Restriction map for
pF1KE1005
(Circular) (Six-base) MAPSORT of: pF1KE1005.seq Check: 2936 from: 1 to: 3671
pF1KE1005 3671 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 463 463
Size: 3671
AccI GT'mk_AC
Cuts at: 335 709 335
Size: 374 3297
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 604 675 3024 3280 604
Size: 71 2349 256 995
Fragments arranged by size:
2349 995 256 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 134 307 502 691 134
Size: 173 195 189 3114
Fragments arranged by size:
3114 195 189 173
BamHI G'GATC_C
Cuts at: 353 696 353
Size: 343 3328
BanI G'GyrC_C
Cuts at: 178 687 1101 1349 1384 178
Size: 509 414 248 35 2465
Fragments arranged by size:
2465 509 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BbsI GAAGACnn'nnnn_
Cuts at: 482 521 482
Size: 39 3632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3637
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3671
BglI GCCn_nnn'nGGC
Cuts at: 130 442 130
Size: 312 3359
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 181 205 1296 1389 2445 181
Size: 24 1091 93 1056 1407
Fragments arranged by size:
1407 1091 1056 93 24
BmgBI CAC'GTC
Cuts at: 315 315
Size: 3671
BmrI ACTGGGnnnn_n'
Cuts at: 352 883 964 1299 3443 352
Size: 531 81 335 2144 580
Fragments arranged by size:
2144 580 531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 529
Size: 3671
Bpu10I CC'TnA_GC
Cuts at: 342 342
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 639 1024 639
Size: 385 3286
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 585 860 1381 2059 2333 2480 2891 585
Size: 275 521 678 274 147 411 1365
Fragments arranged by size:
1365 678 521 411 275 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 488 520 532 295
Size: 193 32 12 3434
Fragments arranged by size:
3434 193 32 12
BseYI C'CCAG_C
Cuts at: 231 398 561 1096 1854 2431 2927 231
Size: 167 163 535 758 577 496 975
Fragments arranged by size:
975 758 577 535 496 167 163
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 181 205 685 1296 1389 1463 1653 1715 2445
Size: 24 480 611 93 74 190 62 730
Cuts at: 2445 181
Size: 1407
Fragments arranged by size:
1407 730 611 480 190 93 74 62
24
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 463 721 935 1237 1618 463
Size: 258 214 302 381 2516
Fragments arranged by size:
2516 381 302 258 214
BsrBI CCG'CTC
Cuts at: 50 303 341 601 1963 2017 3501 3666 50
Size: 253 38 260 1362 54 1484 165 55
Fragments arranged by size:
1484 1362 260 253 165 55 54 38
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 353 486 696 1163 1186 1521 1767 2779
Size: 352 133 210 467 23 335 246 1012
Cuts at: 2779 2865 2877 3223 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 352 346 335 246 210
133 86 23 12
BtgI C'CryG_G
Cuts at: 128 405 128
Size: 277 3394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 914 914
Size: 3671
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DraIII CAC_nnn'GTG
Cuts at: 185 411 185
Size: 226 3445
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 119 645 1256 1430 1821 1848 3193 119
Size: 526 611 174 391 27 1345 597
Fragments arranged by size:
1345 611 597 526 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1496 1928 2675 2943 529
Size: 967 432 747 268 1257
Fragments arranged by size:
1257 967 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 544 544
Size: 3671
EcoO109I rG'GnC_Cy
Cuts at: 425 810 425
Size: 385 3286
EcoRI G'AATT_C
Cuts at: 604 675 604
Size: 71 3600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 232 405 1105 1353 2375 3354 232
Size: 173 700 248 1022 979 549
Fragments arranged by size:
1022 979 700 549 248 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 647 1432 647
Size: 785 2886
MslI CAynn'nnrTG
Cuts at: 464 1660 1787 464
Size: 1196 127 2348
Fragments arranged by size:
2348 1196 127
MspA1I CmG'CkG
Cuts at: 351 782 1096 1456 2469 2714 351
Size: 431 314 360 1013 245 1308
Fragments arranged by size:
1308 1013 431 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 128 128
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3671
NspI r_CATG'y
Cuts at: 115 525 724 1224 2131 115
Size: 410 199 500 907 1655
Fragments arranged by size:
1655 907 500 410 199
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 364 411 364
Size: 47 3624
PfoI T'CCnGG_A
Cuts at: 545 545
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 167 718 1403 167
Size: 551 685 2435
Fragments arranged by size:
2435 685 551
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SexAI A'CCwGG_T
Cuts at: 181 181
Size: 3671
SfcI C'TryA_G
Cuts at: 33 163 714 1399 2392 2583 33
Size: 130 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 191 130
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 136 309 693 136
Size: 173 384 3114
Fragments arranged by size:
3114 384 173
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 128 805 1159 128
Size: 677 354 2640
Fragments arranged by size:
2640 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1166 114
Size: 1052 2619
XmaI C'CCGG_G
Cuts at: 134 307 691 134
Size: 173 384 3114
Fragments arranged by size:
3114 384 173
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI