Restriction map for
pF1KE0990
(Circular) (Six-base) MAPSORT of: pF1KE0990.seq Check: 4268 from: 1 to: 4988
pF1KE0990 4988 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1235 1235
Size: 4988
AatII G_ACGT'C
Cuts at: 779 1240 779
Size: 461 4527
AccI GT'mk_AC
Cuts at: 779 2026 779
Size: 1247 3741
Acc65I G'GTAC_C
Cuts at: 2004 2004
Size: 4988
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 624 1287 1399 2813 3245 3992 4260 599
Size: 25 663 112 1414 432 747 268 1327
Fragments arranged by size:
1414 1327 747 663 432 268 112 25
AflIII A'CryG_T
Cuts at: 2340 3444 4421 2340
Size: 1104 977 2907
Fragments arranged by size:
2907 1104 977
AgeI A'CCGG_T
Cuts at: 3376 3376
Size: 4988
AlwNI CAG_nnn'CTG
Cuts at: 463 1052 1759 2293 3860 463
Size: 589 707 534 1567 1591
Fragments arranged by size:
1591 1567 707 589 534
ApaLI G'TGCA_C
Cuts at: 3758 3758
Size: 4988
ApoI r'AATT_y
Cuts at: 1400 1992 4341 4597 1400
Size: 592 2349 256 1791
Fragments arranged by size:
2349 1791 592 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4988
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 2008 2008
Size: 4988
BamHI G'GATC_C
Cuts at: 2013 2013
Size: 4988
BanI G'GyrC_C
Cuts at: 1743 2004 2418 2666 2701 1743
Size: 261 414 248 35 4030
Fragments arranged by size:
4030 414 261 248 35
BanII G_rGCy'C
Cuts at: 356 552 1123 1973 2002 3032 356
Size: 196 571 850 29 1030 2312
Fragments arranged by size:
2312 1030 850 571 196 29
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BbsI GAAGACnn'nnnn_
Cuts at: 804 1225 1441 804
Size: 421 216 4351
Fragments arranged by size:
4351 421 216
BbvCI CC'TCA_GC
Cuts at: 476 476
Size: 4988
BciVI GTATCCnnnnn_n'
Cuts at: 2886 3647 4988 2886
Size: 761 1341 2886
Fragments arranged by size:
2886 1341 761
BclI T'GATC_A
Cuts at: 212 2508 212
Size: 2296 2692
BglII A'GATC_T
Cuts at: 1466 2503 4540 1466
Size: 1037 2037 1914
Fragments arranged by size:
2037 1914 1037
BlpI GC'TnA_GC
Cuts at: 175 2100 175
Size: 1925 3063
Bme1580I G_kGCm'C
Cuts at: 791 2613 2706 3762 791
Size: 1822 93 1056 2017
Fragments arranged by size:
2017 1822 1056 93
BmgBI CAC'GTC
Cuts at: 714 714
Size: 4988
BmrI ACTGGGnnnn_n'
Cuts at: 2200 2281 2616 4760 2200
Size: 81 335 2144 2428
Fragments arranged by size:
2428 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 219 1845 3355 219
Size: 1626 1510 1852
Fragments arranged by size:
1852 1626 1510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 524 553 704 720 1114 1413 1504 1876
Size: 154 29 151 16 394 299 91 372
Cuts at: 1876 370
Size: 3482
Fragments arranged by size:
3482 394 372 299 154 151 91 29
16
Bpu10I CC'TnA_GC
Cuts at: 476 656 1056 476
Size: 180 400 4408
Fragments arranged by size:
4408 400 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 2164 3043 3535 3833 621
Size: 1543 879 492 298 1776
Fragments arranged by size:
1776 1543 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4692 4692
Size: 4988
BsaAI yAC'GTr
Cuts at: 210 2341 210
Size: 2131 2857
BsaBI GATnn'nnATC
Cuts at: 6 2502 4539 6
Size: 2496 2037 455
Fragments arranged by size:
2496 2037 455
BsaHI Gr'CG_yC
Cuts at: 776 1237 2419 2667 3369 4865 776
Size: 461 1182 248 702 1496 899
Fragments arranged by size:
1496 1182 899 702 461 248
BsaWI w'CCGG_w
Cuts at: 1232 1506 2177 2698 3376 3650 3797 4208 1232
Size: 274 671 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 671 521 411 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 274
Size: 30 4958
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1381 1716 1719 1821 1824 1381
Size: 335 3 102 3 4545
Fragments arranged by size:
4545 335 102 3 3
BseYI C'CCAG_C
Cuts at: 227 924 1037 2413 3171 3748 4244 227
Size: 697 113 1376 758 577 496 971
Fragments arranged by size:
1376 971 758 697 577 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 280
Size: 4988
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3784 104
Size: 2426 46 1208 1308
Fragments arranged by size:
2426 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 356 1123 1973 2002 2780 2970 3762 356
Size: 767 850 29 778 190 792 1582
Fragments arranged by size:
1582 850 792 778 767 190 29
BsmBI CGTCTCn'nnnn_
Cuts at: 1621 1621
Size: 4988
Bsp1286I G_dGCh'C
Cuts at: 356 552 791 1123 1973 2002 2613 2706 2780
Size: 196 239 332 850 29 611 93 74
Cuts at: 2780 2970 3032 3762 356
Size: 190 62 730 1582
Fragments arranged by size:
1582 850 730 611 332 239 196 190
93 74 62 29
BspEI T'CCGG_A
Cuts at: 1232 1506 4208 1232
Size: 274 2702 2012
Fragments arranged by size:
2702 2012 274
BspHI T'CATG_A
Cuts at: 4164 4164
Size: 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1091 1235 2038 2252 2554 2935 1091
Size: 144 803 214 302 381 3144
Fragments arranged by size:
3144 803 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 255 1611 3280 3334 4818 4983 50
Size: 205 1356 1669 54 1484 165 55
Fragments arranged by size:
1669 1484 1356 205 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1355 2900 1355
Size: 1545 3443
BsrFI r'CCGG_y
Cuts at: 390 558 2986 3167 3376 390
Size: 168 2428 181 209 2002
Fragments arranged by size:
2428 2002 209 181 168
BssSI C'ACGA_G
Cuts at: 3259 3617 3259
Size: 358 4630
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 2365 1759
Size: 606 4382
BstBI TT'CG_AA
Cuts at: 3349 3349
Size: 4988
BstXI CCAn_nnnn'nTGG
Cuts at: 1740 1740
Size: 4988
BstYI r'GATC_y
Cuts at: 1 1466 2013 2480 2503 2838 3084 4096 4182
Size: 1465 547 467 23 335 246 1012 86
Cuts at: 4182 4194 4540 1
Size: 12 346 449
Fragments arranged by size:
1465 1012 547 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 248 1532 107
Size: 141 1284 3563
Fragments arranged by size:
3563 1284 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 2231 2231
Size: 4988
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1274 1309 1274
Size: 35 4953
DraI TTT'AAA
Cuts at: 1987 1987
Size: 4988
DrdI GACnn_nn'nnGTC
Cuts at: 1004 2694 3552 1004
Size: 1690 858 2440
Fragments arranged by size:
2440 1690 858
EaeI y'GGCC_r
Cuts at: 512 1322 1529 2573 2747 3138 3165 4510 512
Size: 810 207 1044 174 391 27 1345 990
Fragments arranged by size:
1345 1044 990 810 391 207 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 485 1601 1674 3011 3221 485
Size: 1116 73 1337 210 2252
Fragments arranged by size:
2252 1337 1116 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3196 3506 3652 4798 3196
Size: 310 146 1146 3386
Fragments arranged by size:
3386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 354 1121 1971 2000 354
Size: 767 850 29 3342
Fragments arranged by size:
3342 850 767 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 524 553 599 624 704 720 1114 1287
Size: 154 29 46 25 80 16 394 173
Cuts at: 1287 1399 1413 1504 1876 2813 3245 3992 4260
Size: 112 14 91 372 937 432 747 268
Cuts at: 4260 370
Size: 1098
Fragments arranged by size:
1098 937 747 432 394 372 268 173
154 112 91 80 46 29 25 16
14
EcoNI CCTnn'n_nnAGG
Cuts at: 1486 1576 1486
Size: 90 4898
EcoO109I rG'GnC_Cy
Cuts at: 533 764 1329 1524 2127 533
Size: 231 565 195 603 3394
Fragments arranged by size:
3394 603 565 231 195
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4988
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4988
HaeII r_GCGC'y
Cuts at: 2422 2670 3692 4671 2422
Size: 248 1022 979 2739
Fragments arranged by size:
2739 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1206 1238 1283 1315 1711 1743 1819 1851 2830
Size: 32 45 32 396 32 76 32 979
Cuts at: 2830 2860 2862 2892 1206
Size: 30 2 30 3302
Fragments arranged by size:
3302 979 396 76 45 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1191 1704 2027 1191
Size: 513 323 4152
Fragments arranged by size:
4152 513 323
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 2008 2008
Size: 4988
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1700 3659 3843 1700
Size: 1959 184 2845
Fragments arranged by size:
2845 1959 184
MscI TGG'CCA
Cuts at: 514 1531 2749 514
Size: 1017 1218 2753
Fragments arranged by size:
2753 1218 1017
MslI CAynn'nnrTG
Cuts at: 908 1213 1270 1738 2977 3104 908
Size: 305 57 468 1239 127 2792
Fragments arranged by size:
2792 1239 468 305 127 57
MspA1I CmG'CkG
Cuts at: 129 231 1281 1307 2099 2413 2773 3786 4031
Size: 102 1050 26 792 314 360 1013 245
Cuts at: 4031 129
Size: 1086
Fragments arranged by size:
1086 1050 1013 792 360 314 245 102
26
NaeI GCC'GGC
Cuts at: 560 3169 560
Size: 2609 2379
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NcoI C'CATG_G
Cuts at: 107 248 107
Size: 141 4847
NgoMIV G'CCGG_C
Cuts at: 558 3167 558
Size: 2609 2379
NspI r_CATG'y
Cuts at: 306 2041 2541 3448 306
Size: 1735 500 907 1846
Fragments arranged by size:
1846 1735 907 500
PasI CC'CwG_GG
Cuts at: 529 1748 529
Size: 1219 3769
PciI A'CATG_T
Cuts at: 3444 3444
Size: 4988
PfoI T'CCnGG_A
Cuts at: 846 1429 846
Size: 583 4405
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PpuMI rG'GwC_Cy
Cuts at: 533 764 1329 533
Size: 231 565 4192
Fragments arranged by size:
4192 565 231
PstI C_TGCA'G
Cuts at: 1004 1577 2035 2720 1004
Size: 573 458 685 3272
Fragments arranged by size:
3272 685 573 458
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 129 231 1281 1307 2413 2773 129
Size: 102 1050 26 1106 360 2344
Fragments arranged by size:
2344 1106 1050 360 102 26
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 356 1123 1973 2002 356
Size: 767 850 29 3342
Fragments arranged by size:
3342 850 767 29
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 1601 1674 3011 3221 1601
Size: 73 1337 210 3368
Fragments arranged by size:
3368 1337 210 73
SbfI CC_TGCA'GG
Cuts at: 1577 2035 1577
Size: 458 4530
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SfcI C'TryA_G
Cuts at: 33 780 988 1000 1573 2031 2716 3709 3900
Size: 747 208 12 573 458 685 993 191
Cuts at: 3900 33
Size: 1121
Fragments arranged by size:
1121 993 747 685 573 458 208 191
12
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4988
SmlI C'TyrA_G
Cuts at: 600 2143 3058 3550 3812 600
Size: 1543 915 492 262 1776
Fragments arranged by size:
1776 1543 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 306 2041 2541 306
Size: 1735 500 2753
Fragments arranged by size:
2753 1735 500
SspI AAT'ATT
Cuts at: 1932 1932
Size: 4988
StuI AGG'CCT
Cuts at: 1843 1843
Size: 4988
StyI C'CwwG_G
Cuts at: 107 248 1639 2122 2476 107
Size: 141 1391 483 354 2619
Fragments arranged by size:
2619 1391 483 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1181 2571 2704 3163 3372 129
Size: 1052 1390 133 459 209 1745
Fragments arranged by size:
1745 1390 1052 459 209 133
TatI w'GTAC_w
Cuts at: 4503 4503
Size: 4988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 274 306 325 357 636 668 4505
Size: 32 103 32 19 32 279 32 3837
Cuts at: 4505 4537 139
Size: 32 590
Fragments arranged by size:
3837 590 279 103 32 32 32 32
32 19
Tth111I GACn'n_nGTC
Cuts at: 715 2785 715
Size: 2070 2918
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4988
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4988
XmnI GAAnn'nnTTC
Cuts at: 805 805
Size: 4988
ZraI GAC'GTC
Cuts at: 777 1238 777
Size: 461 4527
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI