Restriction map for
pF1KE1002
(Circular) (Six-base) MAPSORT of: pF1KE1002.seq Check: 9418 from: 1 to: 3560
pF1KE1002 3560 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 598 598
Size: 3560
Acc65I G'GTAC_C
Cuts at: 576 576
Size: 3560
AclI AA'CG_TT
Cuts at: 3400 3400
Size: 3560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1385 1817 2564 2832 146
Size: 1239 432 747 268 874
Fragments arranged by size:
1239 874 747 432 268
AflIII A'CryG_T
Cuts at: 912 2016 2993 912
Size: 1104 977 1479
Fragments arranged by size:
1479 1104 977
AgeI A'CCGG_T
Cuts at: 1948 1948
Size: 3560
AlwNI CAG_nnn'CTG
Cuts at: 865 2432 865
Size: 1567 1993
ApaLI G'TGCA_C
Cuts at: 2330 2330
Size: 3560
ApoI r'AATT_y
Cuts at: 142 310 564 2913 3169 142
Size: 168 254 2349 256 533
Fragments arranged by size:
2349 533 256 254 168
AseI AT'TA_AT
Cuts at: 20 266 20
Size: 246 3314
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
AvaI C'yCGr_G
Cuts at: 580 580
Size: 3560
BamHI G'GATC_C
Cuts at: 585 585
Size: 3560
BanI G'GyrC_C
Cuts at: 576 990 1238 1273 576
Size: 414 248 35 2863
Fragments arranged by size:
2863 414 248 35
BanII G_rGCy'C
Cuts at: 574 1604 574
Size: 1030 2530
BbeI G_GCGC'C
Cuts at: 994 1242 994
Size: 248 3312
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2219 3560 1458
Size: 761 1341 1458
Fragments arranged by size:
1458 1341 761
BclI T'GATC_A
Cuts at: 482 1080 482
Size: 598 2962
BglII A'GATC_T
Cuts at: 220 1075 3112 220
Size: 855 2037 668
Fragments arranged by size:
2037 855 668
BlpI GC'TnA_GC
Cuts at: 672 672
Size: 3560
Bme1580I G_kGCm'C
Cuts at: 1185 1278 2334 1185
Size: 93 1056 2411
Fragments arranged by size:
2411 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 772 853 1188 3332 772
Size: 81 335 2144 1000
Fragments arranged by size:
2144 1000 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1615 2107 2405 736
Size: 879 492 298 1891
Fragments arranged by size:
1891 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3264 3264
Size: 3560
BsaAI yAC'GTr
Cuts at: 913 913
Size: 3560
BsaBI GATnn'nnATC
Cuts at: 6 1074 3111 6
Size: 1068 2037 455
Fragments arranged by size:
2037 1068 455
BsaHI Gr'CG_yC
Cuts at: 991 1239 1941 3437 991
Size: 248 702 1496 1114
Fragments arranged by size:
1496 1114 702 248
BsaWI w'CCGG_w
Cuts at: 749 1270 1948 2222 2369 2780 749
Size: 521 678 274 147 411 1529
Fragments arranged by size:
1529 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 985 1743 2320 2816 985
Size: 758 577 496 1729
Fragments arranged by size:
1729 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1102 1148 2356 104
Size: 998 46 1208 1308
Fragments arranged by size:
1308 1208 998 46
BsiHKAI G_wGCw'C
Cuts at: 574 1352 1542 2334 574
Size: 778 190 792 1800
Fragments arranged by size:
1800 792 778 190
BsmI GAATG_Cn'
Cuts at: 373 373
Size: 3560
Bsp1286I G_dGCh'C
Cuts at: 574 1185 1278 1352 1542 1604 2334 574
Size: 611 93 74 190 62 730 1800
Fragments arranged by size:
1800 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2780 2780
Size: 3560
BspHI T'CATG_A
Cuts at: 2736 2736
Size: 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 824 1126 1507 610
Size: 214 302 381 2663
Fragments arranged by size:
2663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1852 1906 3390 3555 50
Size: 1802 54 1484 165 55
Fragments arranged by size:
1802 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 405 1472 405
Size: 1067 2493
BsrFI r'CCGG_y
Cuts at: 1558 1739 1948 1558
Size: 181 209 3170
Fragments arranged by size:
3170 209 181
BsrGI T'GTAC_A
Cuts at: 534 534
Size: 3560
BssSI C'ACGA_G
Cuts at: 1831 2189 1831
Size: 358 3202
BstAPI GCAn_nnn'nTGC
Cuts at: 937 937
Size: 3560
BstBI TT'CG_AA
Cuts at: 1921 1921
Size: 3560
BstYI r'GATC_y
Cuts at: 1 220 585 1052 1075 1410 1656 2668 2754
Size: 219 365 467 23 335 246 1012 86
Cuts at: 2754 2766 3112 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 365 346 335 246 219
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1510 1691 1854 1510
Size: 181 163 3216
Fragments arranged by size:
3216 181 163
BtsI GCAGTG_nn'
Cuts at: 803 803
Size: 3560
DraI TTT'AAA
Cuts at: 559 559
Size: 3560
DrdI GACnn_nn'nnGTC
Cuts at: 1266 2124 1266
Size: 858 2702
EaeI y'GGCC_r
Cuts at: 1145 1319 1710 1737 3082 1145
Size: 174 391 27 1345 1623
Fragments arranged by size:
1623 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1145 1145
Size: 3560
EarI CTCTTCn'nnn_
Cuts at: 122 521 1583 1793 122
Size: 399 1062 210 1889
Fragments arranged by size:
1889 1062 399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1768 2078 2224 3370 1768
Size: 310 146 1146 1958
Fragments arranged by size:
1958 1146 310 146
EcoICRI GAG'CTC
Cuts at: 572 572
Size: 3560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1385 1817 2564 2832 146
Size: 1239 432 747 268 874
Fragments arranged by size:
1239 874 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 699 699
Size: 3560
EcoRI G'AATT_C
Cuts at: 142 564 142
Size: 422 3138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1579 1579
Size: 3560
FspI TGC'GCA
Cuts at: 1341 1341
Size: 3560
HaeII r_GCGC'y
Cuts at: 994 1242 2264 3243 994
Size: 248 1022 979 1311
Fragments arranged by size:
1311 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1402 1432 1434 1464 1402
Size: 30 2 30 3498
Fragments arranged by size:
3498 30 30 2
HincII GTy'rAC
Cuts at: 458 599 458
Size: 141 3419
HindIII A'AGCT_T
Cuts at: 152 152
Size: 3560
HpaI GTT'AAC
Cuts at: 458 458
Size: 3560
KasI G'GCGC_C
Cuts at: 990 1238 990
Size: 248 3312
KpnI G_GTAC'C
Cuts at: 580 580
Size: 3560
MfeI C'AATT_G
Cuts at: 296 296
Size: 3560
MluI A'CGCG_T
Cuts at: 2993 2993
Size: 3560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2415 2231
Size: 184 3376
MscI TGG'CCA
Cuts at: 1321 1321
Size: 3560
MslI CAynn'nnrTG
Cuts at: 1549 1676 1549
Size: 127 3433
MspA1I CmG'CkG
Cuts at: 671 985 1345 2358 2603 671
Size: 314 360 1013 245 1628
Fragments arranged by size:
1628 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1741 1741
Size: 3560
NarI GG'CG_CC
Cuts at: 991 1239 991
Size: 248 3312
NcoI C'CATG_G
Cuts at: 107 107
Size: 3560
NgoMIV G'CCGG_C
Cuts at: 1739 1739
Size: 3560
NspI r_CATG'y
Cuts at: 613 1113 2020 613
Size: 500 907 2153
Fragments arranged by size:
2153 907 500
PciI A'CATG_T
Cuts at: 2016 2016
Size: 3560
PmeI GTTT'AAAC
Cuts at: 559 559
Size: 3560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 3528
PstI C_TGCA'G
Cuts at: 420 607 1292 420
Size: 187 685 2688
Fragments arranged by size:
2688 685 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3560
PvuII CAG'CTG
Cuts at: 985 1345 985
Size: 360 3200
RsrII CG'GwC_CG
Cuts at: 1755 1755
Size: 3560
SacI G_AGCT'C
Cuts at: 574 574
Size: 3560
SalI G'TCGA_C
Cuts at: 597 597
Size: 3560
SapI GCTCTTCn'nnn_
Cuts at: 122 1583 1793 122
Size: 1461 210 1889
Fragments arranged by size:
1889 1461 210
SbfI CC_TGCA'GG
Cuts at: 607 607
Size: 3560
ScaI AGT'ACT
Cuts at: 3077 3077
Size: 3560
SfcI C'TryA_G
Cuts at: 33 416 603 1288 2281 2472 33
Size: 383 187 685 993 191 1121
Fragments arranged by size:
1121 993 685 383 191 187
SfoI GGC'GCC
Cuts at: 992 1240 992
Size: 248 3312
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
SmaI CCC'GGG
Cuts at: 582 582
Size: 3560
SmlI C'TyrA_G
Cuts at: 715 1630 2122 2384 715
Size: 915 492 262 1891
Fragments arranged by size:
1891 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3560
SphI G_CATG'C
Cuts at: 613 1113 613
Size: 500 3060
StyI C'CwwG_G
Cuts at: 107 444 694 1048 107
Size: 337 250 354 2619
Fragments arranged by size:
2619 354 337 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1143 1276 1735 1944 1143
Size: 133 459 209 2759
Fragments arranged by size:
2759 459 209 133
TatI w'GTAC_w
Cuts at: 534 3075 534
Size: 2541 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3528
Tth111I GACn'n_nGTC
Cuts at: 1357 1357
Size: 3560
XbaI T'CTAG_A
Cuts at: 591 591
Size: 3560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1055 1055
Size: 3560
XmaI C'CCGG_G
Cuts at: 580 580
Size: 3560
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI