Restriction map for
pF1KE1013
(Circular) (Six-base) MAPSORT of: pF1KE1013.seq Check: 6214 from: 1 to: 3968
pF1KE1013 3968 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 392 1006 392
Size: 614 3354
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 2225 2972 3240 1793
Size: 432 747 268 2521
Fragments arranged by size:
2521 747 432 268
AflIII A'CryG_T
Cuts at: 470 1320 2424 3401 470
Size: 850 1104 977 1037
Fragments arranged by size:
1104 1037 977 850
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AhdI GACnn_n'nnGTC
Cuts at: 529 529
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 1273 2840 1273
Size: 1567 2401
ApaLI G'TGCA_C
Cuts at: 457 2738 457
Size: 2281 1687
ApoI r'AATT_y
Cuts at: 972 3321 3577 972
Size: 2349 256 1363
Fragments arranged by size:
2349 1363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 295 988 295
Size: 693 3275
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 984 1398 1646 1681 984
Size: 414 248 35 3271
Fragments arranged by size:
3271 414 248 35
BanII G_rGCy'C
Cuts at: 758 982 2012 758
Size: 224 1030 2714
Fragments arranged by size:
2714 1030 224
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BbvCI CC'TCA_GC
Cuts at: 721 721
Size: 3968
BciVI GTATCCnnnnn_n'
Cuts at: 636 1866 2627 3968 636
Size: 1230 761 1341 636
Fragments arranged by size:
1341 1230 761 636
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 159 461 605 740 1593 1686 2742 159
Size: 302 144 135 853 93 1056 1385
Fragments arranged by size:
1385 1056 853 302 144 135 93
BmgBI CAC'GTC
Cuts at: 786 786
Size: 3968
BmrI ACTGGGnnnn_n'
Cuts at: 1180 1261 1596 3740 1180
Size: 81 335 2144 1408
Fragments arranged by size:
2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 874 946 366
Size: 508 72 3388
Fragments arranged by size:
3388 508 72
Bpu10I CC'TnA_GC
Cuts at: 721 721
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 698 830 1399 1647 2349 3845 698
Size: 132 569 248 702 1496 821
Fragments arranged by size:
1496 821 702 569 248 132
BsaWI w'CCGG_w
Cuts at: 1157 1678 2356 2630 2777 3188 1157
Size: 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 137 157 160 328 134
Size: 3 20 3 168 3774
Fragments arranged by size:
3774 168 20 3 3
BseYI C'CCAG_C
Cuts at: 1393 2151 2728 3224 1393
Size: 758 577 496 2137
Fragments arranged by size:
2137 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 916 132
Size: 784 3184
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 296 461 713 758 982 1760 1950 2742 296
Size: 165 252 45 224 778 190 792 1522
Fragments arranged by size:
1522 792 778 252 224 190 165 45
BsmI GAATG_Cn'
Cuts at: 419 419
Size: 3968
BsmBI CGTCTCn'nnnn_
Cuts at: 850 850
Size: 3968
Bsp1286I G_dGCh'C
Cuts at: 159 296 461 605 713 740 758 982 1593
Size: 137 165 144 108 27 18 224 611
Cuts at: 1593 1686 1760 1950 2012 2742 159
Size: 93 74 190 62 730 1385
Fragments arranged by size:
1385 730 611 224 190 165 144 137
108 93 74 62 27 18
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 593 3144 593
Size: 2551 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1018 1232 1534 1915 1018
Size: 214 302 381 3071
Fragments arranged by size:
3071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 891 2260 2314 3798 3963 50
Size: 841 1369 54 1484 165 55
Fragments arranged by size:
1484 1369 841 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 660 1966 2147 2356 660
Size: 1306 181 209 2272
Fragments arranged by size:
2272 1306 209 181
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstYI r'GATC_y
Cuts at: 1 993 1460 1483 1818 2064 3076 3162 3174
Size: 992 467 23 335 246 1012 86 12
Cuts at: 3174 3520 1
Size: 346 449
Fragments arranged by size:
1012 992 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 325 1211 325
Size: 886 3082
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 690 821 1553 1727 2118 2145 3490 690
Size: 131 732 174 391 27 1345 1168
Fragments arranged by size:
1345 1168 732 391 174 131 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 625 863 1991 2201 625
Size: 238 1128 210 2392
Fragments arranged by size:
2392 1128 238 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 756 980 756
Size: 224 3744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 874 946 1793 2225 2972 3240 366
Size: 508 72 847 432 747 268 1094
Fragments arranged by size:
1094 847 747 508 432 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 622 622
Size: 3968
EcoO109I rG'GnC_Cy
Cuts at: 168 244 354 879 1107 168
Size: 76 110 525 228 3029
Fragments arranged by size:
3029 525 228 110 76
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3968
FspI TGC'GCA
Cuts at: 135 1749 135
Size: 1614 2354
HaeII r_GCGC'y
Cuts at: 1402 1650 2672 3651 1402
Size: 248 1022 979 1719
Fragments arranged by size:
1719 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 515 547 1810 1840 1842 1872 364
Size: 32 119 32 1263 30 2 30 2460
Fragments arranged by size:
2460 1263 119 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1007 1007
Size: 3968
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2639 2823 2639
Size: 184 3784
MscI TGG'CCA
Cuts at: 1729 1729
Size: 3968
MslI CAynn'nnrTG
Cuts at: 598 1957 2084 598
Size: 1359 127 2482
Fragments arranged by size:
2482 1359 127
MspA1I CmG'CkG
Cuts at: 495 1079 1393 1753 2766 3011 495
Size: 584 314 360 1013 245 1452
Fragments arranged by size:
1452 1013 584 360 314 245
NaeI GCC'GGC
Cuts at: 662 2149 662
Size: 1487 2481
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NgoMIV G'CCGG_C
Cuts at: 660 2147 660
Size: 1487 2481
NspI r_CATG'y
Cuts at: 936 1021 1521 2428 936
Size: 85 500 907 2476
Fragments arranged by size:
2476 907 500 85
PasI CC'CwG_GG
Cuts at: 159 159
Size: 3968
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PfoI T'CCnGG_A
Cuts at: 623 779 623
Size: 156 3812
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 695 727 3107 3139 695
Size: 32 2380 32 1524
Fragments arranged by size:
2380 1524 32 32
PpuMI rG'GwC_Cy
Cuts at: 244 354 244
Size: 110 3858
PstI C_TGCA'G
Cuts at: 332 1015 1700 332
Size: 683 685 2600
Fragments arranged by size:
2600 685 683
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 1393 1753 1393
Size: 360 3608
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 758 982 758
Size: 224 3744
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SanDI GG'GwC_CC
Cuts at: 244 244
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 3485 3485
Size: 3968
SfcI C'TryA_G
Cuts at: 33 328 1011 1696 2689 2880 33
Size: 295 683 685 993 191 1121
Fragments arranged by size:
1121 993 685 683 295 191
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StyI C'CwwG_G
Cuts at: 1102 1456 1102
Size: 354 3614
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1551 1684 2143 2352 1551
Size: 133 459 209 3167
Fragments arranged by size:
3167 459 209 133
TatI w'GTAC_w
Cuts at: 3483 3483
Size: 3968
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 3936
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 1463 522
Size: 941 3027
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
XmnI GAAnn'nnTTC
Cuts at: 307 472 307
Size: 165 3803
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI