Restriction map for
pF1KE0985
(Circular) (Six-base) MAPSORT of: pF1KE0985.seq Check: 1810 from: 1 to: 4901
pF1KE0985 4901 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 546 546
Size: 4901
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4901
Acc65I G'GTAC_C
Cuts at: 298 1917 298
Size: 1619 3282
AclI AA'CG_TT
Cuts at: 1586 4741 1586
Size: 3155 1746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1444 2726 3158 3905 4173 277
Size: 1167 1282 432 747 268 1005
Fragments arranged by size:
1282 1167 1005 747 432 268
AfeI AGC'GCT
Cuts at: 1839 1839
Size: 4901
AflII C'TTAA_G
Cuts at: 1768 1786 1768
Size: 18 4883
AflIII A'CryG_T
Cuts at: 1499 2253 3357 4334 1499
Size: 754 1104 977 2066
Fragments arranged by size:
2066 1104 977 754
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AleI CACnn'nnGTG
Cuts at: 1258 1258
Size: 4901
AlwNI CAG_nnn'CTG
Cuts at: 2206 3773 2206
Size: 1567 3334
ApaI G_GGCC'C
Cuts at: 365 386 1689 365
Size: 21 1303 3577
Fragments arranged by size:
3577 1303 21
ApaLI G'TGCA_C
Cuts at: 3671 3671
Size: 4901
ApoI r'AATT_y
Cuts at: 140 952 1905 4254 4510 140
Size: 812 953 2349 256 531
Fragments arranged by size:
2349 953 812 531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4901
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 780 1869 1921 780
Size: 1089 52 3760
Fragments arranged by size:
3760 1089 52
BamHI G'GATC_C
Cuts at: 243 471 666 1286 1926 243
Size: 228 195 620 640 3218
Fragments arranged by size:
3218 640 620 228 195
BanI G'GyrC_C
Cuts at: 165 298 642 1917 2331 2579 2614 165
Size: 133 344 1275 414 248 35 2452
Fragments arranged by size:
2452 1275 414 344 248 133 35
BanII G_rGCy'C
Cuts at: 365 386 535 629 1208 1689 1915 2945 365
Size: 21 149 94 579 481 226 1030 2321
Fragments arranged by size:
2321 1030 579 481 226 149 94 21
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbvCI CC'TCA_GC
Cuts at: 928 928
Size: 4901
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 1517 2421 1517
Size: 904 3997
BglI GCCn_nnn'nGGC
Cuts at: 752 752
Size: 4901
BglII A'GATC_T
Cuts at: 1274 1778 2416 4453 1274
Size: 504 638 2037 1722
Fragments arranged by size:
2037 1722 638 504
BlpI GC'TnA_GC
Cuts at: 446 1534 2013 446
Size: 1088 479 3334
Fragments arranged by size:
3334 1088 479
Bme1580I G_kGCm'C
Cuts at: 365 386 647 1689 1878 2526 2619 3675 365
Size: 21 261 1042 189 648 93 1056 1591
Fragments arranged by size:
1591 1056 1042 648 261 189 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 1698 2113 2194 2529 4673 1698
Size: 415 81 335 2144 1926
Fragments arranged by size:
2144 1926 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 4901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 1114 561
Size: 553 4348
Bpu10I CC'TnA_GC
Cuts at: 928 928
Size: 4901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1312 2077 2956 3448 3746 1018
Size: 294 765 879 492 298 2173
Fragments arranged by size:
2173 879 765 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 415 1567 1612 4605 415
Size: 1152 45 2993 711
Fragments arranged by size:
2993 1152 711 45
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 2415 4452 6
Size: 2409 2037 455
Fragments arranged by size:
2409 2037 455
BsaHI Gr'CG_yC
Cuts at: 1764 2332 2580 3282 4778 1764
Size: 568 248 702 1496 1887
Fragments arranged by size:
1887 1496 702 568 248
BsaWI w'CCGG_w
Cuts at: 240 2090 2611 3289 3563 3710 4121 240
Size: 1850 521 678 274 147 411 1020
Fragments arranged by size:
1850 1020 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1468 1498 1642 1672 1468
Size: 30 144 30 4697
Fragments arranged by size:
4697 144 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 871
Size: 4901
BseYI C'CCAG_C
Cuts at: 135 379 401 556 2326 3084 3661 4157 135
Size: 244 22 155 1770 758 577 496 879
Fragments arranged by size:
1770 879 758 577 496 244 155 22
BsiEI CG_ry'CG
Cuts at: 104 2443 2489 3697 104
Size: 2339 46 1208 1308
Fragments arranged by size:
2339 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 629 1208 1915 2693 2883 3675 629
Size: 579 707 778 190 792 1855
Fragments arranged by size:
1855 792 778 707 579 190
Bsp1286I G_dGCh'C
Cuts at: 365 386 535 629 647 1208 1689 1878 1915
Size: 21 149 94 18 561 481 189 37
Cuts at: 1915 2526 2619 2693 2883 2945 3675 365
Size: 611 93 74 190 62 730 1591
Fragments arranged by size:
1591 730 611 561 481 190 189 149
94 93 74 62 37 21 18
BspEI T'CCGG_A
Cuts at: 240 4121 240
Size: 3881 1020
BspHI T'CATG_A
Cuts at: 1106 1799 4077 1106
Size: 693 2278 1930
Fragments arranged by size:
2278 1930 693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 546 1018 1951 2165 2467 2848 546
Size: 472 933 214 302 381 2599
Fragments arranged by size:
2599 933 472 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1538 3193 3247 4731 4896 50
Size: 1488 1655 54 1484 165 55
Fragments arranged by size:
1655 1488 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 2813 1826
Size: 987 3914
BsrFI r'CCGG_y
Cuts at: 2899 3080 3289 2899
Size: 181 209 4511
Fragments arranged by size:
4511 209 181
BssSI C'ACGA_G
Cuts at: 3172 3530 3172
Size: 358 4543
BstAPI GCAn_nnn'nTGC
Cuts at: 2278 2278
Size: 4901
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 475 475
Size: 4901
BstYI r'GATC_y
Cuts at: 1 243 471 666 1274 1286 1778 1926 2393
Size: 242 228 195 608 12 492 148 467
Cuts at: 2393 2416 2751 2997 4009 4095 4107 4453 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 608 492 467 449 346 335 246
242 228 195 148 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 459 1460 459
Size: 1001 3900
BtgI C'CryG_G
Cuts at: 107 365 662 1256 107
Size: 258 297 594 3752
Fragments arranged by size:
3752 594 297 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2851 3032 3195 2851
Size: 181 163 4557
Fragments arranged by size:
4557 181 163
BtsI GCAGTG_nn'
Cuts at: 381 954 2144 381
Size: 573 1190 3138
Fragments arranged by size:
3138 1190 573
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3465 2607
Size: 858 4043
EaeI y'GGCC_r
Cuts at: 596 753 2486 2660 3051 3078 4423 596
Size: 157 1733 174 391 27 1345 1074
Fragments arranged by size:
1733 1345 1074 391 174 157 27
EagI C'GGCC_G
Cuts at: 2486 2486
Size: 4901
EarI CTCTTCn'nnn_
Cuts at: 1213 1297 1610 1798 2924 3134 1213
Size: 84 313 188 1126 210 2980
Fragments arranged by size:
2980 1126 313 210 188 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1214 1753 3109 3419 3565 4711 1214
Size: 539 1356 310 146 1146 1404
Fragments arranged by size:
1404 1356 1146 539 310 146
EcoICRI GAG'CTC
Cuts at: 627 1206 1913 627
Size: 579 707 3615
Fragments arranged by size:
3615 707 579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 561 1114 1444 2726 3158 3905 4173 277
Size: 284 553 330 1282 432 747 268 1005
Fragments arranged by size:
1282 1005 747 553 432 330 284 268
EcoO109I rG'GnC_Cy
Cuts at: 175 331 361 383 834 1439 1685 2040 175
Size: 156 30 22 451 605 246 355 3036
Fragments arranged by size:
3036 605 451 355 246 156 30 22
EcoRI G'AATT_C
Cuts at: 140 952 1905 140
Size: 812 953 3136
Fragments arranged by size:
3136 953 812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 1839 2920 1449
Size: 390 1081 3430
Fragments arranged by size:
3430 1081 390
FspI TGC'GCA
Cuts at: 2682 2682
Size: 4901
HaeII r_GCGC'y
Cuts at: 979 1812 1841 2335 2583 3605 4584 979
Size: 833 29 494 248 1022 979 1296
Fragments arranged by size:
1296 1022 979 833 494 248 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1438 1468 1470 1500 1618 1642 1650 1674 2743
Size: 30 2 30 118 24 8 24 1069
Cuts at: 2743 2773 2775 2805 1438
Size: 30 2 30 3534
Fragments arranged by size:
3534 1069 118 30 30 30 30 24
24 8 2 2
HincII GTy'rAC
Cuts at: 1353 1940 1353
Size: 587 4314
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 302 1921 302
Size: 1619 3282
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1685 3572 3756 1685
Size: 1887 184 2830
Fragments arranged by size:
2830 1887 184
MscI TGG'CCA
Cuts at: 598 755 2662 598
Size: 157 1907 2837
Fragments arranged by size:
2837 1907 157
MslI CAynn'nnrTG
Cuts at: 1258 1525 2890 3017 1258
Size: 267 1365 127 3142
Fragments arranged by size:
3142 1365 267 127
MspA1I CmG'CkG
Cuts at: 367 560 1116 1140 2012 2326 2686 3699 3944
Size: 193 556 24 872 314 360 1013 245
Cuts at: 3944 367
Size: 1324
Fragments arranged by size:
1324 1013 872 556 360 314 245 193
24
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NcoI C'CATG_G
Cuts at: 107 1256 107
Size: 1149 3752
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NspI r_CATG'y
Cuts at: 1503 1530 1954 2454 3361 1503
Size: 27 424 500 907 3043
Fragments arranged by size:
3043 907 500 424 27
PasI CC'CwG_GG
Cuts at: 485 485
Size: 4901
PciI A'CATG_T
Cuts at: 1499 3357 1499
Size: 1858 3043
PflMI CCAn_nnn'nTGG
Cuts at: 876 876
Size: 4901
PfoI T'CCnGG_A
Cuts at: 474 474
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PpuMI rG'GwC_Cy
Cuts at: 175 1439 175
Size: 1264 3637
PshAI GACnn'nnGTC
Cuts at: 427 427
Size: 4901
PspOMI G'GGCC_C
Cuts at: 361 382 1685 361
Size: 21 1303 3577
Fragments arranged by size:
3577 1303 21
PspXI vC'TCGA_Gb
Cuts at: 780 780
Size: 4901
PstI C_TGCA'G
Cuts at: 204 1948 2633 204
Size: 1744 685 2472
Fragments arranged by size:
2472 1744 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 560 1116 1140 2326 2686 560
Size: 556 24 1186 360 2775
Fragments arranged by size:
2775 1186 556 360 24
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 629 1208 1915 629
Size: 579 707 3615
Fragments arranged by size:
3615 707 579
SacII CC_GC'GG
Cuts at: 368 368
Size: 4901
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SanDI GG'GwC_CC
Cuts at: 1439 1439
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 1213 2924 3134 1213
Size: 1711 210 2980
Fragments arranged by size:
2980 1711 210
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 4418 4418
Size: 4901
SfcI C'TryA_G
Cuts at: 33 200 1944 2629 3622 3813 33
Size: 167 1744 685 993 191 1121
Fragments arranged by size:
1744 1121 993 685 191 167
SfiI GGCCn_nnn'nGGCC
Cuts at: 752 752
Size: 4901
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 780 1033 1327 1768 1786 2056 2971 3463 3725
Size: 253 294 441 18 270 915 492 262
Cuts at: 3725 780
Size: 1956
Fragments arranged by size:
1956 915 492 441 294 270 262 253
18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 1954 2454 1954
Size: 500 4401
StuI AGG'CCT
Cuts at: 1031 1031
Size: 4901
StyI C'CwwG_G
Cuts at: 107 327 440 680 968 1046 1256 2035 2389
Size: 220 113 240 288 78 210 779 354
Cuts at: 2389 107
Size: 2619
Fragments arranged by size:
2619 779 354 288 240 220 210 113
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 264 1258 2484 2617 3076 3285 264
Size: 994 1226 133 459 209 1880
Fragments arranged by size:
1880 1226 994 459 209 133
TatI w'GTAC_w
Cuts at: 1187 4416 1187
Size: 3229 1672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 4869
Tth111I GACn'n_nGTC
Cuts at: 1654 2698 1654
Size: 1044 3857
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1365 2396 1365
Size: 1031 3870
XhoI C'TCGA_G
Cuts at: 780 780
Size: 4901
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
XmnI GAAnn'nnTTC
Cuts at: 1822 1822
Size: 4901
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AvrII BaeI BbsI
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PsrI SexAI
SgrAI SnaBI SrfI SspI SwaI ZraI