Restriction map for
pF1KE1015
(Circular) (Six-base) MAPSORT of: pF1KE1015.seq Check: 2654 from: 1 to: 4103
pF1KE1015 4103 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1928 2360 3107 3375 592
Size: 1336 432 747 268 1320
Fragments arranged by size:
1336 1320 747 432 268
AflIII A'CryG_T
Cuts at: 153 1455 2559 3536 153
Size: 1302 1104 977 720
Fragments arranged by size:
1302 1104 977 720
AgeI A'CCGG_T
Cuts at: 668 2491 668
Size: 1823 2280
AlwNI CAG_nnn'CTG
Cuts at: 1408 2975 1408
Size: 1567 2536
ApaI G_GGCC'C
Cuts at: 1032 1032
Size: 4103
ApaLI G'TGCA_C
Cuts at: 339 2873 339
Size: 2534 1569
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 143 776 1043 1128 143
Size: 633 267 85 3118
Fragments arranged by size:
3118 633 267 85
BanI G'GyrC_C
Cuts at: 247 1002 1119 1533 1781 1816 247
Size: 755 117 414 248 35 2534
Fragments arranged by size:
2534 755 414 248 117 35
BanII G_rGCy'C
Cuts at: 182 273 293 586 1032 1117 2147 182
Size: 91 20 293 446 85 1030 2138
Fragments arranged by size:
2138 1030 446 293 91 85 20
BbeI G_GCGC'C
Cuts at: 1006 1537 1785 1006
Size: 531 248 3324
Fragments arranged by size:
3324 531 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 740 706
Size: 34 4069
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 934 955 1036 934
Size: 21 81 4001
Fragments arranged by size:
4001 81 21
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 250 343 1032 1728 1821 2877 250
Size: 93 689 696 93 1056 1476
Fragments arranged by size:
1476 1056 696 689 93 93
BmrI ACTGGGnnnn_n'
Cuts at: 360 1315 1396 1731 3875 360
Size: 955 81 335 2144 588
Fragments arranged by size:
2144 955 588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 196 2470 196
Size: 2274 1829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1003 1054 472
Size: 531 51 3521
Fragments arranged by size:
3521 531 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1279 2158 2650 2948 718
Size: 561 879 492 298 1873
Fragments arranged by size:
1873 879 561 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 322 3807 322
Size: 3485 618
BsaAI yAC'GTr
Cuts at: 593 1456 593
Size: 863 3240
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 524 1003 1534 1782 2484 3980 524
Size: 479 531 248 702 1496 647
Fragments arranged by size:
1496 702 647 531 479 248
BsaWI w'CCGG_w
Cuts at: 221 668 1292 1813 2491 2765 2912 3323 221
Size: 447 624 521 678 274 147 411 1001
Fragments arranged by size:
1001 678 624 521 447 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 856 188
Size: 668 3435
BseYI C'CCAG_C
Cuts at: 542 993 1528 2286 2863 3359 542
Size: 451 535 758 577 496 1286
Fragments arranged by size:
1286 758 577 535 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 214
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 938 1645 1691 2899 104
Size: 834 707 46 1208 1308
Fragments arranged by size:
1308 1208 834 707 46
BsiHKAI G_wGCw'C
Cuts at: 182 293 343 1117 1895 2085 2877 182
Size: 111 50 774 778 190 792 1408
Fragments arranged by size:
1408 792 778 774 190 111 50
BsmI GAATG_Cn'
Cuts at: 951 951
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 182 250 273 293 343 586 1032 1117 1728
Size: 68 23 20 50 243 446 85 611
Cuts at: 1728 1821 1895 2085 2147 2877 182
Size: 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 446 243 190 93 85
74 68 62 50 23 20
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 749 1153 1367 1669 2050 749
Size: 404 214 302 381 2802
Fragments arranged by size:
2802 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 2015 722
Size: 1293 2810
BsrFI r'CCGG_y
Cuts at: 668 2101 2282 2491 668
Size: 1433 181 209 2280
Fragments arranged by size:
2280 1433 209 181
BssSI C'ACGA_G
Cuts at: 272 2374 2732 272
Size: 2102 358 1643
Fragments arranged by size:
2102 1643 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 981 981
Size: 4103
BstYI r'GATC_y
Cuts at: 1 143 776 1043 1085 1128 1595 1618 1953
Size: 142 633 267 42 43 467 23 335
Cuts at: 1953 2199 3211 3297 3309 3655 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 633 467 449 346 335 267 246
142 86 43 42 23 12
BtgI C'CryG_G
Cuts at: 107 284 607 107
Size: 177 323 3603
Fragments arranged by size:
3603 323 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 105 414 2053 2234 2397 105
Size: 309 1639 181 163 1811
Fragments arranged by size:
1811 1639 309 181 163
BtsI GCAGTG_nn'
Cuts at: 465 647 864 1346 465
Size: 182 217 482 3222
Fragments arranged by size:
3222 482 217 182
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 898 1809 2667 898
Size: 911 858 2334
Fragments arranged by size:
2334 911 858
EaeI y'GGCC_r
Cuts at: 410 935 1688 1862 2253 2280 3625 410
Size: 525 753 174 391 27 1345 888
Fragments arranged by size:
1345 888 753 525 391 174 27
EagI C'GGCC_G
Cuts at: 935 1688 935
Size: 753 3350
EarI CTCTTCn'nnn_
Cuts at: 620 876 1055 2126 2336 620
Size: 256 179 1071 210 2387
Fragments arranged by size:
2387 1071 256 210 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2311 2621 2767 3913 127
Size: 2184 310 146 1146 317
Fragments arranged by size:
2184 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 180 291 1115 180
Size: 111 824 3168
Fragments arranged by size:
3168 824 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 592 1003 1054 1928 2360 3107 3375 472
Size: 120 411 51 874 432 747 268 1200
Fragments arranged by size:
1200 874 747 432 411 268 120 51
EcoO109I rG'GnC_Cy
Cuts at: 444 603 764 1029 1242 444
Size: 159 161 265 213 3305
Fragments arranged by size:
3305 265 213 161 159
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
EcoRV GAT'ATC
Cuts at: 117 117
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 927 2122 927
Size: 1195 2908
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 1006 1537 1785 2807 3786 1006
Size: 531 248 1022 979 1323
Fragments arranged by size:
1323 1022 979 531 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 1945 1975 1977 2007 853
Size: 32 1060 30 2 30 2949
Fragments arranged by size:
2949 1060 32 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1002 1533 1781 1002
Size: 531 248 3324
Fragments arranged by size:
3324 531 248
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 412 1864 412
Size: 1452 2651
MslI CAynn'nnrTG
Cuts at: 433 565 2092 2219 433
Size: 132 1527 127 2317
Fragments arranged by size:
2317 1527 132 127
MspA1I CmG'CkG
Cuts at: 405 462 783 804 1214 1528 1888 2901 3146
Size: 57 321 21 410 314 360 1013 245
Cuts at: 3146 405
Size: 1362
Fragments arranged by size:
1362 1013 410 360 321 314 245 57
21
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1003 1534 1782 1003
Size: 531 248 3324
Fragments arranged by size:
3324 531 248
NcoI C'CATG_G
Cuts at: 107 284 607 107
Size: 177 323 3603
Fragments arranged by size:
3603 323 177
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NspI r_CATG'y
Cuts at: 391 691 1156 1656 2563 391
Size: 300 465 500 907 1931
Fragments arranged by size:
1931 907 500 465 300
PasI CC'CwG_GG
Cuts at: 504 504
Size: 4103
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PfoI T'CCnGG_A
Cuts at: 131 182 131
Size: 51 4052
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 444 603 764 444
Size: 159 161 3783
Fragments arranged by size:
3783 161 159
PspOMI G'GGCC_C
Cuts at: 1028 1028
Size: 4103
PstI C_TGCA'G
Cuts at: 533 746 1150 1835 533
Size: 213 404 685 2801
Fragments arranged by size:
2801 685 404 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 783 1528 1888 783
Size: 745 360 2998
Fragments arranged by size:
2998 745 360
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 182 293 1117 182
Size: 111 824 3168
Fragments arranged by size:
3168 824 111
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SanDI GG'GwC_CC
Cuts at: 764 764
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 876 2126 2336 876
Size: 1250 210 2643
Fragments arranged by size:
2643 1250 210
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 712 847 3620 712
Size: 135 2773 1195
Fragments arranged by size:
2773 1195 135
SfcI C'TryA_G
Cuts at: 33 529 742 1146 1831 2824 3015 33
Size: 496 213 404 685 993 191 1121
Fragments arranged by size:
1121 993 685 496 404 213 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 934 934
Size: 4103
SfoI GGC'GCC
Cuts at: 1004 1535 1783 1004
Size: 531 248 3324
Fragments arranged by size:
3324 531 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 733 1258 2173 2665 2927 733
Size: 525 915 492 262 1909
Fragments arranged by size:
1909 915 525 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 907 907
Size: 4103
StyI C'CwwG_G
Cuts at: 107 203 284 607 1237 1591 107
Size: 96 81 323 630 354 2619
Fragments arranged by size:
2619 630 354 323 96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 710 845 3618 710
Size: 135 2773 1195
Fragments arranged by size:
2773 1195 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 3620 3652 856
Size: 32 2732 32 1307
Fragments arranged by size:
2732 1307 32 32
Tth111I GACn'n_nGTC
Cuts at: 898 1900 898
Size: 1002 3101
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI