Restriction map for
pF1KE0979
(Circular) (Six-base) MAPSORT of: pF1KE0979.seq Check: 6028 from: 1 to: 4835
pF1KE0979 4835 bp
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 211 1297 211
Size: 1086 3749
AccI GT'mk_AC
Cuts at: 692 1873 692
Size: 1181 3654
Acc65I G'GTAC_C
Cuts at: 1719 1851 1719
Size: 132 4703
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 377 406 776 2660 3092 3839 4107 200
Size: 177 29 370 1884 432 747 268 928
Fragments arranged by size:
1884 928 747 432 370 268 177 29
AfeI AGC'GCT
Cuts at: 958 958
Size: 4835
AflIII A'CryG_T
Cuts at: 1295 2187 3291 4268 1295
Size: 892 1104 977 1862
Fragments arranged by size:
1862 1104 977 892
AgeI A'CCGG_T
Cuts at: 167 3223 167
Size: 3056 1779
AhdI GACnn_n'nnGTC
Cuts at: 1299 1299
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 439 2140 3707 439
Size: 1701 1567 1567
Fragments arranged by size:
1701 1567 1567
ApaLI G'TGCA_C
Cuts at: 3605 3605
Size: 4835
ApoI r'AATT_y
Cuts at: 207 546 742 899 978 1638 1839 4188 4444
Size: 339 196 157 79 660 201 2349 256
Cuts at: 4444 207
Size: 598
Fragments arranged by size:
2349 660 598 339 256 201 196 157
79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 1855 1855
Size: 4835
AvrII C'CTAG_G
Cuts at: 1222 1222
Size: 4835
BamHI G'GATC_C
Cuts at: 1402 1860 1402
Size: 458 4377
BanI G'GyrC_C
Cuts at: 668 1719 1851 2265 2513 2548 668
Size: 1051 132 414 248 35 2955
Fragments arranged by size:
2955 1051 414 248 132 35
BanII G_rGCy'C
Cuts at: 1015 1849 2879 1015
Size: 834 1030 2971
Fragments arranged by size:
2971 1030 834
BbeI G_GCGC'C
Cuts at: 672 2269 2517 672
Size: 1597 248 2990
Fragments arranged by size:
2990 1597 248
BbsI GAAGACnn'nnnn_
Cuts at: 1588 1588
Size: 4835
BciVI GTATCCnnnnn_n'
Cuts at: 2733 3494 4835 2733
Size: 761 1341 2733
Fragments arranged by size:
2733 1341 761
BclI T'GATC_A
Cuts at: 489 2355 489
Size: 1866 2969
BglI GCCn_nnn'nGGC
Cuts at: 112 1225 112
Size: 1113 3722
BglII A'GATC_T
Cuts at: 1758 2350 4387 1758
Size: 592 2037 2206
Fragments arranged by size:
2206 2037 592
BlpI GC'TnA_GC
Cuts at: 761 1947 761
Size: 1186 3649
Bme1580I G_kGCm'C
Cuts at: 1273 2460 2553 3609 1273
Size: 1187 93 1056 2499
Fragments arranged by size:
2499 1187 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 823 2047 2128 2463 4607 823
Size: 1224 81 335 2144 1051
Fragments arranged by size:
2144 1224 1051 335 81
BmtI G_CTAG'C
Cuts at: 1151 1151
Size: 4835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 4835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1604 538
Size: 1066 3769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1500 2011 2890 3382 3680 1500
Size: 511 879 492 298 2655
Fragments arranged by size:
2655 879 511 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4539 4539
Size: 4835
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 1763 2349 4386 6
Size: 1757 586 2037 455
Fragments arranged by size:
2037 1757 586 455
BsaHI Gr'CG_yC
Cuts at: 115 669 1206 2266 2514 3216 4712 115
Size: 554 537 1060 248 702 1496 238
Fragments arranged by size:
1496 1060 702 554 537 248 238
BsaWI w'CCGG_w
Cuts at: 167 2024 2545 3223 3497 3644 4055 167
Size: 1857 521 678 274 147 411 947
Fragments arranged by size:
1857 947 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 511
Size: 30 4805
BseYI C'CCAG_C
Cuts at: 158 439 646 1143 2260 3018 3595 4091 158
Size: 281 207 497 1117 758 577 496 902
Fragments arranged by size:
1117 902 758 577 497 496 281 207
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3631 104
Size: 2273 46 1208 1308
Fragments arranged by size:
2273 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1251 1849 2627 2817 3609 1251
Size: 598 778 190 792 2477
Fragments arranged by size:
2477 792 778 598 190
BsmI GAATG_Cn'
Cuts at: 608 608
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 1015 1251 1273 1849 2460 2553 2627 2817 2879
Size: 236 22 576 611 93 74 190 62
Cuts at: 2879 3609 1015
Size: 730 2241
Fragments arranged by size:
2241 730 611 576 236 190 93 74
62 22
BspEI T'CCGG_A
Cuts at: 4055 4055
Size: 4835
BspHI T'CATG_A
Cuts at: 1028 4011 1028
Size: 2983 1852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 211 1203 1219 1297 1748 1885 2099 2401 2782
Size: 992 16 78 451 137 214 302 381
Cuts at: 2782 211
Size: 2264
Fragments arranged by size:
2264 992 451 381 302 214 137 78
16
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2747 2747
Size: 4835
BsrFI r'CCGG_y
Cuts at: 167 2833 3014 3223 167
Size: 2666 181 209 1779
Fragments arranged by size:
2666 1779 209 181
BssSI C'ACGA_G
Cuts at: 3106 3464 3106
Size: 358 4477
BstAPI GCAn_nnn'nTGC
Cuts at: 2212 2212
Size: 4835
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstYI r'GATC_y
Cuts at: 1 934 1402 1758 1860 2327 2350 2685 2931
Size: 933 468 356 102 467 23 335 246
Cuts at: 2931 3943 4029 4041 4387 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 933 468 467 449 356 346 335
246 102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 836 836
Size: 4835
BtgI C'CryG_G
Cuts at: 107 107
Size: 4835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 2078 1025
Size: 1053 3782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1028 1063 1028
Size: 35 4800
DraI TTT'AAA
Cuts at: 191 213 1647 1815 1834 191
Size: 22 1434 168 19 3192
Fragments arranged by size:
3192 1434 168 22 19
DraIII CAC_nnn'GTG
Cuts at: 823 823
Size: 4835
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3399 2541
Size: 858 3977
EaeI y'GGCC_r
Cuts at: 329 2420 2594 2985 3012 4357 329
Size: 2091 174 391 27 1345 807
Fragments arranged by size:
2091 1345 807 391 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 353 2858 3068 353
Size: 2505 210 2120
Fragments arranged by size:
2505 2120 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3353 3499 4645 3043
Size: 310 146 1146 3233
Fragments arranged by size:
3233 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 377 406 538 776 1604 2660 3092 3839
Size: 177 29 132 238 828 1056 432 747
Cuts at: 3839 4107 200
Size: 268 928
Fragments arranged by size:
1056 928 828 747 432 268 238 177
132 29
EcoNI CCTnn'n_nnAGG
Cuts at: 1226 1226
Size: 4835
EcoO109I rG'GnC_Cy
Cuts at: 352 1974 352
Size: 1622 3213
EcoRI G'AATT_C
Cuts at: 546 1638 1839 546
Size: 1092 201 3542
Fragments arranged by size:
3542 1092 201
EcoRV GAT'ATC
Cuts at: 1342 1342
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 702 2854 702
Size: 2152 2683
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 672 960 2269 2517 3539 4518 672
Size: 288 1309 248 1022 979 989
Fragments arranged by size:
1309 1022 989 979 288 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 511 543 838 870 1042 1074 1285 1317 1400
Size: 32 295 32 172 32 211 32 83
Cuts at: 1400 1432 2677 2707 2709 2739 511
Size: 32 1245 30 2 30 2607
Fragments arranged by size:
2607 1245 295 211 172 83 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1532 1874 1532
Size: 342 4493
HindIII A'AGCT_T
Cuts at: 244 596 866 1595 1730 1793 244
Size: 352 270 729 135 63 3286
Fragments arranged by size:
3286 729 352 270 135 63
HpaI GTT'AAC
Cuts at: 1532 1532
Size: 4835
KasI G'GCGC_C
Cuts at: 668 2265 2513 668
Size: 1597 248 2990
Fragments arranged by size:
2990 1597 248
KpnI G_GTAC'C
Cuts at: 1723 1855 1723
Size: 132 4703
MfeI C'AATT_G
Cuts at: 1289 1289
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 412 3506 3690 412
Size: 3094 184 1557
Fragments arranged by size:
3094 1557 184
MscI TGG'CCA
Cuts at: 331 2596 331
Size: 2265 2570
MslI CAynn'nnrTG
Cuts at: 635 2824 2951 635
Size: 2189 127 2519
Fragments arranged by size:
2519 2189 127
MspA1I CmG'CkG
Cuts at: 415 1946 2260 2620 3633 3878 415
Size: 1531 314 360 1013 245 1372
Fragments arranged by size:
1531 1372 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3016 3016
Size: 4835
NarI GG'CG_CC
Cuts at: 669 2266 2514 669
Size: 1597 248 2990
Fragments arranged by size:
2990 1597 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4835
NgoMIV G'CCGG_C
Cuts at: 3014 3014
Size: 4835
NheI G'CTAG_C
Cuts at: 1147 1147
Size: 4835
NspI r_CATG'y
Cuts at: 1299 1555 1888 2388 3295 1299
Size: 256 333 500 907 2839
Fragments arranged by size:
2839 907 500 333 256
PciI A'CATG_T
Cuts at: 1295 3291 1295
Size: 1996 2839
PflMI CCAn_nnn'nTGG
Cuts at: 823 823
Size: 4835
PfoI T'CCnGG_A
Cuts at: 1620 1620
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 352 352
Size: 4835
PsiI TTA'TAA
Cuts at: 1352 1352
Size: 4835
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 705 737 705
Size: 32 4803
PstI C_TGCA'G
Cuts at: 1020 1308 1882 2567 1020
Size: 288 574 685 3288
Fragments arranged by size:
3288 685 574 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 415 2260 2620 415
Size: 1845 360 2630
Fragments arranged by size:
2630 1845 360
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 2858 3068 2858
Size: 210 4625
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SfcI C'TryA_G
Cuts at: 33 938 1016 1283 1304 1878 2563 3556 3747
Size: 905 78 267 21 574 685 993 191
Cuts at: 3747 33
Size: 1121
Fragments arranged by size:
1121 993 905 685 574 267 191 78
21
SfoI GGC'GCC
Cuts at: 670 2267 2515 670
Size: 1597 248 2990
Fragments arranged by size:
2990 1597 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 1479 1990 2905 3397 3659 1479
Size: 511 915 492 262 2655
Fragments arranged by size:
2655 915 511 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1555 1888 2388 1555
Size: 333 500 4002
Fragments arranged by size:
4002 500 333
SspI AAT'ATT
Cuts at: 1448 1448
Size: 4835
StuI AGG'CCT
Cuts at: 944 944
Size: 4835
StyI C'CwwG_G
Cuts at: 107 686 708 1222 1969 2323 107
Size: 579 22 514 747 354 2619
Fragments arranged by size:
2619 747 579 514 354 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2418 2551 3010 3219 2418
Size: 133 459 209 4034
Fragments arranged by size:
4034 459 209 133
TatI w'GTAC_w
Cuts at: 1053 4350 1053
Size: 3297 1538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 4803
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1060 1755 1866 1060
Size: 695 111 4029
Fragments arranged by size:
4029 695 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2330
Size: 4835
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BmgBI Bpu10I BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI FseI
FspAI NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI