Restriction map for
pF1KE0993
(Circular) (Six-base) MAPSORT of: pF1KE0993.seq Check: 355 from: 1 to: 5048
pF1KE0993 5048 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1094 1094
Size: 5048
AccI GT'mk_AC
Cuts at: 2086 2086
Size: 5048
Acc65I G'GTAC_C
Cuts at: 2064 2064
Size: 5048
AclI AA'CG_TT
Cuts at: 4888 4888
Size: 5048
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1775 2873 3305 4052 4320 1553
Size: 222 1098 432 747 268 2281
Fragments arranged by size:
2281 1098 747 432 268 222
AflII C'TTAA_G
Cuts at: 286 286
Size: 5048
AflIII A'CryG_T
Cuts at: 1760 2400 3504 4481 1760
Size: 640 1104 977 2327
Fragments arranged by size:
2327 1104 977 640
AgeI A'CCGG_T
Cuts at: 3436 3436
Size: 5048
AhdI GACnn_n'nnGTC
Cuts at: 585 741 753 585
Size: 156 12 4880
Fragments arranged by size:
4880 156 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1246 1916 1246
Size: 670 4378
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2012
Size: 32 5016
AlwNI CAG_nnn'CTG
Cuts at: 534 1095 1263 1842 1958 1980 2001 2353 3920
Size: 561 168 579 116 22 21 352 1567
Cuts at: 3920 534
Size: 1662
Fragments arranged by size:
1662 1567 579 561 352 168 116 22
21
ApaLI G'TGCA_C
Cuts at: 3818 3818
Size: 5048
ApoI r'AATT_y
Cuts at: 115 464 947 1026 2052 4401 4657 115
Size: 349 483 79 1026 2349 256 506
Fragments arranged by size:
2349 1026 506 483 349 256 79
AseI AT'TA_AT
Cuts at: 20 1061 20
Size: 1041 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5048
AvaI C'yCGr_G
Cuts at: 2068 2068
Size: 5048
BamHI G'GATC_C
Cuts at: 658 1079 1636 2073 658
Size: 421 557 437 3633
Fragments arranged by size:
3633 557 437 421
BanI G'GyrC_C
Cuts at: 1941 2064 2478 2726 2761 1941
Size: 123 414 248 35 4228
Fragments arranged by size:
4228 414 248 123 35
BanII G_rGCy'C
Cuts at: 2062 3092 2062
Size: 1030 4018
BbeI G_GCGC'C
Cuts at: 2482 2730 2482
Size: 248 4800
BbsI GAAGACnn'nnnn_
Cuts at: 1025 1341 1523 1763 1025
Size: 316 182 240 4310
Fragments arranged by size:
4310 316 240 182
BbvCI CC'TCA_GC
Cuts at: 1147 1147
Size: 5048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5014
BciVI GTATCCnnnnn_n'
Cuts at: 560 1492 2946 3707 5048 560
Size: 932 1454 761 1341 560
Fragments arranged by size:
1454 1341 932 761 560
BclI T'GATC_A
Cuts at: 2003 2568 2003
Size: 565 4483
BfrBI ATG'CAT
Cuts at: 555 767 799 1552 555
Size: 212 32 753 4051
Fragments arranged by size:
4051 753 212 32
BglII A'GATC_T
Cuts at: 450 1243 2563 4600 450
Size: 793 1320 2037 898
Fragments arranged by size:
2037 1320 898 793
BlpI GC'TnA_GC
Cuts at: 2160 2160
Size: 5048
Bme1580I G_kGCm'C
Cuts at: 1104 2673 2766 3822 1104
Size: 1569 93 1056 2330
Fragments arranged by size:
2330 1569 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2260 2341 2676 4820 2260
Size: 81 335 2144 2488
Fragments arranged by size:
2488 2144 335 81
BmtI G_CTAG'C
Cuts at: 1907 1907
Size: 5048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 5048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2014 1141
Size: 873 4175
Bpu10I CC'TnA_GC
Cuts at: 1147 1147
Size: 5048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 1931 2224 3103 3595 3893 1864
Size: 67 293 879 492 298 3019
Fragments arranged by size:
3019 879 492 298 293 67
BsaI GGTCTCn'nnnn_
Cuts at: 4752 4752
Size: 5048
BsaAI yAC'GTr
Cuts at: 195 2401 195
Size: 2206 2842
BsaBI GATnn'nnATC
Cuts at: 6 2562 4599 6
Size: 2556 2037 455
Fragments arranged by size:
2556 2037 455
BsaHI Gr'CG_yC
Cuts at: 1126 2479 2727 3429 4925 1126
Size: 1353 248 702 1496 1249
Fragments arranged by size:
1496 1353 1249 702 248
BsaWI w'CCGG_w
Cuts at: 2237 2758 3436 3710 3857 4268 2237
Size: 521 678 274 147 411 3017
Fragments arranged by size:
3017 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 675 705 845 875 2012 2042 675
Size: 30 140 30 1137 30 3681
Fragments arranged by size:
3681 1137 140 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 281 343 576 1333 1597 281
Size: 62 233 757 264 3732
Fragments arranged by size:
3732 757 264 233 62
BseYI C'CCAG_C
Cuts at: 372 537 1640 1842 2473 3231 3808 4304 372
Size: 165 1103 202 631 758 577 496 1116
Fragments arranged by size:
1116 1103 758 631 577 496 202 165
BsiEI CG_ry'CG
Cuts at: 104 2590 2636 3844 104
Size: 2486 46 1208 1308
Fragments arranged by size:
2486 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2062 2840 3030 3822 2062
Size: 778 190 792 3288
Fragments arranged by size:
3288 792 778 190
BsmI GAATG_Cn'
Cuts at: 799 1235 1905 799
Size: 436 670 3942
Fragments arranged by size:
3942 670 436
Bsp1286I G_dGCh'C
Cuts at: 1104 2062 2673 2766 2840 3030 3092 3822 1104
Size: 958 611 93 74 190 62 730 2330
Fragments arranged by size:
2330 958 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4268 4268
Size: 5048
BspHI T'CATG_A
Cuts at: 4224 4224
Size: 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1094 1427 2098 2312 2614 2995 1094
Size: 333 671 214 302 381 3147
Fragments arranged by size:
3147 671 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 3340 3394 4878 5043 50
Size: 3290 54 1484 165 55
Fragments arranged by size:
3290 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2960 2960
Size: 5048
BsrFI r'CCGG_y
Cuts at: 3046 3227 3436 3046
Size: 181 209 4658
Fragments arranged by size:
4658 209 181
BsrGI T'GTAC_A
Cuts at: 327 327
Size: 5048
BssSI C'ACGA_G
Cuts at: 3319 3677 3319
Size: 358 4690
BstAPI GCAn_nnn'nTGC
Cuts at: 2425 2425
Size: 5048
BstBI TT'CG_AA
Cuts at: 3409 3409
Size: 5048
BstXI CCAn_nnnn'nTGG
Cuts at: 1698 1698
Size: 5048
BstYI r'GATC_y
Cuts at: 1 450 658 1079 1243 1508 1636 1916 2073
Size: 449 208 421 164 265 128 280 157
Cuts at: 2073 2540 2563 2898 3144 4156 4242 4254 4600
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4600 1
Size: 449
Fragments arranged by size:
1012 467 449 449 421 346 335 280
265 246 208 164 157 128 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 710 1328 710
Size: 618 4430
BtgI C'CryG_G
Cuts at: 1445 1748 1445
Size: 303 4745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2998 3179 3342 2998
Size: 181 163 4704
Fragments arranged by size:
4704 181 163
BtsI GCAGTG_nn'
Cuts at: 222 2291 222
Size: 2069 2979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 383 418 383
Size: 35 5013
DraI TTT'AAA
Cuts at: 833 953 1298 2047 833
Size: 120 345 749 3834
Fragments arranged by size:
3834 749 345 120
DrdI GACnn_nn'nnGTC
Cuts at: 2754 3612 2754
Size: 858 4190
EaeI y'GGCC_r
Cuts at: 671 1688 1796 2633 2807 3198 3225 4570 671
Size: 1017 108 837 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 1017 837 391 174 108 27
EagI C'GGCC_G
Cuts at: 2633 2633
Size: 5048
EarI CTCTTCn'nnn_
Cuts at: 1361 1382 1529 1565 1856 1985 3071 3281 1361
Size: 21 147 36 291 129 1086 210 3128
Fragments arranged by size:
3128 1086 291 210 147 129 36 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3256 3566 3712 4858 3256
Size: 310 146 1146 3446
Fragments arranged by size:
3446 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2060 2060
Size: 5048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1553 1775 2014 2873 3305 4052 4320 1141
Size: 412 222 239 859 432 747 268 1869
Fragments arranged by size:
1869 859 747 432 412 268 239 222
EcoO109I rG'GnC_Cy
Cuts at: 2187 2187
Size: 5048
EcoRI G'AATT_C
Cuts at: 2052 2052
Size: 5048
EcoRV GAT'ATC
Cuts at: 874 1677 874
Size: 803 4245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1358 1540 3067 1358
Size: 182 1527 3339
Fragments arranged by size:
3339 1527 182
FspI TGC'GCA
Cuts at: 2829 2829
Size: 5048
HaeII r_GCGC'y
Cuts at: 2482 2730 3752 4731 2482
Size: 248 1022 979 2799
Fragments arranged by size:
2799 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 558 571 590 603 727 739 759 771 1471
Size: 13 19 13 124 12 20 12 700
Cuts at: 1471 1503 2890 2920 2922 2952 558
Size: 32 1387 30 2 30 2654
Fragments arranged by size:
2654 1387 700 124 32 30 30 20
19 13 13 12 12 2
HincII GTy'rAC
Cuts at: 546 2087 546
Size: 1541 3507
KasI G'GCGC_C
Cuts at: 2478 2726 2478
Size: 248 4800
KpnI G_GTAC'C
Cuts at: 2068 2068
Size: 5048
MfeI C'AATT_G
Cuts at: 1469 1469
Size: 5048
MluI A'CGCG_T
Cuts at: 4481 4481
Size: 5048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 282 725 3719 3903 282
Size: 443 2994 184 1427
Fragments arranged by size:
2994 1427 443 184
MscI TGG'CCA
Cuts at: 673 1690 1798 2809 673
Size: 1017 108 1011 2912
Fragments arranged by size:
2912 1017 1011 108
MslI CAynn'nnrTG
Cuts at: 762 1747 1898 3037 3164 762
Size: 985 151 1139 127 2646
Fragments arranged by size:
2646 1139 985 151 127
MspA1I CmG'CkG
Cuts at: 531 1263 2159 2473 2833 3846 4091 531
Size: 732 896 314 360 1013 245 1488
Fragments arranged by size:
1488 1013 896 732 360 314 245
NaeI GCC'GGC
Cuts at: 3229 3229
Size: 5048
NarI GG'CG_CC
Cuts at: 2479 2727 2479
Size: 248 4800
NcoI C'CATG_G
Cuts at: 1445 1748 1445
Size: 303 4745
NgoMIV G'CCGG_C
Cuts at: 3227 3227
Size: 5048
NheI G'CTAG_C
Cuts at: 1903 1903
Size: 5048
NsiI A_TGCA'T
Cuts at: 557 769 801 1554 557
Size: 212 32 753 4051
Fragments arranged by size:
4051 753 212 32
NspI r_CATG'y
Cuts at: 555 767 909 1764 2101 2601 3508 555
Size: 212 142 855 337 500 907 2095
Fragments arranged by size:
2095 907 855 500 337 212 142
PciI A'CATG_T
Cuts at: 1760 3504 1760
Size: 1744 3304
PmeI GTTT'AAAC
Cuts at: 2047 2047
Size: 5048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 4187 4219 2012
Size: 32 2143 32 2841
Fragments arranged by size:
2841 2143 32 32
PstI C_TGCA'G
Cuts at: 1091 2095 2780 1091
Size: 1004 685 3359
Fragments arranged by size:
3359 1004 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5048
PvuII CAG'CTG
Cuts at: 531 1263 2473 2833 531
Size: 732 1210 360 2746
Fragments arranged by size:
2746 1210 732 360
RsrII CG'GwC_CG
Cuts at: 3243 3243
Size: 5048
SacI G_AGCT'C
Cuts at: 2062 2062
Size: 5048
SalI G'TCGA_C
Cuts at: 2085 2085
Size: 5048
SapI GCTCTTCn'nnn_
Cuts at: 1985 3071 3281 1985
Size: 1086 210 3752
Fragments arranged by size:
3752 1086 210
SbfI CC_TGCA'GG
Cuts at: 2095 2095
Size: 5048
ScaI AGT'ACT
Cuts at: 436 1540 4565 436
Size: 1104 3025 919
Fragments arranged by size:
3025 1104 919
SfcI C'TryA_G
Cuts at: 33 979 1087 2091 2776 3769 3960 33
Size: 946 108 1004 685 993 191 1121
Fragments arranged by size:
1121 1004 993 946 685 191 108
SfoI GGC'GCC
Cuts at: 2480 2728 2480
Size: 248 4800
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5048
SmaI CCC'GGG
Cuts at: 2070 2070
Size: 5048
SmlI C'TyrA_G
Cuts at: 286 1879 1946 2203 3118 3610 3872 286
Size: 1593 67 257 915 492 262 1462
Fragments arranged by size:
1593 1462 915 492 262 257 67
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5048
SphI G_CATG'C
Cuts at: 767 2101 2601 767
Size: 1334 500 3214
Fragments arranged by size:
3214 1334 500
StyI C'CwwG_G
Cuts at: 726 1445 1494 1748 2182 2536 726
Size: 719 49 254 434 354 3238
Fragments arranged by size:
3238 719 434 354 254 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 2631 2764 3223 3432 386
Size: 2245 133 459 209 2002
Fragments arranged by size:
2245 2002 459 209 133
TatI w'GTAC_w
Cuts at: 327 434 1113 1275 1538 1982 4563 327
Size: 107 679 162 263 444 2581 812
Fragments arranged by size:
2581 812 679 444 263 162 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 845 877 4565 4597 845
Size: 32 3688 32 1296
Fragments arranged by size:
3688 1296 32 32
Tth111I GACn'n_nGTC
Cuts at: 2845 2845
Size: 5048
XbaI T'CTAG_A
Cuts at: 1725 2079 1725
Size: 354 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1746 1839 2543 1746
Size: 93 704 4251
Fragments arranged by size:
4251 704 93
XmaI C'CCGG_G
Cuts at: 2068 2068
Size: 5048
XmnI GAAnn'nnTTC
Cuts at: 1338 1338
Size: 5048
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI ApaI AscI AvrII BaeI BglI
BmgBI BsgI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
DraIII EcoNI FseI FspAI HindIII HpaI NdeI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI