Restriction map for
pF1KE0989
(Circular) (Six-base) MAPSORT of: pF1KE0989.seq Check: 1167 from: 1 to: 4982
pF1KE0989 4982 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 4822 4822
Size: 4982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 614 632 1805 2807 3239 3986 4254 289
Size: 325 18 1173 1002 432 747 268 1017
Fragments arranged by size:
1173 1017 1002 747 432 325 268 18
AflIII A'CryG_T
Cuts at: 2334 3438 4415 2334
Size: 1104 977 2901
Fragments arranged by size:
2901 1104 977
AgeI A'CCGG_T
Cuts at: 3370 3370
Size: 4982
AlwNI CAG_nnn'CTG
Cuts at: 1137 1164 2287 3854 1137
Size: 27 1123 1567 2265
Fragments arranged by size:
2265 1567 1123 27
ApaLI G'TGCA_C
Cuts at: 1913 1933 3752 1913
Size: 20 1819 3143
Fragments arranged by size:
3143 1819 20
ApoI r'AATT_y
Cuts at: 740 1986 4335 4591 740
Size: 1246 2349 256 1131
Fragments arranged by size:
2349 1246 1131 256
AseI AT'TA_AT
Cuts at: 20 373 20
Size: 353 4629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 1224 2002 1224
Size: 778 4204
BamHI G'GATC_C
Cuts at: 909 1790 2007 909
Size: 881 217 3884
Fragments arranged by size:
3884 881 217
BanI G'GyrC_C
Cuts at: 1998 2412 2660 2695 1998
Size: 414 248 35 4285
Fragments arranged by size:
4285 414 248 35
BanII G_rGCy'C
Cuts at: 1996 3026 1996
Size: 1030 3952
BbeI G_GCGC'C
Cuts at: 2416 2664 2416
Size: 248 4734
BciVI GTATCCnnnnn_n'
Cuts at: 781 2880 3641 4982 781
Size: 2099 761 1341 781
Fragments arranged by size:
2099 1341 781 761
BclI T'GATC_A
Cuts at: 2502 2502
Size: 4982
BfrBI ATG'CAT
Cuts at: 242 242
Size: 4982
BglII A'GATC_T
Cuts at: 625 2497 4534 625
Size: 1872 2037 1073
Fragments arranged by size:
2037 1872 1073
BlpI GC'TnA_GC
Cuts at: 2094 2094
Size: 4982
Bme1580I G_kGCm'C
Cuts at: 609 1917 1937 2607 2700 3756 609
Size: 1308 20 670 93 1056 1835
Fragments arranged by size:
1835 1308 1056 670 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 819 1669 1959 2194 2275 2610 4754 819
Size: 850 290 235 81 335 2144 1047
Fragments arranged by size:
2144 1047 850 335 290 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1337
Size: 4982
Bpu10I CC'TnA_GC
Cuts at: 1070 1165 1070
Size: 95 4887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 2158 3037 3529 3827 270
Size: 1888 879 492 298 1425
Fragments arranged by size:
1888 1425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4686 4686
Size: 4982
BsaAI yAC'GTr
Cuts at: 149 2335 149
Size: 2186 2796
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 2413 2661 3363 4859 2413
Size: 248 702 1496 2536
Fragments arranged by size:
2536 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2171 2692 3370 3644 3791 4202 2171
Size: 521 678 274 147 411 2951
Fragments arranged by size:
2951 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1112 1325 2407 3165 3742 4238 1112
Size: 213 1082 758 577 496 1856
Fragments arranged by size:
1856 1082 758 577 496 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 1792
Size: 4982
BsiEI CG_ry'CG
Cuts at: 104 925 2524 2570 3778 104
Size: 821 1599 46 1208 1308
Fragments arranged by size:
1599 1308 1208 821 46
BsiHKAI G_wGCw'C
Cuts at: 1917 1937 1996 2774 2964 3756 1917
Size: 20 59 778 190 792 3143
Fragments arranged by size:
3143 792 778 190 59 20
BsmI GAATG_Cn'
Cuts at: 244 244
Size: 4982
Bsp1286I G_dGCh'C
Cuts at: 609 1917 1937 1996 2607 2700 2774 2964 3026
Size: 1308 20 59 611 93 74 190 62
Cuts at: 3026 3756 609
Size: 730 1835
Fragments arranged by size:
1835 1308 730 611 190 93 74 62
59 20
BspEI T'CCGG_A
Cuts at: 4202 4202
Size: 4982
BspHI T'CATG_A
Cuts at: 887 4158 887
Size: 3271 1711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1491 2032 2246 2548 2929 1491
Size: 541 214 302 381 3544
Fragments arranged by size:
3544 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3274 3328 4812 4977 50
Size: 3224 54 1484 165 55
Fragments arranged by size:
3224 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1704 2894 1704
Size: 1190 3792
BsrFI r'CCGG_y
Cuts at: 2980 3161 3370 2980
Size: 181 209 4592
Fragments arranged by size:
4592 209 181
BssSI C'ACGA_G
Cuts at: 3253 3611 3253
Size: 358 4624
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1681 2359 1137
Size: 544 678 3760
Fragments arranged by size:
3760 678 544
BstBI TT'CG_AA
Cuts at: 3343 3343
Size: 4982
BstXI CCAn_nnnn'nTGG
Cuts at: 463 1659 463
Size: 1196 3786
BstYI r'GATC_y
Cuts at: 1 625 909 1790 2007 2474 2497 2832 3078
Size: 624 284 881 217 467 23 335 246
Cuts at: 3078 4090 4176 4188 4534 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 881 624 467 449 346 335 284
246 217 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1443 1443
Size: 4982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4638
Fragments arranged by size:
4638 181 163
BtsI GCAGTG_nn'
Cuts at: 1135 2225 1135
Size: 1090 3892
ClaI AT'CG_AT
Cuts at: 1091 1091
Size: 4982
DraI TTT'AAA
Cuts at: 738 1016 1392 1981 738
Size: 278 376 589 3739
Fragments arranged by size:
3739 589 376 278
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3546 2688
Size: 858 4124
EaeI y'GGCC_r
Cuts at: 197 778 1782 1955 2567 2741 3132 3159 4504
Size: 581 1004 173 612 174 391 27 1345
Cuts at: 4504 197
Size: 675
Fragments arranged by size:
1345 1004 675 612 581 391 174 173
27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 208 1325 3005 3215 208
Size: 1117 1680 210 1975
Fragments arranged by size:
1975 1680 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3190 3500 3646 4792 3190
Size: 310 146 1146 3380
Fragments arranged by size:
3380 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1994 1994
Size: 4982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 614 632 1337 1805 2807 3239 3986 4254
Size: 325 18 705 468 1002 432 747 268
Cuts at: 4254 289
Size: 1017
Fragments arranged by size:
1017 1002 747 705 468 432 325 268
18
EcoO109I rG'GnC_Cy
Cuts at: 979 1663 2121 979
Size: 684 458 3840
Fragments arranged by size:
3840 684 458
EcoRI G'AATT_C
Cuts at: 1986 1986
Size: 4982
EcoRV GAT'ATC
Cuts at: 1564 1564
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1611 3001 1611
Size: 1390 3592
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4982
HaeII r_GCGC'y
Cuts at: 2416 2664 3686 4665 2416
Size: 248 1022 979 2733
Fragments arranged by size:
2733 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 2824 2854 2856 2886 571
Size: 32 2221 30 2 30 2667
Fragments arranged by size:
2667 2221 32 30 30 2
HincII GTy'rAC
Cuts at: 1520 2021 1520
Size: 501 4481
KasI G'GCGC_C
Cuts at: 2412 2660 2412
Size: 248 4734
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 4415 4415
Size: 4982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 645 1083 1316 1475 1552 3653 3837 645
Size: 438 233 159 77 2101 184 1790
Fragments arranged by size:
2101 1790 438 233 184 159 77
MscI TGG'CCA
Cuts at: 199 780 1784 1957 2743 199
Size: 581 1004 173 786 2438
Fragments arranged by size:
2438 1004 786 581 173
MslI CAynn'nnrTG
Cuts at: 124 287 461 652 1277 2971 3098 124
Size: 163 174 191 625 1694 127 2008
Fragments arranged by size:
2008 1694 625 191 174 163 127
MspA1I CmG'CkG
Cuts at: 795 1026 1780 2093 2407 2767 3780 4025 795
Size: 231 754 313 314 360 1013 245 1752
Fragments arranged by size:
1752 1013 754 360 314 313 245 231
NaeI GCC'GGC
Cuts at: 3163 3163
Size: 4982
NarI GG'CG_CC
Cuts at: 2413 2661 2413
Size: 248 4734
NgoMIV G'CCGG_C
Cuts at: 3161 3161
Size: 4982
NsiI A_TGCA'T
Cuts at: 244 244
Size: 4982
NspI r_CATG'y
Cuts at: 1245 2035 2535 3442 1245
Size: 790 500 907 2785
Fragments arranged by size:
2785 907 790 500
PasI CC'CwG_GG
Cuts at: 709 709
Size: 4982
PciI A'CATG_T
Cuts at: 3438 3438
Size: 4982
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4950
PpuMI rG'GwC_Cy
Cuts at: 1663 1663
Size: 4982
PsiI TTA'TAA
Cuts at: 1877 1877
Size: 4982
PstI C_TGCA'G
Cuts at: 1142 2029 2714 1142
Size: 887 685 3410
Fragments arranged by size:
3410 887 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 795 1026 1780 2407 2767 795
Size: 231 754 627 360 3010
Fragments arranged by size:
3010 754 627 360 231
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 1996 1996
Size: 4982
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SanDI GG'GwC_CC
Cuts at: 1663 1663
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 3005 3215 3005
Size: 210 4772
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 1543 4499 1543
Size: 2956 2026
SfcI C'TryA_G
Cuts at: 33 1138 1883 2025 2710 3703 3894 33
Size: 1105 745 142 685 993 191 1121
Fragments arranged by size:
1121 1105 993 745 685 191 142
SfoI GGC'GCC
Cuts at: 2414 2662 2414
Size: 248 4734
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 2004 2004
Size: 4982
SmlI C'TyrA_G
Cuts at: 249 2137 3052 3544 3806 249
Size: 1888 915 492 262 1425
Fragments arranged by size:
1888 1425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 2035 2535 2035
Size: 500 4482
SspI AAT'ATT
Cuts at: 420 434 1902 420
Size: 14 1468 3500
Fragments arranged by size:
3500 1468 14
StyI C'CwwG_G
Cuts at: 2116 2470 2116
Size: 354 4628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 907 939 965 1372 1941 2565 2698 3157 3366
Size: 32 26 407 569 624 133 459 209
Cuts at: 3366 907
Size: 2523
Fragments arranged by size:
2523 624 569 459 407 209 133 32
26
TatI w'GTAC_w
Cuts at: 1541 4497 1541
Size: 2956 2026
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 4950
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 2477 132
Size: 2345 2637
XmaI C'CCGG_G
Cuts at: 2002 2002
Size: 4982
XmnI GAAnn'nnTTC
Cuts at: 878 878
Size: 4982
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I BtgI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI