Restriction map for
pF1KE0988
(Circular) (Six-base) MAPSORT of: pF1KE0988.seq Check: 8500 from: 1 to: 4970
pF1KE0988 4970 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 137 540 137
Size: 403 4567
AccI GT'mk_AC
Cuts at: 2008 2008
Size: 4970
Acc65I G'GTAC_C
Cuts at: 596 1986 596
Size: 1390 3580
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 844 850 877 1123 1240 2795 3227 3974
Size: 645 6 27 246 117 1555 432 747
Cuts at: 3974 4242 199
Size: 268 927
Fragments arranged by size:
1555 927 747 645 432 268 246 117
27 6
AflIII A'CryG_T
Cuts at: 613 2322 3426 4403 613
Size: 1709 1104 977 1180
Fragments arranged by size:
1709 1180 1104 977
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AleI CACnn'nnGTG
Cuts at: 1045 1794 1045
Size: 749 4221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 372 685 1525 1650 372
Size: 313 840 125 3692
Fragments arranged by size:
3692 840 313 125
AlwNI CAG_nnn'CTG
Cuts at: 225 901 2275 3842 225
Size: 676 1374 1567 1353
Fragments arranged by size:
1567 1374 1353 676
ApaI G_GGCC'C
Cuts at: 1928 1928
Size: 4970
ApaLI G'TGCA_C
Cuts at: 259 1480 3740 259
Size: 1221 2260 1489
Fragments arranged by size:
2260 1489 1221
ApoI r'AATT_y
Cuts at: 1974 4323 4579 1974
Size: 2349 256 2365
Fragments arranged by size:
2365 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 430 778 1990 430
Size: 348 1212 3410
Fragments arranged by size:
3410 1212 348
BamHI G'GATC_C
Cuts at: 1995 1995
Size: 4970
BanI G'GyrC_C
Cuts at: 307 596 784 1567 1986 2400 2648 2683 307
Size: 289 188 783 419 414 248 35 2594
Fragments arranged by size:
2594 783 419 414 289 248 188 35
BanII G_rGCy'C
Cuts at: 206 356 845 1060 1082 1928 1984 3014 206
Size: 150 489 215 22 846 56 1030 2162
Fragments arranged by size:
2162 1030 846 489 215 150 56 22
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 441 730 441
Size: 289 4681
BbvCI CC'TCA_GC
Cuts at: 673 1949 673
Size: 1276 3694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4936
BciVI GTATCCnnnnn_n'
Cuts at: 973 2868 3629 4970 973
Size: 1895 761 1341 973
Fragments arranged by size:
1895 1341 973 761
BclI T'GATC_A
Cuts at: 2490 2490
Size: 4970
BfrBI ATG'CAT
Cuts at: 1637 1637
Size: 4970
BglI GCCn_nnn'nGGC
Cuts at: 898 898
Size: 4970
BglII A'GATC_T
Cuts at: 2485 4522 2485
Size: 2037 2933
BlpI GC'TnA_GC
Cuts at: 1596 2082 1596
Size: 486 4484
Bme1580I G_kGCm'C
Cuts at: 263 697 787 1231 1484 1789 1928 2595 2688
Size: 434 90 444 253 305 139 667 93
Cuts at: 2688 3744 263
Size: 1056 1489
Fragments arranged by size:
1489 1056 667 444 434 305 253 139
93 90
BmgBI CAC'GTC
Cuts at: 497 1203 497
Size: 706 4264
BmrI ACTGGGnnnn_n'
Cuts at: 1573 2182 2263 2598 4742 1573
Size: 609 81 335 2144 1801
Fragments arranged by size:
2144 1801 609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 384 3337 384
Size: 2953 2017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 541 1483 1641 1695 526
Size: 15 942 158 54 3801
Fragments arranged by size:
3801 942 158 54 15
Bpu10I CC'TnA_GC
Cuts at: 673 989 1949 673
Size: 316 960 3694
Fragments arranged by size:
3694 960 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1600 2146 3025 3517 3815 1066
Size: 534 546 879 492 298 2221
Fragments arranged by size:
2221 879 546 534 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4674 4674
Size: 4970
BsaAI yAC'GTr
Cuts at: 486 2323 486
Size: 1837 3133
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 467 1316 2401 2649 3351 4847 467
Size: 849 1085 248 702 1496 590
Fragments arranged by size:
1496 1085 849 702 590 248
BsaWI w'CCGG_w
Cuts at: 2159 2680 3358 3632 3779 4190 2159
Size: 521 678 274 147 411 2939
Fragments arranged by size:
2939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1690 1720 1690
Size: 30 4940
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 634 1090 1108 1414 1480 184
Size: 450 456 18 306 66 3674
Fragments arranged by size:
3674 456 450 306 66 18
BseYI C'CCAG_C
Cuts at: 234 918 1357 2395 3153 3730 4226 234
Size: 684 439 1038 758 577 496 978
Fragments arranged by size:
1038 978 758 684 577 496 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 1417 1357
Size: 60 4910
BsiEI CG_ry'CG
Cuts at: 104 421 1027 2512 2558 3766 104
Size: 317 606 1485 46 1208 1308
Fragments arranged by size:
1485 1308 1208 606 317 46
BsiHKAI G_wGCw'C
Cuts at: 176 263 356 1082 1484 1984 2762 2952 3744
Size: 87 93 726 402 500 778 190 792
Cuts at: 3744 176
Size: 1402
Fragments arranged by size:
1402 792 778 726 500 402 190 93
87
BsmI GAATG_Cn'
Cuts at: 773 1639 773
Size: 866 4104
BsmBI CGTCTCn'nnnn_
Cuts at: 458 945 458
Size: 487 4483
Bsp1286I G_dGCh'C
Cuts at: 176 206 263 356 697 787 845 1060 1082
Size: 30 57 93 341 90 58 215 22
Cuts at: 1082 1231 1484 1789 1928 1984 2595 2688 2762
Size: 149 253 305 139 56 611 93 74
Cuts at: 2762 2952 3014 3744 176
Size: 190 62 730 1402
Fragments arranged by size:
1402 730 611 341 305 253 215 190
149 139 93 93 90 74 62 58
57 56 30 22
BspEI T'CCGG_A
Cuts at: 4190 4190
Size: 4970
BspHI T'CATG_A
Cuts at: 1820 4146 1820
Size: 2326 2644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 407 540 2020 2234 2536 2917 137
Size: 270 133 1480 214 302 381 2190
Fragments arranged by size:
2190 1480 381 302 270 214 133
BsrBI CCG'CTC
Cuts at: 50 585 1595 1614 3262 3316 4800 4965 50
Size: 535 1010 19 1648 54 1484 165 55
Fragments arranged by size:
1648 1484 1010 535 165 55 54 19
BsrDI GCAATG_nn'
Cuts at: 317 563 1422 2882 317
Size: 246 859 1460 2405
Fragments arranged by size:
2405 1460 859 246
BsrFI r'CCGG_y
Cuts at: 322 345 416 1520 2968 3149 3358 322
Size: 23 71 1104 1448 181 209 1934
Fragments arranged by size:
1934 1448 1104 209 181 71 23
BssSI C'ACGA_G
Cuts at: 256 580 1041 3241 3599 256
Size: 324 461 2200 358 1627
Fragments arranged by size:
2200 1627 461 358 324
BstAPI GCAn_nnn'nTGC
Cuts at: 267 410 770 1275 1567 1884 2347 267
Size: 143 360 505 292 317 463 2890
Fragments arranged by size:
2890 505 463 360 317 292 143
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstEII G'GTnAC_C
Cuts at: 1686 1686
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 1563 1917 1563
Size: 354 4616
BstYI r'GATC_y
Cuts at: 1 1403 1835 1995 2462 2485 2820 3066 4078
Size: 1402 432 160 467 23 335 246 1012
Cuts at: 4078 4164 4176 4522 1
Size: 86 12 346 449
Fragments arranged by size:
1402 1012 467 449 432 346 335 246
160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 894 894
Size: 4970
BtgI C'CryG_G
Cuts at: 119 470 679 1021 1034 1445 1871 119
Size: 351 209 342 13 411 426 3218
Fragments arranged by size:
3218 426 411 351 342 209 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1682 2920 3101 3264 1682
Size: 1238 181 163 3388
Fragments arranged by size:
3388 1238 181 163
BtsI GCAGTG_nn'
Cuts at: 885 1586 2213 885
Size: 701 627 3642
Fragments arranged by size:
3642 701 627
DraI TTT'AAA
Cuts at: 1969 1969
Size: 4970
DraIII CAC_nnn'GTG
Cuts at: 267 1440 1567 1694 267
Size: 1173 127 127 3543
Fragments arranged by size:
3543 1173 127 127
DrdI GACnn_nn'nnGTC
Cuts at: 2676 3534 2676
Size: 858 4112
EaeI y'GGCC_r
Cuts at: 418 1024 1205 1421 1609 1713 1907 2555 2729
Size: 606 181 216 188 104 194 648 174
Cuts at: 2729 3120 3147 4492 418
Size: 391 27 1345 896
Fragments arranged by size:
1345 896 648 606 391 216 194 188
181 174 104 27
EagI C'GGCC_G
Cuts at: 418 1024 2555 418
Size: 606 1531 2833
Fragments arranged by size:
2833 1531 606
EarI CTCTTCn'nnn_
Cuts at: 211 1102 1244 2993 3203 211
Size: 891 142 1749 210 1978
Fragments arranged by size:
1978 1749 891 210 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 850 3178 3488 3634 4780 211
Size: 639 2328 310 146 1146 401
Fragments arranged by size:
2328 1146 639 401 310 146
EcoICRI GAG'CTC
Cuts at: 354 1080 1982 354
Size: 726 902 3342
Fragments arranged by size:
3342 902 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 526 541 844 850 877 1123 1240 1483
Size: 327 15 303 6 27 246 117 243
Cuts at: 1483 1641 1695 2795 3227 3974 4242 199
Size: 158 54 1100 432 747 268 927
Fragments arranged by size:
1100 927 747 432 327 303 268 246
243 158 117 54 27 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 523 859 523
Size: 336 4634
EcoO109I rG'GnC_Cy
Cuts at: 516 543 1332 1924 1925 2109 516
Size: 27 789 592 1 184 3377
Fragments arranged by size:
3377 789 592 184 27 1
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1113 2989 1113
Size: 1876 3094
FspI TGC'GCA
Cuts at: 403 1186 2751 403
Size: 783 1565 2622
Fragments arranged by size:
2622 1565 783
HaeII r_GCGC'y
Cuts at: 297 2404 2652 3674 4653 297
Size: 2107 248 1022 979 614
Fragments arranged by size:
2107 1022 979 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 930 962 1690 1722 2812 2842 2844 2874 930
Size: 32 728 32 1090 30 2 30 3026
Fragments arranged by size:
3026 1090 728 32 32 30 30 2
HincII GTy'rAC
Cuts at: 938 1518 2009 938
Size: 580 491 3899
Fragments arranged by size:
3899 580 491
HpaI GTT'AAC
Cuts at: 1518 1518
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 600 1990 600
Size: 1390 3580
MluI A'CGCG_T
Cuts at: 613 4403 613
Size: 3790 1180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3641 3825 3641
Size: 184 4786
MscI TGG'CCA
Cuts at: 1207 1909 2731 1207
Size: 702 822 3446
Fragments arranged by size:
3446 822 702
MslI CAynn'nnrTG
Cuts at: 1045 1213 1351 1450 1632 1794 2959 3086 1045
Size: 168 138 99 182 162 1165 127 2929
Fragments arranged by size:
2929 1165 182 168 162 138 127 99
MspA1I CmG'CkG
Cuts at: 121 681 810 1023 1284 1371 2081 2395 2755
Size: 560 129 213 261 87 710 314 360
Cuts at: 2755 3768 4013 121
Size: 1013 245 1078
Fragments arranged by size:
1078 1013 710 560 360 314 261 245
213 129 87
NaeI GCC'GGC
Cuts at: 324 3151 324
Size: 2827 2143
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 470 1034 1445 470
Size: 564 411 3995
Fragments arranged by size:
3995 564 411
NgoMIV G'CCGG_C
Cuts at: 322 3149 322
Size: 2827 2143
NsiI A_TGCA'T
Cuts at: 1639 1639
Size: 4970
NspI r_CATG'y
Cuts at: 1185 1887 2023 2523 3430 1185
Size: 702 136 500 907 2725
Fragments arranged by size:
2725 907 702 500 136
PciI A'CATG_T
Cuts at: 3426 3426
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PspOMI G'GGCC_C
Cuts at: 1924 1924
Size: 4970
PspXI vC'TCGA_Gb
Cuts at: 430 430
Size: 4970
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 810 1284 2395 2755 810
Size: 474 1111 360 3025
Fragments arranged by size:
3025 1111 474 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 356 1082 1984 356
Size: 726 902 3342
Fragments arranged by size:
3342 902 726
SacII CC_GC'GG
Cuts at: 122 682 1024 122
Size: 560 342 4068
Fragments arranged by size:
4068 560 342
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 211 1102 2993 3203 211
Size: 891 1891 210 1978
Fragments arranged by size:
1978 1891 891 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 769 4487 769
Size: 3718 1252
SexAI A'CCwGG_T
Cuts at: 1436 1436
Size: 4970
SfcI C'TryA_G
Cuts at: 33 1062 1899 2013 2698 3691 3882 33
Size: 1029 837 114 685 993 191 1121
Fragments arranged by size:
1121 1029 993 837 685 191 114
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 430 1081 1615 2125 3040 3532 3794 430
Size: 651 534 510 915 492 262 1606
Fragments arranged by size:
1606 915 651 534 510 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 1185 1887 2023 2523 1185
Size: 702 136 500 3632
Fragments arranged by size:
3632 702 500 136
StuI AGG'CCT
Cuts at: 865 1702 865
Size: 837 4133
StyI C'CwwG_G
Cuts at: 470 1034 1445 2104 2458 470
Size: 564 411 659 354 2982
Fragments arranged by size:
2982 659 564 411 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 670 941 1555 2553 2686 3145 3354 670
Size: 271 614 998 133 459 209 2286
Fragments arranged by size:
2286 998 614 459 271 209 133
TatI w'GTAC_w
Cuts at: 767 4485 767
Size: 3718 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1426 1458 4487 4519 1426
Size: 32 3029 32 1877
Fragments arranged by size:
3029 1877 32 32
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 2465
Size: 4970
XhoI C'TCGA_G
Cuts at: 430 430
Size: 4970
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 1096 1096
Size: 4970
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI