Restriction map for
pF1KE0981
(Circular) (Six-base) MAPSORT of: pF1KE0981.seq Check: 2346 from: 1 to: 4853
pF1KE0981 4853 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 587 587
Size: 4853
AccI GT'mk_AC
Cuts at: 595 1676 1891 595
Size: 1081 215 3557
Fragments arranged by size:
3557 1081 215
Acc65I G'GTAC_C
Cuts at: 1869 1869
Size: 4853
AclI AA'CG_TT
Cuts at: 1202 4693 1202
Size: 3491 1362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 400 873 931 2678 3110 3857 4125 177
Size: 223 473 58 1747 432 747 268 905
Fragments arranged by size:
1747 905 747 473 432 268 223 58
AfeI AGC'GCT
Cuts at: 967 1492 967
Size: 525 4328
AflIII A'CryG_T
Cuts at: 2205 3309 4286 2205
Size: 1104 977 2772
Fragments arranged by size:
2772 1104 977
AgeI A'CCGG_T
Cuts at: 117 3241 117
Size: 3124 1729
AleI CACnn'nnGTG
Cuts at: 243 778 243
Size: 535 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1057 1314 1057
Size: 257 4596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 646
Size: 32 4821
AlwNI CAG_nnn'CTG
Cuts at: 561 909 933 1107 1272 1839 2158 3725 561
Size: 348 24 174 165 567 319 1567 1689
Fragments arranged by size:
1689 1567 567 348 319 174 165 24
ApaI G_GGCC'C
Cuts at: 630 630
Size: 4853
ApaLI G'TGCA_C
Cuts at: 417 748 1015 3623 417
Size: 331 267 2608 1647
Fragments arranged by size:
2608 1647 331 267
ApoI r'AATT_y
Cuts at: 1857 4206 4462 1857
Size: 2349 256 2248
Fragments arranged by size:
2349 2248 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 589 1873 589
Size: 1284 3569
BamHI G'GATC_C
Cuts at: 1878 1878
Size: 4853
BanI G'GyrC_C
Cuts at: 279 772 1005 1591 1657 1681 1869 2283 2531
Size: 493 233 586 66 24 188 414 248
Cuts at: 2531 2566 279
Size: 35 2566
Fragments arranged by size:
2566 586 493 414 248 233 188 66
35 24
BanII G_rGCy'C
Cuts at: 300 630 698 848 1262 1438 1829 1867 2897
Size: 330 68 150 414 176 391 38 1030
Cuts at: 2897 300
Size: 2256
Fragments arranged by size:
2256 1030 414 391 330 176 150 68
38
BbeI G_GCGC'C
Cuts at: 283 1595 1661 2287 2535 283
Size: 1312 66 626 248 2601
Fragments arranged by size:
2601 1312 626 248 66
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3512 4853 2751
Size: 761 1341 2751
Fragments arranged by size:
2751 1341 761
BclI T'GATC_A
Cuts at: 2373 2373
Size: 4853
BglI GCCn_nnn'nGGC
Cuts at: 511 739 1345 1507 1655 1780 511
Size: 228 606 162 148 125 3584
Fragments arranged by size:
3584 606 228 162 148 125
BglII A'GATC_T
Cuts at: 395 2368 4405 395
Size: 1973 2037 843
Fragments arranged by size:
2037 1973 843
BlpI GC'TnA_GC
Cuts at: 1151 1965 1151
Size: 814 4039
Bme1580I G_kGCm'C
Cuts at: 421 630 752 1010 1019 1814 2478 2571 3627
Size: 209 122 258 9 795 664 93 1056
Cuts at: 3627 421
Size: 1647
Fragments arranged by size:
1647 1056 795 664 258 209 122 93
9
BmrI ACTGGGnnnn_n'
Cuts at: 1029 1372 2065 2146 2481 4625 1029
Size: 343 693 81 335 2144 1257
Fragments arranged by size:
2144 1257 693 343 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 4853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 862 886 1294 1662 1768 1861 213
Size: 649 24 408 368 106 93 3205
Fragments arranged by size:
3205 649 408 368 106 93 24
Bpu10I CC'TnA_GC
Cuts at: 1516 1741 1801 1516
Size: 225 60 4568
Fragments arranged by size:
4568 225 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 688 1708 2029 2908 3400 3698 616
Size: 72 1020 321 879 492 298 1771
Fragments arranged by size:
1771 1020 879 492 321 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 380 1744 1837 4557 380
Size: 1364 93 2720 676
Fragments arranged by size:
2720 1364 676 93
BsaAI yAC'GTr
Cuts at: 543 1664 2206 543
Size: 1121 542 3190
Fragments arranged by size:
3190 1121 542
BsaBI GATnn'nnATC
Cuts at: 6 2367 4404 6
Size: 2361 2037 455
Fragments arranged by size:
2361 2037 455
BsaHI Gr'CG_yC
Cuts at: 280 1334 1592 1658 2284 2532 3234 4730 280
Size: 1054 258 66 626 248 702 1496 403
Fragments arranged by size:
1496 1054 702 626 403 258 248 66
BsaWI w'CCGG_w
Cuts at: 117 2042 2563 3241 3515 3662 4073 117
Size: 1925 521 678 274 147 411 897
Fragments arranged by size:
1925 897 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 460 490 460
Size: 30 4823
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 345 1291 1837 280
Size: 65 946 546 3296
Fragments arranged by size:
3296 946 546 65
BseYI C'CCAG_C
Cuts at: 549 877 893 1815 2278 3036 3613 4109 549
Size: 328 16 922 463 758 577 496 1293
Fragments arranged by size:
1293 922 758 577 496 463 328 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 142 748 814 136
Size: 6 606 66 4175
Fragments arranged by size:
4175 606 66 6
BsiEI CG_ry'CG
Cuts at: 104 118 2395 2441 3649 104
Size: 14 2277 46 1208 1308
Fragments arranged by size:
2277 1308 1208 46 14
BsiHKAI G_wGCw'C
Cuts at: 260 421 568 698 752 848 1019 1046 1517
Size: 161 147 130 54 96 171 27 471
Cuts at: 1517 1791 1829 1867 2645 2835 3627 260
Size: 274 38 38 778 190 792 1486
Fragments arranged by size:
1486 792 778 471 274 190 171 161
147 130 96 54 38 38 27
Bsp1286I G_dGCh'C
Cuts at: 260 300 421 568 630 698 752 848 1010
Size: 40 121 147 62 68 54 96 162
Cuts at: 1010 1019 1046 1262 1438 1517 1791 1814 1829
Size: 9 27 216 176 79 274 23 15
Cuts at: 1829 1867 2478 2571 2645 2835 2897 3627 260
Size: 38 611 93 74 190 62 730 1486
Fragments arranged by size:
1486 730 611 274 216 190 176 162
147 121 96 93 79 74 68 62
62 54 40 38 27 23 15 9
BspEI T'CCGG_A
Cuts at: 4073 4073
Size: 4853
BspHI T'CATG_A
Cuts at: 4029 4029
Size: 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 587 815 1903 2117 2419 2800 362
Size: 225 228 1088 214 302 381 2415
Fragments arranged by size:
2415 1088 381 302 228 225 214
BsrBI CCG'CTC
Cuts at: 50 3145 3199 4683 4848 50
Size: 3095 54 1484 165 55
Fragments arranged by size:
3095 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2765 2765
Size: 4853
BsrFI r'CCGG_y
Cuts at: 117 1063 1168 2851 3032 3241 117
Size: 946 105 1683 181 209 1729
Fragments arranged by size:
1729 1683 946 209 181 105
BssHII G'CGCG_C
Cuts at: 1569 1569
Size: 4853
BssSI C'ACGA_G
Cuts at: 343 691 1784 1807 3124 3482 343
Size: 348 1093 23 1317 358 1714
Fragments arranged by size:
1714 1317 1093 358 348 23
BstAPI GCAn_nnn'nTGC
Cuts at: 2230 2230
Size: 4853
BstBI TT'CG_AA
Cuts at: 3214 3214
Size: 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 1542 1542
Size: 4853
BstYI r'GATC_y
Cuts at: 1 395 1878 2345 2368 2703 2949 3961 4047
Size: 394 1483 467 23 335 246 1012 86
Cuts at: 4047 4059 4405 1
Size: 12 346 449
Fragments arranged by size:
1483 1012 467 449 394 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 596 596
Size: 4853
BtgI C'CryG_G
Cuts at: 266 1254 1528 1598 266
Size: 988 274 70 3521
Fragments arranged by size:
3521 988 274 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2803 2984 3147 2803
Size: 181 163 4509
Fragments arranged by size:
4509 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1118 2096 1046
Size: 72 978 3803
Fragments arranged by size:
3803 978 72
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 2559 3417 2559
Size: 858 3995
EaeI y'GGCC_r
Cuts at: 172 1757 2438 2612 3003 3030 4375 172
Size: 1585 681 174 391 27 1345 650
Fragments arranged by size:
1585 1345 681 650 391 174 27
EagI C'GGCC_G
Cuts at: 2438 2438
Size: 4853
EarI CTCTTCn'nnn_
Cuts at: 322 440 703 1451 2876 3086 322
Size: 118 263 748 1425 210 2089
Fragments arranged by size:
2089 1425 748 263 210 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3061 3371 3517 4663 3061
Size: 310 146 1146 3251
Fragments arranged by size:
3251 1146 310 146
EcoICRI GAG'CTC
Cuts at: 696 846 1827 1865 696
Size: 150 981 38 3684
Fragments arranged by size:
3684 981 150 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 213 400 862 873 886 931 1294 1662
Size: 36 187 462 11 13 45 363 368
Cuts at: 1662 1768 1861 2678 3110 3857 4125 177
Size: 106 93 817 432 747 268 905
Fragments arranged by size:
905 817 747 462 432 368 363 268
187 106 93 45 36 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 742 925 742
Size: 183 4670
EcoO109I rG'GnC_Cy
Cuts at: 210 534 626 627 681 897 906 917 1992
Size: 324 92 1 54 216 9 11 1075
Cuts at: 1992 210
Size: 3071
Fragments arranged by size:
3071 1075 324 216 92 54 11 9
1
EcoRI G'AATT_C
Cuts at: 1857 1857
Size: 4853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 657 2872 657
Size: 2215 2638
FspI TGC'GCA
Cuts at: 1552 2634 1552
Size: 1082 3771
HaeII r_GCGC'y
Cuts at: 283 366 969 1410 1494 1595 1661 1741 2287
Size: 83 603 441 84 101 66 80 546
Cuts at: 2287 2535 3557 4536 283
Size: 248 1022 979 600
Fragments arranged by size:
1022 979 603 600 546 441 248 101
84 83 80 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 2695 2725 2727 2757 460
Size: 32 2203 30 2 30 2556
Fragments arranged by size:
2556 2203 32 30 30 2
HincII GTy'rAC
Cuts at: 1077 1892 1077
Size: 815 4038
KasI G'GCGC_C
Cuts at: 279 1591 1657 2283 2531 279
Size: 1312 66 626 248 2601
Fragments arranged by size:
2601 1312 626 248 66
KpnI G_GTAC'C
Cuts at: 1873 1873
Size: 4853
MfeI C'AATT_G
Cuts at: 722 722
Size: 4853
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3524 3708 3524
Size: 184 4669
MscI TGG'CCA
Cuts at: 1759 2614 1759
Size: 855 3998
MslI CAynn'nnrTG
Cuts at: 243 778 1510 2842 2969 243
Size: 535 732 1332 127 2127
Fragments arranged by size:
2127 1332 732 535 127
MspA1I CmG'CkG
Cuts at: 268 492 893 1305 1428 1530 1819 1964 2278
Size: 224 401 412 123 102 289 145 314
Cuts at: 2278 2638 3651 3896 268
Size: 360 1013 245 1225
Fragments arranged by size:
1225 1013 412 401 360 314 289 245
224 145 123 102
NaeI GCC'GGC
Cuts at: 1065 3034 1065
Size: 1969 2884
NarI GG'CG_CC
Cuts at: 280 1592 1658 2284 2532 280
Size: 1312 66 626 248 2601
Fragments arranged by size:
2601 1312 626 248 66
NgoMIV G'CCGG_C
Cuts at: 1063 3032 1063
Size: 1969 2884
NspI r_CATG'y
Cuts at: 1906 2406 3313 1906
Size: 500 907 3446
Fragments arranged by size:
3446 907 500
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4853
PflMI CCAn_nnn'nTGG
Cuts at: 651 840 933 1107 1195 1272 1839 651
Size: 189 93 174 88 77 567 3665
Fragments arranged by size:
3665 567 189 174 93 88 77
PfoI T'CCnGG_A
Cuts at: 644 701 902 1271 644
Size: 57 201 369 4226
Fragments arranged by size:
4226 369 201 57
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4821
PpuMI rG'GwC_Cy
Cuts at: 897 906 917 897
Size: 9 11 4833
Fragments arranged by size:
4833 11 9
PspOMI G'GGCC_C
Cuts at: 626 626
Size: 4853
PstI C_TGCA'G
Cuts at: 395 1900 2585 395
Size: 1505 685 2663
Fragments arranged by size:
2663 1505 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 893 1305 1428 2278 2638 893
Size: 412 123 850 360 3108
Fragments arranged by size:
3108 850 412 360 123
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 698 848 1829 1867 698
Size: 150 981 38 3684
Fragments arranged by size:
3684 981 150 38
SacII CC_GC'GG
Cuts at: 269 1531 269
Size: 1262 3591
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SanDI GG'GwC_CC
Cuts at: 917 917
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 703 2876 3086 703
Size: 2173 210 2470
Fragments arranged by size:
2470 2173 210
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 4370 4370
Size: 4853
SfcI C'TryA_G
Cuts at: 33 391 1896 2581 3574 3765 33
Size: 358 1505 685 993 191 1121
Fragments arranged by size:
1505 1121 993 685 358 191
SfoI GGC'GCC
Cuts at: 281 1593 1659 2285 2533 281
Size: 1312 66 626 248 2601
Fragments arranged by size:
2601 1312 626 248 66
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SmaI CCC'GGG
Cuts at: 1875 1875
Size: 4853
SmlI C'TyrA_G
Cuts at: 631 667 1723 2008 2923 3415 3677 631
Size: 36 1056 285 915 492 262 1807
Fragments arranged by size:
1807 1056 915 492 285 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 1906 2406 1906
Size: 500 4353
StuI AGG'CCT
Cuts at: 1144 1144
Size: 4853
StyI C'CwwG_G
Cuts at: 220 1703 1754 1987 2341 220
Size: 1483 51 233 354 2732
Fragments arranged by size:
2732 1483 354 233 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2436 2569 3028 3237 2436
Size: 133 459 209 4052
Fragments arranged by size:
4052 459 209 133
TatI w'GTAC_w
Cuts at: 797 4368 797
Size: 3571 1282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 4370 4402 397
Size: 32 3941 32 848
Fragments arranged by size:
3941 848 32 32
Tth111I GACn'n_nGTC
Cuts at: 2650 2650
Size: 4853
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 2348 270
Size: 2078 2775
XmaI C'CCGG_G
Cuts at: 1873 1873
Size: 4853
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI