4260 4260
Size: 4745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 436 468 4262 4294 198
Size: 32 206 32 3794 32 649
Fragments arranged by size:
3794 649 206 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2542 2542
Size: 4745
XbaI T'CTAG_A
Cuts at: 1776 1776
Size: 4745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 2240 537
Size: 1703 3042
XhoI C'TCGA_G
Cuts at: 1164 1164
Size: 4745
XmaI C'CCGG_G
Cuts at: 1073 1765 1073
Size: 692 4053
ZraI GAC'GTC
Cuts at: 441 441
Size: 4745
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI BbsI BcgI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI SwaI XmnI